期刊
CELL CALCIUM
卷 52, 期 3-4, 页码 217-225出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2012.06.009
关键词
Secretory vesicles; Calcium dynamics; Confocal microscopy; Fluctuation analysis; Super-Poisson statistics
类别
资金
- NIH
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- US-Israel Binational Science Foundation
- Israeli Science Foundation
Calcium (Ca2+) dynamics were evaluated in fluorescently labeled sea urchin secretory vesicles using confocal microscopy. 71% of the vesicles examined exhibited one or more transient increases in the fluorescence signal that was damped in time. The detection of transient increases in signal was dependent upon the affinity of the fluorescence indicator; the free Ca2+ concentration in the secretory vesicles was estimated to be in the range of similar to 10 to 100 mu M. Non-linear stochastic analysis revealed the presence of extra variance in the Ca2+ dependent fluorescence signal. This noise process increased linearly with the amplitude of the Ca2+ signal. Both the magnitude and spatial properties of this noise process were dependent upon the activity of vesicle p-type (Ca(nu)2.1) Ca2+ channels. Blocking the p-type Ca2+ channels with omega-agatoxin decreased signal variance, and altered the spatial noise pattern within the vesicle. These fluorescence signal properties are consistent with vesicle Ca2+ dynamics and not simply due to obvious physical properties such as gross movement artifacts or pH driven changes in Ca2+ indicator fluorescence. The results suggest that the free Ca2+ content of cortical secretory vesicles is dynamic; this property may modulate the exocytotic fusion process. Published by Elsevier Ltd.
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