Article
Biochemistry & Molecular Biology
Mousam Roy, Koustav Bhakta, Arghya Bhowmick, Sayandeep Gupta, Anupama Ghosh, Abhrajyoti Ghosh
Summary: The study investigated the crosstalk in the heat shock response pathway of thermoacidophilic crenarchaeon Sulfolobus acidocaldarius, focusing on the small heat shock protein Hsp14. It was found that Hsp14 interacts with Hsp20 and Hsp60 to facilitate substrate proteins' folding under stress conditions, demonstrating the dynamic oligomeric structure of Hsp14 and its ability to form hetero-oligomers with Hsp20 at higher temperatures. Moreover, enthalpy-driven direct physical interaction between Hsp14 and Hsp60 was revealed, showing that Hsp14 can transfer substrate proteins captured by sHsp to Hsp60 for refolding.
Article
Chemistry, Physical
V. Carnevali, A. Sala, P. Biasin, M. Panighel, G. Comelli, M. Peressi, C. Africh
Summary: The electronic properties of graphene can be modified by local interaction with a metal substrate. This article proposes a reliable method of evaluating the interaction between graphene and substrate by testing the tunneling current. This method expands the capabilities of standard STM systems for studying graphene/substrate complexes.
Review
Biochemistry & Molecular Biology
Kevin Reinle, Axel Mogk, Bernd Bukau
Summary: The protein quality control system maintains protein homeostasis through substrate degradation, refolding, and sequestration activities, in which small heat shock proteins play a crucial role and adapt to different tasks and stress conditions.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Manuel Kleiner, Angela Kouris, Marlene Violette, Grace D'Angelo, Yihua Liu, Abigail Korenek, Nikola Tolic, Timo Sachsenberg, Janine McCalder, Mary S. S. Lipton, Marc Strous
Summary: In this study, a new protein-based stable isotope probing approach was developed, which allows for cost-effective analysis of associations between microbiome members and substrates. The approach also enables ultra-sensitive detection of stable isotopes and measurement of microbial activity under different diets.
Article
Parasitology
Nancy A. Aguoru, Ruth S. Kirk, Anthony J. Walker
Summary: This study provides a detailed molecular bioinformatic analysis of heat shock proteins (HSPs) in the human parasitic blood fluke Schistosoma mansoni. The results reveal the classification, expression patterns, protein interactions, and phosphorylation sites of HSPs in different stages of the parasite's life cycle. The findings suggest that HSPs play important roles in parasite survival and development, and offer insights for future investigations and therapeutics development against schistosomiasis.
PARASITES & VECTORS
(2022)
Review
Endocrinology & Metabolism
Zara Farooq, Lesley A. Howell, Peter J. McCormick
Summary: GPCRs not only exist as monomers, but also form complexes that modulate pharmacological responses and play important roles in diseases. Targeting protein-protein interactions presents challenges but offers potential strategies and techniques for research and development.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Editorial Material
Cell Biology
Christopher M. Jakobson, Daniel F. Jarosz
Summary: Cells reorganize their contents into subcellular structures in response to stimuli, with new research showing that proteins interact with substrates to coordinate disassembly of cellular structures and directly sense the cellular energy state for adaptive reorganization.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Digvijay Singh, Prajna Tripathi, Rahul Sharma, Sonam Grover, Janendra K. Batra
Summary: The N-terminal domain of Mtb ClpB plays a crucial role in the interaction with its co-partners and in the prevention of aggregation of proteins. The substrate binding pocket identified in this study, comprising of nine amino acid residues forming an alpha-helix, is important for the function of ClpB in repairing and resolubilizing aggregated proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Oncology
Ting Song, Yafei Guo, Zuguang Xue, Zongwei Guo, Ziqian Wang, Donghai Lin, Hong Zhang, Hao Pan, Xiaodong Zhang, Fangkui Yin, Hang Wang, Laura Bonnette Uwituze, Zhichao Zhang
Summary: S1g-2, a novel inhibitor targeting Hsp70-Bim PPI, exhibits higher apoptosis-inducing activity in CML and disrupts BCR-ABL-driven interaction, showing potential therapeutic value in both TKI-sensitive and resistant CML patients.
Article
Agricultural Engineering
Zhenyu Yue, Yinghong Wang, Nan Zhang, Bei Zhang, Yi Niu
Summary: This study identified the AaHsfA1 gene from Amorphophallus albus and found that it significantly improved the heat resistance of Arabidopsis under heat stress. Localization analysis showed that AaHsfA1 is located in the nucleus and cytoplasm. Protein interaction studies revealed direct interactions between AaHsfA1 and AaHsfA2a, AaHsp70, but not AaHsfB1, providing theoretical and genetic references for analyzing the mechanism of Amorphophallus heat resistance formation and crop resistance breeding.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Multidisciplinary Sciences
Tsukumi Miwa, Hideki Taguchi
Summary: Research has shown that IbpA down-regulates the expression of multiple heat shock proteins (Hsps) by repressing the translation of the heat shock transcription factor sigma(32), and plays a crucial role in shutting down the heat shock response. These findings reveal an unknown role of IbpA in regulating the heat shock response at the translational level.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Yunxie Wei, Hongqiu Zeng, Wen Liu, Xiao Cheng, Binbin Zhu, Jingru Guo, Haitao Shi
Summary: This study systematically analyzed the correlation between HSP90 and autophagy signaling in cassava, and investigated their effects on plant disease response and underlying mechanisms through functional genomics and genetic phenotype assay. The study found that the potential MeHSP90.9-MeSGT1-MeRAR1 chaperone complex interacts with MeATGs and triggers autophagy signaling, enhancing disease resistance to cassava bacterial blight.
Article
Biochemistry & Molecular Biology
Linan Xu, Hong Zhang, Daragh D. Cuskelly, Sean Doyle, Sarah Perrett, Gary W. Jones
Summary: Hsp70 is a conserved chaperone that plays a critical role in maintaining protein homeostasis. Mutations in the SBD disrupt its hydrophobic core, leading to impairment of heat-shock response and prion propagation. The disruption of key residues in the SBD region results in instability and cleavage of Hsp70, impacting its ATPase function and overall stability. This ultimately compromises the stress response and prion propagation abilities of yeast cells.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mousam Roy, Koustav Bhakta, Abhrajyoti Ghosh
Summary: Small heat shock proteins (sHsp) are a group of ATP-independent chaperones found in all three domains of life. The heat shock machinery in archaea is simplified, with a minimal repertoire of heat shock proteins. sHsps play a crucial role in preventing protein aggregation and stabilizing unfolded protein substrates. Furthermore, archaeal sHsps contribute to membrane stabilization and transferring substrate proteins to chaperonins. The similarities between archaeal and human sHsps make the former an excellent system for studying chaperonopathies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Plant Sciences
Yeeun Kang, Kwanuk Lee, Ken Hoshikawa, Myeongyong Kang, Seonghoe Jang
Summary: The effects of climate change, such as increased global temperatures and abnormal weather events, are causing concerns for plant vegetation and crop productivity. This review focuses on the molecular processes of plants in response to heat stress, with emphasis on the classification and functions of heat shock proteins. Developing vegetable crops with enhanced thermotolerance using genetic tools is crucial to alleviate the adverse effects of heat stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Javier A. Linares-Pasten, Peter Falck, Khalil Albasri, Sven Kjellstrom, Patrick Adlercreutz, Derek T. Logan, Eva Nordberg Karlsson
Article
Biochemistry & Molecular Biology
Gudrun Rutsdottir, Morten I. Rasmussen, Peter Hojrup, Katja Bernfur, Cecilia Emanuelsson, Christopher A. G. Soderberg
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2018)
Article
Hematology
Julia S. Westman, Linn Stenfelt, Karina Vidovic, Mattias Moller, Asa Hellberg, Sven Kjellstrom, Martin L. Olsson
Article
Hematology
Mattias Moller, Yan Quan Lee, Karina Vidovic, Sven Kjellstrom, Linda Bjorkman, Jill R. Storry, Martin L. Olsson
Article
Multidisciplinary Sciences
Rathi Saravanan, Daniel A. Holdbrook, Jitka Petrlova, Shalini Singh, Nils A. Berglund, Yeu Khai Choong, Sven Kjellstrom, Peter J. Bond, Martin Malmsten, Artur Schmidtchen
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Christopher A. G. Soderberg, Cecilia Mansson, Katja Bernfur, Gudrun Rutsdottir, Johan Harmark, Sreekanth Rajan, Salam Al-Karadaghi, Morten Rasmussen, Peter Hojrup, Hans Hebert, Cecilia Emanuelsson
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Yomna S. Mo'men, Rasha M. Hussein, Mohamed A. Kandeil
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Rosa S. Gisladottir, Erna Ivarsdottir, Agnar Helgason, Lina Jonsson, Nanna K. Hannesdottir, Gudrun Rutsdottir, Gudny A. Arnadottir, Astros Skuladottir, Benedikt A. Jonsson, Gudmundur L. Norddahl, Magnus O. Ulfarsson, Hannes Helgason, Bjarni Halldorsson, Muhammad S. Nawaz, Vinicius Tragante, Gardar Sveinbjornsson, Thorgeir Thorgeirsson, Asmundur Oddsson, Ragnar P. Kristjansson, Gyda Bjornsdottir, Gudmundur Thorgeirsson, Ingileif Jonsdottir, Hilma Holm, Daniel F. Gudbjartsson, Unnur Thorsteinsdottir, Hreinn Stefansson, Patrick Sulem, Kari Stefansson
Article
Biotechnology & Applied Microbiology
Daniel S. C. Butler, Caterina Cafaro, Johannes Putze, Murphy Lam Yim Wan, Thi Hien Tran, Ines Ambite, Shahram Ahmadi, Sven Kjellstrom, Charlotte Welinder, Sing Ming Chao, Ulrich Dobrindt, Catharina Svanborg
Summary: Bacterial protease can inhibit c-MYC protein in human cells, which leads to suppression of tumor cell proliferation and activity. This study provides a new direction and hope for targeted therapy of c-MYC.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rasha M. Hussein
Summary: Gum acacia mitigated hyperlipidemia and inflammation induced by Western diet through upregulation of miR-33 and miR-155, while having no effect on weight increase.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rasha M. Hussein, Saed M. Al-Dalain
Summary: The study found that betaine can protect against cisplatin-induced nephrotoxicity by modulating the expression of miRNA 34a, p53, apoptosis, and inflammation in rats.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Medicine, Research & Experimental
Rasha M. Hussein, Doaa M. Sawy, Mohamed A. Kandeil, Hatem S. Farghaly
Summary: Chlorogenic acid, quercetin, coenzyme Q10, and silymarin protect against TAA-induced acute liver toxicity through antioxidant, anti-inflammatory, anti-apoptotic activities, and regulating Keap1-Nrf2/HO-1 expression.
Article
Biochemistry & Molecular Biology
Rasha M. Hussein, Mohamed A. Kandeil, Norhan A. Mohammed, Rasha A. Khallaf
Summary: This study aimed to design Cur-loaded solid lipid nanoparticles (SLNs) and evaluate their hepatoprotective and antioxidant effects compared to raw Cur and NAC in a model of acute hepatotoxicity. The results showed that Cur-loaded SLNs effectively prevented liver damage by alleviating oxidative stress and inhibiting iNOS expression.
JOURNAL OF LIPOSOME RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Hildur M. Aegisdottir, Rosa B. Thorolfsdottir, Gardar Sveinbjornsson, Olafur A. Stefansson, Bjarni Gunnarsson, Vinicius Tragante, Gudmar Thorleifsson, Lilja Stefansdottir, Thorgeir E. Thorgeirsson, Egil Ferkingstad, Patrick Sulem, Gudmundur Norddahl, Gudrun Rutsdottir, Karina Banasik, Alex Hoerby Christensen, Christina Mikkelsen, Ole Birger Pedersen, Soren Brunak, Mie Topholm Bruun, Christian Erikstrup, Rikke Louise Jacobsen, Kaspar Rene Nielsen, Erik Sorensen, Michael L. Frigge, Kristjan E. Hjorleifsson, Erna Ivarsdottir, Anna Helgadottir, Solveig Gretarsdottir, Valgerdur Steinthorsdottir, Asmundur Oddsson, Hannes P. Eggertsson, Gisli H. Halldorsson, David A. Jones, Jeffrey L. Anderson, Kirk U. Knowlton, Lincoln D. Nadauld, D. B. D. S. Genomic Consortium DBDS Genomic Consortium, Magnus Haraldsson, Gudmundur Thorgeirsson, Henning Bundgaard, David O. Arnar, Unnur Thorsteinsdottir, Daniel F. Gudbjartsson, Sisse R. Ostrowsk, Hilma Holm, Kari Stefansson
Summary: This study investigates the genetics of syncope and provides insight into its pathophysiology and prognostic implications. The results show that syncope has a distinct genetic architecture involving neural regulatory processes and a complex relationship with heart rate and blood pressure regulation. There is also a shared genetic background with poor cardiovascular health. Thorough assessment is important for individuals presenting with syncope.
EUROPEAN HEART JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Rasha Hussein, Marwa Khalaf, Wafaa Mohamed
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
(2020)