Article
Biotechnology & Applied Microbiology
Jerome F. L. Duval, Angelina Razafitianamaharavo, Isabelle Bihannic, Marc Offroy, Nicolas Lesniewska, Benedicte Sohm, Helene Le Cordier, Christian Mustin, Christophe Pagnout, Audrey Beaussart
Summary: The variation of cell-wall ultrastructure, composition, and nanomechanics of Chlorella vulgaris upon culture ageing and lysozyme treatment was investigated using a combination of atomic force microscopy (AFM) and confocal microscopy. The presence of a fibrillated mesh at the surface of cells harvested in the stationary phase was observed through AFM imaging. The structured network formed by fibrils containing the N-acetyl-D-glucosamine unit was severely damaged by lysozyme treatment. Furthermore, lysozyme was found to affect algal physiology, triggering oxidative stress and changes in cell lipid content.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Multidisciplinary Sciences
Ryusei Yamada, Tran Ngoc Trang, Holger Flechsig, Toshiki Takeda, Noriyuki Kodera, Hiroki Konno
Summary: The N-terminal of annexin V has significant influence on 2D crystal formation, as observed using high-speed atomic force microscopy (HS-AFM). Furthermore, a quick purification method is presented to purify recombinant annexin V without any residual affinity tag after protein purification in approximately 3 hours.
Review
Cell Biology
Yuan Qiu, Chen-Chi Chien, Basile Maroulis, Jiani Bei, Angelo Gaitas, Bin Gong
Summary: This article presents a review of the applications of atomic force microscopy (AFM) and fluidic atomic force microscopy (fluidFM) in single-cell studies. It discusses the use of AFM in studying single cells, extracellular vesicles, colloidal force spectroscopy, and single-cell adhesion measurements. FluidFM, a combination of microfluidics and AFM, allows for biological, pathological, and pharmacological studies on single cells in a liquid environment. The review focuses on the capabilities of fluidFM in measuring adhesion, binding forces, and manipulating single cells, as well as its applications in patch clamping and mass measurements.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alex Evilevitch, Sophia Hohlbauch
Summary: This study investigated the mechanical response of the nucleus during the initial stage of herpesvirus infection by using atomic force microscopy. It was found that the chromatin becomes stiffer while the nuclear lamina becomes softer after the injection of viral DNA, which may contribute to maintaining nuclear integrity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Chemistry, Physical
Mi Li, Lianqing Liu, Tomaso Zambelli
Summary: Fluidic force microscopy (FluidFM), combining AFM with microchanneled cantilevers, allows for force-sensitive nanopipette manipulation under aqueous conditions. It has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements at the micro-/nanoscale, showing great potential in biophysical assays, particularly at the single-cell level.
Article
Biology
Takehiko Ichikawa, Dong Wang, Keisuke Miyazawa, Kazuki Miyata, Masanobu Oshima, Takeshi Fukuma
Summary: Chemical fixatives have been found to potentially affect the nanoscale structures of cell surfaces by inducing aggregation of membrane proteins. However, research in this area has been limited due to the lack of methods for observing cell surface structures at the nanoscale. In this study, a method using atomic force microscopy and a microporous silicon nitride membrane was developed to observe cell surfaces with high resolution. The results showed that commonly used fixatives increased the size of protrusions on the cell surface, indicating the aggregation of membrane proteins caused by the fixatives.
COMMUNICATIONS BIOLOGY
(2022)
Article
Energy & Fuels
Paul C. Hackley, Jolanta Kus, Joao Graciano Mendonca Filho, Andrew D. Czaja, Angeles G. Borrego, Dragana Zivotic, Brett J. Valentine, Javin J. Hatcherian
Summary: This study investigates bituminite in Upper Jurassic Kimmeridge Clay source rock using CLSM and AFM, providing better understanding of its nature and properties. The findings include the composition and fluorescence spectroscopy characteristics of bituminite, as well as the effects of broad ion beam milling on its surface morphology.
INTERNATIONAL JOURNAL OF COAL GEOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ivan Liashkovich, Silvio Terra Stefanello, Reshma Vidyadharan, Gunter Haufe, Alexander Erofeev, Peter Gorelkin, Vasilii Kolmogorov, Caren Rigon Mizdal, Alexander Dulebo, Etmar Bulk, Ian U. Kouzel, Victor Shahin
Summary: This study investigates the inhibitory effects of Pitstop-2 on clathrin-mediated endocytosis (CME) and its effects on small GTPases. It is demonstrated that Pitstop-2 and its derivative RVD-127 can directly bind to small GTPases, preventing their interaction with downstream effectors and disrupting overall cell motility and nucleocytoplasmic transport integrity. These findings broaden the research scope of Pitstop-2 and have important implications in physiology, pathophysiology, and drug development.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Keivan Asadi, Junghoon Yeom, Hanna Cho
Summary: Investigating internal resonance (IR) mechanisms in micro/nanoresonators reveals that intermodal coupling between second and third flexural modes in asymmetric structures provides an optimal condition for strong IR, with high energy transfer to the resonated mode. This study introduces design strategies that can be easily integrated into typical micro/nanoelectromechanical systems, offering potential for paradigm-shifting applications in micro/nanosystems.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Chemistry, Applied
Jiabao Zheng, Chuan-he Tang, Jihong Wu, Ge Ge, Mouming Zhao, Weizheng Sun
Summary: Heteroprotein complexes are formed through electrostatic interactions in a purely aqueous environment. This study investigated the structural and functional properties of soy protein isolate/lysozyme (SPI/LYS) complexes. Electrostatic complexation increased the surface hydrophobicity of the complexes, but it did not decrease the enzyme activity of lysozyme. The complexes exhibited flocculated structures and high dilution and NaCl reduced their size.
Article
Chemistry, Multidisciplinary
Jan Patrick Calupitan, Arnaud Brosseau, Pierre Josse, Clement Cabanetos, Jean Roncali, Remi Metivier, Clemence Allain
Summary: This study investigates the mechanofluorochromic behavior of microcrystals and nanostructures using atomic force microscopy (AFM). It is found that the red light photoluminescence of these materials can be turned on by contact AFM, with the intensity of light emission linearly correlated to the applied force. Recovery is associated with a decrease in emission intensity, which can be reactivated by subsequent contact scans.
ADVANCED MATERIALS INTERFACES
(2022)
Editorial Material
Multidisciplinary Sciences
Telmo O. Paiva, Albertus Viljoen, Yves F. Dufrene
Summary: Advancements in atomic force microscopy (AFM) techniques and methodologies in microbiology have enhanced our understanding of microbial cell surfaces. Recent studies have shown that AFM imaging of cells and membranes at or near molecular resolution enables detailed visualization of membrane-drug interactions.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Adria Botet-Carreras, Manel Bosch Marimon, Ruben Millan-Solsona, Eva Aubets, Carlos J. Ciudad, Veronique Noe, M. Teresa Montero, Oscar Domenech, Jordi H. Borrell
Summary: This study assessed the ability of an engineered liposomal formulation to fuse and function as a nanocarrier in cells. Physicochemical changes on the cell surface and liposome-cell membrane interactions were observed using atomic force microscopy and confocal microscopy. The results confirmed the potential of the engineered liposomal formulation as a nanocarrier.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Physiology
Marie-Theres Wanuske, Dominique Brantschen, Camilla Schinner, Chiara Stuedle, Elias Walter, Matthias Hiermaier, Franziska Vielmuth, Jens Waschke, Volker Spindler
Summary: Dp is crucial for desmosome formation, while not influencing individual cadherin binding properties. Instead, macro-clustering of desmosomal adhesion molecules through Dp is crucial.
Article
Chemistry, Physical
Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori
Summary: The research showed that Acinetobacter sp. Tol 5 has strong adhesion force, mainly achieved through the interaction between the AtaA protein on the cell surface and the surface. This adhesion force increases with the increase of AtaA molecules. Amino acid hydrolysates and deionized water can inhibit the direct interaction between cells and surfaces, respectively.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Respiratory System
Severine Mueller-Mottet, Hans Stricker, Guido Domeninghetti, Andrea Azzola, Thomas Geiser, Markus Schwerzmann, Daniel Weilenmann, Otto Schoch, Jean-Marc Fellrath, Thierry Rochat, Frederic Lador, Maurice Beghetti, Laurent Nicod, John-David Aubert, Vladimir Popov, Rudolf Speich, Stephan Keusch, Elisabeth Hasler, Lars C. Huber, Peter Grendelmeier, Michael Tamm, Silvia Ulrich
Article
Critical Care Medicine
Koshika Yadava, Celine Pattaroni, Anke K. Sichelstiel, Aurelien Trompette, Eva S. Gollwitzer, Olawale Salami, Christophe von Garnier, Laurent P. Nicod, Benjamin J. Marsland
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2016)
Article
Critical Care Medicine
Eric Bernasconi, Celine Pattaroni, Angela Koutsokera, Christophe Pison, Romain Kessler, Christian Benden, Paola M. Soccal, Antoine Magnan, John-David Aubert, Benjamin J. Marsland, Laurent P. Nicod
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2016)
Editorial Material
Cardiac & Cardiovascular Systems
Antonio Valenti, Alessio Casutt, Angela Koutsokera, Jasmine Noetzli, Jean Yannis Perentes, Thorsten Krueger, Marco Pons, Laurent P. Nicod, Alban Lovis
ANNALS OF THORACIC SURGERY
(2016)
Article
Cell Biology
Romain Laurent, Aurelie Nallet, Benoit de Billy, Laurent Obert, Laurence Nicod, Christophe Meyer, Pierre Layrolle, Narcisse Zwetyenga, Florelle Gindraux
CELL AND TISSUE BANKING
(2017)
Article
Respiratory System
Meriem Benmerad, Remy Slama, Karine Botturi, Johanna Claustre, Antoine Roux, Edouard Sage, Martine Reynaud-Gaubert, Carine Gomez, Romain Kessler, Olivier Brugiere, Jean-Francois Mornex, Sacha Mussot, Marcel Dahan, Veronique Boussaud, Isabelle Danner-Boucher, Claire Dromer, Christiane Knoop, Annick Auffray, Johanna Lepeule, Laure Malherbe, Frederik Meleux, Laurent Nicod, Antoine Magnan, Christophe Pison, Valerie Siroux
EUROPEAN RESPIRATORY JOURNAL
(2017)
Article
Respiratory System
Maurizio Bernasconi, Alessio Casutt, Angela Koutsokera, Igor Letovanec, Frederic Tissot, Laurent P. Nicod, Alban Lovis
Article
Respiratory System
Lisa Bretagne, Ibrahima-Dina Diatta, Mohamed Faouzi, Antoine Nobile, Massimo Bongiovanni, Laurent P. Nicod, Romain Lazor
Review
Immunology
Pierre-Joseph Royer, Gustavo Olivera-Botello, Angela Koutsokera, John-David Aubert, Eric Bernasconi, Adrien Tissot, Christophe Pison, Laurent Nicod, Jean-Pierre Boissel, Antoine Magnan
Article
Respiratory System
Christian F. Clarenbach, Laurent P. Nicod, Malcolm Kohler
JOURNAL OF THORACIC DISEASE
(2016)
Article
Dentistry, Oral Surgery & Medicine
A. Louvrier, E. Euvrard, L. Nicod, G. Rolin, F. Gindraux, L. Pazart, C. Houdayer, P. Y. Risold, F. Meyer, C. Meyer
INTERNATIONAL ENDODONTIC JOURNAL
(2018)
Article
Respiratory System
Manuela Funke-Chambour, Andrea Azzola, Dan Adler, Constance Barazzone-Argiroffo, Christian Benden, Annette Boehler, Pierre-Olivier Bridevaux, Martin Brutsche, Christian F. Clarenbach, Katrin Hostettler, Rebekka Kleiner-Finger, Laurent P. Nicod, Paola M. Soccal, Michael Tamm, Thomas Geiser, Romain Lazor
Editorial Material
Respiratory System
Laurent Pierre Nicod
Letter
Critical Care Medicine
Adam Ogna, Maurizio Bernasconi, Bastien Belmondo, Olivier Long, Julien Simons, Nicolas Peguret, Raphael Heinzer, Laurent P. Nicod, Jean Bourhis, Alban Lovis
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2017)
Review
Medicine, General & Internal
Celia von Siebenthal, John-David Aubert, Periklis Mitsakis, Patrick Yerly, John O. Prior, Laurent Pierre Nicod
FRONTIERS IN MEDICINE
(2016)