Article
Physiology
Declan Manning, Richard Barrett-Jolley, Richard L. Evans, Caroline Dart
Summary: Skin is the largest organ in the human body, mainly composed of keratinocytes which maintain a healthy skin barrier through regulated differentiation driven by Ca2+-transcriptional coupling. Disruption of this process leads to various skin conditions. Our study suggests that TRPC2 and Orai1 channels do not insert into the plasma membrane during store-operated Ca2+ entry.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Neurosciences
Pragnya Chakraborty, Gaiti Hasan
Summary: Key components of endoplasmic reticulum (ER) Ca2+ release and store-operated Ca2+ entry (SOCE) are expressed in all metazoan cells. This review presents evidence on how these two mechanisms impact neuronal physiology and their interaction with ion channels in neurons. The careful regulation of neural SOCE is essential due to the detrimental effects of Ca2+ imbalance on neurons. The study also discusses different mechanisms of SOCE regulation in Drosophila and mammalian neurons, and their significance in human neurodegenerative diseases.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Review
Cell Biology
Ting Lu, Yihua Zhang, Yong Su, Dayan Zhou, Qiang Xu
Summary: Store-operated channels (SOCs) are highly selective Ca2+ channels that mediate Ca2+ influx in various cells. These channels are activated by Ca2+ depletion of the endoplasmic reticulum and sarcoplasmic reticulum, independently of inositol 1,4,5-trisphosphate (InsP3). SOC plays critical roles in endothelial dysfunction and vascular smooth muscle proliferation, contributing to the progression of cardiovascular disease.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Vladislav Chubinskiy-Nadezhdin, Svetlana Semenova, Valeria Vasileva, Alla Shatrova, Natalia Pugovkina, Yuri Negulyaev
Summary: Endometrial mesenchymal stem cells (eMSCs) have the ability to differentiate into different cell types and are influenced by mechanical forces in their microenvironment. This study identified Piezo1 proteins as mechanosensitive channels in the plasma membrane of eMSCs, which transduce mechanical stimuli into intracellular signaling pathways. The influx of Ca2+ triggered by Piezo1 activity is regulated by store-operated Ca2+ entry (SOCE) through the ORAI1 channel and STIM1/STIM2 Ca2+ sensors. Activation of Piezo1 or SOCE does not cause cytotoxicity in eMSCs but affects their migratory capacity and cell proliferation. This finding suggests that Piezo1 and SOCE play important roles in the regulation of intracellular calcium, which influences the migratory activity and regenerative potential of eMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Inbal Dagan, Raz Palty
Summary: Calcium signaling in cells plays a crucial role in regulating cell survival, proliferation, and death. Store-operated Ca2+ entry through CRAC channels is a major pathway for Ca2+ influx in non-excitable cells, regulated by a negative feedback mechanism called slow Ca2+ dependent inactivation (SCDI).
Review
Physiology
Declan Manning, Caroline Dart, Richard L. Evans
Summary: The skin is a complex organ with various functions such as protection, temperature regulation, sensation, immune surveillance, and biochemical functions. Dysfunction in store-operated channels is linked to skin pathologies, and these channels play vital roles in the normal physiology and function of the skin.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yihan Shen, Nagendra Babu Thillaiappan, Colin W. Taylor
Summary: Increases in cytosolic Ca2+ concentration regulate cellular activities mainly through STIM1 and Orai1 mediated Ca2+ release and channel opening. Each native SOCE complex likely consists of only a few STIM1 dimers associated with a single Orai1 channel.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Jose Sanchez-Collado, Joel Nieto-Felipe, Isaac Jardin, Rajesh Bhardwaj, Alejandro Berna-Erro, Gines M. Salido, Tarik Smani, Matthias A. Hediger, Jose J. Lopez, Juan A. Rosado
Summary: Breast cancer cells exhibit altered expression and post-translational modification of STIM proteins and Orai channels, resulting in differences in store-operated Ca2+ entry (SOCE) compared to non-tumoral breast epithelial cells. This study showed that N-linked glycosylation of Orai1 and STIM1 is not essential for SOCE in breast cancer cells and may contribute to apoptosis resistance. Additionally, impairment of STIM1 N-linked glycosylation attenuated caspase-3 activation in non-tumoral cells, while breast cancer cells displayed smaller caspase-3 activity and were unaffected by the non-glycosylable STIM1 mutant. Abnormal SOCE and alterations in glycosylation are associated with cancer development and invasion.
Review
Cell Biology
Ewelina Latoszek, Magdalena Czeredys
Summary: The review highlights the essential role of SOCE in the physiology of neural stem cells and its dysregulation in HD pathology. Recent studies demonstrate the impact of elevated STIM2 expression on excessive Ca2+ entry in juvenile-onset HD-induced GABAergic cells. Progress in the in vitro differentiation of MSNs derived from various cell sources is discussed, along with the establishment of HD brain organoids for studying HD pathology and potential treatments. Stem cell therapy, particularly the transplantation of MSNs or their precursors, shows promise in restoring normal neural cell function in HD patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Razan Orfali, Ali AlFaiz, Mohammad Asikur Rahman, Liz Lau, Young-Woo Nam, Miao Zhang
Summary: K+ channels play crucial roles in lung physiology. The Ca2+-activated K+ channels (K-Ca) have recently gained more attention and efforts are being made to develop selective medications targeting these channels. Among the K-Ca channel family, the K(Ca)2 channel subtypes and the K(Ca)3.1 channel are voltage-independent K+ channels that mediate membrane hyperpolarization induced by Ca2+. This article discusses the different subtypes of K(Ca)2 and K(Ca)3.1 channels and their functions in respiratory diseases, as well as the pharmacology of these channels and their link to respiratory ciliary regulations.
Article
Biochemistry & Molecular Biology
Barbara Girolami, Matteo Serano, Antonio Michelucci, Laura Pietrangelo, Feliciano Protasi
Summary: This study demonstrates that exercise-induced assembly of CEUs may increase the risk of exertional heat stroke when physical activity is performed in adverse environmental conditions. Pre-exercised mice in both the trained-1 month and exercised-1 h groups showed higher internal temperatures after exertional stress, along with a greater number of CEUs in their muscles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elena Hernando-Perez, Enrique Perez-Riesgo, Santiago Cepeda, Ignacio Arrese, Rosario Sarabia, Carlos Villalobos, Lucia Nunez
Summary: Brain tumors consist of a variety of tumor types with different levels of malignancy. The study found that malignant tumor cells exhibit calcium responses to ATP and have significantly increased store-operated Ca2+ entry. Furthermore, high grade gliomas have larger SOCE compared to low grade gliomas, suggesting a correlation between SOCE and glioma progression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Oncology
Jose Sanchez-Collado, Jose J. Lopez, Carlos Cantonero, Isaac Jardin, Sergio Regodon, Pedro C. Redondo, Juan Gordillo, Tarik Smani, Gines M. Salido, Juan A. Rosado
Summary: The expression ratio of Orai1:Orai2 varies in different breast cancer cells, and Orai2 plays a crucial role in cell cycle progression and apoptosis resistance.
Article
Genetics & Heredity
Xuexue Zhu, Ke Ma, Kuo Zhou, Jibin Liu, Bernd Nuernberg, Florian Lang
Summary: The study showed that vasopressin enhances the expression of ORAI1 and STIM1, promoting Ca2+entry and osteogenic signaling. The effects of vasopressin-induced Ca2+ entry and osteogenic signaling can be disrupted by MRS1845 and GSK-650394.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Biochemistry & Molecular Biology
Yi-Shyun Lai, Ta-Wei Chan, Thi My Hang Nguyen, Tzu-Chien Lin, Yu-Ying Chao, Chia-Yih Wang, Liang-Yi Hung, Shaw-Jenq Tsai, Wen-Tai Chiu
Summary: This study investigates the regulation of cytosolic Ca2+ levels on ciliogenesis and finds that Ca2+ influx inhibits ciliogenesis while Ca2+ depletion promotes ciliogenesis. Additionally, store-operated Ca2+ entry and the endoplasmic reticulum Ca2+ sensor STIM1 negatively regulate ciliogenesis. The effects of different Ca2+ oscillation patterns on ciliogenesis are also explored using an optogenetic platform.
Article
Allergy
Jin-jin Zhang, Yi-yuan Cao, Ge Tan, Xiang Dong, Bin-chen Wang, Jun Lin, You-qin Yan, Guang-hui Liu, Mubeccel Akdis, Cezmi A. Akdis, Ya-dong Gao
Summary: The study aims to address the risk factors associated with the severity of COVID-19 patients and the mortality of severe patients. Survived severe and nonsurvived COVID-19 patients had distinct clinical and laboratory characteristics, which were separated by principal component analysis. Risk factors for mortality of severe COVID-19 patients include elder age, increased number of affected lobes, higher levels of serum CRP, chest tightness/dyspnea, and smoking history.
Article
Allergy
Hui Du, Xiang Dong, Jin-jin Zhang, Yi-yuan Cao, Mubeccel Akdis, Pei-qi Huang, Hong-wei Chen, Ying Li, Guang-hui Liu, Cezmi A. Akdis, Xiao-xia Lu, Ya-dong Gao
Summary: The COVID-19 pandemic caused by SARS-CoV-2 has had a significant impact worldwide. This study aimed to investigate the clinical characteristics of COVID-19 children with different severities and allergic status. The results showed that pediatric COVID-19 patients tend to have a mild clinical course, with pneumonia patients showing higher levels of fever, cough, and inflammatory biomarkers. Allergic and nonallergic COVID-19 children did not differ significantly in terms of disease incidence, clinical features, and laboratory and immunological findings.
Article
Medicine, General & Internal
Jian Shang, Qian Wang, Haiping Zhang, Xiaoyue Wang, Jing Wan, Youqin Yan, Yadong Gao, Jie Cheng, Ziang Li, Jun Lin
Summary: The study revealed that diabetic patients with COVID-19 showed more severe abnormalities in blood parameters compared to non-diabetic patients. Additionally, diabetic patients had higher rates of complications and adverse clinical outcomes such as respiratory failure, acute cardiac injury, and death. Therefore, diabetes is an independent risk factor for the prognosis of COVID-19, requiring special attention in prevention and treatment for this population.
AMERICAN JOURNAL OF MEDICINE
(2021)
Review
Allergy
Ya-dong Gao, Mei Ding, Xiang Dong, Jin-jin Zhang, Ahmet Kursat Azkur, Dilek Azkur, Hui Gan, Yuan-li Sun, Wei Fu, Wei Li, Hui-ling Liang, Yi-yuan Cao, Qi Yan, Can Cao, Hong-yu Gao, Marie-Charlotte Bruggen, Willem van de Veen, Milena Sokolowska, Mubeccel Akdis, Cezmi A. Akdis
Summary: The severity factors of COVID-19 include age, gender, underlying health conditions such as hypertension, diabetes, obesity, and immunodeficiency, which may lead to complications like acute kidney injury. Various laboratory indicators and parameters can help monitor disease progression, while socioeconomic factors, lifestyle, and quality of healthcare also play a role in individual outcomes.
Article
Allergy
Wei Fu, Shanshan Xu, Xiang Dong, Qing Xie, Yadong Gao
Summary: Aspirin-induced thrombocytopenia related to allergy is rarely reported. Symptoms of aspirin allergy included pruritus, purpura, thrombocytopenia, increased eosinophils, and lymph node enlargement. Discontinuation of aspirin led to resolution of symptoms in this case.
JOURNAL OF ASTHMA AND ALLERGY
(2021)
Article
Allergy
Debbie J. Maurer, Chengyao Liu, Paraskevi Xepapadaki, Barbara Stanic, Claus Bachert, Susetta Finotto, Ya-Dong Gao, Anna Graser, Tuomas Jartti, Walter Kistler, Marek Kowalski, Heikki Lukkarinen, Maria Pasioti, Ge Tan, Michael Villiger, Luo Zhang, Nan Zhang, Mubeccel Akdis, Nikolaos G. Papadopoulos, Cezmi A. Akdis
Summary: Physical activity levels are lower in asthmatic children, while high TV viewing time is associated with lower physical activity levels. Increasing physical activity can enhance cytokine release in response to various stimuli, potentially improving the immune response to infections.
Article
Allergy
Ying Zhou, Qingqing Li, Ruilin Pan, Qiong Wang, Xuming Zhu, Cunyin Yuan, Fangfang Cai, Ya-dong Gao, Yubao Cui
Summary: The study filled a gap by investigating the regulation of allergen gene expression by miRNA in Tyrophagus putresecentiae. It identified a regulatory network between miRNAs and allergen-encoding mRNAs throughout the mite's life cycle.
Review
Allergy
Silvana Fiorito, Marzia Soligo, Yadong Gao, Ismail Ogulur, Cezmi A. Akdis, Sergio Bonini
Summary: Research suggests that the high incidence and increased mortality of COVID-19 in Northern Italy may be attributed to severe air pollution and disrupted epithelial barriers. The region's high levels of fine particulate matter contribute to increased susceptibility to the virus, while barrier dysfunction caused by air pollution can lead to chronic inflammation and impaired immune response. These factors also contribute to the higher prevalence of comorbidities and therefore increased mortality from COVID-19.
Article
Allergy
Jin-jin Zhang, Xiang Dong, Guang-hui Liu, Ya-dong Gao
Summary: The outbreak of COVID-19 has become a global health crisis, with various factors such as age, sex, comorbidities, and racial/ethnic disparities influencing its morbidity. Children tend to experience milder illnesses, and factors like a healthy diet, sufficient nutrition, vaccination, and atopic conditions may act as protective factors. The effects of current therapies for COVID-19 and the bidirectional relationship between asthma and COVID-19 are still unclear.
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2023)
Review
Medicine, General & Internal
Xiang Dong, Mei Ding, Jinjin Zhang, Ismail Ogueluer, Yagiz Pat, Mubeccel Akdis, Yadong Gao, Cezmi A. Akdis
Summary: Type 2 inflammation and airway epithelial barrier interact closely in asthma. Dysfunction of the epithelial barrier may contribute to the development of asthma, highlighting the potential of restoring the airway epithelial barrier as a therapeutic strategy.
CHINESE MEDICAL JOURNAL
(2022)
Article
Allergy
Ya-Dong Gao, Paraskevi Xepapadaki, Yan-Wen Cui, Barbara Stanic, Debbie J. Maurer, Claus Bachert, Nan Zhang, Susetta Finotto, Maciej Chalubinski, Heikki Lukkarinen, Maria Passioti, Anna Graser, Tuomas Jartti, Marek Kowalski, Ismail Ogulur, Zi-Wei Shi, Mubeccel Akdis, Nikolaos G. Papadopoulos, Cezmi A. Akdis
Summary: This study investigated the impact of vaccinations against H. influenzae, pneumococcus, and influenza on respiratory infections, asthma symptoms, and immune responses in preschool children with asthma. The results showed that vaccination against H. influenzae and pneumococcus was associated with shorter duration of respiratory infections and reduced use of antibiotics. Influenza vaccination was associated with decreased occurrence of lower respiratory infections and gastrointestinal infections. The vaccinations also modulated the immune responses in asthmatic children.
Letter
Allergy
Silvana Fiorito, Marzia Soligo, Yadong Gao, Ismail Ogulur, Cezmi A. Akdis, Sergio Bonini
Letter
Allergy
Yin Wang, Huan Chen, Xiang Dong, Hao Chen, Hui-ling Liang, Ya-qi Yang, Yan-dan Chen, Rong-fei Zhu, Ya-dong Gao
CLINICAL AND TRANSLATIONAL ALLERGY
(2023)
Article
Allergy
Huan Chen, Guo-qing Gong, Mei Ding, Xiang Dong, Yuan-li Sun, Lang Wan, Ya-dong Gao
Summary: This study investigates the factors impacting dropout rates from sublingual immunotherapy (SLIT) in house dust mite (HDM)-sensitized allergic rhinitis patients. The study finds that the dropout rates increased over time, with most patients discontinuing SLIT after 1 year. Refusal of continuation and dissatisfaction with the efficacy were the main reasons for dropout.
FRONTIERS IN ALLERGY
(2022)
Review
Immunology
Ya-dong Gao, Ioana Agache, Mubeccel Akdis, Kari Nadeau, Ludger Klimek, Marek Jutel, Cezmi A. Akdis
Summary: Studies suggest that asthma and allergic diseases do not significantly affect susceptibility to or severity of COVID-19, but type 2 inflammation-related allergic diseases may impact susceptibility and progression. Current research is also exploring the impact and prevalence of allergic asthma and other allergic diseases in COVID-19 patients.
INTERNATIONAL IMMUNOLOGY
(2022)
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.