Article
Biochemistry & Molecular Biology
Sara McCurdy, Jenny Lin, Robert Shenkar, Thomas Moore, Rhonda Lightle, Eva Faurobert, Miguel-Alejandro Lopez-Ramirez, Issam Awad, Mark H. Ginsberg
Summary: Beta 1 integrins play a crucial role in blood vessel formation and function, and their loss can lead to brain microvascular defects. Targeting beta 1 integrin with an activated-specific antibody can reduce the development of CCM lesions.
Article
Cell Biology
Jesus Vega-Lugo, Bruno da Rocha-Azevedo, Aparajita Dasgupta, Khuloud Jaqaman
Summary: The conditional colocalization analysis method can be used to study the colocalization relationships between three molecular entities in three-color microscopy images, going beyond traditional analyses designed for two-color images. This approach helps to address the influence of colocalization between molecules and showcases its application in functionally relevant plasma membrane locations.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Ke Zhu, Kamalika Mukherjee, Changli Wei, Salim S. Hayek, Agnieszka Collins, Changkyu Gu, Kristin Corapi, Mehmet M. Altintas, Yong Wang, Sushrut S. Waikar, Antonio C. Bianco, Alexander Koch, Frank Tacke, Jochen Reiser, Sanja Sever
Summary: D2D3 protein is associated with kidney diseases and insulin-dependent diabetes mellitus. It impairs the function of pancreatic beta cells and the kidneys, and targeting this protein could potentially provide a therapy.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Michael Warkala, Dongying Chen, AnnJosette Ramirez, Ali Jubran, Michael Schonning, Xia Wang, Huaning Zhao, Sophie Astrof
Summary: The development of the fourth pharyngeal arch arteries involves a multistep process with contributions from SHF-derived ECs, remodeling of endothelial plexus into PAAs, and remodeling of PAAs into the aortic arch artery and its major branches. Cell-ECM interactions regulated by integrin alpha 5 beta 1 and Fn1 are essential at each developmental stage.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Naseeb K. Malhi, Claire L. Allen, Elizabeth Stewart, Katherine L. Horton, Federica Riu, Jennifer Batson, Winfried Amoaku, Jonathan C. Morris, Kenton P. Arkill, David O. Bates
Summary: This study demonstrates that SRPK1 is activated in diabetes, and its inhibition can switch VEGF splicing, reducing retinal permeability and edema in diabetic retinopathy.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vincenza Cifarelli, Vivek S. Peche, Nada A. Abumrad
Summary: The vascular and lymphatic systems in the gut play important roles in regulating lipid transport, immune cell trafficking, and preventing the transfer of commensal gut microbiota into the bloodstream. Dysfunctions in these systems can lead to tissue inflammation and systemic metabolic abnormalities. Lipid metabolism is crucial for maintaining the integrity and function of intestinal endothelial cells. This review focuses on recent progress in understanding the role of lipid transporters, particularly CD36, in maintaining gastrointestinal barrier integrity, inflammation, and overall whole body metabolism. The potential impact of endothelial lipid delivery on tissue renewal and the development of gastric and intestinal diseases is also discussed.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Virology
Stefan Hagele, Christian Nusshag, Alexander Muller, Alexandra Baumann, Martin Zeier, Ellen Krautkramer
Summary: The human respiratory epithelium is one of the initial targets of orthohantaviral infection, contributing to virus replication, dissemination, and pathogenesis of HFRS-causing orthohantaviruses. Donor-specific factors may lead to differences in the severity and symptoms of orthohantavirus disease, indicating an alternative mode of orthohantaviral entry independent of integrin beta(3).
Article
Toxicology
Wei Chen, Haotian Yu, Chengbiao Sun, Mingxin Dong, Na Zhao, Yan Wang, Kaikai Yu, Jianxu Zhang, Na Xu, Wensen Liu
Summary: This study reveals that ?-bungarotoxin (?-BGT) from snake venom can interfere with the vascular endothelial homeostasis by directly binding to cell surface integrins, leading to barrier dysfunction. ?-BGT selectively binds to integrin α5 in vascular endothelium, initiating downstream events including focal adhesion kinase dephosphorylation and cytoskeleton remodeling, which disrupt intercellular junctions and increase paracellular permeability. Cyclin D1 mediates the cellular structural changes and barrier dysfunction, while plasminogen activator urokinase and platelet-derived growth factor D could serve as potential diagnostic biomarkers for ?-BGT-induced vascular endothelial dysfunction.
TOXICOLOGY LETTERS
(2023)
Article
Biochemical Research Methods
Jaehoon Kim, Hyunho Kim, Jeong Suk Kang, Eun Soo Lee, Choon Hee Chung, Hyun Jeong Oh, YongTae Kim, Seok Chung, Eun Young Lee
Summary: We have developed a microfluidic model that replicates the physiology of the glomerular filtration barrier (GFB) through tunable glomerular basement membrane (gBM) deposition and 3D co-culture of podocytes with glomerular endothelial cells (gECs). By controlling the thickness of the gBM, our model successfully reproduces the biphasic response of the GFB and facilitates dynamic crosstalk between gECs and podocytes. The interaction of gECs and podocytes plays a significant role in drug-induced injury and regulation of barrier properties.
Article
Engineering, Biomedical
Bo Zhang, Yumei Qin, Li Yang, Huining Wan, Lu Yuan, Yunbing Wang
Summary: This study developed a new stent that combines the biological functions of nitric oxide gas and VEGF to promote the proliferation and migration of endothelial cells while suppressing smooth muscle cells. The functionalized stent showed enhanced vascular healing and reduced inflammation and vessel wall injury.
Article
Biochemistry & Molecular Biology
Esmaeil Samadian, Abasalt Hosseinzadeh Colagar, Mahdieh Safarzad, Jahanbakhsh Asadi, Kamran Mansouri
Summary: The study investigated the impact of Urtica dioica agglutinin (UDA) on cellular vascularization processes. UDA inhibited cancer cell growth and vascularization while showing minimal toxicity to normal cells. UDA binds to glyco-conjugates containing GlcNAc/man oligomers to exert its anti-angiogenic activity, suggesting its therapeutic importance in angiogenesis-related treatments.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Yosuke Kanno
Summary: Fibrotic diseases are characterized by excessive extracellular matrix deposition leading to tissue overgrowth. The progression of fibrosis is driven by increased ECM production and impaired degradation, which can result from inflammation, vascular dysfunction, and immune abnormalities. Recent studies have shown that the urokinase plasminogen activator (uPA) and its receptor (uPAR) play a role in regulating the inflammatory response, vascular homeostasis, and immune homeostasis, making them potential therapeutic targets for fibrotic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maxim M. M. Zaigraev, Ekaterina N. N. Lyukmanova, Alexander S. S. Paramonov, Zakhar O. O. Shenkarev, Anton O. O. Chugunov
Summary: Ly6/uPAR proteins with GPI-anchors have various positions and orientations on the membrane surface, regardless of the linker length between the LU domain and GPI-anchor. The proteins interact with the membrane through polypeptide parts and glycans. Different proteins have different contact regions with the membrane and unique orientations, indicating that GPI-anchoring does not determine the optimal pre-orientation for receptor interaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Xueqing Tang, Jingwei Bian, Zijian Li
Summary: GPCR internalization can trigger signal activation, providing new insights and drug targets for receptor internalization. The process is tightly regulated, particularly through posttranslational modifications. This review summarizes different posttranslational modifications in GPCR internalization, analyzing their significance in internalization dynamics, routes, fates, and related diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Medicine, General & Internal
Filomena Napolitano, Nunzia Montuori
Summary: The plasminogen activation system plays a crucial role not only in regulating fibrinolysis, but also in various biological processes. The link between this system and angioedema has become a topic of scientific debate, with an increasing number of studies investigating its involvement in recent years.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Kumaresan Jayaraman, Anand K. Das, Dino Luethi, Daniel Szoellosi, Gerhard J. Schuetz, Maarten E. A. Reith, Harald H. Sitte, Thomas Stockner
Summary: Transporters of the SLC6 family mediate neurotransmitter reuptake, forming various quaternary structures that support the activity of release-type psychostimulants. Single molecule microscopy experiments have revealed differences in stoichiometry between individual members.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Oncology
Matthias Unseld, Jelena Belic, Kerstin Pierer, Qing Zhou, Tina Moser, Raimund Bauer, Gudrun Piringer, Armin Gerger, Alexander Siebenhuener, Michael Speicher, Ellen Heitzer, Gerald W. Prager
Summary: This study found that higher levels of ctDNA are associated with survival in metastatic colorectal cancer patients treated with regorafenib. The benefit of combining evaluation of mutations and somatic copy number alterations was highlighted in advanced cancer patients.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Oncology
Gerald W. Prager, Leopold Oehler, Armin Gerger, Brigitte Mlineritsch, Johannes Andel, Andreas Petzer, Klaus Wilthoner, Thamer Sliwa, Petra Pichler, Thomas Winder, Sonja Heibl, Birgit Gruenberger, Friedrich Laengle, Eva Hubmann, Markus Korger, Martin Pecherstorfer, Angela Djanani, Hans-Joerg Neumann, Kathrin Philipp-Abbrederis, Ewald Woll, Robert Trondl, Catharina Arnold-Schrauf, Wolfgang Eisterer
Summary: This study confirms the feasibility and tolerability of nab-P/G treatment in younger and elderly patients with mPC, with similar efficacy. Older patients were more likely to experience fatigue and reduced appetite, but overall response rates, progression-free survival, and overall survival were comparable between younger and elderly patients.
EUROPEAN JOURNAL OF CANCER
(2021)
Meeting Abstract
Oncology
Thomas Seufferlein, Thomas Jens Ettrich, Alexander Stein, Dirk Arnold, Gerald W. Prager, Stefan Kasper, Michael Niedermeier, Lothar Muller, Stefan Kubicka, Alexander Koenig, Petra Buchner-Steudel, Kai Wille, Angelika M. R. Kestler, Andreas W. Berger, Lukas Perkhofer, Ludwig Lausser, Hans A. Kestler
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Meeting Abstract
Oncology
Julien Taieb, Pascal Hammel, Michele Reni, Daniel H. Palmer, John A. Bridgewater, Antonio Cubillo, Gerald W. Prager, Alice Vermeire, Nathalie D'Esquermes, Fabienne Biville-Hedouin, Teng Zhaoyang, Thomas Seufferlein, Teresa Macarulla
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Magdalena C. Schneider, Roger Telschow, Gwenael Mercier, Montserrat Lopez-Martinez, Otmar Scherzer, Gerhard J. Schuetz
Summary: Single molecule localization microscopy (SMLM) has the potential to resolve subcellular structures below the diffraction limit, with high localization precision. Sample fixation is necessary to prevent molecular motion during recording, but can affect the sample's ultrastructure. Performing SMLM at low temperatures can improve localization precision and enable reliable assignment of dye molecules.
Article
Medicine, General & Internal
Hossein Taghizadeh, Leonhard Mullauer, Robert M. Mader, Thorsten Fureder, Gerald W. Prager
Summary: Molecular profiling was used to offer molecular-guided therapies to patients with therapy-refractory metastatic parotid gland malignancies. Genetic aberrations were identified in five patients, leading to targeted therapy recommendations for eight patients, with one patient achieving stable disease for 14 months. This approach may provide feasible treatment options for this rare disease entity.
WIENER KLINISCHE WOCHENSCHRIFT
(2021)
Article
Multidisciplinary Sciences
Fabian Hinterer, Magdalena C. Schneider, Simon Hubmer, Montserrat Lopez-Martinez, Philipp Zelger, Alexander Jesacher, Ronny Ramlau, Gerhard J. Schuetz
Summary: Single molecule localization microscopy (SMLM) has the potential to resolve structural details of biological samples at the nanometer scale. By performing SMLM under cryogenic temperature, higher localization precision can be achieved. However, it is important to consider the anisotropic emission characteristics of dipole emitters with fixed orientation to fully exploit the resolution. This study demonstrates the use of astigmatic imaging and dipole orientation information to extract the position of dipole emitters without bias and reach a precision of 1 nm.
Article
Cell Biology
Tamara Stojanovic, David Velarde Gamez, Gabor Jorrid Schuld, Daniel Bormann, Maureen Cabatic, Pavel Uhrin, Gert Lubec, Francisco J. Monje
Summary: Nicotine addiction mainly develops during adolescence, with miRNA-132/212 playing a role in regulating the effects of nicotine on synaptic functions in the hippocampus of adolescent and adult mice.
Article
Biochemical Research Methods
Johanna Irrgeher, Thomas Berger, Anastassiya Tchaikovsky, Cornelius Tschegg, Ghazaleh Gouya, Peter Lechner, Anika Retzmann, Christine Opper, Christa Firbas, Michael Freissmuth, Kerstin Peschel-Credner, Karolina Anderle, Claudia Meisslitzer, Michael Wolzt, Thomas Prohaska
Summary: This article presents an analysis method for Pb isotope pattern deconvolution using enriched Pb-204 as a tracer to monitor trace levels of Pb within the human body. The method overcomes the limitations of using natural lead as a tracer and allows for the calculation of isotope pattern from measured isotope ratios without knowing the quantities of different isotope sources.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Lenka Molcanova, Jakub Treml, Veronika Brezani, Petr Marsik, Sebnem Kurhan, Zdenek Travnicek, Pavel Uhrin, Karel Smejkal
Summary: Paulownia tomentosa Steud., a traditional Chinese medicinal plant, is rich in phenolic compounds and has potential anti-inflammatory activities. This study isolated several flavonoids from the fruit of P. tomentosa and evaluated their cytotoxicity and anti-inflammatory effects. The results showed that these flavonoids reduced the activation of NF-KB/AP-1, indicating their potential as a source for new anti-inflammatory medications.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ingrid Hartl, Veronika Brumovska, Yasmin Striedner, Atena Yasari, Gerhard J. Schuetz, Eva Sevcsik, Irene Tiemann-Boege
Summary: This study utilized micropatterning to enrich FGFR3 in specific areas of the cell membrane, and quantified receptor activation by observing the recruitment of downstream signaling molecule GRB2 to FGFR3 micropatterns. The results showed that mutations in FGFR3 altered receptor activity, with increased activity in G380R and K650Q mutants upon ligand addition, while decreased activity in K650E mutant. These findings provide further insight into the functional effects of mutations to FGFR3.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Thomas J. F. Angenoorth, Julian Maier, Stevan Stankovic, Shreyas Bhat, Sonja Sucic, Michael Freissmuth, Harald H. Sitte, Jae-Won Yang
Summary: Organic cation transporters (OCTs) are membrane proteins that uptake monoamines, cationic drugs and xenobiotics. In this study, the researchers investigated two potentially misfolded variants of organic cation transporter 3 (OCT3) and found that pre-treatment with the chemical chaperone 4-PBA led to increased membrane expression and transport capacity of the misfolded variants. This study provides proof of principle that folding-deficient SLC22 transporter variants, especially OCT3, can be rescued by chaperones.
Article
Biochemistry & Molecular Biology
Michael Freissmuth
Summary: The level of dopamine transporters in the neuronal plasma membrane affects learning and motor coordination in mice. Kearney and colleagues recently showed that dopamine autoreceptors and metabotropic glutamate receptors control the cycling of dopamine transporters in the presynaptic specialization of dopaminergic neurons. These findings have important implications for future studies in neural development and motor learning.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Physics, Applied
Fabian Hinterer, Magdalena C. Schneider, Simon Hubmer, Montserrat Lopez-Martinez, Ronny Ramlau, Gerhard J. Schuetz
Summary: Single molecule localization microscopy relies on precise quantification of the positions of single dye emitters in a sample. The number of detected photons from each molecule determines the precision, which can be improved by recording at cryogenic temperatures to reduce photobleaching. However, long illuminations can cause image blur due to inevitable jitter or drift, which degrades localization precision. This paper demonstrates a method to largely eliminate drift effects by parallel recording fiducial marker beads and fitting the drift trajectory, applicable to drift magnitudes of several hundred nanometers per frame, and assuming fixed dipole orientations during each illumination.
APPLIED PHYSICS LETTERS
(2023)