期刊
CELL STRESS & CHAPERONES
卷 19, 期 6, 页码 949-961出版社
SPRINGER
DOI: 10.1007/s12192-014-0519-2
关键词
Macrobrachium nipponense; Heat-shock protein; Heat-shock treatment; Aeromonas hydrophila; Immune response
类别
资金
- National Natural Sciences Foundation of China (NSFC) [31170120, 31101926, 31200139, 31272686]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX (12) 3066]
- Project for aquaculture in Jiangsu Province [PJ2011-65, Y2013-45, D2013-5-3, D2013-5-4]
- NSFC for Talents Training in Basic Science [J1103507]
The 70-kDa family of heat-shock proteins (Hsp70) plays an important role in the host immunity, which is widely expressed in eukaryotic cells as a major chaperone protein. In the present study, the full-length complementary DNA (cDNA) of a second cognate cytosolic Hsp70 family member (MnHsc70-2) was cloned and characterized from Macrobrachium nipponense, which is an economically and nutritionally important crustacean. The cDNA was 2,717 bp, containing an open reading frame (ORF) of 1,950 bp, which encodes a protein of 649 amino acids with a theoretical molecular weight of 71.1 kDa and an isoelectric point of 5.27. Sequence alignment showed that the MnHsc70-2 shared 75-97 % identity with other heat-shock proteins. Compared to the previously identified cognate Hsp70 (MnHsc70-1) in M. nipponense, MnHsc70-2 showed quite different expression profiles under unstressed conditions in all tested tissues, including the hemocytes, heart, hepatopancreas, gill, intestine, nerve, and muscle. The phylogenetic analysis demonstrated that MnHsc70-2 showed the closest relationship with MnHsc70-1. Heat-inducibility assays showed that two isolated messenger RNAs (mRNAs) displayed different expression profiles in both the hepatopancreas and gill tissues. MnHsc70-1 mRNA expression level decreased at first and then increased to the normal level, whereas MnHsc70-2 mRNA level increased at first and then decreased. The expressions of two MnHsc70s showed substantial obvious heat-inducible regulation in both the hepatopancreas and gill. Under bacterial challenge by Aeromonas hydrophila, both MnHsc70-1 and MnHsc70-2 mRNA level was up-regulated moderately. The results suggested that two cognate Hsc70s may play essential functions in mediating responses to heat-shock and bacterial challenge.
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