Review
Biochemistry & Molecular Biology
Pietro Gentile, Simone Garcovich
Summary: The skin acts as a natural barrier against UV radiation, but prolonged exposure to sunlight can lead to structural changes and photoaging. Adipose-derived mesenchymal stem cells and fat grafting have been utilized to combat signs of photoaging, such as wrinkles and pigmentation, through cellular activity and antioxidant effects.
Article
Agriculture, Dairy & Animal Science
Santina Di Bella, Vincenza Cannella, Francesco Mira, Patrizia Di Marco, Antonio Lastra, Francesca Gucciardi, Giuseppa Purpari, Annalisa Guercio
Summary: The study demonstrated that a high percentage of serum (80%) is necessary for optimal cryopreservation with 10% DMSO. Thawed cells at passages from P2 to P4, even after seven years, could still be considered for therapeutic application and in vitro study as they retain stem potential post-cryopreservation.
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Review
Cell Biology
Magdalena Kulus, Rafal Sibiak, Katarzyna Stefanska, Maciej Zdun, Maria Wieczorkiewicz, Hanna Piotrowska-Kempisty, Jedrzej M. Jaskowski, Dorota Bukowska, Kornel Ratajczak, Maciej Zabel, Paul Mozdziak, Bartosz Kempisty
Summary: Mesenchymal stem/stromal cells (MSCs) are extensively researched for regenerative medicine, with perinatal MSCs emerging as a valuable source devoid of ethical conflicts compared to embryonic stem cells, showing better self-renewal and differentiation properties. Understanding the anatomy of perinatal tissues and the signaling pathways involved in MSC differentiation is essential for their clinical applications.
Article
Biochemistry & Molecular Biology
Michiko Horiguchi, Yuko Okada, Yuya Turudome, Kentaro Ushijima
Summary: Type 1 diabetes mellitus leads to degeneration of organelles in adipose-derived stem cells, with the main characteristic being prominent enlarged spherical vesicles. The exosomes of T1DM ADSCs are found to be enlarged, reduced in number, and increased in the percentage positive for tetraspanin CD9, providing insight into the characteristics of stem cells in patients with type 1 diabetes mellitus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Liudmila Leppik, Anna Gempp, Zyrafete Kuci, Selim Kuci, Peter Bader, Halvard Boenig, Ingo Marzi, Dirk Henrich
Summary: The clinical breakthrough of bone tissue engineering relies on providing consistent pharmacological quality BTE products, which is hindered by the availability of stem cells. To overcome this, the suggestion is to immobilize heterologous osteoinductive MSCs onto osteoconductive matrices, freeze them, and then release them as ready-to-use products for surgery. In our study, we found that freeze-thawed hMSCs maintained their metabolic activity and showed favorable osteogenic potential, possibly through the activation of HIF1 alpha and YBX1 genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Summary: ADSCs tend to spontaneously differentiate into neuron-like cells during high confluence culture, with enhanced expression of neuronal markers and neurotrophins, while BMSCs do not show this tendency. Additionally, ADSCs do not exhibit spontaneous adipogenic and osteogenic differentiation during this process.
Article
Biochemistry & Molecular Biology
Hae-Ri Lee, Soo Kim, Sungho Shin, Seon-Yeong Jeong, Dae-Won Lee, Sun-Ung Lim, Ji Yeon Kang, Mi-Young Son, Cheolju Lee, Kyung-Rok Yu, Myungshin Kim, Il-Hoan Oh
Summary: In this study, the transition from primary MSCs to iMSCs was characterized by increased proliferative activity and variations in differentiation potential. iMSCs exhibited unique gene and protein expression patterns, resembling pericyte-like cells, and showed enhanced self-renewal and immune suppression capabilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Xuan-yu Jiang, Fang-wei Li, Yi-qing Chen, Jun-ren Fang, Sheng-kang Luo, Hai-bin Wang
Summary: This study found that using adipose-derived mesenchymal stem cell-derived exosomes (ADSC-Exos) can improve the short-term survival of cryopreserved fat grafts (within 4 weeks), but the overall improvement is limited in the long-term (after 8 weeks).
AESTHETIC PLASTIC SURGERY
(2023)
Article
Biotechnology & Applied Microbiology
Javad Harati, Xuelian Tao, Hosein Shahsavarani, Ping Du, Massimiliano Galluzzi, Kun Liu, Zhen Zhang, Peter Shaw, Mohammad Ali Shokrgozar, Haobo Pan, Peng-Yuan Wang
Summary: This study investigates the effect of polydopamine (PDA) coating on the epigenetic status and differentiation of human adipose-derived stem cells (hASCs). The results suggest that the PDA-Gel surface positively affects chondrogenic differentiation without impacting adipogenic and osteogenic differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Yicheng Qi, Wen Liu, Xiangsheng Wang, Nan Lu, Minglan Yang, Wei Liu, Jing Ma
Summary: The study evaluates the effects of adipose-derived MSCs from obese mice on body weight and glucose homeostasis, finding that these cells can reduce weight gain and improve obesity-related hyperglycemia in obese mice. This improvement is possibly attributed to increased lipolysis of inguinal fat and restoration of insulin receptor expression in muscle. Additionally, differential expression of CD90 and reduced levels of MCP-1 in O-ADSCs suggest that autologous ADSCs from obese individuals may be more effective for treating obesity and related hyperglycemia.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Rose Triantafillia Psaroudis, Urvashi Singh, Maximilien Lora, Peter Jeon, Abigail Boursiquot, Ursula Stochaj, David Langlais, Ines Colmegna
Summary: This study identifies DPP4 gene expression and CD26 protein abundance as markers of replicative senescence in MSC(AT). MSCs with high CD26 expression have reduced immunopotency, and senolytic treatment can decrease the frequency of CD26(high) MSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biochemistry & Molecular Biology
Masateru Nomura, Joseph George, Chieko Hashizume, Takashi Saito, Yoshimichi Ueda, Yasuhito Ishigaki, Mutsumi Tsuchishima, Mikihiro Tsutsumi
Summary: In this study, the effects of human adipose derived stem cells (hADSCs) on experimentally induced hepatic fibrosis and early cirrhosis in rats were evaluated. The results showed that treatment with hADSCs significantly improved liver functions and reduced hepatic fibrosis and early cirrhosis.
MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mengzhu Lv, Simeng Zhang, Bo Jiang, Sunrun Cao, Yuqing Dong, Liu Cao, Shu Guo
Summary: The study demonstrated that adipose-derived stem cell therapy can delay replicative senescence and shift metabolic homeostasis, ultimately delaying the aging process by promoting mitophagy and improving mitochondrial quality in cells. The research also showed that ADSC therapy can improve aging-related symptoms and phenotypes through mitochondrial quality control, offering significant potential for future applications in aging-related diseases.
Article
Orthopedics
Elke Maurer, Vera Wallmeier, Marie Reumann, Sabrina Ehnert, Christoph Ihle, Anna J. Schreiner, Ingo Flesch, Laura Emine Stollhof, Tina Histing, Andreas K. Nussler
Summary: The constant aging of the German population leads to increased susceptibility to infections and complications in hospitalised patients, especially in patients with surgical site infections (SSI). Limited studies have been conducted on the age distribution and mortality risk in patients with SSI, as well as the impact of underlying diseases and alcohol and nicotine consumption. This study found that age, pre-existing cardiac conditions, COPD, diabetes mellitus, and peripheral artery disease are significantly associated with an increased mortality risk in patients with SSI. The mortality risk increases exponentially between the ages of 50 and 60, with a 60-year-old patient with SSI having an equivalent mortality risk to an 80-year-old in the general population.
ZEITSCHRIFT FUR ORTHOPADIE UND UNFALLCHIRURGIE
(2023)
Article
Oncology
Gerda Schicht, Lena Seidemann, Rene Haensel, Daniel Seehofer, Georg Damm
Summary: Energy metabolism plays a central role in liver cancer, and metabolic alterations are crucial for diagnostic screening and therapeutic intervention. This study compared the metabolic profiles of commonly used hepatoma cell lines (HCLs) and primary human hepatoma cells (PHCs) to investigate their representation of hepatocellular carcinoma (HCC) cells. The findings showed that HCLs are a poor substitute for primary cells, and metabolic adaptions had already occurred in non-tumor-bearing liver tissue of HCC patients. These results have implications for optimizing HCC models for future research.
Article
Biochemistry & Molecular Biology
Caren Linnemann, Andreas K. Nussler, Tina Histing, Sabrina Ehnert
Summary: This study found that NETs severely impaired the viability and migration of SCP-1 cells, and had a persistent negative effect on osteogenic differentiation. The addition of DNase or protease inhibitors failed to reverse the negative effect of NETs, while a short febrile-range temperature treatment successfully reduced the toxicity and membrane destruction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Huizhi Guo, Weidong Weng, Shuncong Zhang, Helen Rinderknecht, Bianca Braun, Regina Breinbauer, Purva Gupta, Ashok Kumar, Sabrina Ehnert, Tina Histing, Andreas K. Nussler, Romina H. Aspera-Werz
Summary: Maqui berry extract and ginseng extract can reduce bone cell damage caused by cigarette smoke and restore their function. These two natural antioxidants protect against CSE through the inhibition of the NF-kappa B signaling pathway and activation of the Nrf2 signaling pathway.
Article
Biotechnology & Applied Microbiology
Michael Ronniger, Blanche Aguida, Christina Stacke, Yangmengfan Chen, Sabrina Ehnert, Niklas Erdmann, Georg Eschenburg, Karsten Falldorf, Marootpong Pooam, Anthony Wing, Margaret Ahmad
Summary: The article presents a technically simple and reproducible EMF exposure protocol that can be used in any lab to study the effects of extremely low-frequency electromagnetic fields on living systems. By controlling the experiment parameters, researchers were able to accurately determine the effect of changing single ELF-MF parameters on biological responses. This research has the potential to advance the understanding and application of ELF-MF/PEMF research in the scientific community.
BIOENGINEERING-BASEL
(2022)
Article
Geriatrics & Gerontology
Tanja Krauss, Simone Heisz, Julius Honecker, Olga Prokopchuk, Marc Martignoni, Klaus-Peter Janssen, Melina Claussnitzer, Hans Hauner, Claudine Seeliger
Summary: This study aimed to identify specific miRNAs related to organ-specific cancer cachexia (CCx) and explore their functional role in humans. The findings showed that miR-122-5p, miR-27b-3p, miR-375, and miR-424-5p were associated with CCx and may contribute to tissue wasting and skeletal muscle atrophy through the regulation of catabolic signals.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Medicine, General & Internal
Artur Rebelo, Jumber Partsakhashvili, Ulrich Ronellenfitsch, Endres John, Joerg Kleeff, Joerg Ukkat
Summary: This study analyzed the outcome of emergency thoracic endovascular aortic repair (TEVAR) in the treatment of primary and secondary aortic pathologies. The results showed that in-hospital mortality and morbidity were still considerable after emergency TEVAR. Pre- and postoperative levels of hemoglobin, creatinine, and lactate may be valuable in predicting patient outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Health Care Sciences & Services
Yoshiaki Sunami, Ulrich Ronellenfitsch, Jorg Kleeff, Artur Rebelo
Summary: This study aims to evaluate the impact of FAP expression on survival and clinical characteristics in gastrointestinal tumors. A systematic review and meta-analysis will be conducted to compare patients with and without FAP overexpression in terms of postoperative survival, histological differentiation, tumor invasion, lymph node metastases, and distant metastases. The expected results will provide new evidence on the prognostic value of FAP in gastrointestinal tumors.
JMIR RESEARCH PROTOCOLS
(2023)
Article
Biotechnology & Applied Microbiology
Maximilian M. Menger, Ruben Manuschewski, Sabrina Ehnert, Mika F. Rollmann, Tanja C. Maisenbacher, Anne L. Tobias, Michael D. Menger, Matthias W. Laschke, Tina Histing
Summary: With a growing elderly population, trauma and reconstructive surgery face challenges in treating geriatric patients. The impact of aging on bone healing remains controversial, prompting an investigation on fracture healing in young adult and aged CD-1 mice. The study revealed delayed callus remodeling and impaired bending stiffness in aged animals, suggesting delayed fracture healing. However, the overall healing capacity of fractured femora was not affected by aging.
BIOENGINEERING-BASEL
(2023)
Editorial Material
Toxicology
Klaus-Michael Wollin, Monika Batke, Georg Damm, Alexius Freyberger, Ursula Gundert-Remy, Aswin Mangerich, Jan G. Hengstler, Falko Partosch, Thomas Schupp, Anna Sonnenburg, Heidi Foth
Summary: PFASs are substances that contain fully fluorinated methyl or methylene carbon atoms. They have excellent technical properties and are used in various applications. There are over 10,000 compounds in this group, but data on their toxicokinetics and effects is lacking for most of them. The high persistence of PFASs and their degradation products poses a problem, and the European Commission plans to take action. However, closing the data gaps would take a long time, and the precautionary principle has been chosen as a suitable approach. Communication about PFASs has led to misunderstandings, and it should clarify the justification for the broad inclusion of substances based on the precautionary principle, communicate data gaps and current knowledge, mention the possibility of derogation for essential use, discuss suitable substitutes to avoid unintended health consequences, and emphasize the need for innovation in remediation techniques to address persistent environmental contamination.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Caren Linnemann, Filiz Sahin, Yangmengfan Chen, Karsten Falldorf, Michael Ronniger, Tina Histing, Andreas K. Nussler, Sabrina Ehnert
Summary: This study aimed to evaluate the effect of a 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) on neutrophil extracellular trap (NET) formation. The study found that ELF-PEMF exposure did not induce reactive oxygen species and Ca2+-influx in neutrophils, and it reduced the amount of NET release. Therefore, ELF-PEMF exposure can be started immediately after fracture treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Artur Rebelo, Joerg Kleeff, Yoshiaki Sunami
Summary: This review provides an in-depth understanding of metabolic reprogramming and the mevalonate pathway in pancreatic cancer. It emphasizes the role of cholesterol metabolism in cancer progression and explores potential therapeutic approaches targeting these processes. The review also highlights promising pharmaceutical candidates for pancreatic cancer therapy.
Article
Cell Biology
Sabrina Ehnert, Helen Rinderknecht, Chao Liu, Melanie Voss, Franziska M. Konrad, Wiebke Eisler, Dorothea Alexander, Kristian-Christos Ngamsri, Tina Histing, Mika F. Rollmann, Andreas K. Nussler
Summary: Chronic wounds have a significant impact on individuals, healthcare systems, and societies worldwide. This study aimed to characterize the differences between acute and chronic wounds regarding angiogenesis, inflammation, and extracellular matrix (ECM) formation and degradation. The results suggest that chronic wounds show increased levels of MMP9 and absence of TGF-beta target gene regulation, indicating the presence of a TGF-beta inhibitor. Increased expression of BAMBI suggests its potential regulatory role in the development of chronic wounds.
Article
Food Science & Technology
Helen Rinderknecht, Alana Mayer, Tina Histing, Sabrina Ehnert, Andreas Nuessler
Summary: Smoking has a significant negative impact on early fracture healing, including inflammation, osteogenesis, and angiogenesis. However, the use of Panax ginseng and maqui berry extract did not have a noticeable positive effect on the early stages of fracture repair in smokers.
Article
Biology
Caren Linnemann, Filiz Sahin, Ningna Li, Stefan Pscherer, Friedrich Goetz, Tina Histing, Andreas K. Nussler, Sabrina Ehnert
Summary: Diabetes is a worldwide disease that often leads to delayed or impaired wound healing. This study investigated the effect of insulin on neutrophil extracellular trap (NET) formation, which is associated with impaired wound healing in diabetic patients. Insulin was found to delay NET formation induced by certain stimuli, but had no effect on another stimulus. Mechanistically, insulin induced reactive oxygen species and phosphorylation of certain proteins, leading to bacterial killing. Insulin may be a potential tool for regulating NET formation in diabetic wound healing.