期刊
CELL AND TISSUE RESEARCH
卷 352, 期 2, 页码 237-247出版社
SPRINGER
DOI: 10.1007/s00441-013-1564-3
关键词
Colony circularity; Colony formation; Morphology; Colony-forming unit-fibroblastic assay; Colony growth dynamics; Cell growth potential; Mesenchymal stem cell; Human
类别
资金
- BBSRC [BB/G010579/1]
- BBSRC [BB/G010579/1] Funding Source: UKRI
- MRC [G0802397] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G010579/1] Funding Source: researchfish
- Medical Research Council [G0802397] Funding Source: researchfish
Mesenchymal stem cells (MSCs) provide an ideal cell source for bone tissue engineering strategies. However, bone marrow stromal cell (BMSC) populations that contain MSCs are highly heterogeneous expressing a wide variety of proliferative and differentiation potentials. Current MSC isolation methods employing magnetic-activated and fluorescent-activated cell sorting can be expensive and time consuming and, in the absence of specific MSC markers, fail to generate homogeneous populations. We have investigated the potential of various colony morphology descriptors to provide correlations with cell growth potential. Density-independent colony forming unit-fibroblastic (CFU-F) capacity is a MSC prerequisite and resultant colonies display an array of shapes and sizes that might be representative of cell function. Parent colonies were initially categorised according to their diameter and cell density and grouped before passage for the subsequent assessment of progeny colonies. Whereas significant morphological differences between distinct parent populations indicated a correlation with immunophenotype, enhanced CFU-F capacity was not observed when individual colonies were isolated according to these morphological parameters. Colony circularity, an alternative morphological measure, displayed a strong correlation with subsequent cell growth potential. The current study indicates the potential of morphological descriptors for predicting cell growth rate and suggests new directions for research into dissection of human BMSC CFU-F populations.
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