期刊
CELL BIOLOGY INTERNATIONAL
卷 35, 期 5, 页码 475-481出版社
WILEY
DOI: 10.1042/CBI20100372
关键词
cell density; mesenchymal stem cell; migration; nuclear factor-kappa B pathway; vascular cell adhesion molecule-1
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [22791935, 22791936, 18592026, 19791370]
- Keiryokai Research Foundation [100, 106]
- Grants-in-Aid for Scientific Research [22791936, 22791935, 19791370, 18592026, 23592896] Funding Source: KAKEN
MSCs (mesenchymal stem cells) migrate into damaged tissue and then proliferate and differentiate into various cell lineages to regenerate bone, cartilage, fat and muscle. Cell-cell adhesion of MSCs is essential for the MSC-dependent tissue regeneration after their homing into a damaged tissue. However, it remains to be elucidated what kinds of adhesion molecules play important roles in the cell-cell communication between MSCs. In order to identify adhesion molecules that facilitate mutual contact between MSCs, a comprehensive analysis of mRNA expression in adhesion molecules was performed by comparing profiles of expression status of adhesion molecules in MSCs at low- and high-cell density. We found that the expression level of VCAM1 (vascular cell adhesion molecule-1)/CD106 was clearly up-regulated in the human bone marrow-derived MSCs-UE7T-13 cells - under a condition of high cell density. Intriguingly, the migratory ability of the cells was clearly accelerated by a knockdown of VCAM1. Furthermore, the migratory ability of UE7T-13 cells was decreased by the over expression of exogenous VCAM1. In addition, the high cell density-induced expression of VCAM1 was clearly suppressed by NF-kappa B (nuclear factor-kappa B) signalling-related protein kinase inhibitors such as an IKK-2 (I kappa B kinase-2) inhibitor VI. In conclusion, the high cell density-induced VCAM1 expression through the NF-kappa B pathway inhibits the migratory ability of human bone marrow-derived MSCs.
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