期刊
CELL STEM CELL
卷 8, 期 4, 页码 371-375出版社
CELL PRESS
DOI: 10.1016/j.stem.2011.02.007
关键词
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资金
- Chinese Academy of Sciences [KSCX2-YIN-R-110, KSCX2-YW-R-229, XDA01010403]
- Ministry of Science and Technology [2007CB947101, 2009CB941101]
- National Natural Science Foundation of China [30871430]
- Shanghai Municipal Commission for Science and Technology [07DZ22919, 08DJ1400502, 09PJ1410900]
The low success rate of somatic nuclear transfer (NT) is hypothesized to be mainly due to functional defects in the trophoblast cell lineage rather than the inner cell mass (ICM); this hypothesis, however, remains to be tested directly. Here we separated the ICMs from cloned blastocysts and aggregated the cloned ICM with two fertilization-derived (FD) tetraploid (4N) embryos. We found that the full-term development of cloned ICMs was dramatically improved after the trophoblast cells in the cloned blastocysts were replaced by cells from tetraploid embryos, thus providing direct evidence that defects in trophoblast cell lineage underlie the low success rate of somatic NT.
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