Article
Oncology
Ana P. Ferragut Cardoso, Mayukh Banerjee, Alexandra N. Nail, Angeliki Lykoudi, J. Christopher States
Summary: Genomic instability, caused by genetic alterations, leads to tumor heterogeneity and drug resistance, resulting from defects in DNA damage surveillance mechanisms and repair machinery. miRNA dysregulation may play a crucial role in mediating genome instability and carcinogenesis.
SEMINARS IN CANCER BIOLOGY
(2021)
Review
Cell Biology
Sisi Chen, Xinwei Geng, Madiha Zahra Syeda, Zhengming Huang, Chao Zhang, Songmin Ying
Summary: MUS81 plays a significant role in DNA repair but is dispensable for embryonic development and cell viability in mammals. Discrepant gene mutations completely alter the role of MUS81 in human cancers, leading to potential changes in therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lara Barrio, Ana -Elena Gaspar, Mariana Muzzopappa, Kaustuv Ghosh, Daniela Romao, Marta Clemente-Ruiz, Marco Milan
Summary: This study demonstrates that DNA damage resulting from lagging chromosomes during mitosis and aneuploidy-induced replicative stress contributes to the increased invasiveness associated with chromosomal instability. The study also highlights the role of effector caspases and the JAK/STAT signaling pathway in enhancing DNA damage and promoting CIN-induced invasiveness. Additionally, an autocrine feedforward amplification loop mediated by Upd3 and the JAK/STAT signaling pathway is found to contribute to the activation of the apoptotic pathway in migrating cells, further promoting CIN-induced invasiveness.
Review
Biochemistry & Molecular Biology
Mengting Chen, Renske Linstra, Marcel A. T. M. van Vugt
Summary: Genomic and chromosomal instability are characteristics of cancer that affect the genetic composition of cancer cells, influencing their behavior and response to treatment. This instability can lead to DNA leakage into the cytoplasm, triggering inflammatory signaling pathways that have pleiotropic effects, including enhanced anti-tumor immunity and potential sensitization of cancer cells to immune checkpoint inhibitors.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Cell Biology
Swati Priya, Ekjot Kaur, Swati Kulshrestha, Awadhesh Pandit, Isabelle Gross, Nitin Kumar, Himanshi Agarwal, Aamir Khan, Radhey Shyam, Prakash Bhagat, Jyothi S. Prabhu, Perumal Nagarajan, S. V. S. Deo, Avinash Bajaj, Jean-Noel Freund, Arnab Mukhopadhyay, Sagar Sengupta
Summary: A DNA damage responsive miR signature called DNA damage sensitive miRs (DDSMs) was identified in colon adenocarcinoma patient tissues, which were upregulated by the intestine-specific transcription factor CDX2 and repressed by HDAC1/2-containing complexes. These miRs downregulated key targets in the DNA damage response pathway and were associated with decreased survival probability in colon cancer patients, showing potential as a prognostic biomarker.
JOURNAL OF CELL SCIENCE
(2021)
Article
Toxicology
Guadalupe M. Vedoya, Marcela M. Lopez Nigro, Gabriela A. Martin
Summary: In this study, the effect of non-tumor mammary cell secretome on breast cancer cells was evaluated, showing that it can induce DNA damage and chromosomal abnormalities, as well as increase chromosomal instability. Clonogenic survival of tumor cells was also modulated by the secretome, indicating the significance of tumor-host interaction in tumor behavior and response to radiotherapy.
TOXICOLOGY IN VITRO
(2021)
Article
Oncology
Luca Mattiello, Sara Soliman Abdel Rehim, Martina Musella, Antonella Sistigu, Andrea Guarracino, Sara Vitale, Francesca Corradi, Claudia Galassi, Francesca Sperati, Gwenola Manic, Ruggero De Maria, Ilio Vitale
Summary: The targeting of RAD51 or MRE11 improves the sensitivity of primary CRC-SCs to CHK1/2 inhibitors by inducing replication stress, abrogating cell cycle checkpoints, and causing mitotic defects, leading to mitotic catastrophe and apoptosis. This approach may offer a promising strategy for eradicating cancer stem cells in colorectal cancer patients.
Article
Multidisciplinary Sciences
Franklin Mayca Pozo, Xinran Geng, Ilaria Tamagno, Mark W. Jackson, Ernest G. Heimsath, John A. Hammer, Richard E. Cheney, Youwei Zhang
Summary: MYO10 overexpression in tumors increases genomic instability, induces an inflammatory response, and accelerates tumor growth, while depletion of MYO10 reduces genomic instability and inflammation signaling, slowing down tumor progression. MYO10 promotes tumor progression by inducing genomic instability, which creates an immunogenic environment for immune checkpoint blockades.
Article
Chemistry, Medicinal
Zhongren Xu, Qiuyue Lu, Min Shan, Guizhi Jiang, Yuanhao Liu, Zhibin Yang, Yunlong Lu, Wukun Liu
Summary: Inflammation promotes ovarian cancer development, and chemoresistance is a major challenge in its treatment. A series of gold(I) complexes derived from NSAIDs were synthesized, and complex B3 (Npx-Au) exhibited higher antitumor activity than cisplatin and other gold(I) complexes. Npx-Au induced oxidative stress and the DAMPs process by inhibiting TrxR activity. Mechanistic studies showed simultaneous downregulation of COX-2 and PD-L1 after Npx-Au treatment. Interestingly, in vivo experiments demonstrated that Npx-Au treatment stimulated immune response by reducing PD-L1 expression, inducing DC maturation, and increasing T cell infiltration. Overall, Npx-Au elicited immunogenic cell death and showed promise as a strategy for chemotherapy combined with immunotherapy in treating ovarian cancer.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Oncology
I Lodewijk, A. Bernardini, C. Suarez-Cabrera, E. Bernal, R. Sanchez, J. L. Garcia, K. Rojas, L. Morales, S. Wang, X. Han, M. Duenas, J. M. Paramio, L. Manso
Summary: In patients with ovarian cancer undergoing neoadjuvant chemotherapy, we found that higher levels of genomic loss of heterozygosity were associated with a more significant tumor response to chemotherapy. Additionally, neoadjuvant therapy increased the population of tumor-infiltrating lymphocytes, primarily CD8+ T cells. These findings shed light on the dynamic interaction between tumors and the immune system, suggesting the potential use of immune checkpoint inhibitors or their combination with poly-ADP polymerase inhibitors as a treatment strategy for high-stage and grade epithelial ovarian cancer patients undergoing neoadjuvant therapy.
NPJ PRECISION ONCOLOGY
(2022)
Article
Oncology
Sweta Sharma Saha, Lucy Gentles, Alice Bradbury, Dominik Brecht, Rebecca Robinson, Rachel O'Donnell, Nicola J. Curtin, Yvette Drew
Summary: This study identified that genomic/transcriptomic and/or functional alterations in DDR pathways can determine therapy response, offering insights into personalized chemotherapy choices for ovarian cancer patients.
Editorial Material
Multidisciplinary Sciences
Craig M. Bielski, Barry S. Taylor
Summary: Genomic instability is a characteristic of cancer, but exploiting this feature to selectively target cancer cells remains a major challenge in cancer biology with significant implications for drug development.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Weiwei Xie, Huizhen Sun, Xiaoduan Li, Feikai Lin, Ziliang Wang, Xipeng Wang
Summary: Ovarian cancer is associated with activation of oncogenes and inactivation of tumor suppressor genes, with epigenetic processes playing a key role in tumorigenesis. MicroRNA and long non-coding RNA are promising molecular markers for precision medicine in OC. Clinical application of epigenetic approaches is currently limited by hurdles such as heterogeneity of OC and insufficient sample sizes, but holds promise for future clinical investigation into diagnosis, treatment, and prognosis.
CANCER CELL INTERNATIONAL
(2021)
Review
Immunology
Hongyu Xie, Wenjie Wang, Wencai Qi, Weilin Jin, Bairong Xia
Summary: Combining DDR inhibitors with ICI therapy in ovarian cancer can enhance local antigen release, promote systemic antitumor immune responses, and potentially improve clinical outcomes for patients with ovarian cancer. This combination therapy strategy is based on the interconnectivity between DDR pathway deficiency and immune response, and it is supported by available clinical trials and potential predictive biomarkers.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Josef Horak, Alexandra Dolnikova, Ozge Cumaogullari, Andrea Cumova, Nazila Navvabi, Ludmila Vodickova, Miroslav Levy, Michaela Schneiderova, Vaclav Liska, Ladislav Andera, Pavel Vodicka, Alena Opattova
Summary: Cancer therapy failure is a major challenge in cancer treatment, with acquired resistance being one of the most common reasons for it. This study suggests that miR-140 may act as a tumor suppressor and play an important role in the response to oxaliplatin therapy in colorectal cancer.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biology
Adil Khan, Arif Ahmed Muhammed, Nasreen Nasreen, Furhan Iqbal, Raquel Cossio-Bayugar, Said Sajjad Ali Sha, Abdullah D. Alanazi, Zbigniew Zajac
Summary: Livestock industry plays a vital role in Pakistan's economy, however, small ruminants are at risk of haemoparasitic diseases transmitted by ticks. There is limited knowledge about tick-borne diseases and their vectors in goats and sheep in Pakistan, highlighting the need for further research and control measures.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biology
Shahzadi Noor Ul Ain Zafar, Adil Khan, Sadaf Niaz, Munir Aktas, Sezayi Ozubek, Muhammad Farooq, Muhammad Moeen Adil, Zbigniew Zajac, Furhan Iqbal, Ahmad R. Alhimaidi, Ayman A. Swelum
Summary: Anaplasmosis caused by Anaplasma marginale is a frequently reported tick-borne disease in tropical and sub-tropical countries. This study examined the prevalence and phylogenetic origin of A. marginale in cattle blood samples from two livestock regions in Punjab Province, Pakistan. The results showed a higher prevalence of A. marginale in cattle from Dera Ghazi Khan compared to Lodhran, and all cattle breeds in both districts were equally susceptible to the infection.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Genetics & Heredity
Jianing Gao, Huan Zhang, Xiaohua Jiang, Asim Ali, Daren Zhao, Jianqiang Bao, Long Jiang, Furhan Iqbal, Qinghua Shi, Yuanwei Zhang
Summary: FertilityOnline is a database that integrates functional genes related to spermatogenesis, facilitating the exploration of the genetic basis of male infertility.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Parasitology
Muhammad Naveed Aziz, Rana Khalid Iqbal, Muhammad Irfan, Asia Parveen, Muhammad Asif, Sezayi Ozubek, Munir Aktas, Mourad Ben Said, Furhan Iqbal
Summary: Raising small ruminants is crucial for farmers' income in Pakistan. This study investigates the seasonal and molecular prevalence of T. gondii in different breeds of goats in Khanewal district, Punjab, Pakistan. The results show a relatively low prevalence of T. gondii, with the highest prevalence observed in summer. Factors such as male goats, neighboring farms with other dairy animals, and water supply from pools are associated with higher infection rates.
Article
Immunology
Iram Taalay, Rana Khalid Iqbal, Muhammad Asif, Aqib Ahmad, Muhammad Amjad, Farhana Naureen Anwar, Munir Aktas, Mourad Ben Said, Furhan Iqbal
Summary: This study investigated the prevalence and genetic diversity of Toxoplasma gondii in large ruminants in Pakistan. The results showed a natural infection of T. gondii in cattle for the first time in Pakistan. The findings are important for designing control strategies to improve the livestock sector, which is a main source of income in Pakistan.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Huijuan Wen, Fazhan Li, Ihtisham Bukhari, Yang Mi, Chenxu Guo, Bin Liu, Pengyuan Zheng, Simeng Liu
Summary: This study aims to explore immune targets in colorectal cancer for improved treatment outcomes and prognosis. By classifying tumors into hot and cold based on immune cell infiltration score and gene expression differences, 12 immune-related hub genes were identified using LASSO regression analysis, with CYSLTR2 identified as a potential candidate gene. A prognostic model was constructed and validated, showing promising potential of CYSLTR2 as an important immune target for the prognosis of colorectal cancer.
Article
Medicine, General & Internal
Hira Jamil, Adeela Awan, Atif Akbar, Muhammad Babar, Sana Akhtar, Rana Khalid Iqbal, Furhan Iqbal
Summary: This study found a significant association between the presence of rs1799883 in the fatty acid binding protein-2 gene and the absence of the glutathione S-transferase mu gene with the development of type 2 diabetes.
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION
(2022)
Article
Microbiology
Salama Al-Hamidhi, Asia Parveen, Furhan Iqbal, Muhammad Asif, Naheed Akhtar, Elshafie Elshafie, Albano Beja-Pereira, Hamza A. Babiker
Summary: The study found that the genetic structure of Theileria annulata in Pakistan is similar to that in Oman, Tunisia, and Turkey, showing a high level of genetic diversity. The genotypes of the parasite in these countries are geographically sub-structured. The parasite populations in Oman, Indian Subcontinent (Pakistan), Africa (Tunisia), and Europe (Turkey) overlap, indicating some level of parasite gene flow between neighboring countries.
Article
Parasitology
Nusrat Yasmeen, Muhammad Kashif, Tehreem Arif, Adil Khan, Muhammad Ali, Muzaffar Ali Khan, Muhammad Farooq, Mourad Ben Said, Furhan Iqbal
Summary: In this study, a total of 593 ticks were collected from one-humped camels in two tehsils of Layyah district, Punjab, Pakistan, in order to investigate their diversity, prevalence, and distribution. The results showed that camels located in Tehsil Choubara had a significantly higher tick infestation rate compared to camels from Tehsil Layyah. It was also observed that older camels were more prone to tick infestation. Hyalomma and Rhipicephalus were the two genera identified, with Hyalomma genus being the most prevalent.
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Muhammad Sheraz, Fakhur Ud Din, Muhammad Saad Un Nabi, Sana Rao, Bisma Shafiq, Ghulam Murtaza, Muhammad Farooq, Furhan Iqbal
Summary: The study investigated the genotype and allelic frequency of SNP at 222 G > A in HSP70 and at ex6-7390T22G in the HSP90 gene in 204 sheep from Punjab. The tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) revealed significant genotype frequency variation in the HSP70 gene, while the allelic frequency remained unaffected. The study suggests the genotypic screening of sheep before selection as breeders to improve the profitability and sustainability of animal production systems in Pakistan.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ambar Rafiq, Gul Zareen, Sana Akbar, Muhammad Asif, Nadeem Ahmed, Nashra Bibi, Ume Habiba, Asmat Ullah Khan, Rehan Sadiq Shaikh, Furhan Iqbal
Summary: The aim of this study was to report the genotype and allelic frequency at rs438228855 in SLC35A3 receptor gene and its association with complex vertebral malformation (CMV) in enrolled Pakistani cattle. The results showed non-significant variations in allelic and genotype frequency at rs438228855 among the three cattle breeds. The heterozygous (GT) genotype was most abundant, followed by the wild type (GG) genotype, and the mutant genotype (TT) was not observed. The study also found significant differences in various hematological parameters between the cattle breeds, but no association with the genotype at rs438228855 was observed.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Genetics & Heredity
Kainat Arshad, Uzma Ishfaq, Muhammad Asif, Atif Akbar, Kehkashan Fatima Pitafi, Muhammad Rehan Mulghani, Uzman Shaheen, Suleman Saeed, Muhammad Arif, Ahsan Bashir, Muhammad Farooq, Alan Henry Brook, Furhan Iqbal
Summary: This study investigates the genotypes of GSTM1, GSTT1, and SNP rs1695 in GSTP1 genes and their associations with the incidence of chronic periodontal disease (CP). The results show that the absence of GSTM1, the presence of GSTT1, and the presence of the mutant allele (G) at rs1695 in GSTP1 are significantly associated with CP.
Article
Medicine, Research & Experimental
Ao Ma, Jianteng Zhou, Haider Ali, Tanveer Abbas, Imtiaz Ali, Zubair Muhammad, Sobia Dil, Jing Chen, Xiongheng Huang, Hui Ma, Daren Zhao, Beibei Zhang, Yuanwei Zhang, Wasim Shah, Basit Shah, Ghulam Murtaza, Furhan Iqbal, Muzammil Ahmad Khan, Asad Khan, Qing Li, Bo Xu, Limin Wu, Huan Zhang, Qinghua Shi
Summary: This study identified two mutations in the CFAP57 gene associated with MMAF, and further research revealed that these mutations disrupted the assembly of inner dynein arms in sperm flagella, leading to male infertility.
Article
Public, Environmental & Occupational Health
Asia Taqadus, Chien-Chun Chiou, Itzel Amaro-Estrada, Muhammad Asif, Nasreen Nasreen, Ghafoor Ahmad, Javaid Iqbal, Muhammad Ali, Adil Khan, Furhan Iqbal, Chien-Chin Chen
Summary: The present study aims to report the molecular prevalence of A. ovis in 1200 asymptomatic goats from four districts of Punjab, Pakistan. The results showed that 184 out of 1200 (15%) goat blood samples were infected with A. ovis. Risk factors associated with the prevalence of A. ovis and phylogeny of the bacterium were also documented. This study provides important insights for developing integrated control policies against A. ovis.
VECTOR-BORNE AND ZOONOTIC DISEASES
(2023)
Article
Zoology
Shahid Iqbal, Zulha Javed, Mushtaq Hussain Zahid, Qurat ul Ane Gillani, Furhan Iqbal
Summary: This study investigated the effects of ethanolic extracts of Pongamia pinnata (L.) Pierre leaves and Eucalyptus camaldulensis Dehnh leaves on hematological parameters and biochemical components of blood serum in male albino mice. The results showed that the P. pinnata leaf extract significantly increased cholesterol and triglyceride levels, while the E. camaldulensis leaf extract significantly reduced white blood cells, platelets, and alanine aminotransferase, and increased mean corpuscular hemoglobin levels. Both extracts showed a potential to disturb the blood biochemistry of healthy male albino mice, with more pronounced effects observed with the E. camaldulensis leaf extract.
PAKISTAN JOURNAL OF ZOOLOGY
(2023)