Review
Physiology
Mian Muhammad Awais, Muhammad Shakeel, Jingchen Sun
Summary: miRNAs are small non-coding RNAs of about 22 nucleotides that have been reported to regulate gene expression at the posttranscriptional level, involved in various biological processes such as immunity, development, metabolism, and host-pathogen interactions. Both cellular and viral miRNAs may play vital roles in host-pathogen interactions, with viral infections altering host miRNA expression profiles.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jing Xu, Xiaole Xie, Qiuqin Ma, Lu Zhang, Yu Li, Yin Chen, Kang Li, Yang Xiao, Gianluca Tettamanti, Hanhong Xu, Ling Tian
Summary: This study characterized the molecules involved in Bombyx mori nucleopolyhedrovirus (BmNPV)-silkworm interaction by RNA sequencing. The upregulated genes were mainly involved in translation, signal transduction, folding, sorting, and degradation, while the downregulated genes were predominantly related to metabolism. Knockout of specific genes inhibited or promoted BmNPV proliferation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xianghan Mei, Chun Li, Peilin Peng, Jue Wang, Enxi He, Zhiyong Qiu, Dingguo Xia, Qiaoling Zhao, Dongxu Shen
Summary: In this study, a C-type lectin gene called BmIML-2 was identified from the silkworm Bombyx mori. It was found that BmIML-2 can inhibit the proliferation of the BmNPV virus by facilitating cell apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Su Lin, Hao Tong Yin, Zhi Meng Zhao, Zi Kang Chen, Xue MIng Zhou, Zheng Dong Zhang, Xi Jie Guo, Wei Guo Zhao, Ping Wu
Summary: In this study, it was found that lincRNA_XR209691.3, a long non-coding RNA, was significantly up-regulated in the silkworm fat body upon BmNPV infection. Further investigation revealed that lincRNA_XR209691.3 interacted with the host protein BmHSP70, promoting the replication and expression of BmNPV. These findings shed light on the role of lncRNAs in insect-pathogen interactions and provide new insights into the molecular mechanism of BmNPV infection.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Entomology
Zhihao Su, Chunxiao Zhao, Xinming Huang, Junli Lv, Ziqin Zhao, Kaiyi Zheng, Xia Sun, Sheng Qin, Xueyang Wang, Byung-Rae Jin, Yangchun Wu
Summary: This study found that the hormone receptor BmEcR-B1 in silkworms is more critical than BmEcR-A in responding to BmNPV infection. Further investigation confirmed that BmEcR-B1 plays an anti-BmNPV role by participating in 20E-induced apoptosis. This study provides theoretical support for understanding the resistant mechanism of silkworms to BmNPV and controlling BmNPV disease.
Article
Multidisciplinary Sciences
Ryoma Tsuchiya, Aino Kaneshima, Masakazu Kobayashi, Maki Yamazaki, Yoko Takasu, Hideki Sezutsu, Yoshiaki Tanaka, Akira Mizoguchi, Kunihiro Shiomi
Summary: Diapause is a major developmental switch in insects evolved as a survival strategy under unfavorable environmental conditions. In the silkworm Bombyx mori, diapause is transgenerationally induced as a maternal effect, with progeny diapause determined by the mother's embryonic environmental temperature. The molecular mechanisms of diapause induction involve a hierarchical pathway regulated by gamma-aminobutyric acid (GABA)ergic and corazonin signaling systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Entomology
Jie Wang, Lin-Bao Zhu, Yan Ma, Ying-Xue Liu, Hui-Hua Cao, Yu-Ling Wang, Xue Kong, Zhi-Hao Huang, Han-Dan Zhu, Yan-Xiang Wang, Shi-Huo Liu, Jia-Ping Xu
Summary: This study identified a differentially expressed beta-1,3-glucan recognition protein, Bm beta GRP4, in response to BmNPV infection and investigated its role in the antiviral defense mechanisms of Bombyx mori. The findings suggest that Bm beta GRP4 may play a crucial role in modulating BmNPV proliferation and apoptosis in B. mori, providing insights into the host-pathogen interactions in insects.
Article
Immunology
Mengmeng Zhang, Shigang Fei, Junming Xia, Yeyuan Wang, Hongyun Wu, Xian Li, Yiyao Guo, Luc Swevers, Jingchen Sun, Min Feng
Summary: This study is the first to investigate the role of Sirtuins in insect-virus interactions. It found that silkworm Sirt2 and Sirt5 have antiviral functions against BmNPV, and silkworm Sirt5 enhances host antiviral response by activating the immune pathway mediated by Relish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiamin Yan, Zhao Xue, Haonan Dong, Jiaxin Pang, Huawei Liu, Jing Gong, Qingyou Xia, Yong Hou
Summary: In this study, the expression and regulation pathways of storage protein genes in silkworms were investigated. The results showed that the expression of storage proteins in silkworm larvae was strongly decreased by starvation, and the insulin-like signaling pathway played an important role in the regulation of storage protein expression. The ILS/FoxO signaling pathway responds to nutrient uptake by regulating the expression of storage proteins.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ying-Xue Liu, Lin-Bao Zhu, Zhi-Hao Huang, Hui-Hua Cao, Hai-Zhong Yu, Shi-Huo Liu, Jia-Ping Xu
Summary: BmFerHCH plays an important role in facilitating BmNPV proliferation by suppressing apoptosis through regulating ROS accumulation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Fei Yin, Miaomiao Xiao, Alexander Berestetskiy, Qiongbo Hu
Summary: This study identified transmembrane protein 214 (BmTEME214) and protein transport protein SEC23A isoform X2 (BmSEC23) as potential Destruxin A (DA)-binding proteins and validated their interaction through bio-layer interferometry (BLI), insect two-hybrid (I2H), and RNAi experiments. The results demonstrated strong affinity between DA and BmTEME214 and BmSEC23 proteins, shedding light on the action mechanism of DA.
Article
Biochemistry & Molecular Biology
Megan A. Hahn, Agnes Piecyk, Fatima Jorge, Robert Cerrato, Martin Kalbe, Nolwenn M. Dheilly
Summary: This study demonstrates that helminths have an impact on the gut microbiome of their host. The microbiome of stickleback fish varies depending on ecoevolutionary variables, such as the genotype of the host and parasite, and the composition of the parasite's microbiome. The study also reveals that the association between the microbiome and immune gene expression increases in infected individuals and varies with parasite genotype. Additionally, it shows that the parasite's microbiome is distinct from its host and affects the host's immune response to infection.
Article
Fisheries
Min Zhu, Jun Pan, Mingtian Zhang, Xinyu Tong, Yunshan Zhang, Ziyao Zhang, Zi Liang, Xing Zhang, Xiaolong Hu, Renyu Xue, Guangli Cao, Chengliang Gong
Summary: The study revealed that infection by Bombyx mori cytoplasmic nucleopolyhedrosis virus induces autophagy in the silkworm midgut through regulating mitophagy proliferation. The structural protein VP4 of BmCPV was found to trigger mitophagy pathway, increasing levels of PINK1 and Parkin proteins while decreasing Tom20 levels. Interaction with Tom40 introduced the VP4-induced mitochondrial autophagy.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Xianghan Mei, Peitong Qiao, Hengheng Ma, Siyu Qin, Xia Song, Qiaoling Zhao, Dongxu Shen
Summary: This study focused on the role of BmTsp.A in the apoptotic pathway and found that its overexpression can promote viral infection and replication. It regulates apoptosis through multiple pathways, providing important insights into the pathogenesis of BmNPV and the immune defense mechanism of silkworms.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
WenTao Ye, MinLi Dai, DanDan Bian, QingYu Zhu, Xin Li, HaiNa Sun, FanChi Li, Jing Wei, Bing Li
Summary: This study investigated the adverse effects of sublethal chlorantraniliprole (CAP) exposure on Bombyx mori. Results showed that CAP induced intracellular Ca2+ release in BmN cells and caused mitochondrial dysfunction, autophagy, and apoptosis in the midgut of B. mori.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Shiwang Yu, Abhishek Kumar Awasthi, Wenting Ma, Mingkang Wen, Luigi Di Sarno, Conghua Wen, Jian Li Hao
Summary: China's rapid urban redevelopment generates a significant amount of construction and demolition (C&D) waste, posing environmental challenges. This study evaluates the effectiveness of C&D waste management policies in Nanjing, Shanghai, and Hangzhou, providing scientific-based decision support for sustainable development and economic growth.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Mathematics
S. Lal, S. Kumar, S. K. Mishra, A. K. Awasthi
Summary: This paper introduces a new computation method to solve problems in approximation theory. Based on pseudo-Chebyshev wavelet approximations, the method is described and error bounds of a function are analyzed. An example is provided to demonstrate the accuracy and efficiency of the pseudo-Chebyshev wavelet approximation method and its main results. Four new error bounds related to the generalized Lipschitz class are obtained using the pseudo-Chebyshev wavelet.
CARPATHIAN MATHEMATICAL PUBLICATIONS
(2022)
Article
Computer Science, Interdisciplinary Applications
V. P. Shyaman, A. Sreelakshmi, Ashish Awasthi
Summary: This paper proposes a tailored finite point method to solve the generalized Burgers' equation by utilizing initial conditions and local properties of the solution. The method shows stability and convergence, and achieves good results in numerical experiments.
JOURNAL OF COMPUTATIONAL SCIENCE
(2022)
Review
Agricultural Engineering
Arun Krishna Vuppaladadiyam, Sai Sree Varsha Vuppaladadiyam, Abhishek Awasthi, Abhisek Sahoo, Shazia Rehman, Kamal Kishore Pant, S. Murugavelh, Qing Huang, Edward Anthony, Paul Fennel, Sankar Bhattacharya, Shao-Yuan Leu
Summary: This review provides an overview of recent advancements in biomass pyrolysis, classifying them into conventional, advanced, and emerging approaches, and summarizing the current status for industrial applications. The impact of different pyrolysis techniques on biomass conversion for hydrogen production is evaluated, and future directions for overcoming current shortcomings are discussed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Mathematics, Applied
V. S. Aswin, T. K. Riyasudheen, Ashish Awasthi
Summary: This paper presents a procedure for incorporating the domain decomposition concept into finite difference and differential quadrature methods. Three numerical schemes are formulated for solving reaction-diffusion and convection-diffusion-reaction systems. The physical domain is subdivided and mesh generation is done locally in each subdomain. Numerical schemes are formulated by using suitable combinations of differential quadrature and finite difference methods for spatial and temporal derivative approximations in each subdomain. The solution is first calculated at the subdomain interfaces and then in the subdomains using the estimated pseudo-boundary conditions. Convergence, stability, the effect of grid refinement, and errors are investigated for a two-component reaction-diffusion system. These schemes are also extended to solve the system of Black-Scholes equations for pricing European options in a regime-switching economy.
NUMERICAL ALGORITHMS
(2023)
Editorial Material
Engineering, Environmental
Abhishek Kumar Awasthi
WASTE MANAGEMENT & RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Abhishek Kumar Awasthi, Mrigendra Kumar Awasthi, Saket Mishra, Surendra Sarsaiya, Akhilesh Kumar Pandey
Summary: This study investigates the toxic substances and materials in electronic waste and discusses their potential adverse impacts on the environment and public health through a qualitative assessment approach. The findings show the presence of hazardous substances within standard limits. The study highlights the potential negative effects of improper recycling practices and the potential for converting electronic waste into urban resources through organized and proper waste resource mining.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Agriculture, Multidisciplinary
Arun Kumar Garov, A. K. Awasthi
Summary: In this article, Autoregressive Integrated Moving Average Models are used to forecast the price of rice in India. The model is based on historical time series data and applied to predict the Indian rice price from June 2022 to December 2038. The evaluation of the model is conducted using MAPE and SPSS.
INTERNATIONAL JOURNAL OF AGRICULTURAL AND STATISTICAL SCIENCES
(2023)
Article
Mathematics, Applied
V. P. Shyaman, A. Sreelakshmi, Ashish Awasthi
Summary: The proposed method is based on the tailored finite point method and designed for solving the non-linear Burgers' equation. It enhances the tailored finite point method with appealing features such as cost-effectiveness and rapidity, and it can handle shocks and boundary layers without mesh refinement. The method is constructed based on the local properties of the solution, even when the kinematic viscosity is infinitesimal, and it uses a 4-point stair stencil to form an implicit scheme that handles non-linearity with adequate linearization techniques. The method has been demonstrated to be second-order convergent in space and first-order convergent in time, and its credibility is validated through comparisons with exact solutions and other methods in the literature.
JOURNAL OF DIFFERENCE EQUATIONS AND APPLICATIONS
(2023)
Article
Engineering, Mechanical
V. P. Rabeeb Ali, Ashish Awasthi, Kottakkaran Sooppy Nisar
Summary: The Keller box method is employed to solve the one-phase moving boundary problem with various factors taken into consideration. The proposed method achieves stability and second-order accuracy through convergence analysis. A similarity solution is used to validate the numerical results, which demonstrate good agreement. The effects of different parameters and size-dependent thermal conductivity on the moving boundary position are also investigated.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART E-JOURNAL OF PROCESS MECHANICAL ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
A. K. Awasthi, Harpreet Kaur, Shavej Rachna, Shavej Ali Siddiqui, Homan Emadifar
Summary: The mathematical theory of elasticity is important in studying physical quantities in structural problems, especially when dealing with crack presence. Integral equations play a vital role in solving various problems, including fracture mechanics and design. This study focused on calculating the accurate stress and displacement components for longitudinal crack propagations using Fourier transform and Fredholm integral equations, which were further simplified to decoupled Fredholm integral equations.
MECHANICS OF ADVANCED MATERIALS AND STRUCTURES
(2023)
Article
Physics, Multidisciplinary
V. P. Rabeeb Ali, Ashish Awasthi
Summary: This paper presents a modified Keller box method for the moving boundary problem (MBP) based on the boundary immobilisation technique. The proposed scheme achieves second-order accuracy in both time and space and shows good agreement with the similarity solution.
PRAMANA-JOURNAL OF PHYSICS
(2023)
Article
Multidisciplinary Sciences
A. K. Awasthi, Harpreet Kaur, Rajendra Kumar Tripathi, Masoumeh Khademi, Homan Emadifar
Summary: This study investigates the effects of arterial stenosis on blood flow characteristics and finds that flow resistance increases with the size of stenosis, while wall shear stress decreases with decreasing peripheral layer viscosity.
Article
Geochemistry & Geophysics
Shriniwas Kolpuke, Christopher D. D. Simpson, Feras Abushakra, Abhishek K. K. Awasthi, Omid Reyhanigalangashi, Jacob Pierce, Tuan Luong, Jordan Larson, Drew Taylor, David Braaten, S. Prasad Gogineni
Summary: We developed a high-sensitivity and low-transmit-power airborne ultra wide band (UWB) frequency-modulated continuous-wave (FMCW) radar for snow depth measurements. The radar achieved near-real-time snow thickness maps by carefully designing the hardware to reduce internal reflections and errors, and performing simulations to predict degradations. Additionally, a T-shape Mills-Cross antenna array was developed for a small overlapped footprint. The radar successfully mapped the top and bottom interfaces and density changes of 1.2-2.1 m of snow, with radar estimates closely matching in situ measurements.
IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING
(2022)