Article
Microbiology
Yolanda Rivera-Cuevas, Joshua Mayoral, Manlio Di Cristina, Anna-Lisa E. Lawrence, Einar B. Olafsson, Romir K. Patel, Dishari Thornhill, Benjamin S. Waldman, Akira Ono, Jonathan Z. Sexton, Sebastian Lourido, Louis M. Weiss, Vern B. Carruthers
Summary: Toxoplasma gondii manipulates host cells to extract resources for its survival intracellularly, utilizing host ESCRT machinery with the involvement of transmembrane dense granule protein TgGRA14 to uptake host cytosolic proteins, revealing a novel mechanism for nutrient acquisition by the parasite.
Article
Chemistry, Multidisciplinary
Charles W. Hespen, Xiaohui Zhao, Howard C. Hang
Summary: This study evaluated the key properties involved in the formation of the Arf6-MDP-NOD2 complex in mammalian cells and found that the conserved Arf aromatic triad is crucial for binding to MDP-NOD2. Mutation of Arf6 N-myristoylation and NOD2 S-palmitoylation also disrupted the formation of the complex. Lipid-modified MDP was observed to increase Arf6-NOD2 assembly, suggesting that membrane targeting may be important in developing next-generation NOD2 agonists.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Huadong Jiang, Nannan Kong, Zeyuan Liu, Stephen C. West, Ying Wai Chan
Summary: Chromatin bridges between daughter nuclei during cell division can lead to DNA damage and chromosomal rearrangements. ANKLE1, a human endonuclease, accumulates at the midbody and prevents DNA damage and autoimmunity by cleaving chromatin bridges. ANKLE1 acts by priming the nucleolytic activity of TREX1 and inhibiting the formation of micronuclei and cytosolic dsDNA. This study highlights the importance of ANKLE1 in maintaining genomic stability and preventing autoimmune responses.
Article
Biology
Giovanni Messina, Yuri Prozzillo, Francesca Delle Monache, Maria Virginia Santopietro, Patrizio Dimitri
Summary: The subunits of the SRCAP and p400/Tip60 chromatin remodeling complexes relocate from chromatin to the mitotic apparatus during cell division, playing crucial roles in the proper execution of cell division.
Article
Biochemistry & Molecular Biology
Ji Eun Yu, Sun-Ok Kim, Jeong-Ah Hwang, Jin Tae Hong, Joonsung Hwang, Nak-Kyun Soung, Hyunjoo Cha-Molstad, Yong Tae Kwon, Bo Yeon Kim, Kyung Ho Lee
Summary: The study identifies a novel p-S60 epitope on beta-catenin generated by Plk1 kinase activity, which is crucial for the completion of cytokinesis. Depletion of beta-catenin leads to cytokinesis-defective phenotypes and apoptotic cell death, highlighting the importance of beta-catenin phosphorylation in cell division.
Article
Neurosciences
Yufu Zhu, Yang Xu, Tianze Chen, Yujian Zhang, Qiang Ma, Sunil Rauniyar, Lei Wang, Hengliang Shi
Summary: The study revealed that TSG101 is upregulated in glioma tissues, and high levels of TSG101 are associated with poor prognosis in glioma patients. Functional studies showed that TSG101 promotes the proliferation, migration, and invasion of glioma cells.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yen -Ming Lin, Pao-Hsien Chu, Pin Ouyang
Summary: CEP55 expression level is important for cell cytokinesis and tumor stage. TSG101 can decrease protein levels through lysosome degradation and interacts with UXT to modulate protein degradation. This study identified a novel pathway for CEP55 regulation by TSG101 overexpression via lysosome degradation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Rishith Ravindran, Anoop Kumar G. Velikkakath, Nikhil Dev Narendradev, Aneesh Chandrasekharan, T. R. Santhoshkumar, Srinivasa M. Srinivasula
Summary: This study reveals a novel role of endosomes in modulating upstream pathways of PRKN-dependent mitophagy initiation.
Review
Cell Biology
Leticia Labat-de-Hoz, Armando Rubio-Ramos, Javier Casares-Arias, Miguel Bernabe-Rubio, Isabel Correas, Miguel A. Alonso
Summary: Primary cilia are microtubule-based protrusions surrounded by a ciliary membrane equipped with selected receptors that control important signaling pathways in cell growth and development. This review presents a model of primary ciliogenesis by an alternative route involving the midbody remnant from cytokinesis, which delivers membranes to the centrosome for ciliary membrane assembly. The involvement of the midbody remnant in primary cilium formation and its regulation by the ESCRT machinery are discussed, along with the emerging roles of membrane compartmentalization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Virology
Longxiang Zhang, Rui Li, Rui Geng, Lei Wang, Xin-Xin Chen, Songlin Qiao, Gaiping Zhang
Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the swine industry. This study identifies tumor susceptibility gene 101 (TSG101), an ESCRT component, as an important factor in PRRSV infection. TSG101 plays a role in virion formation and subcellular localization of PRRSV N protein along the early secretory pathway. These findings suggest that TSG101 is a potential target for interfering with PRRSV infection.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Hui Tu, Zhimeng Wang, Ye Yuan, Xilin Miao, Dong Li, Hu Guo, Yihong Yang, Huaqing Cai
Summary: The study identifies a complex composed of two proteins, PripA and TbcrA, that plays a central role in the conversion of Rab5 to Rab7 during macropinosome maturation, facilitating efficient cargo trafficking.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Kia Wee Tan, Viola Nahse, Coen Campsteijn, Andreas Brech, Kay Oliver Schink, Harald Stenmark
Summary: The study identifies JIP4 and Phafin2 as crucial components in the recycling pathway from macropinosomes, with their interaction and impact on PtdIns3P affecting the retention and processing of internalized cargoes in cells.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biology
Alin Rai, David W. Greening, Rong Xu, Maoshan Chen, Wittaya Suwakulsiri, Richard J. Simpson
Summary: The study characterized the properties of secreted midbody remnants from colon cancer cells, showing they are a distinct class of membrane-encapsulated extracellular vesicle enriched in core cytokinetic proteins. These sMB-Rs are engulfed by quiescent fibroblasts and promote cellular transformation and invasive phenotypes. The authors found that sMB-Rs are different from exosomes and microparticles, and play a role in promoting anchorage independent growth and invasive phenotypes in fibroblasts.
COMMUNICATIONS BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Hyunji Byun, Sojung Kwon, Kay-Uwe Wagner, Hyejin Shin, Hyunjung Jade Lim
Summary: Deficiency of Tsg101 in uterine epithelium causes implantation failure and may be due to epithelial defects. The study found that UECs harbor a necroptotic machinery that responds to death-inducing signals.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Bettina Bohl, Ammar Jabali, Julia Ladewig, Philipp Koch
Summary: In human neural stem cells, lysosomes are asymmetrically inherited during mitosis and contain Notch receptors. Notch activation occurs in the acidic lysosome environment. The asymmetry of lysosomes correlates with Notch signaling activity in daughter cells.
