期刊
CELL CYCLE
卷 9, 期 5, 页码 1016-1020出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cc.9.5.10953
关键词
retinoblastoma protein; E2F transcription factor; cyclin dependent kinase inhibitor; drosophila; DEAD-box protein
类别
资金
- American Heart Association [0815661G]
- National Institutes of Health [GM079774]
The retinoblastoma protein (pRB) negatively regulates cell proliferation by limiting the activity of the family of E2F transcription factors. In Drosophila, mutation of the DEAD-box helicase belle (bel) relieves an E2F/pRB induced G(1) cell cycle arrest; however, the mechanism of this rescue is unknown. Here, we show that the level of the cyclin-dependent kinase inhibitor Dacapo (Dap), homolog of mammalian p21/p27, is strongly reduced both in bel mutant cells in vivo and in tissue culture cells depleted of Bel by RNA interference. Interestingly, the loss of bel also partially alleviates an ectopically induced G(1) cell cycle arrest. Additionally, we show that Bel undergoes nucleocytoplasmic shuttling. Thus, inactivation of bel renders cells less sensitive to several anti-proliferative signals inducing G(1) arrest.
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