Article
Environmental Sciences
Lan Ma, Chengmei Zhang, Yu Gui, Tingling Zou, Shuhua Xi, Xiaoying Guo
Summary: Excess intake of fluoride leads to fluorosis, which causes muscle damage. In this study, C2C12 cells were used to investigate the impact of fluoride on muscle development and the role of FGF21/ERK signaling pathway. Fluoride inhibited myogenic development and increased muscle atrophy-related markers. Pre-treatment with FGF21 reversed these effects and alleviated muscle atrophy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Cell Biology
Wenlan Li, Jennifer Trieu, Ronnie Blazev, Benjamin L. Parker, Kate T. Murphy, Kristy Swiderski, Gordon S. Lynch
Summary: Cancer cachexia is a common condition in cancer patients, where loss of skeletal muscle mass affects treatment response and quality of life. Sulforaphane (SFN), a natural antioxidant found in cruciferous vegetables, has been shown to decrease oxidative stress. This study investigated whether SFN could attenuate muscle wasting induced by cancer cells and chemotherapy, and found that SFN was able to prevent muscle atrophy and activate antioxidant signaling pathways.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Li Guan, Ziyi Cao, Ziyue Pan, Chao Zhao, Mengjuan Xue, Fan Yang, Jie Chen
Summary: Sarcopenia, a condition associated with aging, has attracted attention as a significant public health burden globally. Nutritional supplements have been explored as potential tools for managing sarcopenia, but the specific nutrients responsible are still understudied. In this study, the levels of short chain fatty acids (SCFAs) and intestinal flora were examined in elderly sarcopenia subjects and healthy individuals. The effect and underlying mechanism of SCFA on C2C12 cell proliferation were evaluated through cell viability detection, flow cytometry, and transcriptome analysis.
Article
Cell Biology
Olivier Le Bacquer, Kassandra Lanchais, Kristell Combe, Loic Van Den Berghe, Stephane Walrand
Summary: Sarcopenia, age-related muscle loss, is associated with changes in skeletal muscle protein homeostasis, which may be regulated by the endocannabinoid system. The CB1 receptor antagonist rimonabant stimulates protein synthesis independently of CB1, suggesting potential for treating sarcopenia.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Kristy Swiderski, Christopher J. Brock, Jennifer Trieu, Annabel Chee, Savant S. Thakur, Dale M. Baum, Paul Gregorevic, Kate T. Murphy, Gordon S. Lynch
Summary: The dystrophin-glycoprotein complex (DGC) is essential for maintaining muscle fiber integrity and transmission of force. Phosphorylation of dystrophin serine 3059 (S3059) plays a role in regulating muscle fiber size and could potentially be targeted for therapeutic interventions against inflammation-associated muscle wasting. Increased ERK activation, dystrophin S3059 phosphorylation, and stable DGC appear to be interconnected in the regulation of muscle fiber size.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Simona Kranjc Brezar, Ajda Medved, Urska Matkovic, Gregor Sersa, Bostjan Markelc, Tim Bozic, Mihaela Jurdana, Maja Cemazar
Summary: Electrochemotherapy (ECT) is a local ablative therapy used to treat various skin and subcutaneous tumors as well as certain tumors in internal organs. The study aimed to investigate the side effects of ECT on skeletal muscle at the cellular level and determine its impact on myogenesis in a mouse muscle cell line.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Satoko Hayashi, Shotaro Sakata, Shotaro Kawamura, Yukako Tokutake, Shinichi Yonekura
Summary: This study reveals the mechanism by which muscle cells exit the cell cycle after differentiation through the degradation of Id3 by XBP1u.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mi Jang, Jana Scheffold, Lisa Marie Rost, Hyejeong Cheon, Per Bruheim
Summary: This study compared the metabolism of C2C12 myoblasts and myotubes under different culture conditions, revealing that cell status has a greater impact on metabolism than the culture medium, and serum-free culture can alter the metabolic characteristics of skeletal muscle cells.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Ayano Kato, Iori Sakakibara, Taku Fukushima, Kosuke Sugiura, Tomoya Fukawa, Takeshi Nikawa, Takayuki Uchida
Summary: This study analyzed the effect of different glucose concentrations on protein expression in muscle cells and found that low and medium glucose concentrations can enhance glucose utilization and induce myosin expression.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Yi-Xuan Li, Chun-Hung Hsiao, Yung-Fu Chang
Summary: This study aimed to investigate the effect of NAC on arecoline-inhibited myoblast differentiation and its underlying mechanisms. The results showed that NAC attenuated the inhibition of myoblast differentiation caused by arecoline and this effect was mediated by the activation/phosphorylation of ERK1/2.
Article
Biochemistry & Molecular Biology
Ailma O. da Paixao, Anaysa Paola Bolin, Joao G. Silvestre, Alice Cristina Rodrigues
Summary: The saturated fatty acid palmitic acid negatively affects muscle tube diameter, fusion, and metabolism, which may be related to miR-206.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
So-Hyun Jeon, Se-Young Choung
Summary: The study found that oyster hydrolysates TGPN and PNY can inhibit sarcopenia by regulating protein turnover and mitochondrial biogenesis, thus improving muscle strength and mass.
Article
Cell Biology
Philip Barrett, Tom J. Quick, Vivek Mudera, Darren J. Player
Summary: This study aimed to generate and characterize intrafusal bag and chain myotubes from differentiated C2C12 myoblasts using the addition of Neuregulin 1 protein. The results showed that Nrg-1 treatment increased the number of intrafusal bag myotubes and enhanced the expression of the specific transcription factor Egr3. Additionally, Nrg-1 treatment had a proliferative effect during the serum starvation-initiated differentiation phase. Although there was no clear evidence for specific intrafusal development, Nrg-1 treated myotubes exhibited some characteristics of intrafusal fibres, such as increased satellite cell numbers and smaller myonuclear domains.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Physiology
Leonit Kiriaev, Ben D. Perry, David A. Mahns, Peter J. Shortland, Asma Redwan, John W. Morley, Stewart I. Head
Summary: The study showed that high dose minocycline treatment can reduce maximal isometric force production in fast-twitch EDL and impair protein synthesis in C2C12 cultured cells. These findings are significant for future research on the efficacy of minocycline treatment in neuromuscular disorders or muscle atrophy conditions.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cyril Rauch, James Leigh
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2015)
Review
Oncology
Khalid O. Alfarouk, Christian-Martin Stock, Sophie Taylor, Megan Walsh, Abdel Khalig Muddathir, Daniel Verduzco, Adil H. H. Bashir, Osama Y. Mohammed, Gamal O. Elhassan, Salvador Harguindey, Stephan J. Reshkin, Muntaser E. Ibrahim, Cyril Rauch
CANCER CELL INTERNATIONAL
(2015)
Review
Pharmacology & Pharmacy
Sophie Taylor, Enrico Pierluigi Spugnini, Yehuda G. Assaraf, Tommaso Azzarito, Cyril Rauch, Stefano Fais
DRUG RESISTANCE UPDATES
(2015)
Review
Oncology
Megan Walsh, Stefano Fais, Enrico Pierluigi Spugnini, Salvador Harguindey, Tareq Abu Izneid, Licia Scacco, Paula Williams, Cinzia Allegrucci, Cyril Rauch, Ziad Omran
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2015)
Review
Multidisciplinary Sciences
Ziad Omran, Paula Williams, Cyril Rauch
Review
Oncology
Ziad Omran, Paula Scaife, Simon Stewart, Cyril Rauch
SEMINARS IN CANCER BIOLOGY
(2017)
Review
Oncology
Salvador Harguindey, Daniel Stanciu, Jesus Devesa, Khalid Alfarouk, Rosa Angela Cardone, Julian David Polo Orozco, Pablo Devesa, Cyril Rauch, Gorka Orive, Eduardo Anitua, Sebastien Roger, Stephan J. Reshkin
SEMINARS IN CANCER BIOLOGY
(2017)
Article
Biophysics
Ziad Omran, Cyril Rauch
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2014)
Article
Chemistry, Multidisciplinary
Saif Shubber, Driton Vllasaliu, Cyril Rauch, Faron Jordan, Lisbeth Illum, Snjezana Stolnik
PHARMACEUTICAL RESEARCH
(2015)
Article
Multidisciplinary Sciences
Mohammed H. Cherkaoui-Rbati, Stuart W. Paine, Peter Littlewood, Cyril Rauch
Article
Biochemistry & Molecular Biology
Cyril Rauch, Panagiota Kyratzi, Sarah Blott, Sian Bray, Jonathan Wattis
Summary: Current genome-wide association studies (GWAS) are expensive for analyzing small gene effects in complex/omnigenic traits due to the need for a large number of individuals. Genomic informational field theory (GIFT), inspired by field theory in physics, offers a different method that can extract information even with precise phenotype measurements and a small population size.
Article
Multidisciplinary Sciences
Lamyaa Al-dalawi, Stephen P. Dunham, Cyril Rauch
Summary: Pulmonary surfactant phospholipids act as a natural barrier against viral infection and have been proven to have biophysical and immunological roles in the respiratory system. In this study, it was found that treating influenza viruses with lyso-lipids can significantly reduce their infectivity, opening up a new path for merging concepts from 'soft matter physics' and virology.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Biochemistry & Molecular Biology
Cyril Rauch, Mohammed Cherkaoui, Sharon Egan, James Leigh
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2017)
Article
Social Sciences, Biomedical
Simon Stewart, Cyril Rauch
SOCIAL THEORY & HEALTH
(2016)
Article
Biochemistry & Molecular Biology
Cyril Rauch, Mohammed Cherkaoui-Rbati
Review
Biochemistry & Molecular Biology
Kumaresan Ramanathan, Minale Fekadie, Giri Padmanabhan, Henok Gulilat
Summary: Long noncoding RNAs (lncRNAs) play critical roles in kidney disease development and their dysregulation can lead to various disease processes. Understanding the function and potential application of lncRNAs in kidney disease may provide new diagnostic and therapeutic opportunities. This review provides an overview of lncRNA characteristics, function, and specific studies related to kidney disease treatment.
CELL BIOCHEMISTRY AND FUNCTION
(2024)