Article
Engineering, Biomedical
Xiang Zeng, Qing-shuai Wei, Ji-chao Ye, Jun-hua Rao, Mei-guang Zheng, Yuan-huan Ma, Li-zhi Peng, Ying Ding, Bi-qin Lai, Ge Li, Shi-xiang Cheng, Eng-Ang Ling, Inbo Han, Yuan-shan Zeng
Summary: This study assessed the biosafety and efficacy of a three-dimensional gelatin sponge (3D-GS) scaffold in a non-human primate spinal cord injury model. The results demonstrated that the scaffold had good biocompatibility and significant effectiveness in the structural repair of injured spinal cord tissue, making it suitable for the treatment of patients with spinal cord injury.
Article
Engineering, Environmental
Haifeng Liu, Xiaojun Yan, Jingwei Jiu, Jiao Jiao Li, Yuanyuan Zhang, Guishan Wang, Dijun Li, Lei Yan, Yanan Du, Bin Zhao, Bin Wang
Summary: Current approaches for treating spinal cord injury (SCI) mainly involve cell transplantation, but the complex microenvironment created by SCI reduces cell activity and leads to poor therapeutic outcomes. Researchers have developed functional tissue constructs using self-assembled mesenchymal stem cell (MSC) microtissues, which show improved cellular activity and increased secretion of nerve regeneration-related factors. These microtissues have shown therapeutic effects in a rat model of complete spinal cord transection, potentially through mechanisms related to the regulation of response mediator protein 2 (CRMP2) phosphorylation and inhibition of the inflammatory response.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Cell & Tissue Engineering
Mian Wu, Lifeng Chen, Yuhan Qi, Hai Ci, Shan Mou, Jie Yang, Qiaoyu Yuan, Weiqi Yao, Zhenxing Wang, Jiaming Sun
Summary: Using umbilical cord mesenchymal stem cells (hUCMSCs) as seed cells for tissue-engineered breast construction has shown significant advantages, promoting adipose tissue regeneration and enhancing angiogenesis. The transplantation of hUCMSCs was found to increase fat tissue content, reduce macrophage numbers, and exhibit good differentiation capability in adipose tissue.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Zixian Wang, Xiuxiu Zhang, Limin Xue, Gangwei Wang, Xinda Li, Jianwei Chen, Ruxiang Xu, Tao Xu
Summary: A co-flow microfluidics-based system was developed to generate uniform and size-controllable gelatin-based microcarriers (GMs) for mesenchymal stromal cells (MSCs) expansion and tissue engineering. GMs exhibited superior homogeneity, efficiency of cellular attachment, expansion, and harvest, and MSCs expanded on GMs showed high viability and multipotency. MSC-loaded GMs were also printable and could serve as building blocks for tissue regeneration scaffolds.
REGENERATIVE BIOMATERIALS
(2023)
Article
Engineering, Environmental
Jian Cao, Jiahe Wu, Jiafu Mu, Lingmin Lin, Xunqi Zhang, Tianchen Huang, Teng Ma, Manning Zhu, Xiaoyang Dai, Xuhua Wang, Shiqing Feng, Jian-Qing Gao
Summary: Implanting MSCs in SCI repair holds promise, but the harsh environment and excessive ROS threaten MSC survival. The dual-phase pathology of acute and chronic phases makes it challenging to alleviate severe inflammation and promote functional recovery. The RF-pGel-MS scaffold system efficiently scavenges ROS and boosts stem cell therapeutics, showing significant efficacy in locomotor and electro-physiology recovery in SCI rats.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Alexandra Roi, Ciprian Roi, Meda Lavinia Negrutiu, Laura Cristina Rusu, Mircea Rivis
Summary: Dental stem cells, such as human periapical cyst mesenchymal stem cells (hPCy-MSCs), derived from the oral cavity, possess multilineage differentiation potential, immunomodulatory activity, and repair capacity. These cells exhibit similar characteristics to mesenchymal stem cells (MSCs) and hold great significance for the development of clinical applications in dentistry.
Article
Biotechnology & Applied Microbiology
Jiyun Cheng, Zheng Chen, Can Liu, Mei Zhong, Shihuan Wang, Yongjian Sun, Huiquan Wen, Tao Shu
Summary: This study successfully utilized 3D gelatin methacrylate hydrogel as a delivery system for transplanted exosomes, enhancing neuronal differentiation and extension of neural stem cells, while promoting neurogenesis and attenuating glial scars in damaged lesions.
Article
Engineering, Biomedical
Caroline D. Hoemann, Javier Rodriguez Gonzalez, Jessica Guzman-Morales, Gaoping Chen, Ebrahim Jalali Dil, Basil D. Favis
Summary: This study found that non-inflammatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold, and were compatible with trabecular bone integration. Compared to drill-only defects, the 83-99-PCL scaffold induced angiogenesis and slight bone resorption, with the smallest change in bone volume fraction.
BIOACTIVE MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Ravena P. do Nascimento, Livia B. de Jesus, Markley S. Oliveira-Junior, Aurea M. Almeida, Eduardo L. T. Moreira, Bruno D. Paredes, Jorge M. David, Bruno S. F. Souza, Maria de Fatima D. Costa, Arthur M. Butt, Victor Diogenes A. Silva, Silvia L. Costa
Summary: The study found that Agathisflavone can protect injured spinal cord tissue and exhibit therapeutic effects in a rat model of acute spinal cord injury. It also promotes neurogenesis and increases the expression of neurotrophins while reducing inflammation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Physical
Markus Merk, Orlando Chirikian, Christian Adlhart
Summary: Recent advancements have led to the creation of highly porous three-dimensional nanofiber scaffolds using electrospinning and self-assembly techniques for various tissue engineering applications. These scaffolds, made of biologically relevant materials, exhibit excellent mechanical properties and cell cytocompatibility, showing great potential for future tissue engineering applications.
Review
Nanoscience & Nanotechnology
Christy Kwokdinata, Vaibavi Ramanujam, Jiahui Chen, Paula Nunes de Oliveira, Mui Hoon Nai, Wai Hon Chooi, Chwee Teck Lim, Shi Yan Ng, Laurent David, Sing Yian Chew
Summary: This study found that using injectable hyaluronan-gelatin hydrogel in transplantation therapy can promote cell survival and neural differentiation of human spinal cord progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Serena Barachini, Marina Montali, Francesca M. Panvini, Vittoria Carnicelli, Gian Luca Gatti, Nicola Piolanti, Enrico Bonicoli, Michelangelo Scaglione, Gabriele Buda, Paolo D. Parchi
Summary: Mesangiogenic progenitor cells (MPCs) isolated from human bone marrow mononuclear cells have the ability to differentiate into mesenchymal stromal cells while retaining endothelial differentiative potential. Their power to couple mesengenesis and angiogenesis highlights their tissue regenerative potential, particularly in musculoskeletal tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Narmadaa Raman, Siti A. M. Imran, Khairul Bariah Ahmad Amin Noordin, Wan Safwani Wan Kamarul Zaman, Fazlina Nordin
Summary: This review highlights the process of mechanotransduction, the effects of mechanical forces on cells and tissues, and the regulation of mesenchymal stem cell differentiation by mechanical factors. Research has shown that mechanical forces play a crucial role in cell sensing and adaptation to the environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Yuan-huan Ma, Hui-juan Shi, Qing-shuai Wei, Qing-wen Deng, Jia-hui Sun, Zhou Liu, Bi-qin Lai, Ge Li, Ying Ding, Wan-ting Niu, Yuan-shan Zeng, Xiang Zeng
Summary: The study introduces a mechanically enhanced decellularized spinal cord (DSC) scaffold with a thin poly (lactic-co-glycolic acid) (PLGA) outer shell, showing promise for effective in situ neuroengineering after spinal cord injury (SCI).
Article
Engineering, Biomedical
Bi-Qin Lai, Xiang Zeng, Wei-Tao Han, Ming-Tian Che, Ying Ding, Ge Li, Yuan-Shan Zeng
Summary: For millennia, adult mammals have been unable to recover function lost after severe spinal cord injury due to a failure of brain-derived nerve fiber regeneration across the lesion. Various potential approaches rely on neuronal relays to reconnect the segregated neural networks of the spinal cord, including the use of stem cell-derived neuronal relays and functional electrical stimulation.
Review
Neurosciences
Yuan-Shan Zeng, Ying Ding, Hao-Yu Xu, Xiang Zeng, Bi-Qin Lai, Ge Li, Yuan-Huan Ma
Summary: The incidence and disability rate of spinal cord injury (SCI) are high worldwide. Traditional Chinese medicine technique Governor Vessel electro-acupuncture (GV-EA) has been found to improve the microenvironment of injured spinal cord, and adult stem cell transplantation has potential for reconnecting severed spinal cord tissue and restoring motor and sensory functions. However, no single treatment approach has proven satisfactory, and combining different treatments may be more promising.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Engineering, Biomedical
Ge Li, Bao Zhang, Jia-hui Sun, Li-yang Shi, Meng-yao Huang, Li-jun Huang, Zi-jing Lin, Qiong-yu Lin, Bi-qin Lai, Yuan-huan Ma, Bin Jiang, Ying Ding, Hong-bo Zhang, Miao-xin Li, Ping Zhu, Ya-qiong Wang, Xiang Zeng, Yuan-shan Zeng
Summary: By engineering TrkC-modified neural network tissue in a 3D scaffold, tissue repair for spinal cord injury was improved, leading to enhanced motor function recovery in rats.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Bi-Qin Lai, Yu-Rong Bai, Wei-Tao Han, Bao Zhang, Shu Liu, Jia-Hui Sun, Jia-Lin Liu, Ge Li, Xiang Zeng, Ying Ding, Yuan-Huan Ma, Ling Zhang, Zheng-Hong Chen, Jun Wang, Yuan Xiong, Jin-Hua Wu, Qi Quan, Ling-Yan Xing, Hong-Bo Zhang, Yuan-Shan Zeng
Summary: This study proposes a strategy for constructing niche-specific spinal white matter-like tissue using decellularized optic nerve, which shows significant improvement in motor functions in a rat model with a white matter defect. The study provides a potential solution to the directional axon regeneration and remyelination barriers during spinal cord injury repair.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Meng-yun Cai, Chung-Yin Yip, Kewu Pan, Yan Zhang, Renee Wan-Yi Chan, Wood Yee Chan, Wing-Hung Ko
Summary: This study reveals the pivotal role of carbon monoxide (CO) in regulating oxidative stress-induced epithelial senescence through the SIRT3/MnSOD/p53/p21 pathway. These findings have important implications for the prevention and treatment of age-associated respiratory pathologies.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Clinical Neurology
Yuan-huan Ma, Qing-yue Liang, Ying Ding, Inbo Han, Xiang Zeng
Summary: Spinal cord injury (SCI) currently lacks effective treatment, but mesenchymal stem cell (MSC) therapy has shown promise in both experimental and clinical studies. The exact mechanism of how MSCs contribute to spinal cord repair is yet to be fully understood. This review summarizes the current research findings on the role of MSCs in improving complex pathology after SCI, highlighting the multimodal repair mechanism of MSCs targeting multiple events in the secondary injury cascade. Recent results also suggest the differentiation of transplanted MSCs into perineurium-like cells in the injured spinal cord. These findings support the clinical use of MSCs in SCI patients and hold potential for future cell-based therapies.
Article
Engineering, Biomedical
Bi-Qin Lai, Rong-Jie Wu, Wei-Tao Han, Yu-Rong Bai, Jia-Lin Liu, Hai-Yang Yu, Shang-Bin Yang, Lai-Jian Wang, Jia-Le Ren, Ying Ding, Ge Li, Xiang Zeng, Yuan-Huan Ma, Qi Quan, Ling-Yan Xing, Bin Jiang, Ya-Qiong Wang, Ling Zhang, Zheng-Hong Chen, Hong-Bo Zhang, Yuan-Feng Chen, Qiu-Jian Zheng, Yuan-Shan Zeng
Summary: In this study, a rat model of spinal cord injury was used to evaluate the effects of neural stem cell-derived spinal cord-like tissue (SCLT) transplantation and tail nerve electrical stimulation (TNES) on nerve conduction recovery and voluntary movement in rats. TNES promoted axon regeneration and remyelination, improved neural transmission efficiency, and enhanced muscle function and metabolism. The combination of SCLT and TNES could potentially offer a breakthrough in restoring voluntary movement and muscle control for spinal cord injury patients.
Article
Immunology
Jia-Lin Liu, Shuai Wang, Zheng-Hong Chen, Rong-Jie Wu, Hai-Yang Yu, Shang-Bin Yang, Jing Xu, Yi-Nan Guo, Ying Ding, Ge Li, Xiang Zeng, Yuan-Huan Ma, Yu-Lai Gong, Chuang-Ran Wu, Li-Xin Zhang, Yuan-Shan Zeng, Bi-Qin Lai
Summary: Transcranial intermittent theta-burst stimulation (iTBS) can reduce inflammation and neuronal apoptosis, promote axonal regeneration and synaptic plasticity, and improve motor function recovery and corticospinal tract regeneration after spinal cord transection injury.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Junhua Wang, Qingshuai Wei, Yue Yang, Mingtian Che, Yuanhuan Ma, Lizhi Peng, Haiyang Yu, Huijuan Shi, Guanheng He, Rongjie Wu, Ting Zeng, Xiang Zeng, Wenbin Ma
Summary: This study utilized a novel porous scaffold-based three dimensional culture technique to obtain 4D culture-derived Small Extracellular Vesicles (sEVs) from human umbilical cord mesenchymal stem cells (hUC-MSCs). The 4D-sEVs exhibited altered protein profiles compared to those derived from 2D culture conditions, with significant upregulation of Epidermal Growth Factors Receptor (EGFR) and Insulin-like Growth Factor Binding Protein 2 (IGFBP2). The endocytosis of 4D-sEVs induced macrophages/microglia polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotype and reduced neuroinflammation, leading to significant neuroprotection and increased tissue repair in spinal cord injury (SCI).
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Xiang Zeng, Qing-shuai Wei, Ji-chao Ye, Jun-hua Rao, Mei-guang Zheng, Yuan-huan Ma, Li-zhi Peng, Ying Ding, Bi-qin Lai, Ge Li, Shi-xiang Cheng, Eng-Ang Ling, Inbo Han, Yuan-shan Zeng
Summary: This study assessed the biosafety and efficacy of a three-dimensional gelatin sponge (3D-GS) scaffold in a non-human primate spinal cord injury model. The results demonstrated that the scaffold had good biocompatibility and significant effectiveness in the structural repair of injured spinal cord tissue, making it suitable for the treatment of patients with spinal cord injury.
Article
Cell Biology
Yu-Rong Bai, Bi-Qin Lai, Wei-Tao Han, Jia-Hui Sun, Ge Li, Ying Ding, Xiang Zeng, Yuan-Huan Ma, Yuan-Shan Zeng
Summary: The porcine decellularized optic nerve loaded with neurotrophin-3-overexpressing Schwann cells can promote the directional growth of regenerating spinal cord axons and myelin regeneration.
NEURAL REGENERATION RESEARCH
(2021)
