Article
Biochemistry & Molecular Biology
Mahara Valverde, Aaron Sanchez-Brito
Summary: Early inflammatory environments can impact adipocyte function, leading to sustained activation of DNA damage response, triggering adipocyte dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Beata P. Plitta-Michalak, Alice A. Ramos, Piotr Pupel, Marcin Michalak
Summary: The high-throughput comet assay is a sensitive tool for monitoring DNA integrity changes and assessing the viability status of plant germplasm.
Article
Food Science & Technology
Julia Buchmueller, Anne-Margarethe Enge, Antonia Peters, Johanna Ebmeyer, Jan-Heiner Kuepper, Bernd Schaefer, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study investigated the genotoxic profile of varying degrees of esterification of unsaturated pyrrolizidine alkaloids (PAs). The results revealed that diester-type PAs had more pronounced effects than monoester-type PAs, including cytotoxicity, DNA damage, and cell cycle perturbation. Additionally, in vitro experiments showed that lasiocarpine had aneugenic effects.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Mirko Marino, Letizia Gigliotti, Peter Moller, Patrizia Riso, Marisa Porrini, Cristian Del Bo
Summary: The comet assay is used to assess DNA damage in tissues and biological samples. This study found that storage time had varying effects on DNA damage levels in PBMCs and WB samples, with significant increases observed in WB samples stored for over 6 months. WB samples showed higher levels of DNA damage compared to PBMCs.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Arun Kumar, Sandeep Choudhary, Somesh Kumar, Jawahar S. Adhikari, Seema Kapoor, Nabo K. Chaudhury
Summary: Melatonin plays a role in recovering the hematopoietic system of irradiated mice by inducing G-CSF mediated radioprotection and related mechanisms. Different doses of melatonin, with 20mg/kg as the optimal dose, effectively reduce radiation-induced DNA damage and cell death.
Review
Environmental Sciences
Aryelle Canedo, Thiago Lopes Rocha
Summary: Zebrafish have become a popular model for assessing the genotoxicity of pollutants due to their orthologous genes and involvement in repair pathways. Comet assay is the main technique used in these studies, but standardization is needed for better interpretation and comparisons. Understanding repair mechanisms in zebrafish subjected to DNA damage is essential for further research on genotoxic effects caused by pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Marcelino Benvindo-Souza, Daiany Folador Sotero, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Rinneu Elias Borges, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Pollution from mining activities can cause genotoxic damage to bats, particularly in species like Carollia perspicillata and Glossophaga soricina. High levels of elements such as Mn, Cr, Pb, and Zn were found in water samples from the mining area. The study suggests that bats in mining areas are susceptible to increased DNA damage, similar to what has been observed in other species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Vehbi Atahan Togay, Gulcin Yavuz Turel, Dilek Asci celik, Meltem ozgocmen, Eda Evgen Tuluceoglu, Ismail Sen, Yusuf Ayvaz
Summary: This study found that cyprodinil and thiacloprid exposure can lead to increased DNA damage risk in zebrafish, as demonstrated by comet assay. These pesticides were identified as genotoxic agents that require further investigation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Amelie Cant, Anne Bado-Nilles, Jean-Marc Porcher, Dorothee Bolzan, Jean Prygiel, Audrey Catteau, Cyril Turies, Alain Geffard, Marc Bonnard
Summary: Aquatic species are exposed to various substances, which can harm their genetic integrity through DNA damage or oxidative stress. DNA strand breaks and chromosomal damages have been proven to be relevant biomarkers in aquatic biomonitoring. However, these biomarkers do not reflect DNA oxidative lesions, such as 8-oxodG, which are considered pre-mutagenic lesions in human biomonitoring.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Rafael Valencia-Quintana, Mirta Milic, Stefano Bonassi, Maria Antonieta Ochoa-Ocana, Victoria Campos-Pena, Maria Guadalupe Tenorio-Arvide, Guillermo Alejandro Perez-Flores, Juana Sanchez-Alarcon
Summary: This study evaluated the effect of pesticides on genetic damage to agricultural workers in Los Reyes, Michoacan, Mexico. The results showed that pesticide use poses a risk of genetic damage to agricultural workers, but measures can be taken to reduce this risk.
Article
Pharmacology & Pharmacy
Jorid B. Sorlia, Alexander C. O. Jensen, Alicja Mortensen, Jozef Szarek, Claudia A. T. Gutierrez, Lucas Givelet, Katrin Loeschner, Charis Loizides, Iosif Hafez, George Biskos, Ulla Vogel, Niels Hadrup
Summary: By conducting nose-only inhalation tests on mice, we found that the air concentration of molybdenum disulphide particles has no observed adverse effect on body weight gain and genotoxicity below 13mg/m³, while the effects on respiratory function suggest this level as the lowest observed adverse effect concentration.
Article
Toxicology
Sherin John Joseph, Kantha Deivi Arunachalam, P. Balakrishna Murthy, Raghavendra Ramalingam, Mohamed Saiyad Musthafa
Summary: This study investigates the effects of uranium on human lymphocytes through acute toxicity research, revealing dose-dependent DNA damage and cell cycle arrest at the G2/M checkpoint, indicating decreased genetic stability. Lower doses of uranium exposure lead to better cell cycle responses and lower genetic damage index in human lymphocytes, suggesting adaptability and repair responses. The findings advance our understanding of uranium's impact on mammalian cells.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemistry & Molecular Biology
Tamara Bakuradze, Peter Meiser, Jens Galan, Elke Richling
Summary: This study found that consuming an aronia juice-based food supplement significantly reduced H2O2-induced DNA strand breaks, but had a low overall effect on background and total DNA strand breaks. This suggests that antioxidant supplements may only have marginal preventive effects under healthy conditions.
Article
Chemistry, Multidisciplinary
Sarah May, Cordula Hirsch, Alexandra Rippl, Alexander Buerkle, Peter Wick
Summary: This study investigated the genotoxicity of different engineered nanomaterials (ENMs) using two complementary in vitro assays. The results showed that certain ENMs exhibited cytotoxicity and genotoxicity, while others did not have these effects.