Review
Biochemistry & Molecular Biology
Laura Monaghan, Dasa Longman, Javier F. Caceres
Summary: mRNA surveillance pathways play a crucial role in maintaining accurate gene expression and translation homeostasis. Studying these pathways can help us understand how cellular mRNA levels are regulated, how defective mRNAs are eliminated, and how dysregulation can contribute to human disease.
Article
Biochemistry & Molecular Biology
Ankita Agarwal, Suresh Alagar, Shri Kant, Ranjit Prasad Bahadur
Summary: This study investigates the molecular mechanism of interaction between the N-terminal tandem RNA-recognition motifs (tRRMs) of HuR and mRNA using molecular dynamics simulation. The researchers also explore the effect of point mutations on the binding specificity of HuR-mRNA interactions. The findings reveal that certain mutations significantly affect the binding affinity due to unfavorable conformations and loss of critical interactions.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Zarmik Moqtaderi, Joseph V. Geisberg, Kevin Struhl
Summary: There is a compensatory link between cleavage/polyadenylation in the nucleus and mRNA turnover in the cytoplasm. Same-gene 30 mRNA isoforms with different half-lives have similar steady-state mRNA levels, indicating an inverse relationship between cleavage/polyadenylation and mRNA isoform stability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Haneui Bae, Jeff Coller
Summary: Translation by the ribosome is the final step in the process of producing proteins from DNA. Recent research has shown that the ribosome also plays a role in controlling mRNA stability and degradation. The rate at which the ribosome moves along the mRNA molecule affects the stability of the transcript. This process is influenced by the codon sequence, with some codons causing the ribosome to pause and adopt a specific conformation that triggers mRNA degradation. These findings suggest that the coding region of mRNA not only determines protein content, but also impacts protein levels by influencing the fate of the transcript.
Article
Biology
Anthony J. Veltri, Karole N. D'Orazio, Laura N. Lessen, Raphael Loll-Krippleber, Grant W. Brown, Rachel Green
Summary: This study investigates the role of Syh1 and Smy2 in coordinating translation repression and mRNA decay in Saccharomyces cerevisiae. The authors demonstrate that the Syh1/Smy2-mediated pathway acts redundantly and compensates for impaired Hel2-dependent NGD. Importantly, the NGD factors identified in this study are not involved in the degradation of mRNAs enriched in non-optimal codons.
Article
Biochemistry & Molecular Biology
Yueshuang Ke, Xueping Lv, Xingyue Fu, Jing Zhang, Ameer Ali Bohio, Xianlu Zeng, Wenjing Hao, Ruoxi Wang, Istvan Boldogh, Xueqing Ba
Summary: PARylation is an important post-translational modification that increases the formation of HuR oligomer/multimer, which in turn promotes the disassociation of miRISC and stabilizes pro-inflammatory gene mRNAs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ravi Kumar, Dipak Kumar Poria, Partho Sarothi Ray
Summary: Posttranscriptional regulation of gene expression is crucial in controlling the inflammatory response and preventing tumorigenesis. The interactions between HuR and La proteins play a cooperative role in regulating PDCD4 mRNA translation and mitigating the oncogenic function of miR-21. This interplay demonstrates a synergistic effect on PDCD4 expression, helping maintain balance between inflammation and tumorigenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Shengtao Wang, Xinxin Zeng, Peng Gui, Shujuan Xu, Zhaoxu Li, Dongxu Chen
Summary: This study found that long non-coding RNA (lncRNA) EBLN3P promoted the proliferative, migrative, and invasive potentials of osteosarcoma (OS) cells by increasing the stability and protein level of Annexin A3 (ANXA3) mRNA through recruiting HuR. This discovery provides new potential therapeutic targets for clinical treatment of OS.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Mehdi Badaoui, Cyril Sobolewski, Alexandre Luscher, Marc Bacchetta, Thilo Kohler, Christian van Delden, Michelangelo Foti, Marc Chanson
Summary: Cystic fibrosis (CF) is a chronic respiratory disease caused by bacterial infections, with Pseudomonas aeruginosa (Pa) being the main pathogen. The overexpression of Vav3, a guanine nucleotide exchange factor, is induced by the upregulation of mRNA-stabilizing protein HuR in CF patients. The accumulation of HuR in the cytoplasm of CF airway epithelial cells stabilizes Vav3 mRNA, leading to the formation of fibronectin-made bacterial docking platforms and Pa adhesion. Targeting HuR could be a promising strategy to prevent Pa infection in CF.
Article
Biochemistry & Molecular Biology
Qinfang Liu, Dong Zhu, Naren Li, Shifan Chen, Liang Hu, Jianzhong Yu, Yulan Xiong
Summary: This study identifies the role of the purine biosynthesis pathway enzyme ATIC in regulating LRRK2 protein levels and toxicity. The precursor AICAr can regulate LRRK2 levels in a cell-type-specific manner in vitro and in mouse tissue. AICAr regulates LRRK2 levels through AUF1-mediated mRNA decay. This study provides insights into a novel regulatory mechanism of LRRK2 protein levels and function, independent of its enzymatic activity.
Article
Biochemistry & Molecular Biology
Yanjin Sun, Siyuan Zhan, Sen Zhao, Tao Zhong, Linjie Wang, Jiazhong Guo, Dinghui Dai, Dandan Li, Jiaxue Cao, Li Li, Hongping Zhang
Summary: HuR is highly expressed in goat skeletal muscle and plays a crucial role in promoting muscle differentiation by increasing the stability of Myomaker mRNA. Overexpression of HuR accelerates muscle cell differentiation and formation of myotubes, while knockdown of HuR has the opposite effects. HuR binds to Myomaker, increasing its mRNA stability and positively regulating its expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xiaojin Song, Bing Chen, Yiran Liang, Yaming Li, Hanwen Zhang, Dianwen Han, Yajie Wang, Fangzhou Ye, Lijuan Wang, Wenjing Zhao, Qifeng Yang
Summary: This study identified a novel circRNA, circEIF3H, with significant cancer-promoting function in the progression of TNBC. It acts as a scaffold for IGF2BP2/HuR to regulate mRNA stability. This finding provides a potential target for developing individualized therapy for TNBC.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Tingjia Zhu, Yixuan Cen, Zhuoye Chen, Yanan Zhang, Lu Zhao, Jiaying Wang, Weiguo Lu, Xing Xie, Xinyu Wang
Summary: This study identified the overexpression of circTICRR in cervical cancer. CircTICRR interacts with HuR protein, stabilizes GLUD1 mRNA, and promotes cell apoptosis and inhibits proliferation through autophagy activation.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Brenda Janice Sanchez, Souad Mubaid, Sandrine Busque, Yossef Lopez de los Santos, Kholoud Ashour, Jason Sadek, Xian Jin Lian, Shahryar Khattak, Sergio Di Marco, Imed-Eddine Gallouzi
Summary: mRNA stability is crucial for cell function and fate, and this study identifies YB1 as a critical partner of HuR in stabilizing mRNA and promoting myogenesis. HuR and YB1 form a complex that binds to common mRNA targets, particularly those with a U-rich consensus motif in their 3' UTR. This finding highlights the complexity of the mRNA stability mechanism and its importance in cellular processes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Medicine, Research & Experimental
Jiaxin Chen, Yizheng Wu, Xin Luo, Dongai Jin, Wei Zhou, Zhenyu Ju, Di Wang, Qing Meng, Huijuan Wang, Xiaotian Fu, Jianbin Xu, Zhangfa Song
Summary: circRHOBTB3 is downregulated in colorectal cancer tissues and cell lines, overexpression of circRHOBTB3 suppresses tumor metastasis through the HuR/PTBP1 axis in CRC.
Correction
Biochemistry & Molecular Biology
Annapina Russo, Morena Catillo, Davide Esposito, Paola Briata, Concetta Pietropaolo, Giulia Russo
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Silvia Bottini, Nedra Hamouda-Tekaya, Bogdan Tanasa, Laure-Emmanuelle Zaragosi, Valerie Grandjean, Emanuela Repetto, Michele Trabucchi
NUCLEIC ACIDS RESEARCH
(2017)
Article
Multidisciplinary Sciences
Marta Geretto, Marco Ponassi, Martina Casale, Alessandra Pulliero, Grazia Cafeo, Ferdinando Malagreca, Aldo Profumo, Enrica Balza, Rakhmetkazhi Bersimbaev, Franz Heinrich Kohnke, Camillo Rosano, Alberto Izzotti
SCIENTIFIC REPORTS
(2018)
Article
Pharmacology & Pharmacy
Silvana Alfei, Silvia Catena, Marco Ponassi, Camillo Rosano, Vittoria Zoppi, Andrea Spallarossa
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2018)
Article
Chemistry, Medicinal
Carmela Saturnino, Anna Caruso, Domenico Iacopetta, Camillo Rosano, Jessica Ceramella, Noemi Muia, Annaluisa Mariconda, Maria Grazia Bonomo, Marco Ponassi, Giuseppe Rosace, Maria Stefania Sinicropi, Pasquale Longo
Review
Genetics & Heredity
Michele Trabucchi
TRENDS IN GENETICS
(2019)
Article
Multidisciplinary Sciences
Martina Rossi, Gabriele Bucci, Dario Rizzotto, Domenico Bordo, Matteo J. Marzi, Margherita Puppo, Arielle Flinois, Domenica Spadaro, Sandra Citi, Laura Emionite, Michele Cilli, Francesco Nicassio, Alberto Inga, Paola Briata, Roberto Gherzi
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Vera L. Costa, Jean-Bernard Ruidavets, Vanina Bongard, Bertrand Perret, Emanuela Repetto, Maria G. Stathopoulou, Fabrizio Serra, Mohamed Benahmed, Claire Mauduit, Valerie Grandjean, Jean Ferrieres, Laurent O. Martinez, Michele Trabucchi
Summary: The study shows that the elevated expression of serum s-RNY1-5p is associated with an increased risk of coronary heart disease (CHD) events in the male population, suggesting its potential as a predictive biomarker for detection of cardiovascular diseases.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Georges Raad, Fabrizio Serra, Luc Martin, Marie-Alix Derieppe, Jerome Gilleron, Vera L. Costa, Didier F. Pisani, Ez-Zoubir Amri, Michele Trabucchi, Valerie Grandjean
Summary: Research shows that maintaining a paternal Western diet feeding for five consecutive generations in mice leads to an increase in fat mass and related metabolic diseases over generations, but progenies from these multigenerational Western-diet-fed males develop a healthy overweight phenotype characterized by normal glucose metabolism and without fatty liver, which persists for four subsequent generations. Sperm RNA is suggested to be sufficient for the establishment but not for long-term maintenance of epigenetic inheritance of metabolic pathologies.
Article
Biochemical Research Methods
Silvia Bottini, David Pratella, Valerie Grandjean, Emanuela Repetto, Michele Trabucchi
BRIEFINGS IN BIOINFORMATICS
(2018)
Review
Andrology
Georges Raad, Mira Hazzouri, Silvia Bottini, Michele Trabucchi, Joseph Azoury, Valerie Grandjean
BASIC AND CLINICAL ANDROLOGY
(2017)
Article
Biochemistry & Molecular Biology
Arfa Moshiri, Margherita Puppo, Martina Rossi, Roberto Gherzi, Paola Briata
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2017)
Article
Biochemistry & Molecular Biology
Isabella Panfoli, Marco Ponassi, Silvia Ravera, Daniela Calzia, Maider Beitia, Alessandro Morelli, Camillo Rosano
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)