Article
Oncology
Caique Almeida Machado Costa, Xian-Feng Wang, Calder Ellsworth, Wu-Min Deng
Summary: Polyploidy, a cell status with more than two sets of genomic DNA, is a conserved strategy across species that contributes to cell size, biosynthetic production, and developmental processes in Drosophila; however, polyploid cells are also prevalent in Drosophila tumors, playing significant roles in tumor growth and invasiveness.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biology
Xiaowen Ji, Jiajia Gao, Tian Wei, Li Jin, Guiran Xiao
Summary: This study reveals that the zinc transporter FOI is essential for fat body endoreplication and larval growth in Drosophila. Knockdown of FOI results in failure of fat body cells to reach normal size, decreased fat body size, and pupal lethality. The intracellular zinc depletion caused by FOI knockdown leads to oxidative stress, activation of the ROS-JNK signaling pathway, and inhibition of Myc expression, which is required for tissue endoreplication and larval growth in Drosophila.
Editorial Material
Biochemistry & Molecular Biology
Divya R. Iyer, Alan D. D'Andrea
Summary: This study reveals that eliminating FANCD2 from stalled forks via FBXL12-mediated degradation enables cells to tolerate oncogene-induced replication stress, suggesting that FBXL12 could be a promising target for cancer treatment.
Article
Cell Biology
Xian-Feng Wang, Sheng-An Yang, Shangyu Gong, Chih-Hsuan Chang, Juan Martin Portilla, Deeptiman Chatterjee, Jerome Irianto, Hongcun Bao, Yi-Chun Huang, Wu-Min Deng
Summary: This study shows that upregulation of the Notch signaling pathway induces polyploid mitosis in tumor cells in a Drosophila solid-tumor model, and DNA-damage response genes are upregulated in these tumors and required for ploidy-reduction division. These findings suggest that polyploidy and associated cell-cycle variants play a critical role in increasing tumor-cell heterogeneity and genome instability during cancer progression.
DEVELOPMENTAL CELL
(2021)
Article
Genetics & Heredity
Minhee Kim, Keemo Delos Santos, Nam-Sung Moon
Summary: Polyploidy is a crucial process in development, associated with cellular stress, aging, and pathological conditions. The endocycle, which involves repeated G and S phases without mitosis, is regulated by E2F and plays a key role in producing polyploid cells. In Drosophila, an alternative isoform of de2f1, dE2F1b, is shown to control the expression of the Cdk inhibitor Dap and is required for proper CycE-Cdk2 activity during the endocycle. Additionally, dE2F1b is involved in establishing a negative feedback loop in S phase through regulating PCNA expression.
Article
Plant Sciences
Alexandra Martin-Esquinas, Lourdes Hernandez-Apaolaza
Summary: The study suggests that Si in rice not only acts as an apoplastic barrier but also protects chloroplasts, alters endoreplication cycle, providing a memory effect to cope with different stresses, which is beneficial for plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Developmental Biology
Dmitrij Dedukh, Marie Altmanova, Jiri Klima, Lukas Kratochvil
Summary: Obligate parthenogenesis has evolved multiple times in reptiles through interspecific hybridization, resulting in the formation of diploid and triploid lineages. This study shows that oocytes in several widespread parthenogenetic complexes of geckos undergo meiosis and produce unreduced eggs through premeiotic endoreplication, allowing for the emergence of fertile polyploid lineages within parthenogenetic complexes.
Article
Biochemistry & Molecular Biology
Kodai Hara, Asami Hishiki, Takako Hoshino, Kiho Nagata, Nao Iida, Yukimasa Sawada, Eiji Ohashi, Hiroshi Hashimoto
Summary: The RAD9-RAD1-HUS1 complex (9-1-1) plays a crucial role in DNA damage checkpoints in eukaryotes. Loading of 9-1-1 onto recessed DNA is facilitated by the RAD17 RFC-like complex (RAD17-RLC) and is necessary for activation of the ATR-CHK1 checkpoint pathway. The interaction between 9-1-1 and the protein RHINO, which contains a RAD1-binding motif, is shown to disturb the interaction between RAD17 and 9-1-1. These findings provide a deeper understanding of how RAD17-RLC recognizes 9-1-1 and suggest a functional role for RHINO in 9-1-1 loading/unloading and checkpoint activation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dmitrij Dedukh, Anatolie Marta, Karel Janko
Summary: The transition from sexual reproduction to asexuality in hybrid animals is often triggered by genome endoreplication, with most gonocytes unable to proceed beyond pachytene due to defects in bivalent formation. The rate of endoreplication differs between hybrids from natural clones and experimentally produced hybrids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Eunjin Ryu, Na Young Ha, Woojae Jung, Juyeong Yoo, Kyungjae Myung, Sukhyun Kang
Summary: This study identified several motifs in the ATAD5 ULD that play crucial roles in the PCNA-unloading process. The C-terminus of ULD is required for the stable association of RFC2-5 to form active RLC, while the N-terminus of ULD participates in the opening of the PCNA ring. ATAD5-RLC shows stronger binding to open-liable PCNA compared to the wild type.
Review
Oncology
Yanwei Song, Yucui Zhao, Zheng Deng, Ruyi Zhao, Qian Huang
Summary: Polyploidy is a conserved mechanism in cell development and stress responses, and can be induced by various stress treatments, leading to the formation of giant tumor cells. These cells may escape death and contribute to tumor recurrence and metastasis, providing potential targets for cancer therapy. However, differences in formation mechanisms between these cells and other polyploid cancer cells remain unclear.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Izabela Kolodziejczyk, Przemyslaw Tomczyk, Andrzej Kazmierczak
Summary: In recent years, new insights into a cell replication process known as endoreplication, which is common in plants and accompanies changes in animal development, have emerged. This paper aims to shed light on this phenomenon and promote understanding of its potential applications in plant cultivation. Endoreplication differs from the classical somatic cell cycle and is a compensation for the relatively small amount of genetic material in plants. It allows for the multiplication and active use of genetic material, resulting in desirable features such as vibrant colors, aroma, taste, nutrient accumulation, stress resistance, tolerance to adverse conditions, and faster organ growth in plants. The article also discusses practical applications and limitations of endoreplication.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Hunter C. Herriage, Yi-Ting Huang, Brian R. Calvi
Summary: Apoptosis prevents the inappropriate acquisition of extra copies of the genome, known as polyploidy, but the polyploid state can suppress apoptosis. The mechanisms linking apoptosis and polyploid cell cycles are still largely unknown, and studying the regulation of apoptosis in development and cancer could lead to more effective therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Article
Microbiology
Lucrecia S. Iriarte, Cristian I. Martinez, Natalia de Miguel, Veronica M. Coceres
Summary: This study shows that Tritrichomonas foetus and Trichomonas vaginalis can divide by multiple fission under stress conditions, which provides valuable data about the cell division process of these parasites.
MICROBIOLOGY SPECTRUM
(2023)
Article
Reproductive Biology
Tomas Tichopad, Roman Franek, Marie Dolezalkova-Kastankova, Dmitrij Dedukh, Anatolie Marta, Karel Halacka, Christoph Steinbach, Karel Janko, Martin Psenicka
Summary: Interspecific hybridization may lead to a transition from sexual reproduction to asexual reproduction, but the reasons for this change are not well understood. In this study, we investigated the triggers of asexual reproduction and found that it is directly influenced by the hybrid genetic constitution of the cell. Additionally, the execution of asexual development is regulated by signals from the surrounding gonadal environment and somatic factors.
BIOLOGY OF REPRODUCTION
(2022)
Article
Oncology
Ibuki Ueoka, Akari Takai, Mizuki Yamaguchi, Tomohiro Chiyonobu, Hideki Yoshida, Masamitsu Yamaguchi
EXPERIMENTAL CELL RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Steven J. Marygold, Helen Attrill, Elena Speretta, Kate Warner, Michele Magrane, Maria Berloco, Sue Cotterill, Mitch McVey, Yikang Rong, Masamitsu Yamaguchi
Article
Biochemistry & Molecular Biology
Hang Thi Nguyet Pham, Hong Nguyen Tran, Phuong Thi Nguyen, Xoan Thi Le, Khoi Minh Nguyen, Sinh Viet Phan, Masanori Yoneyama, Kiyokazu Ogita, Taro Yamaguchi, William R. Folk, Masamitsu Yamaguchi, Kinzo Matsumoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Salinee Jantrapirom, Yosuke Enomoto, Jirarat Karinchai, Mizuki Yamaguchi, Hideki Yoshida, Eiichiro Fukusaki, Shuichi Shimma, Masamitsu Yamaguchi
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Masamitsu Yamaguchi, Kentaro Omori, Satoshi Asada, Hideki Yoshida
Summary: ALS and CMT are common neurodegenerative disorders with significant phenotypic heterogeneity, potentially due to modifiers that influence disease characteristics. Epigenetic regulation of biological functions through gene expression may be an important mechanism in these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Eric Hyrmeya Savadogo, Yui Shiomi, Junko Yasuda, Toshiharu Akino, Masamitsu Yamaguchi, Hideki Yoshida, Takanari Umegawachi, Ryo Tanaka, Dang Ngoc Anh Suong, Kenji Miura, Kazufumi Yazaki, Sakihito Kitajima
Summary: Genes of the PLAT protein family play a role in plant defense against insects through the involvement of anti-microbe or anti-insect proteins in laticifer cells. These genes induce an indirect insect resistance response in the host plant, as well as possibly causing direct cytotoxic effects on insects. Larval growth of insects is significantly affected by the expression of PLAT proteins from different plant species, highlighting the conserved anti-insect activity of the PLAT protein family in the plant kingdom.
Article
Biochemistry & Molecular Biology
Hang Thi Nguyet Pham, Hong Nguyen Tran, Xoan Thi Le, Ha Thi Do, Tue Trong Nguyen, Chien Le Nguyen, Hideki Yoshida, Masamistu Yamaguchi, Folk R. William, Kinzo Matsumoto
Summary: This study found that a chemically characterized extract from Ilex kudingcha can improve behavioral traits in a Drosophila melanogaster rugose mutant, resembling human Autism Spectrum Disorder (ASD) traits. The extract also increased synaptic bouton number in Drosophila rugose and altered gene expression in the mouse hippocampus. These results suggest the potential of Ilex kudingcha extract as a treatment for some forms of ASD.
NEUROCHEMICAL RESEARCH
(2021)
Article
Neurosciences
Yuuka Muraoka, Atsushi Nikaido, Ryosuke Kowada, Hiroshi Kimura, Masamitsu Yamaguchi, Hideki Yoshida
Summary: Mutations in the FIG 4 gene are associated with various disorders, possibly due to dysregulated epigenetics. HDAC1 has been identified as an important epigenetic regulator of FIG 4, suggesting potential therapeutic targets for FIG 4-related disorders such as CMT.
Correction
Oncology
Hisanori Matsubara, Reiji Tanaka, Tatsuya Tateishi, Hideki Yoshida, Masamitsu Yamaguchi, Takao Kataoka
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Oncology
Masamitsu Yamaguchi, Im-soon Lee, Salinee Jantrapirom, Kojiro Suda, Hideki Yoshida
Summary: Drosophila is being used as a model for studying human diseases, with functional homologues of a large percentage of human disease-related genes found in Drosophila. This review discusses Drosophila models targeting genes associated with ALS and CMT, highlighting potential therapeutic targets and diagnostic markers.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Oncology
Ngoc-Lan Thi Nguyen, Ngoc-Dzung Thi Dang, Quang-Huy Dang, Van-Chuc Tran, Hoang-Long Vo, Masamitsu Yamaguchi, Thanh-Van Ta
Summary: The study found that the polymorphisms of Mucin 1 gene, specifically rs4072037 and rs2070803, are associated with gastric cancer risk, with the AA genotype potentially increasing risk while the AG genotype may lower it. Combining factors such as age, gender, alcoholism, and personal history of gastric disease significantly elevate the risk of gastric cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Ryosuke Kowada, Atsushi Kodani, Hiroyuki Ida, Masamitsu Yamaguchi, Im-Soon Lee, Yasushi Okada, Hideki Yoshida
Summary: The homolog of human SCO2, Scox, plays an essential role in mitochondrial function and locomotive behavior in Drosophila. Knockdown of Scox in glial cells impairs mitochondrial morphology and function, as well as locomotive behavior, mimicking the pathological phenotypes observed in humans with mutations in the SCO2 gene.
SCIENTIFIC REPORTS
(2021)
Correction
Multidisciplinary Sciences
Tomoki Hirashima, Ryo Tanaka, Masamitsu Yamaguchi, Hideki Yoshida
SCIENTIFIC REPORTS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Masamitsu Yamaguchi, Shinya Yamamoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Yu Hiramatsu, Yuji Okamoto, Akiko Yoshimura, Jun-Hui Yuan, Masahiro Ando, Yujiro Higuchi, Akihiro Hashiguchi, Eiji Matsuura, Fumihito Nozaki, Tomohiro Kumada, Kei Murayama, Mikiya Suzuki, Yuki Yamamoto, Naoko Matsui, Yoshimichi Miyazaki, Masamitsu Yamaguchi, Youji Suzuki, Jun Mitsui, Hiroyuki Ishiura, Masaki Tanaka, Shinichi Morishita, Ichizo Nishino, Shoji Tsuji, Hiroshi Takashima
Summary: This study identified causative mutations in mitochondrial-related nuclear genes in suspected hereditary peripheral neuropathy patients. Whole-exome sequencing was performed on a large cohort of Japanese patients, and novel pathogenic variants were detected in four mitochondrial-related nuclear genes. These patients exhibited sensory and motor axonal polyneuropathy, along with central nervous system or multisystem involvements.
JOURNAL OF NEUROLOGY
(2022)