Article
Biology
Ellen Phillips, Naseer Ahmad, Li Sun, James Iben, Christopher J. Walkey, Aleksandra Rusin, Tony Yuen, Clifford J. Rosen, Ian M. Willis, Mone Zaidi, Deborah L. Johnson
Summary: MAF1 is a key regulator of RNA polymerase III-mediated transcription and plays a critical role in osteoblast differentiation and bone mass regulation. Deletion of MAF1 resulted in high bone mass phenotype in mice, while overexpression of MAF1 increased bone mass. MAF1 enhanced osteoblast differentiation, but other perturbations of RNA pol III transcription inhibited differentiation.
Article
Genetics & Heredity
Joseph Blayney, James Geary, Ruby Chrisp, Joseph Violet, Liam Barratt, Katherine Paine, Ian A. Graham, Katherine J. Denby, Robert J. White
Summary: Maf1 is a conserved transcription factor that plays a crucial role in growth and stress response. Depletion of Maf1 in Arabidopsis was found to enhance growth without compromising resistance to common challenges.
Article
Cell Biology
Chiaki Noguchi, Lucy Wang, Mihir Shetty, Joshua Chang Mell, Christian Sell, Eishi Noguchi
Summary: The key to longevity assurance lies in the nutrient-sensing mTOR pathway. Maf1 has been identified as a critical downstream regulator of the mTOR pathway, playing a crucial role in extending lifespan through its control of RNA polymerase III-directed transcription. This study highlights the importance of Maf1 in promoting genomic integrity and maintaining normal lifespan under conditions of DNA replication-transcription conflicts.
Article
Biochemistry & Molecular Biology
Christian Sudfeld, Ana Pozo-Rodriguez, Sara A. Manjavacas Diez, Rene H. Wijffels, Maria J. Barbosa, Sarah D'Adamo
Summary: This study reports a novel gene expression system discovered in the oleaginous microalga Nannochloropsis, utilizing the highly efficient transcriptional activity of RNA polymerase I and an internal ribosome entry site for translation. By identifying the nucleolus as a genomic safe harbor for Pol I transcription and constructing transformant strains, consistently strong transgene expression was achieved, providing a powerful tool for genetic and metabolic engineering of microalgae and potential significant contributions to microalgal research.
Article
Plant Sciences
Qiumin Chen, Mengqi Qu, Qinglei Chen, Xiangnan Meng, Haiyan Fan
Summary: The TOR kinase is a conserved regulator of cell growth in yeasts, plants, and mammals. However, there is a lack of large-scale studies on TOR phosphorylation events under environmental stress. In this study, phosphoproteomics analysis of cucumber under P. xanthii attack with TOR inhibitor pretreatment revealed the unique preferences of TOR in phosphorylation response. These findings provide valuable insights into the molecular mechanism of how TOR kinase controls plant growth and stress adaptation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Plant Sciences
Maria Jose Garcia, Macarena Angulo, Carlos Lucena, Rafael Perez-Vicente, Francisco Javier Romera
Summary: To cope with nutrient scarcity, plants employ two main strategies - stopping growing to reduce nutrient demand, and searching for nutrients through physiological and morphological responses in roots. The interconnection between these strategies is not well understood, but recent research suggests that the plant hormone ethylene may play a crucial role in connecting them. The TOR system is known to be a central regulator of growth in response to nutrients, and ethylene activation is involved in many nutrient deficiency responses.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Oncology
Asimina Zisi, Jiri Bartek, Mikael S. Lindstrom
Summary: Cells, especially cancer cells, need to continuously produce ribosomes to sustain their proliferation. Targeting the machinery involved in ribosome biogenesis has shown potential for developing effective cancer therapies. This review provides information on molecules that target ribosome biogenesis and discusses potential avenues for future research.
Article
Medicine, Research & Experimental
Mengmeng Wang, Xiaoman Liu, Yu Wu, Yi Wang, Jiahui Cui, Jing Sun, Ying Bai, Ming-Fei Lang
Summary: miR-122 upregulation reduces infarct size, Maf1 is a direct target of miR-122, and its expression is negatively associated with miR-122 in an ischemic stroke model.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Multidisciplinary Sciences
Emilio Merheb, Min-Hui Cui, Juwen C. DuBois, Craig A. Branch, Maria Gulinello, Bridget Shafit-Zagardo, Robyn D. Moir, Ian M. Willis
Summary: Mice expressing pathogenic mutations in the largest Pol III subunit, Polr3a, show impaired growth, developmental delay, cognitive deficits, and hypomyelination in certain brain regions, resembling some clinical features of patients with Pol III-related leukodystrophy. The disease pathogenesis in the mice involves defects in oligodendrogenesis and myelination processes sensitive to perturbations of Pol III transcription.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Abdallah Alhaj Sulaiman, Reem Ali, Dindial Ramotar
Summary: The rapamycin-resistant mutation in histone H2B R95A weakens the association between H2B and Spt16, leading to the loss of STE5 expression and cell cycle arrest response to rapamycin. Mutation of Spt16 also affects the expression of STE5 and demonstrates sensitivity to rapamycin. These findings suggest that histone H2B Arg95 residue is crucial for recruiting Spt16 and maintaining STE5 expression for cell cycle arrest response to rapamycin.
SCIENTIFIC REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Tomasz W. Turowski, Magdalena Boguta
Summary: RNAPI and RNAPIII are multi-heterogenic protein complexes specialized in transcribing highly abundant non-coding RNAs, more complex than RNAPII synthesizing mRNAs. They share associated subcomplexes formed by specific subunits in yeast and establish an assembly platform co-translationally during the synthesis of the Rpb10 subunit.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Debao Xie, Xiangdong Zhao, Lujie Ma, Le Wang, Pan Li, Hongqian Cheng, Zhaoying Li, Pei Zeng, Jing Zhang, Fanli Zeng
Summary: The proteins Rba50 and Gpn2 play important roles in the assembly of RNA polymerase. Their interaction is crucial for recruiting Rpb2, and the Rba50-Gpn2 complex has a similar role in the assembly of RNAPIII.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Inmaculada Couso, Amanda L. Smythers, Megan M. Ford, James G. Umen, Jose L. Crespo, Leslie M. Hicks
Summary: This study reveals the synergistic role of inositol pyrophosphates and TOR signaling in coordinating cell growth and lipid metabolism in green algae. The inhibitory effect of rapamycin on TOR signaling is more significant in the vip1-1 mutant compared to wild-type, highlighting the independent yet coordinated interaction between InsPs and TOR. Additionally, phosphorylation patterns of photosystem proteins are affected by light stress and other stresses, indicating a novel role for inositol pyrophosphates and TOR signaling in response to environmental cues.
Article
Chemistry, Analytical
Huanxin Xue, Jiaye Lu, Hongwei Yan, Ju Huang, Hai-Bin Luo, Man Shing Wong, Yuqi Gao, Xiaolei Zhang, Lei Guo
Summary: In this study, a novel organic fluorescent probe was developed for rapid detection of lung cancer cells and specific targeting of the nucleolus in the cell. By interfering with rRNA synthesis in the nucleolus, the probe induced cell apoptosis, showing promising anti-cancer effects.
Article
Biology
Heena Puri, Vikas Jindal
Summary: This study utilized RNA interference technique to investigate the importance of the TOR gene in whitefly, and found that it significantly regulates the survival and reproduction of whitefly, suggesting it as a potential target for insect management.
JOURNAL OF BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Yuehua Wei, Cynthia Kenyon
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Oncology
Ying Cai, Yue-Hua Wei
Article
Biology
Wei Jiang, Yuehua Wei, Yong Long, Arthur Owen, Bingying Wang, Xuebing Wu, Shuo Luo, Yongjun Dang, Dengke K. Ma
Article
Oncology
Robert Berahovich, Hua Zhou, Shirley Xu, Yuehua Wei, Jasper Guan, Jian Guan, Hizkia Harto, Shuxiang Fu, Kaihuai Yang, Shuying Zhu, Le Li, Lijun Wu, Vita Golubovskaya
Article
Cell Biology
Congrong Wang, Yinan Zhang, Fengwen Li, Yuehua Wei
Article
Cell Biology
Gaojian Zhou, Chuan Huang, Liu Xing, Le Li, Yan Jiang, Yuehua Wei
Article
Multidisciplinary Sciences
Zhe Zhang, Meirong Bai, Guilherme Oliveira Barbosa, Andrew Chen, Yuehua Wei, Shuo Luo, Xin Wang, Bingying Wang, Tatsuya Tsukui, Hao Li, Dean Sheppard, Thomas B. Kornberg, Dengke K. Ma
Article
Biochemistry & Molecular Biology
Xin Li, Robert Berahovich, Hua Zhou, Xianghong Liu, Feng Li, Shirley Xu, Yuehua Wei, Djamila Ouaret, Walter Bodmer, Lijun Wu, Vita Golubovskaya
FRONTIERS IN BIOSCIENCE-LANDMARK
(2020)