Article
Biochemistry & Molecular Biology
Yue Chen, Meng-huan Wang, Jin-yi Wu, Jian-yun Zhu, Chun-feng Xie, Xiao-ting Li, Jie-shu Wu, Shan-shan Geng, Ya-dong Li, Hong-yu Han, Cai-yun Zhong
Summary: Cancer stem cells (CSCs) are crucial in cancer and can be targeted by the natural compound SFN through the regulation of delta Np63α expression and the activation of core CSCs gene transcription.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Cell Biology
Clayton B. Marshall, J. Scott Beeler, Brian D. Lehmann, Paula Gonzalez-Ericsson, Violeta Sanchez, Melinda E. Sanders, Kelli L. Boyd, Jennifer A. Pietenpol
Summary: The study revealed significant correlation in expression of p73 and p63 isoforms across human tissues, with nuclear co-expression observed in the majority of cells. The alpha-isoforms of TP73 and TP63 were found to be the predominant isoform expressed in nearly all tissues, with the identification of a previously unreported p73 mRNA product.
CELL DEATH & DISEASE
(2021)
Editorial Material
Genetics & Heredity
Hartwig Visser, Adam D. Thomas
Summary: DNA damage-induced miRNAs are important in the DNA damage response, influencing cell fate. However, it is still unclear who is in charge in this process.
TRENDS IN GENETICS
(2021)
Article
Cell Biology
Christopher E. Eyermann, Jinyu Li, Evguenia M. Alexandrova
Summary: Pregnancy is known to reduce the risk of breast cancer, but it may promote HER2+ BC. Studies have shown that pregnancy can increase the incidence of HER2+ BC in mice models, possibly linked to the tumor-initiating properties of PIMECs. The gene p63 plays a complex role in maintaining PIMEC quantity while suppressing their tumorigenic capacity.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Lifeng Feng, Muchun Li, Xinyang Hu, Yiling Li, Liyuan Zhu, Miaoqin Chen, Qi Wei, Wenxia Xu, Qiyin Zhou, Weikai Wang, Dingwei Chen, Xian Wang, Hongchuan Jin
Summary: Chemoresistance, previously seen as a stable outcome resulting from genetic changes, can also be unstable and reversible due to non-genetic alterations. The downregulation of ATF4 protein expression is associated with chemoresistance in gastric cancer cells, and its stabilization through inhibition of degradation can overcome resistance. ATF4 expression plays a crucial role in dynamic drug resistance, and interventions targeting its degradation pathway show promise in overcoming chemoresistance.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Aileen Roth, Annabelle Sander, Marleen Silke Oswald, Fabian Gaertner, Uwe Knippschild, Joachim Bischof
Summary: This study analyzed the phosphorylation of tau proteins by CK1 delta in Alzheimer's disease. Through in vitro experiments and mass spectrometry, ten potential phosphorylation sites were identified, with five of them associated with Alzheimer's disease. Further analysis confirmed the specific phosphorylation sites and demonstrated the potential role of CK1 delta in tau aggregation. These findings highlight the potential of CK1 delta as a promising target for Alzheimer's disease treatment.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Oncology
Ken-ichi Kudo, Naohiro Tsuyama, Kento Nagata, Tatsuhiko Imaoka, Daisuke Iizuka, Misaki Sugai-Takahashi, Moe Muramatsu, Akira Sakai
Summary: The study demonstrated that Delta Np63 alpha can inhibit p53-related radiation-induced DDR, leading to genomic instability in epithelial stem cells.
RADIATION ONCOLOGY
(2022)
Article
Oncology
Eid S. Alshammari, Amjad A. Aljagthmi, Andrew J. Stacy, Mike Bottomley, H. Nicholas Shamma, Madhavi P. Kadakia, Weiwen Long
Summary: Np63 alpha is upregulated in NMSCs, while ERK3 is significantly upregulated in NMSCs, with a strong positive correlation between Np63 alpha and ERK3 in normal skin and NMSC specimens. Np63 alpha positively regulates ERK3 transcript and protein levels in A431 and HaCaT skin cells, which leads to upregulation of ERK3 expression and correlation with Np63 alpha in NMSCs. Additionally, ERK3 inhibits Rac1 G-protein phosphorylation and filopodia formation in A431 skin SCC cells.
Article
Endocrinology & Metabolism
Hongzhou Guo, Di Zhang, Yewen Zhou, Longjie Sun, Changping Li, Xuan Luo, Jiali Liu, Sheng Cui
Summary: Our study showed that CK1 alpha is expressed in both germ cells and somatic cells of mouse testes and plays a crucial role in regulating testosterone synthesis and male reproduction. We found that conditional disruption of CK1 alpha in Leydig cells significantly reduced testosterone levels, affected testis development, sperm motility, and sperm morphology, and caused subfertility. Furthermore, we discovered that luteinizing hormone can upregulate CK1 alpha through a specific signaling pathway and that CK1 alpha can interact with and phosphorylate EGFR, leading to the activation of ERK1/2 and the promotion of testosterone synthesis.
Article
Medicine, Research & Experimental
Vitaly Ievlev, Thomas J. Lynch, Kyle W. Freischlag, Caitlyn B. Cries, Anit Shah, Albert C. Pai, Bethany A. Ahlers, Soo Park, John F. Engelhardt, Kalpaj R. Parekh
Summary: Keratin expression changes dynamically in airway basal cells (BCs) after injury, and the switch from keratin15 (Krt15) to keratin14 (Krt14) is associated with decreased BC clonogenicity. In this study, the roles of Krt14 and Krt15 were investigated using Crispr-KO, and it was found that Krt14-KO and Krt15-KO produced contrasting phenotypes. Krt14-KO enhanced clonogenicity but impaired differentiation, while Krt15-KO had normal differentiation but impaired clonogenicity. This work demonstrates the functional regulation of BC behavior by Krt14 and Krt15, which is relevant in chronic diseases like bronchiolitis obliterans (BO).
Article
Oncology
Sonja J. Gill, Paul W. G. Wijnhoven, Jacqueline H. L. Fok, Rebecca L. Lloyd, Jonathan Cairns, Joshua Armenia, Jenni Nikkila, Alan Lau, Christopher J. Bakkenist, Susan M. Galbraith, Conchita Vens, Mark J. O'Connor
Summary: Radiopotentiation profiling of DNA damage response inhibitors (DDRi) revealed lower effective concentrations in radiotherapy combinations, with preferential sensitization of p53-deficient cells. Olaparib, ceralasertib, and adavosertib showed moderate increases in radiotherapy dose enhancement with increasing inhibitor concentration, while small increases in AZD0156 and KU-60648 resulted in steep increases in dose enhancement. Multiple concentration testing demonstrated a relationship between drug concentration and radiotherapy effect, providing valuable insights for dose-escalation strategies in clinical trials.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Huimin Chen, Ke Hu, Ying Xie, Yucheng Qi, Wenjuan Li, Yaohui He, Shijie Fan, Wen Liu, Chenghua Li
Summary: CDK1 is identified as a novel regulator of Delta Np63 alpha, which modulates EMT and cell migration in HNSCCs. Overexpression of CDK1 is negatively correlated with metastasis and overall survival in HNSCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuemeng Wang, Qile Zhu, Shiya Guo, Juan Ao, Wenhua Zhang, Junjie Fei, Shuhan Yu, Mengmeng Niu, Yujun Zhang, Michael Y. Y. Sherman, Zhi-Xiong Jim Xiao, Yong Yi
Summary: This study reveals that heat shock factor 1 (HSF1) promotes the transcription of delta Np63 alpha via FOXO3a, leading to increased expression of cyclin-dependent kinase 4 and promoting head and neck squamous cell carcinoma (HNSCC) tumor growth.
Article
Cell Biology
Pengyi Yan, Zixuan Li, Junhao Xiong, Zilong Geng, Weiting Wei, Yan Zhang, Gengze Wu, Tao Zhuang, Xiaoyu Tian, Zhijie Liu, Junling Liu, Kun Sun, Fengyuan Chen, Yuzhen Zhang, Chunyu Zeng, Yu Huang, Bing Zhang
Summary: In this study, LARP7 is identified as an aging antagonist, and the ATM-LARP7-SIRT1-p53/p65 pathway plays a crucial role in DNA damage response-mediated cellular senescence and atherosclerosis.
Article
Biochemistry & Molecular Biology
Yuichiro Kanno, Nao Saito, Naoya Yamashita, Kazuki Ota, Ryota Shizu, Takuomi Hosaka, Kiyomitsu Nemoto, Kouichi Yoshinari
Summary: This study found that HER2-activated AHR can upregulate the expression of Delta Np63, thus contributing to the maintenance of breast cancer stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)