Article
Multidisciplinary Sciences
Samuel M. Law, Jie J. Zheng
Summary: Wnt signaling pathways play a significant role in various diseases. β-catenin is central to the canonical Wnt signaling pathway. CHIR-99021, a GSK-3β inhibitor, is widely used for studying this pathway. However, caution is needed when using CHIR-99021 at certain concentrations.
Article
Cell & Tissue Engineering
Koki Kimura, Shiori Yamamori, Masaharu Hazawa, Jingjing Kobayashi-Sun, Mao Kondo, Richard W. Wong, Isao Kobayashi
Summary: Using a stromal cell line from the zebrafish kidney, researchers found that an inhibitor of canonical Wnt signaling, IWR-1-endo, is a potent regulator of hematopoietic stem cells (HSCs). The stromal cells weakly expressed HSC maintenance factors but highly expressed canonical Wnt ligand genes. Adding IWR-1-endo or inhibiting canonical Wnt signaling increased the number of HSCs.
Article
Biochemistry & Molecular Biology
Nihal AlMuraikhi, Sarah Binhamdan, Hanouf Alaskar, Amal Alotaibi, Sumaiya Tareen, Manikandan Muthurangan, Musaad Alfayez
Summary: In this study, 1Azakenpaullone was identified as a highly selective inhibitor that induced osteoblastic differentiation and mineralization of human mesenchymal stem cells (MSCs) by activating the Wnt signaling pathway and nuclear accumulation of beta-catenin, leading to the upregulation of Runx2 and the expression of osteoblast-specific genes. Therefore, 1Azakenpaullone could be used as an osteo-promoting factor in bone tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Sybille Landwehr-Kenzel, Leonie Mueller-Jensen, Joern-Sven Kuehl, Mohamed Abou-el-Enein, Henrike Hoffmann, Sandra Muench, Daniel Kaiser, Andy Roemhild, Horst von Bernuth, Mirjam Voeller, Michael Schmueck-Henneresse, Bernd Gruhn, Ulrik Stervbo, Nina Babel, Hans-Dieter Volk, Petra Reinke
Summary: This study explored the use of adoptive transfer of ex vivo expanded regulatory T cells (Tregs) for the treatment of chronic graft-versus-host disease (GvHD). The results showed that Tregs therapy was well tolerated and capable of modulating established adverse T cell mediated allo-response in patients.
Review
Biochemistry & Molecular Biology
Beatrice Balboni, Mirco Masi, Walter Rocchia, Stefania Girotto, Andrea Cavalli
Summary: Most kinase inhibitors target ATP-binding sites, resulting in promiscuity and potential off-target effects. Allostery provides an alternative approach to achieve selectivity, but its exploitation is challenging due to diverse mechanisms and long-range conformational effects. GSK-3 beta, a critical target involved in multiple pathologies, shares a highly homologous ATP-binding site with other kinases. Allostery offers the potential for selective and moderate inhibition, which is desirable for GSK-3 beta. However, the development of allosteric inhibitors for GSK-3 beta has been limited, with only one reaching clinical trials, and there are no X-ray structures of GSK-3 beta with allosteric inhibitors in the PDB data bank.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Shanshan Liu, Xiaoxi Lv, Xupeng Wei, Chang Liu, Qiao Li, Jiali Min, Fang Hua, Xiaowei Zhang, Ke Li, Pingping Li, Yang Xiao, Zhuowei Hu, Bing Cui
Summary: This study reveals an intact profibrotic axis of TRIB3-GSK-3 beta-A20-C/EBP beta in alveolar macrophages, which represents a target that may provide a promising treatment strategy for pulmonary fibrosis.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Engineering, Biomedical
Kelcee A. Everette, Gregory A. Newby, Rachel M. Levine, Kalin Mayberry, Yoonjeong Jang, Thiyagaraj Mayuranathan, Nikitha Nimmagadda, Erin Dempsey, Yichao Li, Senthil Velan Bhoopalan, Xiong Liu, Jessie R. Davis, Andrew T. Nelson, Peter J. Chen, Alexander A. Sousa, Yong Cheng, John F. Tisdale, Mitchell J. Weiss, Jonathan S. Yen, David R. Liu
Summary: Prime editing efficiently corrects the sickle-cell allele in patient haematopoietic stem cells, resulting in erythrocytes resistant to hypoxia-induced sickling. This correction was achieved at frequencies of 15%-41% in patients with sickle-cell disease. After transplantation into mice, the prime-edited cells maintained normal gene expression and displayed similar differentiation and maturation as unedited stem cells from healthy donors.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Medicine, Research & Experimental
Haoqi Zhao, Lan Wang, Shufang Wang, Xihua Chen, Min Liang, Xin Zhang, Jiedong Wang, Xiangbo Xu
Summary: The study revealed that the overexpression of CHN1 is associated with lymph node metastasis and low survival rates in cervical carcinoma patients. CHN1 overexpression promoted cell proliferation, migration, and invasion by inducing EMT-related transcription factors. Furthermore, in vitro experiments showed that CHN1 overexpression activated the Akt/GSK-3 beta/Snail signaling pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Qing Wang, Wei Zhang, Xiaole Peng, Yunxia Tao, Ye Gu, Wenming Li, Xiaolong Liang, Liangliang Wang, Zerui Wu, Tianhao Wang, Haifeng Zhang, Xin Liu, Yaozeng Xu, Yu Liu, Jun Zhou, Dechun Geng
Summary: Wear particle-induced periprosthetic osteolysis poses a severe threat to global public health and is a major cause of joint arthroplasty failure and secondary surgery. The study reveals that the Hh-Gli1 signaling cascade, mediated through GSK-3 beta, is involved in titanium nanoparticle-induced osteolysis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Physiology
Stan Moaraf, Ido Rippin, Joseph Terkel, Hagit Eldar-Finkelman, Anat Barnea
Summary: Translation: Glycogen synthase kinase-3 (GSK-3) plays a significant role in a variety of cellular processes, cognition, and behavior. In this study, the role of GSK-3 beta in social behavior, motivation, and motor activity in zebra finches was investigated. The inhibition of GSK-3 beta affected social recognition, induced anxiety-like behaviors, influenced decision-making, increased motor activity, and enhanced motivation to join flock members. This research contributes to a better understanding of GSK-3 and its potential implications for cognition and behavior.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yi Yang, Yuanyuan Li, Shaoqiong Zhang, Linyan Cao, Yansong Zhang, Bo Fang
Summary: This study demonstrates that exosomes derived from bone marrow mesenchymal stem cells (BMSCs) can promote neural stem cell (NSC) proliferation through the enrichment of miR-199a-5p. The miR-199a-5p exosomes target and regulate GSK-3 beta/beta-catenin signaling, leading to increased NSC proliferation.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Article
Cell & Tissue Engineering
Huangfan Xie, Zhongjie Sun, Xiong Xiao, Defang Liu, Hailong Qi, Guoxiong Tian, Miao Chen, Ligong Chen, Xuncheng Su
Summary: Transient inhibition of the JNK pathway can increase the frequency of HSCs in cord blood and promote their quiescence, leading to the enhancement of HSC number and engraftment potential. These findings have the potential to improve HSC collection and transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Hui Liu, Mangaladoss Fredimoses, Peijia Niu, Tingting Liu, Yan Qiao, Xueli Tian, Xiaobing Chen, Dong Joon Kim, Xiang Li, Kangdong Liu, Zigang Dong
Summary: EPRS is frequently overexpressed in GC tissues and its overexpression is associated with poor prognosis in GC patients. High expression of EPRS positively correlates with GC development both in vitro and in vivo by enhancing the WNT/GSK-3 beta/beta-catenin signaling pathway. XA and 4-HD can directly bind to EPRS to block this signaling pathway and inhibit gastric cancer growth.
Article
Chemistry, Multidisciplinary
You-hui Yu, Yu-hong Zhang, Yan-qing Ding, Xue-ying Bi, Jing Yuan, Hang Zhou, Pan-xia Wang, Li-li Zhang, Jian-tao Ye
Summary: The study revealed that miR-99b-3p contributes to cardiac fibrosis induced by Ang II, partially through regulating GSK-3 beta. Upregulation of miR-99b-3p promotes fibrotic responses, while inhibition of miR-99b-3p exerts anti-fibrotic effects.
ACTA PHARMACOLOGICA SINICA
(2021)
