Article
Multidisciplinary Sciences
Ho-Soo Lee, Sunwoo Min, Ye-Eun Jung, Sunyoung Chae, June Heo, Jae-Ho Lee, TaeSoo Kim, Ho-Chul Kang, Makoto Nakanish, Sun-Shin Cha, Hyeseong Cho
Summary: The study reveals that the RSF1-PLK1 axis plays a crucial role in the activation of Aurora B, enhancing its catalytic activity through phosphorylation and promoting chromosome alignment and segregation. This kinase combination is crucial for regulating dynamic microtubule-kinetochore attachment in early mitosis.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Joao Barbosa, Claudio E. Sunkel, Carlos Conde
Summary: This review provides an overview of the molecular strategies that monitor and fine-tune KT-MT attachment formation during mitosis, to ensure accurate segregation of sister chromatids. This process is crucial for safe mitotic progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Bernardo Orr, Filipe De Sousa, Ana Margarida Gomes, Olga Afonso, Luisa T. Ferreira, Ana C. Figueiredo, Helder Maiato
Summary: Aurora B kinase plays a crucial role in human cell division by actively monitoring and correcting frequent chromosome segregation errors to prevent micronuclei formation.
Article
Cell Biology
Divine Mensah Sedzro, Xiao Yuan, McKay Mullen, Umer Ejaz, Tongtong Yang, Xu Liu, Xiaoyu Song, Yun-Chi Tang, Weijun Pan, Peng Zou, Xinjiao Gao, Dongmei Wang, Zhikai Wang, Zhen Dou, Xing Liu, Xuebiao Yao
Summary: The study reveals that CENP-R collaborates with CENP-OPQU to regulate the stability of kinetochore-microtubule attachment and ensure accurate chromosome segregation during mitosis. Phosphorylation of CENP-R affects its localization to the kinetochore, possibly by disrupting its binding with CENP-U.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biology
Qin Li, Yanfang Ma, Fen Chang, Yongjie Xu, Jingcheng Deng, Junyi Duan, Wei Jiang, Qihua He, Luzheng Xu, Lijun Zhong, Genze Shao, Li Li
Summary: In this study, we discovered that Aurora B is activated by K63 ubiquitination during mitosis, and BRISC acts as a specific deubiquitinating enzyme for Aurora B. K63 ubiquitination plays a crucial role in regulating Aurora B activation, providing a potential site for the design of Aurora B-targeting drugs.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shuyu Li, Luis J. Garcia-Rodriguez, Tomoyuki U. Tanaka
Summary: This study reveals the role of Aurora B in regulating the biorientation process in yeast. The localization of Aurora B at different kinetochore sites is found to be crucial for the establishment and maintenance of biorientation. Additionally, the dynamic turnover of Aurora B during early mitosis is found to be associated with error correction and biorientation.
Review
Biochemistry & Molecular Biology
Ayumu Yamamoto
Summary: Accurate chromosome segregation is crucial for cell proliferation and sexual reproduction. While the mechanisms of attachment selection and elimination are not fully understood, recent studies suggest that chromosome oscillation may play a role in the elimination of erroneous attachments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Michael A. Hadders, Susanne M. A. Lens
Summary: The Chromosomal Passenger Complex (CPC) is a crucial protein complex that regulates various processes during nuclear and cytoplasmic division. It is recruited to centromeres and kinetochores in early mitosis to ensure proper connection of duplicated chromosomes to microtubules. During anaphase, the CPC is relocated to the microtubule overlaps and equatorial cortex, requiring direct interactions with a kinesin-6 family member and inactivation of cyclin B-CDK1.
TRENDS IN CELL BIOLOGY
(2022)
Review
Oncology
Kenji Iemura, Yujiro Yoshizaki, Kinue Kuniyasu, Kozo Tanaka
Summary: Chromosomal instability (CIN) is commonly observed in cancer cells, associated with tumor progression and poor prognosis. Insufficient correction of erroneous kinetochore-microtubule attachments plays pivotal roles in CIN, with chromosome oscillation promoting error correction by phosphorylating Hec1 through Aurora A kinase. Understanding the link between attenuated chromosome oscillation and CIN in cancer cells may provide potential targets for cancer therapy.
Article
Biochemistry & Molecular Biology
Harinath Doodhi, Tomoyuki U. Tanaka
Summary: This article discusses recent findings in budding yeast regarding the molecular mechanisms involved in the error correction process during chromosome segregation in mitosis. The study reveals that Aurora B kinase plays a crucial role in regulating the exchange and cessation of kinetochore-microtubule interactions to resolve aberrant interactions, ultimately ensuring proper chromosome biorientation.
Article
Multidisciplinary Sciences
Jin-Gyeong Park, Hanul Jeon, Sangchul Shin, Chiman Song, Hyomin Lee, Nak-Kyoon Kim, Eunice EunKyeong Kim, Kwang Yeon Hwang, Bong-Jin Lee, In-Gyun Lee
Summary: This study identified a conserved region of CEP192 that interacts with AURKA and revealed the structural basis for CEP192-mediated regulation of AURKA at the centrosome. This regulation is distinct from TPX2-mediated regulation on the spindle microtubule.
Article
Biology
Krystal Timon Perez, Jacopo Scrofani, Isabelle Vernos
Summary: During mitosis, coordination of different microtubule nucleation pathways is essential for bipolar spindle assembly, and phosphorylation plays a crucial role in this process.
Review
Biochemistry & Molecular Biology
J. Priyanga, Gunjan Guha, Dipita Bhakta-Guha
Summary: Centrosome dysregulation is a significant factor in the onset and progression of cancer cells, with the manipulation of centrosome homeostasis being crucial for avoiding centrosome clustering in cancer cells. Microtubule motors play a critical role in maintaining the integrity of centrosomes, and understanding their mechanisms can lead to the development of therapeutic targets for diverse cancers.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Biochemistry & Molecular Biology
Aurelien Courtois, Shuhei Yoshida, Osamu Takenouchi, Kohei Asai, Tomoya S. Kitajima
Summary: This study demonstrates the importance of stable kinetochore-microtubule attachments in confining MTOC positions at spindle poles and limiting spindle elongation in acentriolar spindles. The gradual MTOC sorting process in the metaphase spindle is crucial for maintaining spindle integrity. Disruption of stable kinetochore-microtubule attachments leads to failure in MTOC confinement and spindle elongation termination.
Article
Cell Biology
Marco Novais-Cruz, Antonio Pombinho, Mafalda Sousa, Andre F. Maia, Helder Maiato, Cristina Ferras
Summary: DNA damage in mitosis leads to misalignment of chromosomes and causes mitotic delay and micronuclei formation. Chromokinesin-mediated missegregation of polar chromosomes contributes to genomic instability induced by therapeutic DNA damage regimens in cancer cells.
MOLECULAR BIOLOGY OF THE CELL
(2023)