Article
Chemistry, Analytical
Bo Hu, Qin Zhang, Xiaonan Gao, Kehua Xu, Bo Tang
Summary: By designing a multicolor fluorescent nanoprobe Cas-NP, the activation of caspases-1/3/4 during pyroptosis can be accurately monitored, revealing the pathways of pyroptosis under different stimuli. Through studying the pyroptosis process in cancer cells, it was first discovered that LPS stimulation can induce caspase-3 activation, and the activation sequence of caspases-1/3/4 was verified.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Negar Ghorbani, Roham Yaghubi, Jamshid Davoodi, Sara Pahlavan
Summary: Caspases are important proteases involved in cellular events such as apoptosis and inflammation. They also have non-apoptotic pro-survival functions, which are crucial in processes like cell proliferation, differentiation, and migration, as well as tumor metastasis and chemotherapy resistance. Understanding the mechanisms that regulate caspase activities is important for both mechanistic and clinical purposes. Additionally, the functions of apoptotic caspases are affected by various post-translational modifications.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Peipei Zhang, Yifei Liu, Lichen Hu, Kai Huang, Mao Hong, Yuze Wang, Xinrui Fan, Richard J. Ulevitch, Jiahuai Han
Summary: The overactivation of the inflammasome can lead to host death due to cell death. Blocking multiple death pathways can prevent mice death caused by NLRC4 inflammasome overactivation and reduce the lethality of infection-related diseases, such as those caused by Salmonella infection.
Article
Cell Biology
Bowen Zhou, Derek W. Abbott
Summary: Gasdermin E (GSDME) functions as an independent conduit for interleukin-1b (IL-1b) release in cellular inflammasome activation, regardless of its cell death ability. Our study reveals that both NLRP3 and NLRP1 inflammasomes depend on both gasdermins for IL-1b processing and release, apart from their ability to induce cell lysis.
Article
Cell Biology
Minsuk Kwak, Kaden M. Southard, Woon Ryoung Kim, Annie Lin, Nam Hyeong Kim, Ramu Gopalappa, Hyun Jung Lee, Minji An, Seo Hyun Choi, Yunmin Jung, Kunwoo Noh, Justin Farlow, Anastasios Georgakopoulos, Nikolaos K. Robakis, Min K. Kang, Matthew L. Kutys, Daeha Seo, Hyongbum Henry Kim, Yong Ho Kim, Jinwoo Cheon, Zev J. Gartner, Young-wook Jun
Summary: Adherens junctions (AJs) play a crucial role in organizing proteolytic hotspots and microdomains for the processing of transmembrane proteins such as Notch. Membrane microdomains within AJs coordinate regulated intramembrane proteolysis (RIP), while those outside AJs mediate the engagement of Notch ligand-receptor interactions. This mechanism directs the differentiation of neural progenitor cells and regulates the proteolysis of other cell-surface receptors.
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
Lingyu Pan, Yan Chen, Yeke Jiang, Yehong Sun, Yanquan Han, Yongzhong Wang
Summary: The study demonstrates that Yanghe Pingchuan granules can significantly improve the inflammatory response in a rat model of bronchial asthma by blocking the TLR4/NF-κB signaling pathway and inhibiting pyroptosis factor expression.
MEDIATORS OF INFLAMMATION
(2022)
Article
Immunology
Delphine Bonhomme, Veronica Hernandez-Trejo, Stylianos Papadopoulos, Remi Pigache, Martine Fanton d'Andon, Ahmed Outlioua, Ivo G. Boneca, Catherine Werts
Summary: Leptospira interrogans is capable of infecting all vertebrates and causing leptospirosis, but they do not trigger cell death in macrophages and escape pyroptosis and apoptosis. Instead, they induce mild IL-1b secretion through nonlytic caspase 8-dependent gasdermin D pore formation. Pathogenic L. interrogans prevent caspase 11 dimerization and gasdermin D cleavage, thus escaping massive cell death. This novel mechanism allows leptospires to evade inflammation and potentially contribute to their stealthiness.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomoya Fukuoka, Kenta Moriwaki, Shinji Takamatsu, Jumpei Kondo, Miki Tanaka-Okamoto, Azusa Tomioka, Manami Semba, Sachiko Komazawa-Sakon, Yoshihiro Kamada, Hiroyuki Kaji, Yasuhide Miyamoto, Masahiro Inoue, Kazuhiko Bessho, Yoko Miyoshi, Keiichi Ozono, Hiroyasu Nakano, Eiji Miyoshi
Summary: The study reveals that Lewis glycans positively regulate TRAIL-induced cell death, providing important insights into TRAIL sensitivity. Moreover, the expression of type I Lewis glycans is positively correlated with TRAIL sensitivity in colon cancer cell lines and patient-derived cancer organoids.
Review
Cell Biology
Valeria Manganelli, Agostina Longo, Vincenzo Mattei, Serena Recalchi, Gloria Riitano, Daniela Caissutti, Antonella Capozzi, Maurizio Sorice, Roberta Misasi, Tina Garofalo
Summary: ERLINs are a family of proteins involved in ER-associated protein degradation and regulation of lipid metabolism. They have been traditionally considered markers of ER lipid raft-like microdomains but have also been found in mitochondria-associated endoplasmic reticulum membranes (MAMs) under pathophysiological conditions, influencing cell fate by regulating processes such as survival and death. Their alteration can significantly impact the pathogenesis of various human diseases.
Editorial Material
Biochemistry & Molecular Biology
John E. Ladbury, Chi-Chuan Lin, Kin Man Suen
Summary: Signal transduction is a numbers game, requiring sufficient engagement of cell surface receptors and effector proteins to ensure functional signaling against a background of redundancy. Intracellular liquid-liquid phase separation at the plasma membrane provides a mechanism to weight the probabilistic nature of signaling in favor of the required cellular outcome and mutual exclusivity in signal initiation.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Microbiology
Li-Yu Chen, Lee-Wen Chen, Chien-Hui Hung, Chun-Liang Lin, Shie-Shan Wang, Pey-Jium Chang
Summary: This study reveals that Sp3 protein is proteolytically cleaved during the viral lytic cycle of KSHV, and identifies 12 caspase cleavage sites in Sp3. Despite the cleavage, the resulting C-terminal fragments of Sp3 retain the ability to synergistically activate specific gene promoters with ORF50.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Hin Chu, Yuxin Hou, Dong Yang, Lei Wen, Huiping Shuai, Chaemin Yoon, Jialu Shi, Yue Chai, Terrence Tsz-Tai Yuen, Bingjie Hu, Cun Li, Xiaoyu Zhao, Yixin Wang, Xiner Huang, Kin Shing Lee, Cuiting Luo, Jian-Piao Cai, Vincent Kwok-Man Poon, Chris Chung-Sing Chan, Anna Jinxia Zhang, Shuofeng Yuan, Ko-Yung Sit, Dominic Chi-Chung Foo, Wing-Kuk Au, Kenneth Kak-Yuen Wong, Jie Zhou, Kin-Hang Kok, Dong-Yan Jin, Jasper Fuk-Woo Chan, Kwok-Yung Yuen
Summary: This study reveals that caspase-6, a protease in the apoptosis cascade, plays an important role in efficient replication of coronaviruses. It is shown that caspase-6 cleaves coronavirus nucleocapsid proteins to generate fragments that act as interferon antagonists, thereby facilitating virus replication. Inhibition of caspase-6 attenuates lung pathology and weight loss in infected animals, highlighting the potential of targeting caspase-6 as a therapeutic strategy for coronavirus infections.
Article
Cell Biology
Shengyu Li, Lifeng Feng, Guangru Li, Ruiqing Liu, Changzhen Ma, Lin Wang, Aijiao Gao, Chang Liu, Yujie Cui, Zecheng Jiang, Yuhang Xie, Qiang Wu, Xia Wang, Liang Yang, Zhi Qi, Yanna Shen
Summary: Diabetic nephropathy (DN) is a serious complication of diabetes that significantly increases morbidity and mortality. This study found that the GSDME-dependent pyroptosis signaling pathway plays a crucial role in DN, providing important insights for potential therapeutic strategies.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Zirui Liu, Yiquan Li, Yilong Zhu, Nan Li, Wenjie Li, Chao Shang, Gaojie Song, Shanzhi Li, Jianan Cong, Tingyu Li, Zhiru Xiu, Jing Lu, Chenchen Ge, Xia Yang, Yaru Li, Lili Sun, Xiao Li, Ningyi Jin
Summary: Apoptin inhibits the viability of HCT116 cells through inducing pyroptosis mediated by GSDME, as well as enhancing intracellular ROS production. The findings in nude mice xenografted with HCT116 cells demonstrate that Apoptin induces pyroptosis and significantly inhibits tumor growth. These discoveries provide a new strategy for colorectal cancer therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Binlong Chen, Yue Yan, Ye Yang, Guang Cao, Xiao Wang, Yaoqi Wang, Fangjie Wan, Qingqing Yin, Zenghui Wang, Yunfei Li, Letong Wang, Bo Xu, Fuping You, Qiang Zhang, Yiguang Wang
Summary: The authors in this paper engineer a library of ultra-pH-sensitive nanophotosensitizers that can target specific endocytic organelles and induce pyroptotic cancer cell death in a controlled manner. By spatiotemporally targeting distinct stages of endosomal maturation, they achieve tunable cellular pyroptosis. The nanotuner platform demonstrates enhanced antitumor efficacy and minimized systemic side effects in various gasdermin-E-positive human cancers.
NATURE NANOTECHNOLOGY
(2022)
Editorial Material
Oncology
Chantal Thibert, Christine Perret, Marc Billaud
Article
Developmental Biology
Sophie E. Creuzet, Jean P. Viallet, Maya Ghawitian, Sakina Torch, Jacques Thelu, Moussab Alrajeh, Anca G. Radu, Daniel Bouvard, Floriane Costagliola, Mallys Le Borgne, Karine Buchet-Poyau, Nicolas Aznar, Sylvie Buschlen, Hiroshi Hosoya, Chantal Thibert, Marc Billaud
DEVELOPMENTAL BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Flavie Strappazzon, Sakina Torch, Christine Chatellard-Causse, Anne Petiot, Chantal Thibert, Beatrice Blot, Jean-Marc Verna, Remy Sadoul
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2010)
Article
Biochemistry & Molecular Biology
F. Mille, F. Llambi, C. Guix, C. Delloye-Bourgeois, C. Guenebeaud, S. Castro-Obregon, D. E. Bredesen, C. Thibert, P. Mehlen
CELL DEATH AND DIFFERENTIATION
(2009)
Article
Developmental Biology
Mailys Le Borgne, Nicolas Chartier, Karine Buchet-Poyau, Olivier Destaing, Eva Faurobert, Chantal Thibert, Jean-Pierre Rouault, Julien Courchet, Didier Negre, Daniel Bouvard, Corinne Albiges-Rizo, Sophie Rousseaux, Saadi Khochbin, Dominique Segretain, Pascale Crepieux, Florian Guillou, Philippe Durand, Marie-Helene Perrard, Marc Billaud
Review
Medicine, Research & Experimental
Martine Cordier-Bussat, Chantal Thibert, Pierre Sujobert, Laurent Genestier, Eric Fontaine, Marc Billaud
M S-MEDECINE SCIENCES
(2018)
Article
Cell Biology
Frederic Mille, Chantal Thibert, Joanna Fombonne, Nicolas Rama, Catherine Guix, Hideki Hayashi, Veronique Corset, John C. Reed, Patrick Mehlen
NATURE CELL BIOLOGY
(2009)
Article
Multidisciplinary Sciences
Joanna Fombonne, Pierre-Antoine Bissey, Catherine Guix, Remy Sadoul, Chantal Thibert, Patrick Mehlen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Anca G. Radu, Sakina Torch, Florence Fauvelle, Karin Pernet-Gallay, Anthony Lucas, Renaud Blervaque, Veronique Delmas, Uwe Schlattner, Laurence Lafanechere, Pierre Hainaut, Nicolas Tricaud, Veronique Pingault, Nadege Bondurand, Nabeel Bardeesy, Lionel Larue, Chantal Thibert, Marc Bilaud
Article
Biochemistry & Molecular Biology
Bruno Pereira, Ana L. Amaral, Alexandre Dias, Nuno Mendes, Vanesa Muncan, Ana R. Silva, Chantal Thibert, Anca G. Radu, Leonor David, Valdemar Maximo, Gijs R. van den Brink, Marc Billaud, Raquel Almeida
Review
Anatomy & Morphology
Chantal Thibert, Anthony Lucas, Marc Billaud, Sakina Torch, Marie Mevel-Aliset, Jordan Allard
Summary: Neural crest cells are embryonic cells that migrate long distances to reach target organs and give rise to various derivatives. Recent studies have highlighted the crucial role of the metabolic kinase LKB1 in the establishment and maintenance of neural crest derivatives. The underlying molecular mechanisms involve the AMPK-mTOR signaling pathway, offering potential therapeutic applications for neural crest disorders.
DEVELOPMENTAL DYNAMICS
(2023)
Meeting Abstract
Cell Biology
A. G. Radu, S. Torch, F. Fauvelle, P. Hainaut, L. Larue, C. Thibert, M. Billaud
MOLECULAR BIOLOGY OF THE CELL
(2017)
Meeting Abstract
Oncology
Pierre-Antoine Bissey, Joanna Fombonne, Catherine Guix, Chantal Thibert, Remy Sadoul, Patrick Mehlen
BULLETIN DU CANCER
(2011)
Article
Neurosciences
P Mehlen, F Mille, C Thibert
JOURNAL OF NEUROBIOLOGY
(2005)