Involvement of interleukin-1β mediated nuclear factor κB signalling pathways to down-regulate prostate-specific antigen and cell proliferation in LNCaP prostate cancer cells

Involvement of NF-kappa B (nuclear factor kappa B) mediated by IL-1 beta (interleukin-1 beta) on cell proliferation and PSA (prostate-specific antigen) production of LNCaP prostate cell lines and the possible cross-talk with Akt (also known as protein kinase B) signalling pathway has been investigated. NF-kappa B and Akt were analysed by Western blotting from LNCaP cells treated by IL-1 beta before proliferation and PSA production were measured. IL-1 beta inhibited proliferation and decreased PSA production. The Akt pathway was not sensitive, whereas NF-kappa B phosphorylation occurred as a result of treatment. PSA production and proliferation of LNCaP cells were down-regulated by NF-kappa B mediated by IL-1 beta promoting anti-apoptotic signalling and co-suppressor factors of PSA expression. IL-1 beta through NF-kappa B activation provides a rationale for therapeutic approaches in the anticancer treatment of prostate.