Review
Immunology
Noymar Luque-Campos, Felipe A. Bustamante-Barrientos, Carolina Pradenas, Cynthia Garcia, Maria Jesus Araya, Candice Bohaud, Rafael Contreras-Lopez, Roberto Elizondo-Vega, Farida Djouad, Patricia Luz-Crawford, Ana Maria Vega-Letter
Summary: MSCs, multipotent adult stromal cells, are widely studied for their regenerative and immunomodulatory properties, capable of modulating macrophage plasticity and function. Metabolic changes can also affect macrophage fate and function, prompting phenotype change in response to environmental cues. Understanding the crosstalk between macrophages and MSCs can improve therapeutic potential in inflammatory diseases, cancer, and tissue repair processes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Vincent Fregona, Manon Bayet, Mathieu Bouttier, Laetitia Largeaud, Camille Hamelle, Laura A. Jamrog, Nais Prade, Stephanie Lagarde, Sylvie Hebrard, Isabelle Luquet, Veronique Mansat-De Mas, Marie Nolla, Marlene Pasquet, Christine Didier, Ahmed Amine Khamlichi, Cyril Broccardo, Eric Delabesse, Stephane J. C. Mancini, Bastien Gerby
Summary: The PAX5::ELN oncogenic model disrupts differentiation of preleukemic cells and leads to the emergence of rare and quiescent pre-LSCs that reactivate an immature molecular program similar to chemo-resistant cells in B-ALL.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Pratyusha Bala, Jonathan P. Rennhack, Daulet Aitymbayev, Clare Morris, Sydney M. Moyer, Gina N. Duronio, Paul Doan, Zhixin Li, Xiaoyan Liang, Jason L. Hornick, Matthew B. Yurgelun, William C. Hahn, Nilay S. Sethi
Summary: Cell state plasticity is regulated in adult epithelia to prevent cancer, but its expansion in neoplasia is poorly understood. Mouse models and patient samples revealed that impaired differentiation precedes cancer development, with aberrant transcriptional state marked by Ly6a and reactivation of fetal genes. Genetic inactivation of Sox9 prevents adenoma formation and restores multilineage differentiation. These findings indicate that unabated regenerative activity and developmental reprogramming contribute to the aberrant cell state plasticity preceding cancer development.
Article
Biochemical Research Methods
Jennifer Hammelman, Tulsi Patel, Michael Closser, Hynek Wichterle, David Gifford
Summary: This study compares nine computational methods for identifying reprogramming factors for cell differentiation. The researchers found that methods using chromatin accessibility performed the best, with an average identification rate of 50-60% of reprogramming factors in the top ten candidates. Among the chromatin accessibility methods, DeepAccess and diffTF showed the highest correlation with the ranked significance of transcription factor candidates. AME and diffTF were identified as optimal methods for transcription factor recovery, allowing for systematic prioritization of candidates in the design of new reprogramming protocols.
Editorial Material
Cell Biology
Pooja Popli, Ramakrishna Kommagani
Summary: Autophagy plays a crucial role in the normal growth and morphogenesis of various tissues, including the uterus. Our study reveals that BECN1-dependent autophagy is essential for stem cell-mediated endometrial programming and the establishment of pregnancy in mice. Inhibition of BECN1-mediated autophagy leads to severe endometrial defects and infertility, highlighting the critical role of autophagy in maintaining endometrial homeostasis and uterine differentiation.
Article
Immunology
Jinman Kim, Jaewon Yoon, Jung-Eun Park
Summary: This study investigated the mechanism of cell entry for SADS-CoV virus and found that furin protein is crucial for cell-cell fusion of the virus. Mutagenesis analysis demonstrated the specific cleavage site of furin in the S protein. These findings provide potential targets for the treatment of SADS-CoV.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Endocrinology & Metabolism
Chi Ma, FuKun W. Hoffmann, Michael P. Marciel, Kathleen E. Page, Melodie A. Williams-Aduja, Ellis N. L. Akana, Greg S. Gojanovich, Mariana Gerschenson, Johann Urschitz, Stefan Moisyadi, Vedbar S. Khadka, Sharon Rozovsky, Youping Deng, F. David Horgen, Peter R. Hoffmann
Summary: T cell activation triggers metabolic reprogramming for energy demands and cell proliferation. Ethanolamine phospholipid synthesis plays a crucial role during this process as a regulator of metabolic shifts in stem cells and cancer cells.
MOLECULAR METABOLISM
(2021)
Article
Multidisciplinary Sciences
Andrew Keniry, Natasha Jansz, Linden J. Gearing, Iromi Wanigasuriya, Joseph Chen, Christian M. Nefzger, Peter F. Hickey, Quentin Gouil, Joy Liu, Kelsey A. Breslin, Megan Iminitoff, Tamara Beck, Andres Tapia del Fierro, Lachlan Whitehead, Andrew Jarratt, Sarah A. Kinkel, Phillippa C. Taberlay, Tracy Willson, Miha Pakusch, Matthew E. Ritchie, Douglas J. Hilton, Jose M. Polo, Marnie E. Blewitt
Summary: In this study, a replenishable female mouse embryonic stem cell system was developed to study X chromosome inactivation (XCI). Through a targeted genetic screen, the researchers identified the BAF complex as essential for creating nucleosome-depleted regions at promoters on the inactive X chromosome during the early stages of XCI establishment. This model system provides a valuable tool for discovering unknown factors involved in XCI and epigenetic silencing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Qingnan Tian, Yujia Sun, Tingting Gao, Jiaxin Li, Huimin Fang, Shoutao Zhang
Summary: SUMOylation and ubiquitylation are crucial processes in eukaryotic biology, with Djubc9 and Djnedd4L identified as important factors in head regeneration in planarians through RNA-seq analysis. RNA interference experiments confirmed their roles in stem cell maintenance and regeneration processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Xiao-Jun Li, Charles Morgan, Loyal A. Goff, Angelika Doetzlhofer
Summary: This study found that transient activation of the progenitor-specific RNA binding protein LIN28B and Activin antagonist follistatin (FST) enhances regenerative competence of maturing/mature cochlear supporting cells (SCs) by reprogramming them into progenitor-like cells in organoid culture. LIN28B drives SC reprogramming, while FST is required to counterbalance hyperactivation of transforming growth factor-beta-type signaling by LIN28B.
Article
Cell & Tissue Engineering
Devansh Agarwal, Nicholas Dash, Kevin W. Mazo, Manan Chopra, Maria P. Avila, Amit Patel, Ryan M. Wong, Cairang Jia, Hope Do, Jie Cheng, Colette Chiang, Shawna L. Jurlina, Mona Roshan, Michael W. Perry, Jong M. Rho, Risa Broyer, Cassidy D. Lee, Robert N. Weinreb, Cezar Gavrilovici, Nicholas W. Oesch, Derek S. Welsbie, Karl J. Wahlin
Summary: This study explores cellular reprogramming using transcription factor regulations to convert human pluripotent stem cells into induced neurons similar to retinal ganglion cells. It also demonstrates the potential of small molecule inhibitors to block neuronal death and provides valuable insights for cell replacement and neuroprotection strategies.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biology
Elitsa Stoyanova, Michael Riad, Anjana Rao, Nathaniel Heintz
Summary: At birth, the mammalian brain contains billions of neurons, which develop into networks and learn adult brain tasks through chemical marks such as methylation and hydroxymethylation. Accumulation of 5hmC, possibly essential for neuronal maturation, highlights the role of Tet proteins in preparing and removing DNA marks. Problematic control of 5hmC levels could potentially contribute to brain disorders, while future studies may provide insights into cancerous cell development due to loss of 5hmC.
Article
Biochemistry & Molecular Biology
Yuting Liu, Jiangping He, Ruhai Chen, He Liu, Jocelyn Chen, Yujian Liu, Bo Wang, Lin Guo, Duanqing Pei, Jie Wang, Jing Liu, Jiekai Chen
Summary: The study found that the closing of somatic loci is slower in humans compared to mice, with the AP-1 motif highly enriched among closed loci. The introduction of JDP2 enhances human reprogramming, while known pluripotent factors like ESRRB, KDM2B, and SALL4 fail to increase human iPSC generation. JDP2 promotes the closing of somatic loci enriching AP-1 motifs to enhance human reprogramming.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biology
Joao C. Sabino, Madalena R. de Almeida, Patricia L. Abreu, Ana M. Ferreira, Paulo Caldas, Marco M. Domingues, Nuno C. Santos, Claus M. Azzalin, Ana Rita Grosso, Sergio Fernandes de Almeida
Summary: The activity of TET enzymes promotes the formation of R-loops, revealing new mechanisms of gene expression regulation.
Article
Biochemistry & Molecular Biology
Sergey A. Sinenko, Andrey A. Kuzmin, Elena V. Skvortsova, Sergey V. Ponomartsev, Evgeniya V. Efimova, Michael Bader, Natalia Alenina, Alexey N. Tomilin
Summary: In this study, the researchers investigated the impact of endogenous serotonin on reprogramming to pluripotency and found that serotonin synthesis has a negative effect on somatic cell reprogramming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Helena G. Asenjo, Amador Gallardo, Lourdes Lopez-Onieva, Irene Tejada, Jordi Martorell-Marugan, Pedro Carmona-Saez, David Landeira
Article
Cell Biology
Tom Stiff, Fabio R. Echegaray-Iturra, Harry J. Pink, Alex Herbert, Constantino Carlos Reyes-Aldasoro, Helfrid Hochegger
Article
Nuclear Science & Technology
Makoto I. Kobayashi, Maurizio Angelone, Sachiko Yoshihashi, Kunihiro Ogawa, Mitsutaka Isobe, Takeo Nishitani, Siriyaporn Sangaroon, Shuji Kamio, Yutaka Fujiwara, Tomomi Tsubouchi, Akira Uritani, Minoru Sakama, Masaki Osakabe
FUSION ENGINEERING AND DESIGN
(2020)
Editorial Material
Cell & Tissue Engineering
Tomomi Tsubouchi, Carlos-Filipe Pereira
CELLULAR REPROGRAMMING
(2021)
Article
Biochemistry & Molecular Biology
Sara C. Credendino, Marta De Menna, Irene Cantone, Carmen Moccia, Matteo Esposito, Luigi Di Guida, Mario De Felice, Gabriella De Vita
Summary: FOXE1, a lineage-restricted transcription factor, is involved in thyroid cancer susceptibility by regulating gene expression, affecting cancer development processes such as cell migration and immune cell recruitment. The expression of FOXE1 induces changes in specific chemokines, thus influencing macrophage infiltration in the thyroid cancer microenvironment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Tomas Pachano, Victor Sanchez-Gaya, Thais Ealo, Maria Mariner-Fauli, Tore Bleckwehl, Helena G. Asenjo, Patricia Respuela, Sara Cruz-Molina, Maria Munoz-San Martin, Endika Haro, Wilfred F. J. van IJcken, David Landeira, Alvaro Rada-Iglesias
Summary: Research found that isolated CGIs are crucial for enhancing the long-range regulatory activity of poised enhancers and the interaction with distantly located genes, especially for genes with large CGI clusters in their promoters.
Review
Biochemistry & Molecular Biology
Alessandra Spaziano, Irene Cantone
Summary: Mammalian females silence transcription on one of the X chromosomes to compensate the expression dosage with males. X-chromosome inactivation involves various epigenetic modifications to maintain silencing and make it heritable. Despite genes on the inactive X chromosome being refractory to reactivation, reactivation can occur alongside epigenome reprogramming or by perturbing silencing pathways.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Editorial Material
Ecology
Corinna Ponti, Giuseppe Nasti, Diego Di Girolamo, Irene Cantone, Fahad A. Alharthi, Antonio Abate
Summary: Lead, known as one of the most toxic elements, has been prohibited due to its severe environmental and health impacts. However, as we enter the perovskite era, the widespread application of lead may be necessary once again.
TRENDS IN ECOLOGY & EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Amador Gallardo, Aldara Molina, Helena G. Asenjo, Lourdes Lopez-Onieva, Jordi Martorell-Marugan, Mencia Espinosa-Martinez, Carmen Grinan-Lison, Juan Carlos Alvarez-Perez, Francisca E. Cara, Saul A. Navarro-Marchal, Pedro Carmona-Saez, Pedro P. Medina, Juan Antonio Marchal, Sergio Granados-Principal, Antonio Sanchez-Pozo, David Landeira
Summary: This study reveals a novel role of PRC2 as a master regulator of EMT in carcinoma cells, which has important implications for the design of therapies based on EZH2 inhibitors in human cancer patients.
Article
Multidisciplinary Sciences
Helena G. Asenjo, Maria Alcazar-Fabra, Mencia Espinosa-Martinez, Lourdes Lopez-Onieva, Amador Gallardo, Emilia Dimitrova, Angelika Feldmann, Tomas Pachano, Jordi Martorell-Marugan, Pedro Carmona-Saez, Antonio Sanchez-Pozo, Alvaro Rada-Iglesias, Robert J. Klose, David Landeira
Summary: The ability of pluripotent cells to differentiate is enhanced during the G1 phase of the cell cycle, and this process is regulated by polycomb activity during interphase progression. The recruitment of PRC1 and related molecular functions, such as chromatin interactions, is increased during S and G2 phases compared to the G1 phase. Disruption of PRC1 activity affects the transcriptional repression of developmental regulator genes and interferes with the induction of differentiation genes in G1 phase, suggesting that polycomb repression during interphase transition modulates pluripotent cell differentiation.
NATURE COMMUNICATIONS
(2023)
Biographical-Item
Immunology
Amanda G. G. Fisher, Matthias Merkenschlager
Article
Cell Biology
Joan Domingo-Reines, Rosa Montes, Adrian Garcia-Moreno, Amador Gallardo, Jose Manuel Sanchez-Manas, Ivan Ellson, Mar Lamolda, Chiara Calabro, Jose Antonio Lopez-Escamez, Purificacion Catalina, Pedro Carmona-Saez, Pedro J. Real, David Landeira, Veronica Ramos-Mejia
Summary: Pediatric Acute Myeloid Leukemia (AML) is a rare and heterogeneous disease characterized by gene fusions. Despite improved survival, a relapse still occurs in about 50% of patients. The NUP98-KDM5A chimeric protein is found exclusively in a specific subgroup of pediatric AML patients with poor prognosis. This study reveals that NUP98-KDM5A promotes genomic instability and contributes to malignant transformation.
CELL DEATH & DISEASE
(2023)
Article
Biology
Hannah J. J. Gleneadie, Beatriz Fernandez-Ruiz, Alessandro Sardini, Mathew Van de Pette, Andrew Dimond, Rab K. K. Prinjha, James McGinty, Paul M. W. French, Hakan Bagci, Matthias Merkenschlager, Amanda G. G. Fisher
Summary: Duchenne muscular dystrophy (DMD) is a genetic disorder caused by mutations in the dystrophin gene (Dmd), leading to progressive muscle weakening. A study using mouse models and myoblasts found that chromatin-modifying drugs can increase the expression of utrophin (Utrn), a protein that can compensate for Dmd deficiency. Inhibitors of PRC2 and ERK1/2 were identified to enhance Utrn expression, offering potential therapeutic strategies for DMD.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Guixiang Li, Yong-Tao Liu, Feng Yang, Meng Li, Zuhong Zhang, Jorge Pascual, Zhao-Kui Wang, Shi-Zhe Wei, Xin-Yuan Zhao, Hai-Rui Liu, Jin-Bo Zhao, Chieh-Ting Lin, Jun-Ming Li, Zhe Li, Antonio Abate, Irene Cantone
Summary: This study investigates the biotoxicity of lead, tin, and bismuth perovskites in mice and analyzes their effects on mice and the development of their offspring. The research provides valuable insights into evaluating the environmental and health safety of perovskite materials.
ADVANCED MATERIALS
(2023)
Article
Biology
Amador Gallardo, Aldara Molina, Helena G. Asenjo, Jordi Martorell-Marugan, Rosa Montes, Veronica Ramos-Mejia, Antonio Sanchez-Pozo, Pedro Carmona-Saez, Lourdes Lopez-Onieva, David Landeira
LIFE SCIENCE ALLIANCE
(2020)