Review
Biotechnology & Applied Microbiology
Danielle Yarbrough, Sharon Gerecht
Summary: Vascular smooth muscle cells play a vital role in regulating blood pressure and maintaining cardiovascular health. Changes in their surrounding environment can lead to alterations in phenotype and secretory profile, causing pathological conditions. Understanding the interaction between smooth muscle cells and the extracellular matrix is crucial for treating vascular diseases.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Agustina Setiawati, Sungwoo Jeong, Albertus Ivan Brilian, Sang Ho Lee, Jin-Gon Shim, Kwang-Hwan Jung, Kwanwoo Shin
Summary: In this study, a hybrid extracellular matrix (ECM) scaffold was successfully fabricated, and it was demonstrated that the fibronectin (FN)-rich hybrid ECM scaffold promoted successful settling of liver cells. This method holds promise for the fabrication of scaffolds that provide tailored cellular microenvironment for specific organs and can serve as novel pathways for organ replacement or regeneration.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Joao Carlos Ribeiro-Silva, Patricia Nolasco, Jose Eduardo Krieger, Ayumi Aurea Miyakawa
Summary: Vascular aging is characterized by the fragmentation of elastic fibers and collagen deposition, leading to reduced distensibility and increased stiffness in the blood vessels. Research shows that aging promotes changes in smooth muscle cell phenotypes, ultimately resulting in mechanical impairment of aging arteries. Understanding the role of ECM changes during aging may help in developing new strategies to prevent or delay arterial aging and cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Review
Biotechnology & Applied Microbiology
Eugenia Carraro, Lucia Rossi, Edoardo Maghin, Marcella Canton, Martina Piccoli
Summary: This review discusses the importance of generating human skeletal muscle three-dimensional models through tissue engineering approaches, including the study of the most severe myopathies and the comparison of different in vitro models, as well as their effectiveness in simulating disease mechanisms and investigating therapeutic effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Jahnavi Mudigonda, Dongyang Xu, Alan Amedi, Brooks A. Lane, Daniella Corporan, Vivian Wang, Muralidhar Padala
Summary: Encapsulating a decellularized matrix with a polymeric nanofiber coating shows promising attributes for use as a cardiovascular tissue substitute, with good biocompatibility and mechanical properties.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cell & Tissue Engineering
Jie Su, Ling Guo, Chuanyue Wu
Summary: The PINCH-1 and Notch2 signaling axis is critically involved in mediating the effect of ECM stiffness on smooth muscle differentiation of human placental MSCs. Notch2 interacts directly with PINCH-1 to respond to ECM stiffness and promote smooth muscle cell differentiation.
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Article
Polymer Science
Bryan Falcones, Hector Sanz-Fraile, Esther Marhuenda, Irene Mendizabal, Ignacio Cabrera-Aguilera, Nanthilde Malandain, Juan J. Uriarte, Isaac Almendros, Daniel Navajas, Daniel J. Weiss, Ramon Farre, Jorge Otero
Summary: Mesenchymal stromal cell (MSC) therapy for acute respiratory diseases relies on MSCs' secretion of paracrine factors. Preconditioning the MSCs by growing them in a lung-derived extracellular matrix (ECM) can enhance their therapeutic efficacy. Bioink based on decellularized porcine lung ECM hydrogels supports 3D culturing of lung-resident MSCs without additional crosslinking, leading to improved cell-matrix interactions and increased expression of key receptors. This approach shows promise for enhancing MSC preconditioning for therapeutic applications.
Article
Biochemistry & Molecular Biology
Silvia Barbon, Elena Stocco, Martina Contran, Federico Facchin, Rafael Boscolo-Berto, Silvia Todros, Deborah Sandrin, Filippo Romanato, Piero Pavan, Veronica Macchi, Vincenzo Vindigni, Franco Bassetto, Raffaele De Caro, Andrea Porzionato
Summary: This study considers the use of cellular human diaphragm as a scaffold material for the treatment of volumetric muscle loss (VML). After decellularization through four detergent-enzymatic protocols, cells, DNA, and muscle fibers were effectively removed, while collagen/elastin and the majority of the glycosaminoglycan component were preserved. Adipose-derived stem cells cultured with decellularized samples demonstrated high viability, indicating the biosafety of the scaffolds. Acellular diaphragmatic grafts treated with SDS+Tergitol (TM) showed better collagen preservation and integrated well with host tissue upon implantation in mice.
Article
Biochemistry & Molecular Biology
Daniele Boso, Eugenia Carraro, Edoardo Maghin, Silvia Todros, Arben Dedja, Monica Giomo, Nicola Elvassore, Paolo De Coppi, Piero Giovanni Pavan, Martina Piccoli
Summary: Hydrogels, particularly those derived from decellularized extracellular matrix (dECM), show superior properties in mimicking native tissue composition and promoting tissue regeneration. A porcine diaphragmatic dECM-derived hydrogel has been developed and characterized for diaphragmatic applications, demonstrating potential as a useful biological product for diaphragmatic muscle defect repair.
Article
Biochemistry & Molecular Biology
Stefania Croce, Lorenzo Cobianchi, Tamara Zoro, Francesca Dal Mas, Antonia Icaro Cornaglia, Elisa Lenta, Gloria Acquafredda, Annalisa De Silvestri, Maria Antonietta Avanzini, Livia Visai, Szandra Brambilla, Giovanna Bruni, Giulia Di Gravina, Andrea Pietrabissa, Luca Ansaloni, Andrea Peloso
Summary: This study investigates the effect of extracellular matrix (ECM) on the differentiation of porcine mesenchymal stromal cells (pMSCs) into hepatocyte-like cells (HLCs) through decellularization and reseeding. The results suggest that ECM may promote the differentiation of pMSCs into HLCs, but contact with liver ECM alone is not sufficient for complete differentiation.
Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Engineering, Biomedical
Tomoyuki Kojima, Takashi Nakamura, Junichi Saito, Yuko Hidaka, Taisuke Akimoto, Hana Inoue, Christian Nanga Chick, Toyonobu Usuki, Makoto Kaneko, Etsuko Miyagi, Yoshihiro Ishikawa, Utako Yokoyama
Summary: Periodic hydrostatic pressurization under hypoxia enables the fabrication of scaffoldless human vascular mimetics with a spatial arrangement of cells and matrices. These mimetics show excellent mechanical properties and biocompatibility, making them suitable for clinical applications in cardiovascular diseases.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
Yong How Tan, Haylie R. Helms, Karina H. Nakayama
Summary: Cardiovascular disease and musculoskeletal conditions are major global health burdens with a lack of restorative treatment options. Regenerative medicine and tissue engineering offer potential solutions for repairing damaged tissues. Decellularized tissues and extracellular matrices play vital roles in regenerative biomaterials and their study can drive the development of novel regenerative therapies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Editorial Material
Dentistry, Oral Surgery & Medicine
H. Kagami
Article
Dentistry, Oral Surgery & Medicine
H. Kagami, M. Inoue, A. Kobayashi, A. Taguchi, X. Li, M. Yoshizawa
Article
Cell & Tissue Engineering
Xianqi Li, Ni Li, Kai Chen, Sakae Nagasawa, Michiko Yoshizawa, Hideaki Kagami
TISSUE ENGINEERING PART C-METHODS
(2018)
Article
Cell & Tissue Engineering
Kai Chen, Xianqi Li, Ni Li, Hongwei Dong, Yiming Zhang, Michiko Yoshizawa, Hideaki Kagami
STEM CELLS INTERNATIONAL
(2019)
Article
Cell & Tissue Engineering
Ni Li, Xianqi Li, Kai Chen, Hongwei Dong, Hideaki Kagami
STEM CELL RESEARCH & THERAPY
(2019)
Article
Cell & Tissue Engineering
Hideki Agata, Yoshinori Sumita, Tatsuro Hidaka, Mayumi Iwatake, Hideaki Kagami, Izumi Asahina
STEM CELLS INTERNATIONAL
(2019)
Article
Dentistry, Oral Surgery & Medicine
Eri Uchikawa, Michiko Yoshizawa, Xianqi Li, Nahomi Matsumura, Ni Li, Kai Chen, Hideaki Kagami
Summary: This study investigated a tissue engineering approach of using a scaffold or cells in tooth transplantation to accelerate bone formation and periodontal tissue regeneration. The results showed that the approach significantly increased bone volume and promoted periodontal tissue regeneration in a mouse model.
Article
Endocrinology & Metabolism
Lijuan Zhao, Shinichirou Ito, Atsushi Arai, Nobuyuki Udagawa, Kanji Horibe, Miroku Hara, Daisuke Nishida, Akihiro Hosoya, Rinya Masuko, Koji Okabe, Masashi Shin, Xianqi Li, Koichi Matsuo, Shinichi Abe, Satoru Matsunaga, Yasuhiro Kobayashi, Hideaki Kagami, Toshihide Mizoguchi
Summary: Severe dental tissue damage leads to odontoblast death followed by differentiation of dental pulp stem and progenitor cells into odontoblast-like cells for reparative dentin. The PTH1R cascade triggered by odontoblast death may serve as a therapeutic target for inducing dental regeneration. Increased expression of PTH1R enhances dentin formation in response to odontoblast depletion even without physical damage to dental tissue.
Article
Cell & Tissue Engineering
Yiming Zhang, Xianqi Li, Takahiro Chihara, Hongwei Dong, Hideaki Kagami
Summary: This study highlights the important role of IL-6 in modulating the efficacy of bone tissue engineering, showing both positive and negative effects on CBDCs. TNF-alpha, on the other hand, significantly suppresses the expression of osteogenic markers. In vivo experiments demonstrate a correlation between high IL-6 expression and new bone formation, while TNF-alpha expression does not show notable differences.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Cell & Tissue Engineering
Hongwei Dong, Xianqi Li, Kai Chen, Ni Li, Hideaki Kagami
Summary: The study found that using 5% dimethyl sulfoxide as a cryoprotectant can maximally preserve the viability, stemness, and osteogenic differentiation capability of spontaneous CB-MSC spheroids. These cryopreserved spheroids demonstrated superior new bone formation capability in vivo, and can be used for bone tissue engineering without osteogenic induction.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Medicine, Research & Experimental
Ni Li, Kai Chen, Hongwei Dong, Jing Yang, Michiko Yoshizawa, Hideaki Kagami, Xianqi Li
Summary: The study found that alliin inhibits adipocyte differentiation by reducing lipid accumulation and downregulating expression levels of multiple adipogenic differentiation-related genes. It also suppressed PKB/Akt and PI3K expression, indicating its potential therapeutic effect on metabolic diseases.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Takahiro Chihara, Yiming Zhang, Xianqi Li, Atsushi Shinohara, Hideaki Kagami
HISTOLOGY AND HISTOPATHOLOGY
(2020)
Article
Biology
Radyum Ikono, Agnia Vibriani, Indra Wibowo, Kurniawan Eko Saputro, Wibias Muliawan, Boy Muchlis Bachtiar, Etik Mardliyati, Endang Winiati Bachtiar, Nurul Taufiqu Rochman, Hideaki Kagami, Li Xianqi, Tokiko Nagamura-Inoue, Arinobu Tojo
BMC RESEARCH NOTES
(2019)
Article
Dentistry, Oral Surgery & Medicine
Xianqi Li, Feng Wu, Yiming Zhang, Jing Yang, Atsushi Shinohara, Hideaki Kagami
CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
(2016)