Article
Biochemistry & Molecular Biology
Rejina Shrestha, Naoko Murata-Kamiya, Satoshi Imai, Masami Yamamoto, Tetsuya Tsukamoto, Sachiyo Nomura, Masanori Hatakeyama
Summary: The initial step in bacterial infection is the adherence of the bacterium to the target cell surface. This study found that the mouse stomach has a much lower expression level of Ceacam1 compared to the human stomach, which leads to the inability of the bacteria to deliver the CagA protein due to the lack of interaction between HopQ and Ceacam1 in mouse gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Clara Lettl, Rainer Haas, Wolfgang Fischer
Summary: Type IV secretion of effector proteins is crucial for interaction of human pathogens with target cells, with the example of CagA effector protein produced by Helicobacter pylori. A novel split luciferase-based translocation reporter system was developed to study the kinetics and mode of action of CagA type IV secretion, showing rapid injection of CagA into gastric epithelial cells by H. pylori cells. It was found that unfolding of effector proteins is necessary for transport, providing new insights into the translocation mechanisms of pathogenic bacteria.
MOLECULAR MICROBIOLOGY
(2021)
Article
Food Science & Technology
Aina Ichihara, Hinako Ojima, Kazuyoshi Gotoh, Osamu Matsushita, Susumu Take, Hiroyuki Okada, Akari Watanabe, Kenji Yokota
Summary: This study aimed to explore the association between different antibody reactivities and bacterial genome organization. High-reactive strains showed high gene homology, while inversion around the vacA gene was found in the genome of poorly responsive antigen strains.
Article
Biochemistry & Molecular Biology
Jacky Lu, Kathryn P. Haley, Jamisha D. Francis, Miriam A. Guevara, Ryan S. Doster, Kelly M. Craft, Rebecca E. Moore, Schuyler A. Chambers, Alberto G. Delgado, Maria Blanca Piazuelo, Steven M. Damo, Steven D. Townsend, Jennifer A. Gaddy
Summary: Chronic infection with Helicobacter pylori increases the risk of gastric diseases, including gastric cancer, with strains encoding the cag T4SS system being associated with more serious disease outcomes. The presence of nutrient iron can modulate the activity of the T4SS and biogenesis of T4SS pili, with lactoferrin exerting antimicrobial activity against H. pylori under iron-limited conditions. Additionally, culturing H. pylori with holo-lactoferrin prior to co-culture with gastric epithelial cells can lead to repression of the cag T4SS activity and decreased biogenesis of T4SS pili.
Article
Immunology
Sirena C. Tran, Mark S. Mcclain, Timothy L. Cover
Summary: Helicobacter pylori strains containing the cag pathogenicity island (PAI) are associated with the development of gastric adenocarcinoma and peptic ulcer disease. The cag PAI encodes a secreted effector protein (CagA) and a type IV secretion system (Cag T4SS). This study investigated the role of the CagY antenna region in Cag T4SS OMCC assembly and function.
INFECTION AND IMMUNITY
(2023)
Review
Biochemistry & Molecular Biology
Silvia Salvatori, Irene Marafini, Federica Laudisi, Giovanni Monteleone, Carmine Stolfi
Summary: H. pylori infection contributes to the onset and development of gastric cancer through the mechanisms of chronic inflammation and DNA damage to gastric epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Yoshiaki Usui, Yukari Taniyama, Mikiko Endo, Yuriko N. Koyanagi, Yumiko Kasugai, Isao Oze, Hidemi Ito, Issei Imoto, Tsutomu Tanaka, Masahiro Tajika, Yasumasa Niwa, Yusuke Iwasaki, Tomomi Aoi, Nozomi Hakozaki, Sadaaki Takata, Kunihiko Suzuki, Chikashi Terao, Masanori Hatakeyama, Makoto Hirata, Kokichi Sugano, Teruhiko Yoshida, Yoichiro Kamatani, Hidewaki Nakagawa, Koichi Matsuda, Yoshinori Murakami, Amanda B. Spurdle, Keitaro Matsuo, Yukihide Momozawa
Summary: This study reveals that certain genetic variants associated with Helicobacter pylori infection can increase the risk of gastric cancer. Individuals carrying these genetic variants and infected with H. pylori have an even higher risk of developing gastric cancer.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Microbiology
Yi Ying Cheok, Chalystha Yie Qin Lee, Heng Choon Cheong, Jamuna Vadivelu, Chung Yeng Looi, Suhailah Abdullah, Won Fen Wong
Summary: Helicobacter pylori, armed with unique virulence factors, is capable of surviving in the harsh stomach environment and causing inflammation and tissue damage, leading to gastric cancer. The bacterium not only establishes colonization in the stomach, but also manipulates the host immune system to ensure long-term survival.
Article
Cardiac & Cardiovascular Systems
Xiujuan Xia, Linfang Zhang, Hao Wu, Feng Chen, Xuanyou Liu, Huifang Xu, Yuqi Cui, Qiang Zhu, Meifang Wang, Hong Hao, De-Pei Li, William P. Fay, Luis A. Martinez-Lemus, Michael A. Hill, Canxia Xu, Zhenguo Liu
Summary: This study found that CagA(+) H. pylori effectively colonizes gastric mucosa and impairs endothelial function, leading to increased atherosclerosis through exosomes-mediated ROS formation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Infectious Diseases
Tomomi Kobatake, Keiki Ogino, Hiroyuki Sakae, Kazuyoshi Gotoh, Akari Watanabe, Osamu Matsushita, Hiroyuki Okada, Kenji Yokota
Summary: The study found that disulfiram has bactericidal effects on Helicobacter pylori at low concentrations, suggesting it can serve as a supplement to current H. pylori eradication drugs.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Multidisciplinary Sciences
Bui Hoang Phuc, Vo Phuoc Tuan, Ho Dang Quy Dung, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ngo Phuong Minh Thuan, Vu Van Khien, Tran Thi Huyen Trang, Junko Akada, Takeshi Matsumoto, Yoshio Yamaoka
Summary: The acquisition of tfs3/4 ICE was common in H. pylori strains in patients with gastroduodenal disease in Vietnam, and the complete cluster of tfs3 ICE was a reliable marker for the severity of disease in the H. pylori infected population.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Bui Hoang Phuc, Vo Phuoc Tuan, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ho Dang Quy Dung, Ngo Phuong Minh Thuan, Kartika Afrida Fauzia, Evariste Tshibangu-Kabamba, Ricky Indra Alfaray, Batsaikhan Saruuljavkhlan, Takashi Matsumoto, Junko Akada, Yoshio Yamaoka
Summary: This study reports the complete genomes of two Helicobacter pylori strains, CHC155 and VN1291, isolated from a non-cardia gastric cancer patient and a duodenal ulcer patient, respectively, and demonstrates their virulence through in vitro infection. The genomes of both strains possess four kinds of genomic islands related to H. pylori pathogenicity. These genomes also carry genes associated with antibiotic resistance, which are risk factors in the development of gastric cancer and duodenal ulcer.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Zong-Bao Yan, Jin-Yu Zhang, Yi-Pin Lv, Wen-Qing Tian, Zhi-Guo Shan, Fang-Yuan Mao, Yu-Gang Liu, Wan-Yan Chen, Pan Wang, Yun Yang, Ping Cheng, Liu-Sheng Peng, Ya-Ling Liao, Geng-Yu Yue, Xiao-Lin Xu, Yong-Liang Zhao, Mu-Han Lu, Yuan Zhuang
Summary: The study found that REDD1 is increased in the gastric mucosa of H. pylori-infected patients and mice. H. pylori induced GECs to express REDD1 via the phosphorylated cagA, activating the MAPKp38 pathway. In Redd1-/- mice, gastric inflammation, MHCII+ monocyte infiltration, IL-23, and IL-17A were all attenuated.
Article
Food Science & Technology
Sebastian Diechler, Bianca E. Chichirau, Gernot Posselt, Dionyssios N. Sgouras, Silja Wessler
Summary: Research has shown that the diverse structure of CagA regulates B cell physiology, while B cell survival is independent of CagA.
Article
Gastroenterology & Hepatology
Sungil Jang, Lori M. Hansen, Hanfu Su, Jay Solnick, Jeong-Heon Cha
Summary: This study found that the number of cagA copies in Helicobacter pylori is higher in mice with less intense immune response and lower in mice with more intense immune response. Additionally, cagY recombination is also associated with H. pylori virulence.
Article
Cell Biology
Gernot Posselt, Maria Wiesauer, Bianca E. Chichirau, Daniela Engler, Linda M. Krisch, Gabriele Gadermaier, Peter Briza, Sabine Schneider, Francesco Boccellato, Thomas F. Meyer, Cornelia Hauser-Kronberger, Daniel Neureiter, Anne Mueller, Silja Wessler
CELL COMMUNICATION AND SIGNALING
(2019)
Review
Food Science & Technology
Bianca E. Chichirau, Sebastian Diechler, Gernot Posselt, Silja Wessler
Article
Biochemistry & Molecular Biology
Theresa Neuper, Tobias Frauenlob, Muamera Sarajlic, Gernot Posselt, Silja Wessler, Jutta Horejs-Hoeck
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Gastroenterology & Hepatology
Markus M. Heimesaat, Anna-Maria Schmidt, Soraya Mousavi, Ulrike Escher, Nicole Tegtmeyer, Silja Wessler, Gabriele Gadermaier, Peter Briza, Dirk Hofreuter, Stefan Bereswill, Steffen Backert
Article
Biochemistry & Molecular Biology
Alex T. Mueller, Gernot Posselt, Gisela Gabernet, Claudia Neuhaus, Simon Bachler, Markus Blatter, Bernhard Pfeiffer, Jan A. Hiss, Petra S. Dittrich, Karl-Heinz Altmann, Silja Wessler, Gisbert Schneider
Article
Cell Biology
Muamera Sarajlic, Theresa Neuper, Julia Vetter, Susanne Schaller, Maria M. Klicznik, Iris K. Gratz, Silja Wessler, Gernot Posselt, Jutta Horejs-Hoeck
CELL COMMUNICATION AND SIGNALING
(2020)
Article
Immunology
Bianca E. Chichirau, Tamara Scheidt, Sebastian Diechler, Theresa Neuper, Jutta Horejs-Hoeck, Christian G. Huber, Gernot Posselt, Silja Wessler
PATHOGENS AND DISEASE
(2020)
Review
Microbiology
Miroslaw Jarzab, Gernot Posselt, Nicole Meisner-Kober, Silja Wessler
Article
Food Science & Technology
Sebastian Diechler, Bianca E. Chichirau, Gernot Posselt, Dionyssios N. Sgouras, Silja Wessler
Summary: Research has shown that the diverse structure of CagA regulates B cell physiology, while B cell survival is independent of CagA.
Article
Cell Biology
Sabine Bernegger, Robert Vidmar, Marko Fonovic, Gernot Posselt, Boris Turk, Silja Wessler
Summary: Through proteomic analysis, novel extracellular substrates of HpHtrA, including hDsg2, were identified, along with the well characterized target hCdh1. The study demonstrated efficient hDsg2 cleavage by HpHtrA, emphasizing its importance in opening intercellular junctions.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Sabine Bernegger, Miroslaw Jarzab, Silja Wessler, Gernot Posselt
Summary: This article discusses the importance of proteases in gastric cancer, including their role in various stages of tumor development, such as epithelial inflammation, cell proliferation and cell death, tumor metastasis and neoangiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sabine Bernegger, Evelyn Hutterer, Urszula Zarzecka, Thomas P. Schmidt, Markus Huemer, Isabella Widlroither, Gernot Posselt, Joanna Skorko-Glonek, Silja Wessler
Summary: Helicobacter pylori expresses the serine protease and chaperone High temperature requirement A (HtrA), which cleaves the human cell adhesion molecule E-cadherin. This mechanism is prevalent in various pathogens and has been shown to be equally effective in mice and dogs.
Article
Pharmacology & Pharmacy
Alaa Alhayek, Essak S. Khan, Esther Schoenauer, Tobias Daeinghaus, Roya Shafiei, Katrin Voos, Mitchell K. L. Han, Christian Ducho, Gernot Posselt, Silja Wessler, Hans Brandstetter, Joerg Haupenthal, Aranzazu del Campo, Anna K. H. Hirsch
Summary: Collagenases produced by Bacillus cereus play an important role in wound infections. Targeting collagenases instead of bacteria could be a promising strategy for treating such infections. This study characterizes the collagenolytic activity of secreted collagenases and demonstrates their role in damaging dermal collagen, which facilitates the spread of bacteria. Additionally, the importance of collagenases in disease promotion is highlighted through the use of inhibitors.
ADVANCED THERAPEUTICS
(2022)
Article
Microbiology
Deepthi Radhakrishnan, M. C. Amrutha, Evelyn Hutterer, Silja Wessler, Karthe Ponnuraj
Summary: HtrA is a secreted virulence factor in many pathogenic bacteria, with varying interactions with ECM molecules and host proteins. The PDZ domain plays a crucial role in substrate cleavage, potentially contributing to the pathogenicity of HtrA in L. monocytogenes.
FEMS MICROBIOLOGY LETTERS
(2021)
Correction
Chemistry, Multidisciplinary
Dana Westmeier, Gernot Posselt, Angelina Hahlbrock, Sina Bartfeld, Cecilia Vallet, Carmen Abfalter, Dominic Docter, Shirley K. Knauer, Silja Wessler, Roland H. Stauber
