Article
Multidisciplinary Sciences
Dan Liu, Vasilios Tsarouhas, Christos Samakovlis
Summary: Cell competition is a homeostatic mechanism where epithelial cells eliminate neighbors with lower fitness. Activation of the endosomal actin regulator WASH links EGFR and Hippo signaling, leading to overgrowth of polarity-deficient cells. The phosphorylation of WASH affects the recycling routes of EGFR and its inhibitor Ptp10D, contributing to epithelial tumorous growth.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jennifer L. Johnson, Elsa Meneses-Salas, Mahalakshmi Ramadass, Jlenia Monfregola, Farhana Rahman, Raquel Carvalho Gontijo, William B. Kiosses, Kersi Pestonjamasp, Dale Allen, Jinzhong Zhang, Douglas G. Osborne, Yanfang Peipei Zhu, Nathan Wineinger, Kasra Askari, Danni Chen, Juan Yu, Scott C. Henderson, Catherine C. Hedrick, Matilde Valeria Ursini, Sergio Grinstein, Daniel D. Billadeau, Sergio D. Catz
Summary: This study investigates the process of exocytosis in neutrophil leukocytes and shows that the actin nucleator protein WASH plays a critical role in this process. The authors find that WASH facilitates the release of gelatinase granules while inhibiting the release of pro-inflammatory granules. They also discover that a deficiency in WASH leads to dysregulated secretion and inflammation in neutrophil leukocytes.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Zehui Lei, Jing Wang, Lingqiang Zhang, Cui Hua Liu
Summary: Membrane trafficking relies on the regulation of small GTPases, which can be modulated through post-translational modifications such as ubiquitination. Dysregulation of small GTPase ubiquitination may lead to various human diseases, highlighting the importance of understanding the molecular mechanisms involved.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Yi-Ting Wang, Guang-Chao Chen
Summary: This study revealed the critical role of ATG9A ubiquitination in oxidative stress-induced autophagy, endocytic trafficking, and innate immunity. TRAF6 mediates ATG9A ubiquitination, promoting autophagy activation, while TNFAIP3/A20 acts as a negative regulator of oxidative stress-induced autophagy.
Article
Multidisciplinary Sciences
Davia Prischich, Nuria Camarero, Javier Encinar del Dedo, Maria Cambra-Pelleja, Judit Prat, Laura Nevola, Andres Martin-Quiros, Elena Rebollo, Laura Pastor, Ernest Giralt, Maria Isabel Geli, Pau Gorostiza
Summary: Clathrin-mediated endocytosis is a common cellular process in eukaryotes. TL2, a light-regulated peptide inhibitor, can precisely control endocytosis. Interestingly, TL2 retains its inhibitory activity in yeast, even though the corresponding target is not conserved in yeast.
Article
Biochemistry & Molecular Biology
Naijin Zhang, Ying Zhang, Boquan Wu, Shaojun Wu, Shilong You, Saien Lu, Jingwei Liu, Xinyue Huang, Jiaqi Xu, Liu Cao, Yingxian Sun
Summary: This study revealed the important role of SIRT2 and PARP1 in regulating oxidative stress-induced vascular injury. SIRT2 interacted with and deacetylated PARP1, leading to its ubiquitination and degradation, ultimately alleviating vascular damage caused by oxidative stress.
Review
Plant Sciences
Min Diao, Shanjin Huang
Summary: Cell-to-cell communication in plants is mediated by plasmodesmata with regulated permeability. Actin cytoskeleton is involved in regulating the permeability, with recent studies on PD-localized formin proteins shedding light on their role and mechanism in intercellular trafficking. This mini-review article describes the progress in understanding these mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sumaya Parveen, Abidur Rahman
Summary: The study demonstrates the important role of the vegetative class actin isovariant ACT7 in regulating the response of Arabidopsis roots to moderate to high temperatures. Loss of ACT7 resulted in increased inhibition of root elongation, reduced cell production rate and cell elongation, and altered dynamics of actin in response to prolonged moderate to high temperatures. This suggests that ACT7 modulates the long-term response of Arabidopsis roots to moderate to high temperatures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
T. Essock-Burns, B. D. Bennett, D. Arencibia, S. Moriano-Gutierrez, M. Medeiros, M. J. McFall-Ngai, E. G. Ruby
Summary: This study demonstrates that quorum-sensing regulation by the Vibrio fischeri population induces a tissue phenotype that promotes the retention of this extracellular symbiont within the light organ of its host. Actin polymerization is identified as the primary mechanism underlying constriction, and host responses to the presence of symbionts change as a function of tissue maturation.
Article
Biochemistry & Molecular Biology
Jaeeun Jung, Jiseok Baek, Kun Tae, Donghyuk Shin, Seungsu Han, Wonjin Yang, Wookyung Yu, Su Myung Jung, Seok Hee Park, Cheol Yong Choi, Sangho Lee
Summary: The study reveals the molecular mechanisms underlying the regulation of Rab7 by site-specific monoubiquitination, where ubiquitination on K191 and K194 plays a crucial role in membrane localization and interaction with SARS-CoV-2. This highlights the common regulatory effects of Rab proteins through site-specific monoubiquitination while emphasizing the specific roles of different ubiquitination sites in each Rab protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Shuichi Takeda, Ryotaro Koike, Ikuko Fujiwara, Akihiro Narita, Makoto Miyata, Motonori Ota, Yuichiro Maeda
Summary: Twinfilin interacts with actin capping protein (CP) in a unique way, regulating CP structure distinct from CARMIL. TWtail and CARMIL CPI restrict CP in different conformations, indicating their unique binding modes with CP.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Vani Pande, Nivedita Mitra, Saket Rahul Bagde, Ramanujam Srinivasan, Pananghat Gayathri
Summary: This study provides insights into the effect of nucleotide state on membrane binding of Spiro plasma citri MreB5 (ScMreB5) and the allosteric effect of Glu134 mutation and the bound nucleotide on membrane binding. The study suggests that MreB protein modulates bacterial morphology by controlling polymerization and disassembly.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lina Lorenzen, Dennis Frank, Carsten Schwan, Robert Grosse
Summary: FMNL2 is upregulated in several cancers and plays important roles in cell migration, invasion, cell-cell adhesion and filopodia formation. Using structured illumination microscopy, it was found that FMNL2 promotes rapid and highly dynamic filopodia formation in epithelial cells while remaining on the tip of the growing filopodia. This filopodia tip localization depends on its N-terminal myristoylation and serine 1072 phosphorylation within the diaphanous-autoregulatory domain (DAD) by protein kinase C (PKC) alpha.
Review
Cell Biology
Elias T. Spiliotis, Ilona A. Kesisova
Summary: This study reviews the mechanism of intracellular long-range transport of membrane vesicles and organelles by microtubule motors. It highlights the spatial control of microtubule-dependent membrane traffic by septins, a unique family of GTPases, and how they regulate motor interactions with microtubules and membrane cargo for specific movement and directionality.
TRENDS IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sungmin Lee, Hyunkoo Kang, Eunguk Shin, Jaewan Jeon, HyeSook Youn, BuHyun Youn
Summary: The study revealed the molecular mechanism underlying GBM progression after radiotherapy, showing that upregulated expression of BEX1 and BEX4 is associated with activation of the YAP/TAZ signaling pathway. Additionally, latrunculin B can inhibit GBM progression by suppressing actin polymerization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Lejiao Mao, Chenyi Liao, Jiao Qin, Yanqiu Gong, Yifei Zhou, Shasha Li, Zhe Liu, Huaqing Deng, Wankun Deng, Qingxiang Sun, Xianming Mo, Yu Xue, Daniel D. Billadeau, Lunzhi Dai, Guohui Li, Da Jia
Summary: This study reveals that external stimuli such as starvation, LPS, and inflammatory cytokines can inhibit endocytic recycling of proteins by activating MAPK11/14, leading to a disruption in the balance between recycling and degradation. Stress-induced kinases phosphorylate SNX27 at Ser51, altering its binding with cargo proteins and thereby suppressing endocytic recycling. The findings suggest that cells can modulate endocytic recycling in response to stress signals, providing new insights into how cells cope with stress and conserve energy.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tuo Li, Tuozhi Huang, Mingjian Du, Xiang Chen, Fenghe Du, Junyao Ren, Zhijian J. Chen
Summary: The study found that cGAS activity is selectively suppressed during mitosis in human cell lines due to hyperphosphorylation at the N terminus by mitotic kinases and prevention of oligomerization of chromatin-bound cGAS, ensuring its inactivity during mitosis when associated with chromatin.
Review
Cell Biology
Xin Yong, Lejiao Mao, Xiaofei Shen, Zhen Zhang, Daniel D. Billadeau, Da Jia
Summary: Endosomes are crucial cellular stations for protein trafficking, where proteins can be degraded or recycled to different cellular destinations. Recent studies have shown that pathogens like bacteria and viruses exploit host endosomal recycling pathways for their survival and replication. This manipulation of host signaling pathways by pathogens deepens our understanding of the molecular intricacies regulating endosomal trafficking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Min Sui, Meimei Xiong, Yuling Li, Qiao Zhou, Xiaofei Shen, Da Jia, Maling Gou, Qingxiang Sun
Summary: Peptides can be designed as non-covalent inhibitors for molecularly targeted therapy, and nanoparticle-mediated gene expression systems can efficiently deliver peptides into cancer cells for improved therapeutic efficacy. This method showed effectiveness against cancer cells in experiments and may provide a new approach for the development of targeted therapy in the future.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Medicinal
Yuqin Lei, Qi An, Xiao-Fei Shen, Min Sui, Chungen Li, Da Jia, Youfu Luo, Qingxiang Sun
Summary: The research team successfully designed a noncovalent CRM1 inhibitor with high affinity for human and yeast CRM1 through crystal structure studies, showing superior properties compared to covalent inhibitors. This discovery opens up new avenues for the development of more effective, less toxic, and broad-spectrum anticancer/antiviral therapies.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Plant Sciences
Yuqin Lei, Yuling Li, Yuping Tan, Zhiyong Qian, Qiao Zhou, Da Jia, Qingxiang Sun
Summary: Plant-derived CRM1 inhibitors plumbagin and oridonin inhibit CRM1 activity through multiple mechanisms, including directly targeting the NES groove and promoting CRM1 aggregation. The oridonin complex reveals for the first time a more open NES groove structure. These findings may provide new strategies for the development of CRM1 inhibitors.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Lvqin Zheng, Zhe Liu, Yan Wang, Fan Yang, Jinrui Wang, Wenjie Huang, Jiao Qin, Min Tian, Xiaotang Cai, Xiaohui Liu, Xianming Mo, Ning Gao, Da Jia
Summary: The structural elucidation and functional analysis of the human GMPPA-GMPPB complex demonstrates how GMPPA acts as a 'sensor' to regulate GMPPB activity and maintain cellular GDP-mannose homeostasis through allosteric mechanisms. This study reveals that GMPPA has a higher affinity to GDP-mannose compared to GMPPB, and can inhibit the catalytic activity of GMPPB. Disruption of GMPPA-GMPPB interactions or GDP-mannose binding to GMPPA leads to abnormal development phenotypes in zebrafish, similar to individuals with GMPPA or GMPPB mutations.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Seoyun Yum, Minghao Li, Yan Fang, Zhijian J. Chen
Summary: This study shows that STING can function independently of type I interferons and autophagy, and that TBK1 recruitment to STING is essential for antiviral and antitumor immunity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Cell Biology
Weizhi Zhang, Zhu Han, Yu Xue, Da Jia
Summary: Macroautophagy/autophagy can selectively degrade misfolded proteins and damaged organelles, potentially contributing to cancer. Mutations in proteins like ATG4B, STBD1, EHMT2, and BRAF disrupt their interactions with LC3 and autophagy activities. The poorly-characterized protein STBD1 inhibits tumor growth through metabolism reprogramming and a patient-derived mutation in STBD1 promotes tumor growth by disrupting the interaction with LC3.
Article
Biochemistry & Molecular Biology
Yuping Tan, Xia Zhou, Yanqiu Gong, Kun Gou, Youfu Luo, Da Jia, Lunzhi Dai, Yinglan Zhao, Qingxiang Sun
Summary: The study identified a new PHGDH inhibitor, oridonin, and revealed the mechanism of its binding to PHGDH structure, providing new insights for the future design of PHGDH inhibitors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Tong Li, Seoyun Yum, Minghao Li, Xiang Chen, Xiaoxia Zuo, Zhijian J. Chen
Summary: Defective DNA clearance in DNase II-/- mice leads to lethal inflammatory diseases that can be rescued by deleting cGAS or STING. The study found that distinct signaling pathways downstream of STING play different roles in disease manifestation, with TBK1 recruitment to STING mediating autoinflammatory arthritis caused by DNase II deficiency. This discovery may provide new insights into therapeutic strategies for certain autoinflammatory diseases.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Yuying Li, Xinmin Yang, Linrui Peng, Qing Xia, Yuwei Zhang, Wei Huang, Tingting Liu, Da Jia
Summary: This study comprehensively summarizes the phenotypes, mechanisms, and treatment options of human diseases caused by BSCL2 gene mutations, as well as relevant findings in animal studies. Restoring adipose tissue function and targeting seipin-related pathways are effective strategies for CGL2 treatment and also have potential therapeutic value in other diseases.
Review
Biochemistry & Molecular Biology
Huan Rui, Kate S. Ashton, Jaeki Min, Connie Wang, Patrick Ryan Potts
Summary: Molecular glues are small molecules that stabilize protein-protein interactions and have promising therapeutic potential. Clinical compounds called molecular glue degraders can stabilize interactions between E3 ubiquitin ligases and target proteins, leading to targeted protein degradation. However, the rational discovery of molecular glues is challenging due to limited understanding of the interactions they stabilize. This review summarizes the structures and interface properties of known molecular glue-induced ternary complexes and discusses computational approaches for predicting protein-protein interfaces, providing valuable insights for future rational molecular glue discovery.
RSC CHEMICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Justin M. Jenson, Tuo Li, Fenghe Du, Chee-Kwee Ea, Zhijian J. Chen
Summary: cGAS is a conserved enzyme that plays a crucial role in immune defense. It is activated by DNA to produce cGAMP, which leads to the expression of antimicrobial genes. In bacteria, a similar enzyme system called CBASS has been discovered, where a protein called Cap2 forms a thioester bond with cGAS and promotes its conjugation to target proteins, increasing cGAMP production.
Review
Chemistry, Multidisciplinary
Robert G. Guenette, Seung Wook Yang, Jaeki Min, Baikang Pei, Patrick Ryan Potts
Summary: Targeted protein degradation strategies, such as PROTAC and molecular glue technology, have provided highly selective control of target inhibition, revolutionizing the approach to challenging protein targets. These advancements have broad implications in treating diseases by expanding the range of possible targets that can be addressed by small molecules.
CHEMICAL SOCIETY REVIEWS
(2022)
