Article
Dentistry, Oral Surgery & Medicine
Jie Liu, Xiaoling Wei, Junlong Hu, Xiaohan Tan, Xiaocui Kang, Li Gao, Ning Li, Xin Shi, Mengtong Yuan, Weiping Hu, Mingyue Liu
Summary: In this study, it was found that high concentrations of C5a can enhance odontoblast differentiation and increase DSP levels in DPSCs, while low concentrations of C5a have inhibitory effects. All six groups formed mineralized nodules after 28 days of culture.
Article
Chemistry, Multidisciplinary
Dua Abuarqoub, Rand Zaza, Nazneen Aslam, Hanan Jafar, Suzan Zalloum, Renata Atoom, Abdalla Awidi
Summary: This study investigates the effect of Biodentine(TM) on odonto/osteogenic differentiation potential of hDPSCs in vitro. The results show that treatment with BD significantly increases the expression of odonto/osteogenic markers, upregulates genes related to the odonto/osteogenesis pathway, and enhances calcium deposits, suggesting BD as a promising candidate for regenerative medicine in dental tissue regeneration.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
So Mi Jeon, Je Sun Lim, Hyung-Ryong Kim, Jong-Ho Lee
Summary: The study demonstrates that aerobic glycolysis is induced during odontoblastic differentiation from human DPSCs, with upregulation of protein expression levels of PFKM and PFK2 mainly mediated by AKT activation. This increased PFK activity is essential for enhancing aerobic glycolysis, which plays a crucial role in odontoblastic differentiation of human DPSCs. These findings suggest that PFK activation-induced aerobic glycolysis accompanies and participates in the differentiation of human DPSCs into odontogenic lineage, potentially influencing dental pulp repair.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Cell Biology
Ashraf Al Madhoun, Sardar Sindhu, Dania Haddad, Maher Atari, Rasheed Ahmad, Fahd Al-Mulla
Summary: Dental pulp stem cells have promising characteristics, but genotypic and phenotypic heterogeneities may impact their therapeutic potentials. Studies suggest that their heterogeneity arises from an interplay between intrinsic and extrinsic factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Francine Lorencetti da Silva, Giuliana de Campos Chaves Lamarque, Fernanda Maria Machado Pereira Cabral de Oliveira, Paulo Nelson-Filho, Lea Assed Bezerra da Silva, Raquel Assed Bezerra Segato, Lucia Helena Faccioli, Francisco Wanderley Garcia Paula-Silva
Summary: The study found that LTB4 can promote the differentiation of dental pulp stem cells and mineralization of teeth by regulating the expression of Ibsp and Runx2 genes. Microspheres loaded with LTB4 can continuously release LTB4 in long-term culture and achieve the differentiation and mineralization of dental pulp stem cells.
Article
Biochemistry & Molecular Biology
Huong Thu Vu, Mi-Ran Han, Jun-Haeng Lee, Jong-Soo Kim, Ji-Sun Shin, Ji-Young Yoon, Jeong-Hui Park, Khandmaa Dashnyam, Jonathan Campbell Knowles, Hae-Hyoung Lee, Jong-Bin Kim, Jung-Hwan Lee
Summary: This study investigates the potential of conditioned media derived from stem cells of human exfoliated deciduous teeth (SHED CM) on the regeneration of dental pulp stem cells (DPSCs). The results demonstrate that SHED CM promotes the proliferation, survival, migration, and odontoblastic differentiation of DPSCs. The analysis of SHED CM reveals the presence of various cytokines and growth factors that positively regulate cell function. These findings suggest that SHED CM could be beneficial for pulp regeneration.
Article
Cell Biology
Ning Wang, Xiao Han, Haoqing Yang, Dengsheng Xia, Zhipeng Fan
Summary: The study demonstrates that miR-6807-5p promotes odontogenic differentiation of dental pulp stem cells by silencing METTL7A, while METTL7A promotes odontogenic differentiation of dental pulp stem cells by binding with SNRNP200.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Xiaoliang Wang, Jin Zhang, Zhixin Sun, Sainan Zhao, Wenjuan Deng
Summary: This study aimed to investigate the regulatory effect of miRNA-15b-5p on the differentiation of hDPSCs. The expression of miR-15b-5p was determined in odontoblasts by qRT-PCR. The downstream target gene of miR-15b-5p was confirmed using the DLR assay. Transfection of miR-15b-5p mimic, inhibitor or si-IGF1 into differentiated cells was performed to evaluate the effects on differentiation-related markers.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Cell & Tissue Engineering
Xinghong Luo, Weiqing Feng, Shijiang Huang, Shenghong Miao, Tao Jiang, Qian Lei, Jingyao Yin, Sheng Zhang, Xiaochun Bai, Chunbo Hao, Weizhong Li, Dandan Ma
Summary: mTORC1 activation inhibits odontoblastic differentiation of dental pulp stem cells by suppressing exosome release from odontoblasts. These findings provide new insights into dental pulp complex regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Dentistry, Oral Surgery & Medicine
S. H. Nam, A. Yamano, J. A. Kim, J. Lim, S. H. Baek, J. E. Kim, T. G. Kwon, Y. Saito, T. Teruya, S. Y. Choi, Y. K. Kim, Y. C. Bae, H. I. Shin, J. T. Woo, E. K. Park
Summary: The study identified that INB has a significant role in promoting odontoblast differentiation, tooth root, and dentine formation through the MAP kinase and AKT signaling pathways. The findings suggest that nymphaeols could stimulate the repair processes for dentine defects or injuries.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Polymer Science
Raul Rosales-Ibanez, Nieves Cubo-Mateo, Amairany Rodriguez-Navarrete, Arely M. Gonzalez-Gonzalez, Tomas E. Villamar-Duque, Leticia O. Flores-Sanchez, Luis M. Rodriguez-Lorenzo
Summary: The study aimed to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. The results of cell viability tests, von Kossa and Alizarin red staining demonstrate the printed scaffolds' ability to support DPSCs attachment, proliferation, differentiation and mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be used for further modeling and preclinical experiments in bone engineering studies.
Review
Biochemistry & Molecular Biology
Tomasz Staniowski, Anna Zawadzka-Knefel, Katarzyna Skoskiewicz-Malinowska
Summary: Stem cells are undifferentiated cells capable of self-renewal, proliferation, and differentiation, with potential applications in tissue regeneration. Recent interest in obtaining stem cells from human teeth for biomedical applications has grown, particularly in utilizing Dental Pulp Stem Cells (DPSCs) for various therapeutic possibilities. In vivo studies have shown that DPSCs can differentiate into various tissues, offering hope for regenerative therapy and immunotherapy.
Article
Biochemistry & Molecular Biology
Simona Delle Monache, Fanny Pulcini, Francesca Santilli, Stefano Martellucci, Costantino Santacroce, Jessica Fabrizi, Adriano Angelucci, Maurizio Sorice, Vincenzo Mattei
Summary: Studies suggest that hypoxia treatment of DPSCs promotes their differentiation into neuronal cells in a time-dependent manner. Additionally, the conditioned media from hypoxia-stimulated DPSCs can induce neural differentiation in other neural cells.
Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yanqiu Wang, Xiyao Pang, Jintao Wu, Lin Jin, Yan Yu, Romila Gobin, Jinhua Yu
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Cell Biology
Yanqiu Wang, Yadie Lu, Zehan Li, Yixiang Zhou, Yongchun Gu, Xiyao Pang, Jintao Wu, Romila Gobin, Jinhua Yu
CELL PROLIFERATION
(2018)
Article
Cell Biology
Zehan Li, Ming Yan, Yan Yu, Yanqiu Wang, Gang Lei, Yin Pan, Na Li, Romila Gobin, Jinhua Yu
CELL DEATH & DISEASE
(2019)
Article
Cell & Tissue Engineering
Jintao Wu, Na Li, Yuan Fan, Yanqiu Wang, Yongchun Gu, Zehan Li, Yin Pan, Gobin Romila, Zuomin Zhou, Jinhua Yu
STEM CELLS INTERNATIONAL
(2019)
Article
Biotechnology & Applied Microbiology
Yanping Wang, Yanqiu Wang, Yadie Lu, Jinhua Yu
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Biochemistry & Molecular Biology
Miao Yu, Weili Shen, Xinzhan Shi, Qiong Wang, Lifang Zhu, Xiaohui Xu, Jinhua Yu, Laikui Liu
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Engineering, Biomedical
Zhou Zhou, Xingyun Ge, Minxia Bian, Tao Xu, Na Li, Jiamin Lu, Jinhua Yu
BIOMEDICAL ENGINEERING ONLINE
(2020)
Article
Biochemistry & Molecular Biology
Tao Xu, Xiao Wu, Zhou Zhou, Yu Ye, Chaoting Yan, Nanshan Zhuge, Jinhua Yu
Article
Cell & Tissue Engineering
Xingyun Ge, Zehan Li, Zhou Zhou, Yibo Xia, Minxia Bian, Jinhua Yu
STEM CELL RESEARCH & THERAPY
(2020)
Article
Cell & Tissue Engineering
Gang Lei, Yanqiu Wang, Yan Yu, Zehan Li, Jiamin Lu, Xingyun Ge, Na Li, Ana Gloria Cuba Manduca, Jinhua Yu
STEM CELLS INTERNATIONAL
(2020)
Article
Dentistry, Oral Surgery & Medicine
Yu Ye, Lin Fu, Liu Liu, Tong Xiao, Ana Gloria Cuba Manduca, Jinhua Yu
Summary: This study investigated the impact of hypoxia on human periodontal ligament stem cells (PDLSCs) using RNA sequencing. The expression profiles of circRNAs, lncRNAs, and miRNAs were found to be significantly altered in PDLSCs cultured in low oxygen conditions. Specific circRNAs and lncRNAs may have a competitive role in the differentiation of PDLSCs.
Article
Cell & Tissue Engineering
Yu Ye, Yue Ke, Liu Liu, Tong Xiao, Jinhua Yu
Summary: The study showed that circFAT1 acts as a miRNA sponge in promoting osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) by targeting SMAD5, suggesting it as a potential target for periodontal bone regeneration.
STEM CELLS INTERNATIONAL
(2021)
Article
Cell & Tissue Engineering
Lin Fu, Na Li, Yu Ye, Xiaying Ye, Tong Xiao, Xiao Wu, Yanxia Ma, Jinhua Yu
Summary: Let-7 miRNA family plays a crucial role in regulating the biological features of mesenchymal stem cells. In this study, it was found that hsa-let-7b downregulated after mineralization induction of PDLSCs and negatively impacted their osteogenic differentiation by regulating CTHRC1.
STEM CELLS INTERNATIONAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Rong Guo, Tingjie Gu, Ya Xiao, Tong Xiao, Qian Liu, Zehan Li, Jinhua Yu
Summary: This study investigates the role of miR-27b-5p in regulating osteogenic and odontogenic differentiation of SHEDs and the mechanism involving BMPR1A. The findings suggest that miR-27b-5p in SHEDs/EVs suppresses differentiation and maintains pluripotency partly by targeting BMPR1A-mediated BMP signaling. Inhibition of miR-27b-5p could be a potential strategy for promoting bone and dentin formation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Cell & Tissue Engineering
Yuzhi Li, Minxia Bian, Zhou Zhou, Xiao Wu, Xingyun Ge, Tong Xiao, Jinhua Yu
STEM CELL RESEARCH & THERAPY
(2020)
