Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Virology
Mac Crite, Daniel DiMaio
Summary: Intracellular trafficking of human papillomavirus (HPV) during virus entry requires the involvement of cellular protease gamma-secretase, which interacts with the minor capsid protein L2 and facilitates its insertion into the endosomal membrane in order to bind other cellular factors necessary for proper virus trafficking. The TM domain of HPV L2 is crucial for its protrusion into the cytoplasm, and mutational studies have shown that the infectivity of HPV mutants is closely correlated with gamma-secretase binding and stabilization, highlighting the importance of the native TM domain of HPV L2 for virus entry.
Article
Engineering, Biomedical
Qi Tang, Peng Tan, Zhaolai Dai, Tao Wang, Shenrui Xu, Yakun Ding, Junqi Jin, Xin Zhang, Yucheng Zhang, Chenlong Zhou, Zitian Yue, Huiyang Fu, Junshu Yan, Xi Ma
Summary: This study investigates the association between hydrophobicity and cell-penetrating peptide delivery efficiency, and successfully develops chimeric peptides P3I7 and P3L7 with strong antibacterial activity and low cytotoxicity. These peptides can effectively penetrate cells to eliminate intracellular pathogens mainly through endocytosis. The mechanism of membrane destruction makes them fast sterilizers and less prone to drug resistance. In vivo trials confirm their good biocompatibility and antibacterial infection effects, indicating their potential as affordable and effective antimicrobial agents against intracellular bacterial infections.
ACTA BIOMATERIALIA
(2023)
Article
Virology
David Pim, Justyna Broniarczyk, Abida Siddiqa, Paola Massimi, Lawrence Banks
Summary: Previous studies have identified the involvement of sorting nexins 17 and 27, as well as the retromer complex, in HPV infection. This study reveals the significant role of the newly identified retriever complex and provides insight into how both SNXs contribute to retromer and retriever recruitment during infection.
JOURNAL OF VIROLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yuping Wei, Man Zhang, Pengfei Jiao, Xin Zhang, Ganggang Yang, Xia Xu
Summary: A novel positive CPP carrier P9 was developed to enhance PTX translocation and increase intracellular concentration of PTX, leading to a significant decrease in tumor cell viability.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Xuan Yang, Xiaowei Wen, Jie Dai, Yanming Chen, Wanchuan Ding, Jun Wang, Xiang Gu, Xuejin Zhang, Jin Chen, Roy L. Sutliff, Steven R. Emory, Gang Ruan
Summary: This study investigates nanoparticle transport in hard-to-transfect cells using single-particle motion analysis, cell biology studies, and mathematical modeling. The researchers find significant differences in the transport of nanoparticles in bone marrow-derived mesenchymal stem cells (BMSCs) compared to HeLa cells. They also discover that BMSCs use different biological pathways for cellular uptake, vesicular transport, and exocytosis of nanoparticles compared to HeLa cells. A reaction-diffusion-advection model is used to mathematically integrate the steps of cellular transport and predict nanoparticle delivery in BMSCs. This work provides important insights into nanoparticle transport in hard-to-transfect cells and can guide the design of tailored nanoparticle-based delivery systems.
Article
Biochemistry & Molecular Biology
Mayuko Suzuki, Kouta Iwaki, Moeki Kikuchi, Kei Fujiwara, Nobuhide Doi
Summary: The study found that the fusogenic peptide S19 enhances the endosomal escape efficiency of TAT-fused proteins. Mutagenesis analysis showed that the amino acids with high beta-sheet forming propensities in S19 are important for the intracellular uptake of S19-TAT fusion proteins. The appropriate linking position and order of S19 and TAT peptides were also investigated, and both the previous C-terminal S19-TAT tag and the N-terminal TAT-S19 tag promote the cytoplasmic delivery of the fusion protein.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Shixian Lv, Meilyn Sylvestre, Alexander N. Prossnitz, Lucy F. Yang, Suzie H. Pun
Summary: Peptides as promising cancer therapeutics have shown robust antitumor efficacy, but the translation into clinical therapies is hindered by inherent limitations in their structure, hindering intracellular delivery. Strategies to engineer polymeric materials for increased peptide delivery efficiency, especially cytosolic delivery, play a crucial role in potentiating peptide-based cancer therapies. Future opportunities for peptides in cancer treatment lie in the design of polymer nanocarriers for optimized peptide delivery.
Article
Chemistry, Multidisciplinary
Seung-Eun Chong, Jae Hoon Oh, Kyungjin Min, Sohyun Park, Sejong Choi, Joon Hyung Ahn, Dahyun Chun, Hyung Ho Lee, Jaehoon Yu, Yan Lee
Summary: A new vehicle is designed for intracellular delivery of antibodies at nanomolar concentrations by combining Z domain with LK sequences. The delivered IgGs can control targeted intracellular signaling pathway, showing potential for future therapeutic development.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Pharmacology & Pharmacy
Tejinder Singh, Dong Hyun Kang, Tae Wan Kim, Hye Jeong Kong, Jae Sung Ryu, Seob Jeon, Tae Sung Ahn, Dongjun Jeong, Moo Jun Baek, Jungkyun Im
Summary: The octaarginine-oxaliplatin conjugate successfully delivered oxaliplatin into colon cancer cells at low concentrations, showing high antitumor activity in both in vitro and in vivo studies. This strategy may provide a new approach for designing oxaliplatin derivatives with improved therapeutic capabilities.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Rajendra Joshi, Kamal Sweidan, Deepti Jha, Irina Kerkis, Klaus Scheffler, Joern Engelmann
Summary: This study evaluates the performance of crotamine and CyLoP-1-based probes in cellular internalization and MRI imaging. The results show that both crotamine and CyLoP-1 can effectively penetrate cell membranes and accumulate within cells, but their contrast enhancement efficacy in biological samples is limited. CyLoP-1 accumulates better within cells compared to crotamine, but its cellular relaxation rate is slightly elevated.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Hang Yang, Jingjing Xu, Yujing Gong, Yan Tang, Wuyou Li, Zhenhua Zheng, Yuhong Li, Jin He, Hongping Wei
Summary: A novel internal cell-penetrating peptide was predicted in the chimeric lysin ClyR, allowing it to be internalized by epithelial cells through caveolin-dependent endocytosis for targeting intracellular pathogens. Specific residues in the internal CPP were found to be essential for ClyR-mediated internalization and intracellular killing, providing new insights into the rational designing of next-generation lysins.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Ludger Johannes
Summary: Biological membranes, as stable barriers that delimitate cells, are highly dynamic and enable lateral diffusion, transbilayer passage, and endocytic uptake. Lipids and complex sugars, the fundamental fabrics of membranes, are synthesized through complex biosynthetic enzymes, making them challenging to study using conventional reverse genetics. Organic synthesis offers new avenues for research in membrane glycobiology and its application in biomedicine.
Article
Pharmacology & Pharmacy
Zhili Yu, Xiaojuan Zhang, Xing Pei, Weiran Cao, Junxiao Ye, Jianxin Wang, Lu Sun, Fei Yu, Jiancheng Wang, Nan Li, Kyuri Lee, Stefan Barth, Victor C. Yang, Huining He
Summary: In this study, antibodies and siRNAs were successfully conjugated using a multifunctional peptide as a linker, allowing for the specific delivery of siRNAs to target tumor cells. The released siRNA entered targeted cells via cellular penetrating peptide, resulting in improved gene silencing efficiency. The designed ARCs achieved approximately 66.7% EGFP downregulation efficiency in nude mice xenografted with the HCT116-EGFP tumor model, demonstrating a prospective choice for safe and efficient siRNA delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Pengwei Zhang, Ivan Rayment, Susan P. Gilbert
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Pengwei Zhang, Wei Dai, Juergen Hahn, Susan P. Gilbert
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Willian O. Castillo-Ordonez, Elvira R. Tamarozzi, Gabriel M. da Silva, Andred F. Aristizabal-Pachon, Elza T. Sakamoto-Hojo, Catarina S. Takahashi, Silvana Giuliatti
NEUROCHEMICAL RESEARCH
(2017)
Article
Cell Biology
Takamasa Inoue, Pengwei Zhang, Wei Zhang, Kylia Goodner-Bingham, Allison Dupzyk, Daniel DiMaio, Billy Tsai
JOURNAL OF CELL BIOLOGY
(2018)
Article
Plant Sciences
Cleydson Breno Rodrigues dos Santos, Ryan da Silva Ramos, Brenda Lorena Sanchez Ortiz, Gabriel Monteiro da Silva, Silvana Giuliatti, Jose Luis Balderas-Lopez, Andres Navarrete, Jose Carlos Tavares Carvalho
JOURNAL OF ETHNOPHARMACOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Josiane Cruz, Rodolfo B. Serafim, Gabriel M. da Silva, Silvana Giuliatti, Joaquin M. C. Rosa, Moyses F. Araujo Neto, Franco H. A. Leite, Carlton A. Taft, Carlos H. T. P. da Silva, Cleydson B. R. Santos
JOURNAL OF MOLECULAR MODELING
(2018)
Article
Virology
Natalie V. Akhrameyeva, Pengwei Zhang, Nao Sugiyama, Samuel M. Behar, Feng Yao
JOURNAL OF VIROLOGY
(2011)
Article
Multidisciplinary Sciences
Pengwei Zhang, Lining Xie, John W. Balliet, Danilo R. Casimiro, Feng Yao
Article
Biochemistry & Molecular Biology
Hady Keita, Cleydson Breno Rodrigues dos Santos, Matheus Merces Ramos, Elias Carvalho Padilha, Rodolfo Bortolozo Serafim, Andres Navarrete Castro, Jesus Rafael Rodriguez Amado, Gabriel Monteiro da Silva, Irlon Maciel Ferreira, Silvana Giuliatti, Jose Carlos Tavares Carvalho
Summary: This study aimed to extract and purify Bixin from Bixa orellana seeds, evaluate its hypoglycemic activity in vivo, and study its selectivity on peroxisome proliferator-activated receptors via molecular docking. The results showed that Bixin effectively reduced glucose levels in diabetic rats and exhibited selectivity on PPAR gamma. Further research is needed to explore Bixin's potential as a treatment for diabetes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Multidisciplinary Sciences
Pengwei Zhang, Ruben Moreno, Paul F. Lambert, Daniel DiMaio
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Microbiology
Jian Xie, Pengwei Zhang, Mac Crite, Daniel DiMaio
Article
Multidisciplinary Sciences
Jian Xie, Pengwei Zhang, Mac Crite, Christina Lindsay, Daniel DiMaio
Summary: A protein modulation screen identified four traptamers that inhibit different steps of HPV entry, allowing for the ordering of trafficking steps during entry into a coherent pathway. The results define the retrograde entry route taken by HPV and show that retromer can play a role in CPP-mediated membrane insertion.