Review
Medicine, Research & Experimental
Jianyang Liu, Yan Huang, Ting Li, Zheng Jiang, Liuwang Zeng, Zhiping Hu
Summary: The Golgi apparatus plays a crucial role in cellular homeostasis and is linked to various diseases. Mutations in genes encoding Golgi resident proteins can lead to diseases, typically involving defects in membrane trafficking, protein mislocalization, and impaired glycosylation. Detection of Golgi resident proteins in human serum samples may serve as a diagnostic tool, while the central role of Golgi apparatus in membrane trafficking pathways presents potential targets for disease therapy.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Plant Sciences
Enrico Cortese, Roberto Moscatiello, Francesca Pettiti, Luca Carraretto, Barbara Baldan, Lorenzo Frigerio, Ute C. Vothknecht, Ildiko Szabo, Diego De Stefani, Marisa Brini, Lorella Navazio
Summary: This study successfully achieved precise measurements of dynamic changes in free Ca2+ concentration in the lumen of the plant endoplasmic reticulum using an aequorin chimera with reduced Ca2+ affinity, revealing rapid and transient increases of the ER luminal Ca2+ concentration in response to different environmental stresses. Comparative analysis also indicates a complex interplay between ER and chloroplast Ca2+ dynamics in shaping cytosolic Ca2+ signals during signal transduction events.
Article
Horticulture
Chayce Griffith, Todd C. Einhorn
Summary: Bitter pit, a physiological disorder, causes significant losses in apple fruit production, especially for the cultivar 'Honeycrisp'. Increasing calcium levels in fruit does not fully prevent bitter pit, indicating it is a complex disorder influenced by various factors including hormones and plant growth regulators. Auxins promote xylem function and calcium transport, gibberellins exacerbate bitter pit by reducing membrane stability, and abscisic acid mitigates it by improving xylem function and facilitating calcium transport.
SCIENTIA HORTICULTURAE
(2023)
Review
Cell Biology
Akihiko Nakano
Summary: The structure of the Golgi apparatus varies among different species, but its functions remain conserved. In addition to the core layers of cisternae, the Golgi is accompanied by neighboring compartments on its cis and trans sides. High-resolution live imaging has revealed striking similarities in the behaviors of these compartments, indicating the presence of conserved mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lana Buzuk, Doris Hellerschmied
Summary: The Golgi apparatus plays a crucial role in the secretory pathway of eukaryotic cells, processing proteins and organizing their transportation to other cellular compartments. It is involved in cell surface formation, cell polarity, cell-cell communication, immune signaling, DNA damage response, and mitosis. Maintaining Golgi integrity and protein homeostasis is essential, as Golgi fragmentation and dysfunction are linked to neurodegenerative diseases and certain cancers. Recent studies have revealed the importance of ubiquitin signaling in maintaining Golgi integrity and protein quality control, similar to the endoplasmic reticulum. Ubiquitination helps prevent the accumulation of toxic protein aggregates and regulates Golgi structural rearrangements in response to cellular stress.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Meilan Chen, Lu Xu, Yi Wu, Peter Soba, Chun Hu
Summary: Dendrites are specialized compartments in neurons that sense, integrate, and transfer information. Abnormal dendrite development is strongly linked to neurological disorders. The Golgi apparatus, located at the center of the secretory pathway, plays a crucial role in posttranslational modifications, sorting, transport, and signal transduction. It also forms distinct structures known as Golgi outposts in dendrites. However, the organization and function of Golgi in dendrite development and its impact on neurological disorders are still not well understood and lack a comprehensive summary.
Review
Plant Sciences
Yoko Ito, Tomohiro Uemura
Summary: Compared to the static image of plants, the world inside each cell is dynamic. Visualizing the working components inside cells using super-resolution microscopy is crucial for understanding cellular events, particularly in the study of plant membranes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ke Li, Ximeng Zhou, Xueping Sun, Guanghui Li, Lei Hou, Shuzhen Zhao, Chuanzhi Zhao, Changle Ma, Pengcheng Li, Xingjun Wang
Summary: Ribosomes are essential for plant growth and development, and their dysfunction can affect auxin response. In mutants with ribosome biogenesis deficiency like mdn1-1, genes involved in auxin biosynthesis, transport, and signaling are altered, and the protein distribution of PIN2 in roots is affected. Additionally, MDN1 is an auxin-responsive gene targeted by ARFs, suggesting a coordination mechanism between auxin and ribosome biogenesis for regulating plant development.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Liang Zhang, Jingwen Ma, Huan Liu, Qian Yi, Yanan Wang, Jingjing Xing, Peipei Zhang, Shengdong Ji, Mingjun Li, Jingyuan Li, Jinbo Shen, Jinxing Lin
Summary: The R-SNAREs VAMP721 and VAMP722 in Arabidopsis are found to play crucial roles in intracellular trafficking, affecting auxin distribution and plant growth by regulating the polar localization of auxin transporters.
Editorial Material
Plant Sciences
Daiyan Li, Zhongtao Jia
Summary: The study shows that auxin dynamics play a critical role in facilitating plant root penetration into hard soil barriers. By mapping the genetic locus associated with primary root penetrability, new insights into the regulatory role of auxin signaling in root penetrability and related natural genetic variation for this important trait are gained.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Qingchen Rui, Xiaoyun Tan, Feng Liu, Yiqun Bao
Summary: In this review, we summarize the key players in Golgi maintenance demonstrated by genetic studies in plants, with an emphasis on their roles in intra-Golgi trafficking.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Anatomy & Morphology
Daniel Talamas-Lara, Karla Acosta-Virgen, Bibiana Chavez-Munguia, Anel Lagunes-Guillen, Lizbeth Salazar-Villatoro, Martha Espinosa-Cantellano, Adolfo Martinez-Palomo
Summary: The study discovered previously undetected Golgi apparatus components in the cytoplasm of E. histolytica and E. dispar, challenging the traditional view that these amoebae lack Golgi apparatus.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Chemistry, Multidisciplinary
Weiyi Tan, Qiuxin Zhang, Jiaqing Wang, Meihui Yi, Hongjian He, Bing Xu
Summary: Introducing a sulfur atom into phosphopeptides enables rapid targeting of Golgi apparatus and selective killing of cancer cells. The resulting thiophosphopeptide self-assembles after alkaline phosphatase-catalyzed dephosphorylation, enters cells, and accumulates in Golgi apparatus, inhibiting cancer cells effectively.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Rong Sheng Li, Jiahui Liu, Hu Shi, Ping Ping Hu, Yao Wang, Peng Fei Gao, Jian Wang, Moye Jia, Hongwei Li, Yuan Fang Li, Chengde Mao, Na Li, Cheng Zhi Huang
Summary: Golgi apparatus-targeted nano-mechanical disruption is an attractive approach for killing cancer cells by multimodal mechanism and avoiding drug resistance. The transformable peptide C6RVRRF4KY can self-assemble into nontoxic nanoparticles in aqueous medium but transformed into left-handed helical fibrils (L-HFs) after targeting and furin cleavage in the Golgi apparatus of cancer cells, leading to mechanical disruption and death of cancer cells without acquired drug resistance. This nanomechanical disruption concept should also be applicable to multidrug-resistant bacteria and viruses.
Article
Biochemistry & Molecular Biology
Vladimir Skalicky, Tereza Vojtkova, Ales Pencik, Jan Vrana, Katarzyna Juzon, Veronika Kolackova, Michaela Sedlarova, Martin F. Kubes, Ondrej Novak
Summary: This study tested various nuclear isolation protocols and combined with mass spectrometry analysis to obtain information on the auxinome from isolated nuclei of Arabidopsis and tobacco cell cultures, revealing dynamic changes in auxin homeostasis within the nucleus. The methodological procedure combining flow cytometry and mass spectrometry offers new horizons for the study of auxin homeostasis at the subcellular level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Ricardo Nilo-Poyanco, Paula Vizoso, Dayan Sanhueza, Ivan Balic, Claudio Meneses, Ariel Orellana, Reinaldo Campos-Vargas
PHYSIOLOGIA PLANTARUM
(2019)
Article
Agriculture, Multidisciplinary
Talia del Pozo, Simon Miranda, Mauricio Latorre, Felipe Olivares, Leonardo Pavez, Ricardo Gutierrez, Jonathan Maldonado, Patricio Hinrichsen, Bruno G. Defilippi, Ariel Orellana, Mauricio Gonzalez
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Elena A. Vidal, Tomas C. Moyano, Bernabe Bustos, Eduardo Perez-Palma, Carol Moraga, Eleodoro Riveras, Alejandro Montecinos, Lorena Azocar, Daniela C. Soto, Mabel Vidal, Alex Di Genoval, Klaus Puschel, Peter Nurnberg, Stephan Buch, Jochen Hampe, Miguel L. Allende, Veronica Cambiazo, Mauricio Gonzalez, Christian Hodar, Martin Montecino, Claudia Munoz-Espinoza, Ariel Orellana, Angelica Reyes-Jara, Dante Travisanyl, Paula Vizoso, Mauricio Moraga, Susana Eyheramendy, Alejandro Maass, Giancarlo De Ferrari, Juan Francisco Mique, Rodrigo A. Gutierrez
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Juan Pablo Parra-Rojas, Asier Largo-Gosens, Tomas Carrasco, Jonathan Celiz-Balboa, Veronica Arenas-Morales, Pablo Sepulveda-Orellana, Henry Temple, Dayan Sanhueza, Francisca C. Reyes, Claudio Meneses, Susana Saez-Aguayo, Ariel Orellana
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Plant Sciences
Carlos Rubilar-Hernandez, Claudio Osorio-Navarro, Francisca Cabello, Lorena Norambuena
Article
Plant Sciences
Claudia Munoz-Espinoza, Alex Di Genova, Alicia Sanchez, Jose Correa, Alonso Espinoza, Claudio Meneses, Alejandro Maass, Ariel Orellana, Patricio Hinrichsen
Article
Biotechnology & Applied Microbiology
Ricardo Nilo-Poyanco, Carol Moraga, Gianfranco Benedetto, Ariel Orellana, Andrea Miyasaka Almeida
Summary: Shotgun proteomics is an unbiased approach to study the proteome of mature and ripe fruit in Prunus persica, revealing critical molecular processes involved in fruit ripening. Analysis showed that about 15% of proteins identified were modulated by ripening, with processes such as glycogen and isocitrate metabolism, protein localization, and cell wall modification being overrepresented. Additionally, potential biomarkers for ripening included proteins involved in pectin metabolism, hormone synthesis and transcription factor regulation.
Article
Plant Sciences
Jonathan Celiz-Balboa, Asier Largo-Gosens, Juan Pablo Parra-Rojas, Veronica Arenas-Morales, Pablo Sepulveda-Orellana, Hernan Salinas-Grenet, Susana Saez-Aguayo, Ariel Orellana
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Multidisciplinary Sciences
Claudio Olmos, Gonzalo Campana, Victor Monreal, Paola Pidal, Nannet Sanchez, Constanza Airola, Dayan Sanhueza, Patricio Tapia, Ana Maria Munoz, Felipe Corvalan, Sebastian Hurtado, Claudio Meneses, Ariel Orellana, Martin Montecino, Gloria Arriagada, Fernando Jose Bustos
Summary: Asymptomatic SARS-CoV-2 infection was detected in 3.14% of healthcare workers in a private clinic in Santiago, Chile. Most of the infected individuals did not show symptoms within 3 weeks of testing, and genetic analysis suggested community infection as the main source of transmission among healthcare workers. The study indicated that the protocols implemented in the clinic were effective in preventing nosocomial infection clusters caused by asymptomatic carriers.
Article
Biotechnology & Applied Microbiology
Alex Di Genova, Gino Nardocci, Rodrigo Maldonado-Agurto, Christian Hodar, Camilo Valdivieso, Pamela Morales, Felipe Gajardo, Raquel Marina, Rodrigo A. Gutierrez, Ariel Orellana, Veronica Cambiazo, Mauricio Gonzalez, Alvaro Glavic, Marco A. Mendez, Alejandro Maass, Miguel L. Allende, Martin A. Montecino
Summary: The study on Orestias ascotanensis from the Chilean Altiplano reveals a genomic evolution process involving gene contraction and expansion, particularly in genes related to genome stability and DNA repair under positive selection to cope with high-altitude aquatic environments.
Article
Immunology
Paola Pidal, Jorge Fernandez, Constanza Airola, Miguel Araujo, Ana Maria Menjiba, Hector San Martin, Nicole Bruneau, Monserrat Balanda, Coral Elgueta, Rodrigo Fasce, Maria Teresa Valenzuela, Ariel Orellana, Eugenio Ramirez
Summary: Understanding the host immune response to SARS-CoV-2 variants of concern is crucial for improving diagnostics, therapy development, and vaccines. This study analyzed the level of neutralizing antibodies against different variants in healthcare workers previously infected with the D614G variant. The results showed that neutralizing antibody levels varied among different variants and changed over time during the follow-up period. There was evidence of immune escape from neutralizing antibodies with the Omicron variant due to mutations in the S protein.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Plant Sciences
Henry Temple, Pyae Phyo, Weibing Yang, Jan J. Lyczakowski, Alberto Echevarria-Poza, Igor Yakunin, Juan Pablo Parra-Rojas, Oliver M. Terrett, Susana Saez-Aguayo, Ray Dupree, Ariel Orellana, Mei Hong, Paul Dupree
Summary: Polysaccharide methylation, particularly of pectin, is a crucial feature of land plant cell walls. This study identifies putative Golgi SAM transporters in Arabidopsis and shows their significance in the import of SAM molecules for polysaccharide methylation in the Golgi, ultimately affecting cell wall structure and function.
Article
Multidisciplinary Sciences
Gil Eshel, Viviana Araus, Soledad Undurraga, Daniela C. Soto, Carol Moraga, Alejandro Montecinos, Tomas Moyano, Jonathan Maldonado, Francisca P. Diaz, Kranthi Varala, Chase W. Nelson, Orlando Contreras-Lopez, Henrietta Pal-Gabor, Tatiana Kraiser, Gabriela Carrasco-Puga, Ricardo Nilo-Poyanco, Charles M. Zegar, Ariel Orellana, Martin Montecino, Alejandro Maass, Miguel L. Allende, Robert DeSalle, Dennis W. Stevenson, Mauricio Gonzalez, Claudio Latorre, Gloria M. Coruzzi, Rodrigo A. Gutierrez
Summary: The Atacama Desert in Chile is a harsh environment with extreme conditions, yet it supports a diverse range of plant species including endemic plants. Research conducted in the region has identified genes associated with plant adaptation to stressful environments, including those linked to stress responses, metabolism, and energy production. This genetic information could be valuable for engineering crop resilience to climate change, especially as some Atacama plants are closely related to staple crops.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Susana Saez-Aguayo, Juan Pablo Parra-Rojas, Pablo Sepulveda-Orellana, Jonathan Celiz-Balboa, Veronica Arenas-Morales, Christine Salle, Hernan Salinas-Grenet, Asier Largo-Gosens, Helen M. North, Marie-Christine Ralet, Ariel Orellana
Summary: The biosynthesis of Rhamnogalacturonan-I involves transport of UDP-Rhamnose into the Golgi apparatus by URGT transporters. URGT2, URGT4, and URGT6 play distinct roles in mucilage biosynthesis, with URGT2 and URGT4 mainly involved in RG-I elongation and URGT6 affecting adherent mucilage. Mutants lacking all three transporters show changes in polymer chains and xylan production.
Meeting Abstract
Chemistry, Multidisciplinary
Marie-Christine Ralet, Susana Saez-Aguayo, Ariel Orellana, Helen North
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.
