Article
Biochemistry & Molecular Biology
Shuo Han, Lifu Chen, Yi Zhang, Shihui Xie, Jiali Yang, Songkun Su, Hong Yao, Peiying Shi
Summary: This paper explores the effect of lotus bee pollen extract on cardiomyocyte hypertrophy (CH) and its mechanism. The study found that the extract can reduce oxidative stress levels, inhibit inflammatory response and apoptosis, and protect against CH. Furthermore, it was revealed that the extract targets the JAK2/STAT3 pathway to exert its protective effect on cardiomyocytes.
Article
Biochemistry & Molecular Biology
Jamal Bouitbir, Miljenko V. Panajatovic, Stephan Kraehenbuehl
Summary: This study investigated the effects of imatinib and sorafenib on cardiac toxicity, and found that this cardiac toxicity may be associated with mitochondrial toxicity, through impairing the function of enzyme complexes of the electron transfer system (ETS), causing mitochondrial oxidative stress, and inducing cell death by apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Lygia Sega Nogueira, Carolina P. Vasconcelos, Jessica Rodrigues Placa, Geovanni Pereira Mitre, Leonardo Oliveira Bittencourt, Maria Sueli da Silva Kataoka, Edivaldo H. C. de Oliveira, Rafael Rodrigues Lima
Summary: The study aims to investigate the gene expression changes in human salivary gland cells after exposure to MeHg through transcriptome analysis. The results showed that MeHg altered the expression of 155 genes, with upregulated genes mainly related to cell-cycle progression, DNA repair, and GSH basal metabolism, while downregulated genes were associated with impaired cell metabolism.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Saifudeen Ismael, R. Renuka Nair
Summary: Metabolic shift from fatty acid to glucose is detrimental for cardiovascular health, while reactivation of fatty acid metabolism with medium chain triglycerides shows beneficial effects in reducing cardiomyocyte hypertrophy. The protective effect of medium chain fatty acid is possibly mediated by stimulation of fatty acid metabolism and modulation of signaling pathways over expressed in cardiac hypertrophy.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Pamela Santos-Aguilar, Judith Bernal-Ramirez, Eduardo Vazquez-Garza, Lydia Yerid Velez-Escamilla, Omar Lozano, Gerardo de Jesus Garcia-Rivas, Flavio F. Contreras-Torres
Summary: The study evaluated the cytotoxicity of titanium dioxide (TiO2) particles in cardiovascular system cells. Both TiO2-anatase and TiO2-rutile particles showed similar cytotoxic effects at lower concentrations, but TiO2-rutile particles exhibited greater cytotoxicity at higher concentrations.
Article
Biochemistry & Molecular Biology
Vanessa Naffaa, Isabelle Hochar, Cheryane Lama, Romain Magny, Anne Regazzetti, Pierre Gressens, Olivier Laprevote, Nicolas Auzeil, Anne-Laure Schang
Summary: This study evaluated the effect of bisphenol A (BPA) on oligodendrocyte differentiation by analyzing the cell lipidome. The results suggest that BPA disrupts lipid remodeling during early oligodendrocyte differentiation.
Article
Environmental Sciences
Abdulrahman AL-Ammari, Lei Zhang, Jiazhi Yang, Feng Wei, Chuntao Chen, Dongping Sun
Summary: This study evaluates the toxicity of titanium dioxide nanoparticles on freshwater algae and zebrafish liver cells. Different types of TiO2 NPs showed varying toxic effects on the cells, with short-term exposure affecting cell growth and long-term exposure leading to more significant toxicity. Cell growth was influenced by the dosage, physical properties, and interactions of TiO2 particles with the cells, highlighting the importance of particle size and dispersibility in cytotoxicity. Despite overall toxicity towards algae, the photocatalytical properties of TiO2 NPs may enhance photosynthesis at low concentrations.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Justin Scott, Ryan Grewe, Matteo Minghetti
Summary: This study compared the sensitivity of the Fathead minnow fish embryo acute toxicity (FET) test, the fish gill cells (RTgill-W1) in vitro assay, and the fish larvae acute toxicity test for evaluating whole-effluent toxicity (WET) testing. The results showed a significant correlation between FET and fish larvae tests, while no correlation was found between RTgill-W1 cells and FET. The in vitro alternative models demonstrated good predictability of toxicity in fish for WET chemicals.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hans K. Ghayee, Yiling Xu, Heather Hatch, Richard Brockway, Asha S. Multani, Tongjun Gu, Wendy B. Bollag, Adina Turcu, William E. Rainey, Juilee Rege, Kazutaka Nanba, Vikash J. Bhagwandin, Fiemu Nwariaku, Victor Stastny, Adi F. Gazdar, Jerry W. Shay, Richard J. Auchus, Sergei G. Tevosian
Summary: The human adrenal cortex consists of distinct zones and is the main source of steroid hormone production. Understanding the mechanism of adrenocortical cell differentiation has been challenging, but a patient-derived HAA1 cell line has been discovered that shows unique gene expression patterns upon treatment with histone deacetylase inhibitors. This novel cell line could provide valuable insights into adrenocortical differentiation and steroidogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Lorena Lopez-Suarez, Sana Al Awabdh, Xavier Coumoul, Caroline Chauvet
Summary: Investigating the toxicity of chemicals on the human brain traditionally relies on animal and cell models, which have limitations due to species differences, ethical concerns, and time/cost constraints. Human cell models, such as the SH-SY5Y cell line, offer a relevant alternative for studying neurotoxicity and prioritizing in vivo testing. This review examines the characteristics and use of the SH-SY5Y cell line for studying the effects of organic environmental pollutants on neurotoxicity, highlighting the impacts on cellular processes and neurotransmitter homeostasis.
Article
Cell Biology
Iraselia Garcia, Fredy Calderon, Patrick De la Torre, Shaynah St Vallier, Cristobal Rodriguez, Divya Agarwala, Megan Keniry, Wendy Innis-Whitehouse, Robert Gilkerson
Summary: This study found that in cardiomyoblasts, differentiation to a cardiac-like phenotype via treatment with retinoic acid results in robust cleavage and degradation of long OPA1 isoforms (L-OPA1) under loss of mitochondrial transmembrane potential, suggesting a novel developmental regulatory mechanism for OPA1 cleavage.
Article
Multidisciplinary Sciences
Jing Pan, Qianhui Ren, Zhao Yang, Ying Guo, Kubin Kwon, Checheng Shen, Yueying Wang, Fang Ji
Summary: Melatonin plays a regulatory role in the cell number, differentiation, and mineralization of ALCs. It limits the cell number of ALCs and promotes the production of actin fibers and mRNA levels of enamel matrix proteins. Analysis of RNA-seq data suggests the involvement of Wnt signaling pathway in the differentiation of ALCs under the influence of melatonin.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Michael K. Saad, John S. K. Yuen Jr, Connor M. Joyce, Xinxin Li, Taehwan Lim, Talia L. Wolfson, Justin Wu, Jason Laird, Sanjana Vissapragada, Olivia P. Calkins, Adham Ali, David L. Kaplan
Summary: Cell-cultivated fish has the potential to improve the ethics, sustainability, and safety of the seafood system. However, research on fish cell culture is limited compared to mammalian cells. This study established and characterized a continuous Atlantic mackerel skeletal muscle cell line (Mack cells). The cells were isolated from fresh-caught fish and demonstrated muscle and adipocyte-like phenotypes, providing a valuable reference for future research.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Melanie K. Stewart, Pascal Bernard, Ching-Seng Ang, Deidre M. Mattiske, Andrew J. Pask
Summary: This study investigated the mechanism of estrogen impact on gonad differentiation and ovarian development, revealing that estrogen promotes the shift in gonadal somatic cell fate by activating the MAP3K1 pathway. The activation of MAP3K1 leads to the transition from SOX9 pro-testis activity to beta-catenin pro-ovary activity. These findings provide new insights into the effects of exogenous estrogen exposure on the testis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Moumita Chakrabarti, Ganesh Kumar Raut, Nishant Jain, Manika Pal Bhadra
Summary: The research found that overexpression of PHB1 can alleviate and restore the oxidative stress and mitochondrial morphology changes induced by cardiac hypertrophy. Further experimental results showed that overexpressing PHB1 can maintain mitochondrial quality through blocking pro-hypertrophic pathway, restoring mitochondrial membrane polarization, promoting mitochondrial biogenesis, improving mitochondrial respiratory capacity, and triggering mitophagy.
BIOLOGY OF THE CELL
(2023)
Article
Biochemistry & Molecular Biology
Rui F. Simoes, Paulo J. Oliveira, Teresa Cunha-Oliveira, Francisco B. Pereira
Summary: The prevalence of neurodegenerative diseases is increasing with aging population, but diagnostics and therapeutics have not seen significant improvements. Studying and modulating mitochondrial alterations offer promising strategies for predicting and preventing neurotoxicity and neuronal cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Kobi Wasner, Semra Smajic, Jenny Ghelfi, Sylvie Delcambre, Cesar A. Prada-Medina, Evelyn Knappe, Giuseppe Arena, Patrycja Mulica, Gideon Agyeah, Aleksandar Rakovic, Ibrahim Boussaad, Katja Badanjak, Jochen Ohnmacht, Jean-Jacques Gerardy, Masashi Takanashi, Joanne Trinh, Michel Mittelbronn, Nobutaka Hattori, Christine Klein, Paul Antony, Philip Seibler, Malte Spielmann, Sandro L. Pereira, Anne Gruenewald
Summary: “Parkin plays a crucial role in regulating mitophagy, mitochondrial biogenesis, and mtDNA maintenance, protecting midbrain neurons from neuroinflammation and degeneration.”
MOVEMENT DISORDERS
(2022)
Article
Agriculture, Dairy & Animal Science
Joao Campos Santos, Carla Cruz Marques, Maria Conceicao Baptista, Jorge Pimenta, Jose Teixeira, Liliana Montezinho, Fernando Cagide, Fernanda Borges, Paulo J. Oliveira, Rosa M. L. N. Pereira
Summary: The study investigates the effect of a mitochondria-targeted dietary antioxidant on bovine sperm function. The results show that this antioxidant improves sperm mitochondrial function, fertilization process, and embryo production, suggesting its potential therapeutic strategy to improve the outcomes of assisted reproductive techniques.
Article
Chemistry, Medicinal
Sofia Benfeito, Carlos Fernandes, Daniel Chavarria, Sandra Barreiro, Fernando Cagide, Lisa Sequeira, Jose Teixeira, Renata Silva, Fernando Remiao, Paulo J. Oliveira, Eugenio Uriarte, Fernanda Borges
Summary: Although TPP+ cation is commonly used to target antioxidants to mitochondria, it has shown cytotoxicity in vitro. This study explored nitrogen-derived cationic carriers as alternative compounds to TPP. The results showed that these nitrogen-based cationic carriers were not cytotoxic and exhibited neuroprotective effects against oxidative insults.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Environmental Sciences
Jelena Stevanovic-Silva, Jorge Beleza, Pedro Coxito, Paulo J. Oliveira, Antonio Ascensao, Jose Magalhaes
Summary: Maternal high-caloric nutrition and gestational diabetes mellitus (GDM) increase the risk of liver metabolic alterations in mothers and offspring. Exercise is recommended in GDM treatment. This study showed that gestational exercise protected mothers from diet-induced GDM metabolic consequences and ameliorated liver mitochondrial alterations in offspring.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Ines Ramos Rego, Daniela Silverio, Maria Isabel Eufrasio, Sandra Sofia Pinhancos, Bruna Lopes da Costa, Jose Teixeira, Hugo Fernandes, Yang Kong, Yao Li, Stephen H. Tsang, Paulo J. Oliveira, Rosa Fernandes, Peter M. J. Quinn, Paulo Fernando Santos, Antonio Francisco Ambrosio, Celso Henrique Alves
Summary: Age-related macular degeneration (AMD) is a major cause of visual impairment and blindness in the elderly. The damage to retinal pigment epithelium (RPE) caused by oxidative stress is a key factor in the development of AMD, and this damage is caused by the accumulation of reactive oxygen species (ROS) primarily produced by mitochondria. The protein TRAP1, found in mitochondria, helps maintain mitochondrial integrity and reduces ROS production. This study found that TRAP1 is present in human RPE cells and its levels decrease when exposed to hydrogen peroxide. Silencing TRAP1 leads to increased ROS production and decreased mitochondrial respiratory capacity in RPE cells.
Article
Biochemistry & Molecular Biology
Claudia Bessa, Joana B. Loureiro, Matilde Barros, Vera M. S. Isca, Vilma A. Sardao, Paulo J. Oliveira, Raquel L. Bernardino, Carina Herman-de-Sousa, Maria Adelina Costa, Paulo Correia-de-Sa, Marco G. Alves, Patricia Rijo, Lucilia Saraiva
Summary: This study identified a compound called Roy-Bz, which activates PKC delta and inhibits both mitochondrial respiration and glycolysis in colon cancer cells. These findings suggest a potential dual role of PKC delta in tumor cell metabolism and highlight the therapeutic potential of Roy-Bz in targeting glucose metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Jose P. Castelao-Baptista, Sara A. Valente, Sara Canario, David Oppolzer, Ana Barros, Carlos Venancio, Tania Martins, Luis Antunes, Vilma A. Sardao, Eduardo Rosa, Paulo J. Oliveira
Summary: Obesity, a rising concern in the Eastern world, is often accompanied by non-alcoholic fatty liver disease (NAFLD). Researchers are investigating potential natural interventions to reduce obesity complications. This study focuses on Brassica by-products (BBPs) as an example of edible plant waste and examines the effects of different extracts from broccoli by-products on an in vitro model of fatty acid-induced liver toxicity. The extracts showed a dose-dependent decrease in cell viability of HepG2 cells, but did not compromise mitochondrial function or lipid accumulation. They effectively reduced lipid accumulation induced by fatty acids in HepG2 cells. The extracts' ability to prevent decrease in catalase activity may explain the observed effects.
Article
Nutrition & Dietetics
Diana Sousa, Mariana Rocha, Andreia Amaro, Marcos Divino Ferreira-Junior, Keilah Valeria Naves Cavalcante, Tamaeh Monteiro-Alfredo, Catia Barra, Daniela Rosendo-Silva, Lucas Paulo Jacinto Saavedra, Jose Magalhaes, Armando Caseiro, Paulo Cezar de Freitas Mathias, Susana P. Pereira, Paulo J. Oliveira, Rodrigo Mello Gomes, Paulo Matafome
Summary: Obesogenic environments during the perinatal period can reprogram offspring energy balance mechanisms and increase the risk of metabolic diseases in adulthood. Different obesogenic models have varying effects on metabolic parameters and energy storage pathways in offspring.
Review
Chemistry, Medicinal
Mario P. Marques, Laura Mendonca, Beatriz G. Neves, Carla Varela, Paulo Oliveira, Celia Cabral
Summary: This review investigates the traditional applications of Lamiaceae medicinal plants used by local communities in the Iberian Peninsula for wound healing. The information from Iberian ethnobotanical surveys is summarized, and the pharmacological evidence for eight out of twenty-nine Lamiaceae species is presented. Future studies should focus on isolating and identifying the active molecules of these plants, followed by robust clinical trials to confirm their safety and effectiveness in wound healing.
Article
Biochemical Research Methods
Patrycja Mulica, Carmen Venegas, Zied Landoulsi, Katja Badanjak, Sylvie Delcambre, Maria Tziortziou, Soraya Hezzaz, Jenny Ghelfi, Semra Smajic, Jens Schwamborn, Rejko Krueger, Paul Antony, Patrick May, Enrico Glaab, Anne Gruenewald, Sandro L. Pereira
Summary: This study compares two methods for generating iPSC-derived astrocytes and finds significant differences in the properties of the resulting cells. While one method requires a longer time and higher complexity, the astrocytes generated are more mature.
BIOLOGICAL PROCEDURES ONLINE
(2023)
Article
Medicine, Research & Experimental
Luis F. Grilo, Joao D. Martins, Mariana S. Diniz, Carolina Tocantins, Chiara H. Cavallaro, Ines Baldeiras, Teresa Cunha-Oliveira, Stephen Ford, Peter W. Nathanielsz, Paulo J. Oliveira, Susana P. Pereira
Summary: Maternal obesity may significantly affect liver function and pathophysiology, increasing the risk of pregnancy-associated liver diseases and hepatic diseases. Oxidative stress and mitochondrial dysfunction may play crucial roles in this process.
Article
Environmental Sciences
Elsa T. Rodrigues, Eduarda Pereira, Paulo J. Oliveira, Miguel A. Pardal
Summary: The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. It also highlights the capability of this assay to discriminate influent and effluent toxic characteristics and its suitability for water quality monitoring and surveillance of treatment processes.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Medicine, General & Internal
Aryane Cruz Oliveira Pinho, Diana Santos, Paulo J. Oliveira, Ermelindo Carreira Leal, Eugenia Carvalho
Summary: This study investigated the effects of diabetes on mitochondrial respiration in wounded and non-wounded skin using high-resolution respirometry. The results showed that the oxidative phosphorylation capacity of the wounded skin was decreased at day 3 post-wounding and increased at day 10 post-wounding in diabetic mice. The relative contribution of pyruvate, malate, and glutamate oxidation to the oxidative phosphorylation capacity was higher in wounded skin, while fatty acid oxidation contributed more to the non-wounded skin. This study provides important insights into the impact of diabetes on wound healing.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)