期刊
CELL AND TISSUE BANKING
卷 16, 期 2, 页码 249-259出版社
SPRINGER
DOI: 10.1007/s10561-014-9467-4
关键词
Allograft; Homograft; Dermis; Decellularized; Acellular dermal matrix; MatrACELL
Decellularized human dermis has been used for a number of clinical applications including wound healing, soft tissue reconstruction, and sports medicine procedures. A variety of methods exist to prepare this useful class of biomaterial. Here, we describe a decellularization technology (MatrACELL(A (R))) utilizing a non-denaturing anionic detergent, N-Lauroyl sarcosinate, and endonuclease, which was developed to remove potentially immunogenic material while retaining biomechanical properties. Effective decellularization was demonstrated by a residual DNA content of a parts per thousand currency sign4 ng/mg of wet weight which represented > 97 % DNA removal compared to unprocessed dermis. Two millimeter thick MatrACELL processed human acellular dermal matrix (MH-ADM) exhibited average ultimate tensile load to failure of 635.4 +/- A 199.9 N and average suture retention strength of 134.9 +/- A 55.1 N. Using an in vivo mouse skin excisional model, MH-ADM was shown to be biocompatible and capable of supporting cellular and vascular in-growth. Finally, clinical studies of MH-ADM in variety of applications suggest it can be an appropriate scaffold for wound healing, soft tissue reconstruction, and soft tissue augmentation.
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