期刊
CELL DEATH AND DIFFERENTIATION
卷 20, 期 3, 页码 382-395出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/cdd.2012.140
关键词
apoptosis; blebbishields; cancer stem cells; VEGFR2; p70S6K; caspase
资金
- MD Anderson Cancer Center
- Clayton Foundation for Research
- National Institutes of Health through MD Anderson Cancer Center's Support Grant [CA016672]
Caspases mediate apoptosis and have also been implicated in stem-cell biology. How caspases are linked to stem-cell biology is not known. Here, we show that the apoptotic blebs of cancer cells fuse together to form novel structures called 'blebbishields'. Blebbishields form spheres by fusion. Both blebbishield formation and sphere formation involve active caspases and N-linked glycosylation. Sphere formation is enhanced by acidic pH and is counteracted by inhibitors of proton pump, caspases, and cholesterol. The blebbishields from VEGFR2(High) cells are capable of enhanced sphere formation. Blebbishields express transiently downregulated stem-cell markers and the sphere-forming blebbishield-derived cells are tumorigenic. Our study demonstrates that the cancer stem cells can survive after apoptosis by blebbishield formation and subsequent sphere formation. Cell Death and Differentiation (2013) 20, 382-395; doi:10.1038/cdd.2012.140; published online 23 November 2012
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据