Luminescence-based in vivo monitoring of NF-κB activity through a gene delivery approach

Background: Monitoring activity of specific signaling pathways in vivo is challenging and requires highly sensitive methods to detect dynamic perturbations in whole organisms. Results: In vivo gene delivery of a luciferase reporter followed by bioluminiscence imaging allows measuring NF-kappa B activity in mice liver and lungs. Conclusions: This protocol allows a direct measure of NF-kappa B activity through quantification of bioluminescence signal, demonstrating its accuracy and sensitivity in different animal models and experimental conditions. Variants could be also applied for the analysis of NF-kappa B activity in different tissues or for studying other signaling pathways in vivo.