Article
Cell Biology
Junjie Huang, Zhuobi Liang, Cuirong Guan, Shasha Hua, Kai Jiang
Summary: WDR62 acts as an adaptor protein between TPX2/Aurora A and katanin to regulate spindle dynamics by recruiting katanin to the spindle pole. It shows preference for curved segments of dynamic GDP-MTs and its MT-binding affinity is autoinhibited through JNK phosphorylation-induced intramolecular interaction.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Ziqi Yan, Qiong Shi, Xumei Liu, Jinghua Li, Vidula Ahire, Shenqiu Zhang, Jing Zhang, Dun Yang, Thaddeus D. Allen
Summary: Plants are a valuable source of bioactive compounds, and in this study, two phytochemicals, Corynoline and Acetylcorynoline, were found to have inhibitory effects on cancer cells by inducing mitotic arrest and polyploidy. The mechanism of action involves centrosome amplification and declustering, leading to the formation of multi-polar spindles. These compounds inhibited the viability of various human cancer cell lines and could be potential prototypes for the development of more potent and selective analogs in the future.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biochemistry & Molecular Biology
Mrunal Jadhav, Kaksha Sankhe, Richie R. Bhandare, Zehra Edis, Samir Haj Bloukh, Tabassum Asif Khan
Summary: Recent decades have seen significant progress in the discovery of anticancer drugs containing pyrimidine structures, which exhibit diverse mechanisms of action, including inhibition of protein kinases. Literature and patent analysis reveal extensive research and application of pyrimidine derivatives in the field of anticancer drugs.
Review
Pharmacology & Pharmacy
Styliani Iliaki, Rudi Beyaert, Inna S. Afonina
Summary: PLK1 is a Ser/Thr kinase that plays crucial roles in cell cycle regulation, DNA damage response, apoptosis, and cancer progression. Overexpression of PLK1 is associated with poor prognosis in cancer, making it an attractive therapeutic target.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biology
Italia Anna Asteriti, Federica Polverino, Venturina Stagni, Valentina Sterbini, Camilla Ascanelli, Francesco Davide Naso, Anna Mastrangelo, Alessandro Rosa, Alessandro Paiardini, Catherine Lindon, Giulia Guarguaglini
Summary: AurkA kinase is frequently overexpressed in tumors and its nuclear localization in interphase is correlated with its oncogenic potential. The mechanisms leading to AurkA nuclear accumulation are poorly explored, but it is influenced by the cell cycle phase and nuclear export, and not by its kinase activity. Co-overexpression of AURKA and TPX2 or impairment of proteasome activity can induce AurkA nuclear accumulation.
LIFE SCIENCE ALLIANCE
(2023)
Article
Developmental Biology
Mansour Aboelenain, Karen Schindler
Summary: This study demonstrates that AURKB negatively regulates CPEB1-dependent translation through modulation of AURKA activity, which is crucial for generating euploid eggs.
Article
Cell Biology
Corbin R. Azucenas, T. Alex Ruwe, John P. Bonamer, Bo Qiao, Tomas Ganz, Mika Jormakka, Elizabeta Nemeth, Bryan Mackenzie
Summary: Fpn is a protein expressed in the plasma membrane of macrophages, enterocytes, and hepatocytes, which mediates the transfer of cellular iron into the blood plasma. Research has shown that human Fpn does not transport manganese, but mouse Fpn may be involved in manganese metabolism. Comparative analysis of mouse and human Fpn revealed that they share identical properties in terms of substrate profile, calcium dependence, optimal pH, and hepcidin sensitivity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jin-Gyeong Park, Hanul Jeon, Sangchul Shin, Chiman Song, Hyomin Lee, Nak-Kyoon Kim, Eunice EunKyeong Kim, Kwang Yeon Hwang, Bong-Jin Lee, In-Gyun Lee
Summary: This study identified a conserved region of CEP192 that interacts with AURKA and revealed the structural basis for CEP192-mediated regulation of AURKA at the centrosome. This regulation is distinct from TPX2-mediated regulation on the spindle microtubule.
Article
Cell Biology
Stylianos Didaskalou, Christos Efstathiou, Sotirios Galtsidis, Ilona Kesisova, Aliaksandr Halavatyi, Tountzai Elmali, Avgi Tsolou, Andreas Girod, Maria Koffa
Summary: Accurate chromosome segregation requires proper formation of the mitotic spindle, and the localization of HURP protein in close proximity to the chromosomes is crucial for this process. In this study, we investigated the impact of microtubule flux and phosphorylation of HURP at Ser627 on its dynamics using photoactivation and FRAP experiments. Additionally, we identified the interactions of HURP with various proteins during mitosis through immunoprecipitation assays. Our findings show that phosphorylation of HURP at Ser627 regulates its interaction with these partners, and HURP participates in at least two distinct complexes during metaphase to ensure its proper localization and promote the bundling and stabilization of kinetochore fibers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Manjuan Zhang, Fengrui Yang, Wenwen Wang, Najdat Zohbi, Xiwei Wang, Dongmei Wang, Xiaoxuan Zhuang, Zhen Dou, Dan Liu, Xiaoyu Song, Hadiyah-Nicole Green, Xing Liu, Xuebiao Yao
Summary: The dynamic interactions between SKAP and Aurora B via phase separation play a crucial role in orchestrating accurate interaction between the kinetochore and spindle microtubules during mitosis, ensuring faithful chromosome segregation and alignment. This novel mechanism forms a dynamic pool of Aurora B activity that facilitates the conversion of kinetochore-microtubule attachments from lateral to end-on, ultimately leading to successful cell division.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Plant Sciences
Jihee Hong, Dasom Gwon, Chang-Young Jang
Summary: Ginsenoside Rg1 reduces cancer cell proliferation by inhibiting Haspin kinase activity and H3T3ph, leading to decreased levels of Aurora B at the centromere.
JOURNAL OF GINSENG RESEARCH
(2022)
Article
Oncology
Manuela Mancini, Cecilia Monaldi, Sara De Santis, Michela Rondoni, Cristina Papayannidis, Chiara Sartor, Antonio Curti, Samantha Bruno, Michele Cavo, Simona Soverini
Summary: This study aimed to identify new therapeutic targets in the pathogenesis of systemic mastocytosis (SM). The results identified Aurora kinase A and Polo-like kinase 1 as potential targets for SM treatment. Inhibiting these kinases can induce apoptotic cell death in SM cell lines, suggesting it as an attractive therapeutic strategy. Further clinical development of inhibitors for these kinases could improve the outcome of patients with advanced SM.
Article
Medicine, Research & Experimental
Atsushi Yamaguchi, Yuto Mukai, Tomoya Sakuma, Yudai Suganuma, Ayako Furugen, Katsuya Narumi, Masaki Kobayashi
Summary: This study examined the influence of hMCT9 gene variants L93M and T258K on its transport characteristics. The results showed that L93M slightly decreased the transport activity of creatine, while T258K did not affect it. Interestingly, T258K abolished Na+ sensitivity and altered the substrate affinity.
Article
Cell Biology
Eric M. C. Britigan, Jun Wan, Daniel K. Sam, Sarah E. Copeland, Amber L. Lasek, Laura C. F. Hrycyniak, Lei Wang, Anjon Audhya, Mark E. Burkard, Avtar Roopra, Beth A. Weaver
Summary: The increased expression of Aurora B protein reduces its kinase activity and causes defects in mitosis. The complexes of Aurora B and its binding partner INCENP achieve Aurora B activation through autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Alessio Stefani, Geny Piro, Francesco Schietroma, Alessandro Strusi, Emanuele Vita, Simone Fiorani, Diletta Barone, Federico Monaca, Ileana Sparagna, Giustina Valente, Miriam Grazia Ferrara, Ettore D'Argento, Mariantonietta Di Salvatore, Carmine Carbone, Giampaolo Tortora, Emilio Bria
Summary: Lung cancer is categorized into NSCLC and SCLC, with the former having actionable targets for advanced treatment and the latter lacking oncogene-addiction concept. Aurora kinases (AURKs) play a crucial role in cell cycle progression and their overexpression is a common protumorigenic pathway in various cancer types, influencing drug resistance mechanisms.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Julie Bronsard, Gaetan Pascreau, Mohamed Sassi, Tony Mauro, Yoann Augagneur, Brice Felden
SCIENTIFIC REPORTS
(2017)
Article
Cell Biology
Gaetan Pascreau, Mair E. A. Churchill, James L. Maller
Article
Developmental Biology
Gaetan Pascreau, Jean-Guy Delcros, Nathalie Morin, Claude Prigent, Yannick Arlot-Bonnemains
DEVELOPMENTAL BIOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Gaetan Pascreau, Frank Eckerdt, Andrea L. Lewellyn, Claude Prigent, James L. Maller
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Cell Biology
Rebecca L. Ferguson, Gaetan Pascreau, James L. Maller
JOURNAL OF CELL SCIENCE
(2010)
Article
Multidisciplinary Sciences
Gaetan Pascreau, Frank Eckerdt, Mair E. A. Churchill, James L. Maller
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Biochemistry & Molecular Biology
G Pascreau, JG Delcros, JY Cremet, C Prigent, Y Arlot-Bonnemains
JOURNAL OF BIOLOGICAL CHEMISTRY
(2005)
Article
Cell Biology
L Detivaud, G Pascreau, A Karaïskou, HB Osborne, JZ Kubiak
JOURNAL OF CELL SCIENCE
(2003)