期刊
CELL RESEARCH
卷 18, 期 10, 页码 1007-1019出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/cr.2008.286
关键词
Arabidopsis; chloroplast protein; network; functional linkage; photosynthesis
类别
资金
- National Natural Science Foundation of China [30530100, 90408010]
- State Key Program of Basic Research of China [2007CB947600, 2007CB108800]
- Hi-Tech Research and Development Program of China [2006AA02Z313]
Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22 925 protein interaction pairs which involved 2 214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem I (PSI), photosystem II (PSII), light harvesting complex of photosystem I (LHC I) and light harvesting complex of photosystem I (LHC II) could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis.
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