期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 66, 期 9, 页码 2649-2658出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erv075
关键词
Carbohydrate active enzymes; cell wall; Golgi apparatus; proteomics; subcellular fraction; wheat grain outer layers
资金
- INRA-CEPIA AIC
Wheat grain outer layers, decisive for development, protection and end-uses, comprise several specialized layers. Cell wall heterogeneity is highlighted and correlated to probable differences in composition of wall machineries.Cereal grain outer layers fulfil essential functions for the developing seed such as supplying energy and providing protection. In the food industry, the grain outer layers called 'the bran' is valuable since it is rich in dietary fibre and other beneficial nutriments. The outer layers comprise several tissues with a high content in cell wall material. The cell wall composition of the grain peripheral tissues was investigated with specific probes at a stage of active cell wall synthesis. Considerable wall diversity between cell types was revealed. To identify the cellular machinery involved in cell wall synthesis, a subcellular proteomic approach was used targeting the Golgi apparatus where most cell wall polysaccharides are synthesized. The tissues were dissected into outer pericarp and intermediate layers where 822 and 1304 proteins were identified respectively. Many carbohydrate-active enzymes were revealed: some in the two peripheral grain fractions, others only in one tissue. Several protein families specific to one fraction and with characterized homologs in other species might be related to the specific detection of a polysaccharide in a particular cell layer. This report provides new information on grain cell walls and its biosynthesis in the valuable outer tissues, which are poorly studied so far. A better understanding of the mechanisms controlling cell wall composition could help to improve several quality traits of cereal products (e.g. dietary fibre content, biomass conversion to biofuel).
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