Article
Engineering, Biomedical
Hyuna Kim, Anna Wirasaputra, Farnaz Mohammadi, Aritra Nath Kundu, Jennifer A. E. Esteves, Laura M. M. Heiser, Aaron S. S. Meyer, Shelly R. R. Peyton
Summary: Breast cancer is a major cause of cancer-related deaths worldwide, primarily due to metastasis and dormancy. Dormancy, a prolonged period between tumor resection and relapse, poses significant challenges in current therapies. To overcome this, a live cell lineage approach is used to track and distinguish dormant cells from proliferative and dying cells in various in vitro microenvironments. The study reveals that the proportion of long-term quiescent cells is regulated by both intrinsic and extrinsic factors, with the highest percentage found in 3D collagen gels. This approach is expected to benefit the study of dormant tumor cells for biologists and bioengineers in the dormancy community.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Immunology
Jue Feng, Joseph N. Pucella, Geunhyo Jang, Marcela Alcantara-Hernandez, Samik Upadhaya, Nicholas M. Adams, Alireza Khodadadi-Jamayran, Colleen M. Lau, Marlon Stoeckius, Stephanie Hao, Peter Smibert, Aristotelis Tsirigos, Juliana Idoyaga, Boris Reizis
Summary: This study investigates the developmental origins of dendritic cells (DCs) and reveals the shared origin of pDCs and cDCs, suggesting a revised scheme of DC development.
Review
Cell Biology
Anna Konturek-Ciesla, David Bryder
Summary: Appropriate production of mature blood cells is essential for organismal health. This article describes tools and methods for tracing HSC lineage in mice and integrating them with single-cell molecular analysis. By combining new insights from experiments with past knowledge, we gain a better understanding of HSC-derived hematopoiesis in humans.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Chemistry, Analytical
Xiaohua Jia, Guodong Shen, Jia Jia, Yan Zhang, Dan Zhang, Wanjun Li, Jianjun Zhang, Xinglu Huang, Jie Tian
Summary: This article introduces the importance of cancer stem cells and the method of lineage tracing, as well as the application of molecular imaging in the dynamic detection of cancer stem cells. The article analyzes the current situation and looks forward to the future development of cancer stem cell detection.
Editorial Material
Oncology
Eunmi Lee, Yibin Kang
Summary: The combination of single-cell lineage tracing and RNA sequencing has opened up unprecedented opportunities for prospectively exploring metastatic dynamics. In a pancreatic cancer model, Simeonov et al. developed the macsGESTALT lineage recording system, revealing that hybrid EMT states and S100 expression are associated with increased metastatic abilities.
Article
Cardiac & Cardiovascular Systems
Tea Kocijan, Michael Rehman, Andrea Colliva, Elena Groppa, Matteo Leban, Simone Vodret, Nina Volf, Gabriele Zucca, Ambra Cappelletto, Giulia Maria Piperno, Lorena Zentilin, Mauro Giacca, Federica Benvenuti, Bin Zhou, Ralf H. Adams, Serena Zacchigna
Summary: Cardiac ischemia does not effectively induce angiogenesis, leading to cardiomyocyte death and loss of function. Clinical trials attempting to induce therapeutic revascularization through pro-angiogenic molecule delivery have failed, indicating poor angiogenic response in cardiac endothelial cells.
CARDIOVASCULAR RESEARCH
(2021)
Editorial Material
Biochemistry & Molecular Biology
Christina M. J. E. Vandenbroucke-Grauls, Jan A. J. W. Kluytmans
Summary: Antibiotic resistance genes evolve in the environment, animals, and humans, and it is crucial to take strategic action in all three areas to understand and limit resistance.
Article
Biochemistry & Molecular Biology
Theresa B. Loveless, Joseph H. Grotts, Mason W. Schechter, Elmira Forouzmand, Courtney K. Carlson, Bijan S. Agahi, Guohao Liang, Michelle Ficht, Beide Liu, Xiaohui Xie, Chang C. Liu
Summary: CHYRON is a novel DNA recorder that primarily writes new DNA by repeatedly inserting random nucleotides at a single locus in temporal order, instead of erasing DNA like existing recorders. By combining Cas9, a homing guide RNA, and template-independent DNA polymerase, CHYRON has been successfully applied as an evolving lineage tracer and a recorder of user-selected cellular stimuli.
NATURE CHEMICAL BIOLOGY
(2021)
Review
Oncology
Calum Gabbutt, Nicholas A. Wright, Ann-Marie Baker, Darryl Shibata, Trevor A. Graham
Summary: The dynamical process of cell division that maintains homeostasis in the human body cannot be observed directly. Instead, it is measured through somatic genetic or epigenetic mutations in tissues. Mathematical analysis of somatic clone sizes provides quantitative information on cell birth, death, and replacement rates. This review explores the use of different somatic mutation types for lineage tracing in human tissues, introduces mathematical concepts for inferring dynamic information from clone size data, and discusses the implications of this approach for understanding homeostasis and cancer development.
JOURNAL OF PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Michael Spencer Chapman, Anna Maria Ranzoni, Brynelle Myers, Nicholas Williams, Tim H. H. Coorens, Emily Mitchell, Timothy Butler, Kevin J. Dawson, Yvette Hooks, Luiza Moore, Jyoti Nangalia, Philip S. Robinson, Kenichi Yoshida, Elizabeth Hook, Peter J. Campbell, Ana Cvejic
Summary: By reconstructing a phylogenetic tree of blood development in healthy human fetuses, it was found that individual haematopoietic progenitors acquire multiple somatic mutations by 18 weeks after conception. The data also support a hypoblast origin of the extra-embryonic mesoderm and primitive blood in humans.
Article
Cell & Tissue Engineering
Sierra H. Root, Ivana Vrhovac Madunic, Mark S. Kronenberg, Ye Cao, Sanja Novak, Ivo Kalajzic
Summary: Regulator of G protein signaling 5 (RGS5) acts as a GTPase activator for heterotrimeric G-protein alpha-subunits and is a marker of pericytes. A transgenic mouse model (Rgs5-CreER) was generated for lineage tracing which showed the presence of Rgs5+ cells within endothelial, hematopoietic, and mesenchymal/perivascular cells. Rgs5+ cells expressed osterix and osteocalcin, contributing to new bone formation primarily within the BM niche.
Review
Biochemistry & Molecular Biology
Maria Figueres-Onate, Rebeca Sanchez-Gonzalez, Laura Lopez-Mascaraque
Summary: Understanding the strategies used to study cell lineages in the brain and their role in deciphering functional clonal relationships among neural cells; future perspectives will focus on studying cell heterogeneity and the origins of different cell lineages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jinbang Wang, Tingting Li, Yunting Zhou, Xiaohang Wang, Vladmir Carvalho, Chengming Ni, Wei Li, Qianqian Wang, Yang Chen, Zhanjia Shang, Shanhu Qiu, Zilin Sun
Summary: Stellate cells are crucial for the development of pancreatic fibrosis, both in the exocrine and islet compartments, and their contribution to myofibroblasts has been substantiated.
Article
Biochemistry & Molecular Biology
Dian Yang, Matthew G. Jones, Santiago Naranjo, William M. Rideout III, Kyung Hoi (Joseph) Min, Raymond Ho, Wei Wu, Joseph M. Replogle, Jennifer L. Page, Jeffrey J. Quinn, Felix Horns, Xiaojie Qiu, Michael Z. Chen, William A. Freed-Pastor, Christopher S. McGinnis, David M. Patterson, Zev J. Gartner, Eric D. Chow, Trever G. Bivona, Michelle M. Chan, Nir Yosef, Tyler Jacks, Jonathan S. Weissman
Summary: Tumor evolution is driven by progressive genetic and epigenetic alterations, enabling unrestricted growth and expansion. This study provides insights into the hierarchical nature of tumor evolution through tracking phylogenetic relationships between cancer cells, allowing for in-depth studies of tumor progression.
Review
Immunology
Longyun Ye, Saimeng Shi, Wei Chen
Summary: Pancreatic cancer patients do not benefit from immunotherapy like patients with gastrointestinal tumors, potentially due to the presence of innate immune lymphocytes in the tumor microenvironment. Further research on the function and origin of these lymphocytes could provide guidance for immunotherapy in pancreatic cancer.
FRONTIERS IN IMMUNOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Carmen Gerlach, Scott M. Loughhead, Ulrich H. von Andrian
Article
Immunology
Carmen Gerlach, E. Ashley Moseman, Scott M. Loughhead, David Alvarez, Anthonie J. Zwijnenburg, Lisette Waanders, Rohit Garg, Juan C. de la Torre, Ulrich H. von Andrian
Article
Cell Biology
Harold R. Neely, Irina B. Mazo, Carmen Gerlach, Ulrich H. von Andrian
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2018)
Editorial Material
Immunology
Liv Eidsmo, Carmen Gerlach
TRENDS IN IMMUNOLOGY
(2018)
Editorial Material
Immunology
Carmen Gerlach
TRENDS IN IMMUNOLOGY
(2018)
Article
Hematology
Monika C. Wolkers, Carmen Gerlach, Ramon Arens, Edith M. Janssen, Patrick Fitzgerald, Ton N. Schumacher, Jan Paul Medema, Douglas R. Green, Stephen P. Schoenberger
Article
Multidisciplinary Sciences
Shalin H. Naik, Leila Perie, Erwin Swart, Carmen Gerlach, Nienke van Rooij, Rob J. de Boer, Ton N. Schumacher
Article
Multidisciplinary Sciences
Carmen Gerlach, Jan C. Rohr, Leila Perie, Nienke van Rooij, Jeroen W. J. van Heijst, Arno Velds, Jos Urbanus, Shalin H. Naik, Heinz Jacobs, Joost B. Beltman, Rob J. de Boer, Ton N. M. Schumacher
Review
Immunology
Jan C. Rohr, Carmen Gerlach, Lianne Kok, Ton N. Schumacher
TRENDS IN IMMUNOLOGY
(2014)
Article
Cell Biology
Patricia A. Possik, Judith Mueller, Carmen Gerlach, Juliana C. N. Kenski, Xinyao Huang, Aida Shahrabi, Oscar Krijgsman, Ji-Ying Song, Marjon A. Smit, Bram Gerritsen, Cor Lieftink, Kristel Kemper, Magali Michaut, Roderick L. Beijersbergen, Lodewyk Wessels, Ton N. Schumacher, Daniel S. Peeper
Editorial Material
Immunology
Anthonie J. Zwijnenburg, Carmen Gerlach
Review
Immunology
Shaima Al Khabouri, Carmen Gerlach
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2020)
Editorial Material
Immunology
Klaas P. J. M. van Gisbergen, Carmen Gerlach
Summary: The expansion capacity of naive T cells is believed to be the main factor determining the magnitude of CD8 T-cell responses against intracellular pathogens. However, a study challenges this notion and reveals that the recruitment of naive T-cell clones into primary responses can be incomplete, especially when there are low-affinity interactions between the T-cell receptor and the pathogen's antigen. This research shows that the regulation of CD8 T-cell response size involves control at the level of recruitment and expansion of naive CD8 T cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Correction
Immunology
Carmen Gerlach
TRENDS IN IMMUNOLOGY
(2018)
