期刊
CELL BIOLOGY INTERNATIONAL
卷 35, 期 2, 页码 165-169出版社
PORTLAND PRESS LTD
DOI: 10.1042/CBI20100346
关键词
breast cancer; CD3zeta; antibody microarray; ELISA; immune suppression
类别
资金
- Natural Science Foundation of Shanghai Jiao Tong University, School of Medicine [2008XJ023]
This work was done to establish a correlation between serum protein expression profiles and breast cancer. A high-density antibody microarray was used to identify serum protein expression profiles. Proteins up- or down-regulated by 2-fold compared with normal controls were used for hierarchical clustering analysis. ELISA and immunohistochemistry were used for validation of protein array data. The CD3zeta chain was one of the down-regulated molecules identified by the antibody array analysis and confirmed by ELISA and immunohistochemistry. The mean CD3zeta levels in breast cancer and in normal control were 119 +/- 23 and 156 +/- 35 ng/ml, respectively. The AUC (area under the curve) of CD3zeta was 0.837 +/- 0.057. Forty-one out of 65 breast cancer cases (63.08%) showed reduced CD3zeta expression on tumour-infiltrating lymphocytes by immunohistochemistry. This is the first report describing down-regulation of CD3zeta expression on breast cancer for the Chinese population and suggests that an immunosuppressive status plays an important role in breast cancer progression.
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