Article
Cell Biology
Suneel Rallapalli, Soma Guhathakurta, Purna S. Korrapati
Summary: The discovery of CPCs has raised hopes for heart cell therapy, but there are still issues with defining CPC types and validating methods for generating high-quality CPC populations. Long-term culture may alter CPC immunophenotypes, highlighting the need for validated molecular signatures to assess the impact of ex vivo expansion on cell therapy products.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fareheh Firouzi, Oscar Echeagaray, Carolina Esquer, Natalie A. Gude, Mark A. Sussman
Summary: The heterogeneity and plasticity of cardiac fibroblast population pose challenges for the study of cardiac biology and intervention strategies. This study evaluates the role of c-Kit biology within the population of cardiac fibroblasts, providing a fundamental basis for future research.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Apoorva S. S. Metkari, Eric W. W. Fowler, Robert L. L. Witt, Xinqiao Jia
Summary: Synthetic matrices with varying degrees of degradability were synthesized to engineer implantable salivary gland constructs. The degradable matrices promoted cell proliferation and development of multicellular structures with a secretary phenotype, as evidenced by the upregulation of acinar markers. Cell-mediated matrix remodeling was found to be necessary for the development of regenerative pro-acinar progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Sheng Xing, Jin-Ze Tian, Shu-Hua Yang, Xue-Ting Huang, Yan-Fu Ding, Qian-Yun Lu, Jin-Shu Yang, Wei-Jun Yang
Summary: Blood vessels in adult mammals are highly organized and stable, but in cases of ischemic heart, c-Kit(+) cells play a crucial role in the development and homeostasis of cardiac endothelial cells. Setd4-expressing quiescent c-Kit(+) cells can regulate vascular endothelial cells and improve cardiac function in myocardial infarction injured mice. This suggests the potential for targeted treatments for cardiac ischemic injury by controlling the quiescence of c-Kit(+) cells through epigenetic mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Cell & Tissue Engineering
Attaur Rahman, Yuhao Li, Nur Izzah Ismail, To-Kiu Chan, Yuzhen Li, Dachun Xu, Hao Zhou, Sang-Bing Ong
Summary: This study investigated the relationship between mitochondrial morphology and the differentiation status of c-kit(+) progenitor cells. Pharmacologically inhibiting mitochondrial fragmentation retains the undifferentiated state of the c-kit(+) progenitor cells, providing a new perspective on directing progenitor cell fate through mitochondrial manipulation.
INTERNATIONAL JOURNAL OF STEM CELLS
(2023)
Article
Biochemistry & Molecular Biology
Meng-Xi Wang, Li Yan, Juan Chen, Jun-Mei Zhao, Jiang Zhu, Shan-He Yu
Summary: This study explored the feasibility of reducing the malignant characteristics of t(8;21) AML cells by reinforcing their endogenous differentiation potential. The results showed that in an AE9a murine model, the process of erythroid differentiation was accompanied by a decline or loss of leukemia-initiating potential. Therefore, enhancing the erythroid differentiation of t(8;21) AML cells may be a promising intervention strategy.
Article
Veterinary Sciences
Hlaing Phyo, Amira Aburza, Katie Mellanby, Cristina L. L. Esteves
Summary: Researchers found that the endometrium of dogs can be used as an alternative source of mesenchymal stem cells (MSCs), which have similar characteristics to MSCs obtained from adipose tissue for therapeutic applications.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Medicine, Research & Experimental
Maria Rosa Iaquinta, Carmen Lanzillotti, Chiara Mazziotta, Ilaria Bononi, Francesca Frontini, Elisa Mazzoni, Lucia Oton-Gonzalez, John Charles Rotondo, Elena Torreggiani, Mauro Tognon, Fernanda Martini
Summary: Mesenchymal stem cells (MSCs) play a crucial role in tissue engineering and regenerative medicine for bone tissue and cartilage. The regulation of miRNAs is important for MSCs differentiation, and dysregulation of miRNAs may be associated with osteoporosis, bone fractures, and bone cancer. Bone-associated circulating miRNAs could be useful biomarkers for diagnosis, follow-up, and treatment of cancer and metastases.
Article
Health Care Sciences & Services
Cristina Blanco-Elices, Jesus Chato-Astrain, Alberto Gonzalez-Gonzalez, David Sanchez-Porras, Victor Carriel, Ricardo Fernandez-Valades, Maria del Carmen Sanchez-Quevedo, Miguel Alaminos, Ingrid Garzon
Summary: This study characterized the human umbilical cord at both in situ and ex vivo levels and revealed the heterogeneity of the cord. Different regions of the cord showed differential expression of extracellular matrix components and vascular markers. These findings have important implications for the selection of cells from specific regions of the umbilical cord for tissue engineering applications.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Cell Biology
Jose Manuel Inacio, Joao von Gilsa Lopes, Ana Mafalda Silva, Fernando Cristo, Sara Marques, Matthias E. Futschik, Jose Antonio Belo
Summary: Deciphering the clues of a regenerative mechanism for the mammalian adult heart, particularly focusing on the role of DAND5, could potentially lead to the development of more effective treatment options for heart failure. Further research on the transcriptional profiling sequencing and quantitative RT-PCR assays of DAND5 may provide insights into the generation and expansion of pluripotent stem cell-derived cardiomyocytes systems for clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
In-Chi Young, Bogang Wu, Jaclyn Andricovich, Sung-Ting Chuang, Rong Li, Alexandros Tzatsos, Ray-Chang Wu, Mei-Yi Wu
Summary: ARID4B interferes with KITLG/KIT signaling, promoting HSC differentiation instead of self-renewal. ARID4B-deficient HSCs self-express KITLG and overexpress KIT, leading to differentiation. Inhibition of Src family kinases rescues the HSC differentiation defect caused by ARID4B loss.
Article
Cell Biology
Ravena P. Nascimento, Balbino L. dos Santos, Karina C. da Silva, Victor D. Amaral da Silva, Maria de Fatima Costa, Jorge M. David, Juceni P. David, Vivaldo Moura-Neto, Mona das N. Oliveira, Henning Ulrich, Giselle P. de Faria Lopes, Silvia L. Costa
Summary: The study found that the flavonoid agathisflavone has a direct anti-glioma effect on human glioblastoma cells, as well as an impact on the response of mesenchymal stem cells, which may influence the control of glioblastoma growth and aggressiveness.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biology
Giacomo Della Verde, Michika Mochizuki, Vera Lorenz, Julien Roux, Lifen Xu, Leandra Ramin-Wright, Otmar Pfister, Gabriela M. Kuster
Summary: Flt3 is a regulator of hematopoietic progenitor cells, and its ligand Flt3L affects cardiac side population progenitor cells (SP-CPCs), leading to increased proliferation and decreased vasculogenic potential in the absence of Flt3L, ultimately resulting in lower cardiac function.
LIFE SCIENCE ALLIANCE
(2021)
Article
Multidisciplinary Sciences
Mikael Sandstedt, Kristina Vukusic, Benjamin Ulfenborg, Marianne Jonsson, Lillemor Mattsson Hulten, Goran Dellgren, Anders Jeppsson, Jane Synnergren, Joakim Sandstedt
Summary: Cardiomyocyte proliferation is the main source of new cardiomyocytes in adults. This study discovered SSEA4+CD34- cells in the adult human heart, which may serve as immature cycling cardiomyocytes and play a role in endogenous cardiac regeneration.
Article
Biochemistry & Molecular Biology
Ilaria Massaiu, Jeness Campodonico, Massimo Mapelli, Elisabetta Salvioni, Vincenza Valerio, Donato Moschetta, Veronika A. Myasoedova, Maria Domenica Cappellini, Giulio Pompilio, Paolo Poggio, Piergiuseppe Agostoni
Summary: By analyzing the gene expression profiles of iron-linked genes at the tissue and single-cell level in heart failure patients, it was found that the iron metabolism in cardiomyocytes is altered, including imbalanced iron ion internalization, decreased internal conversion, increased hemoglobin synthesis, elevated heme externalization, reduced heme cleavage, and up-regulated hepcidin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Medicine, General & Internal
Maurizio Pesce, Giulio Pompilio, Jozef Bartunek
MAYO CLINIC PROCEEDINGS
(2023)
Article
Biochemistry & Molecular Biology
Melania Lippi, Angela Serena Maione, Mattia Chiesa, Gianluca Lorenzo Perrucci, Lara Iengo, Tommaso Sattin, Chiara Cencioni, Matteo Savoia, Andreas M. Zeiher, Fabrizio Tundo, Claudio Tondo, Giulio Pompilio, Elena Sommariva
Summary: Arrhythmogenic cardiomyopathy (ACM) is a genetic disorder characterized by ventricular arrhythmias, contractile dysfunctions and fibro-adipose replacement of myocardium. This study aimed to enhance the understanding of ACM pathogenesis by comparing the epigenetic and gene expression profiles of ACM-CMSCs with healthy control (HC)-CMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Martina Rabino, Erica Rurali, Chiara Zamboni, Davide Rovina, Sara Mallia, Matteo Cauteruccio, Andrea Baggiano, Carlo Maria Giacari, Milena Bellin, Giulio Pompilio
Summary: Coronavirus disease (COVID-19) is caused by SARS-CoV-2 and can lead to respiratory symptoms. Cardiovascular complications are common, including myocarditis, arrhythmias, heart failure, and coagulation abnormalities. In this study, hiPSCs were generated from four COVID-19 patients, two with severe myocarditis and two without, to study the mechanisms underlying the variability in severe cardiac manifestations.
STEM CELL RESEARCH
(2023)
Correction
Physiology
Jose Manuel Pioner, Lorenzo Santini, Chiara Palandri, Marianna Langione, Bruno Grandinetti, Silvia Querceto, Daniele Martella, Costanza Mazzantini, Beatrice Scellini, Lucrezia Giammarino, Flavia Lupi, Francesco Mazzarotto, Aoife Gowran, Davide Rovina, Rosaria Santoro, Giulio Pompilio, Chiara Tesi, Camilla Parmeggiani, Michael Regnier, Elisabetta Cerbai, David L. Mack, Corrado Poggesi, Cecilia Ferrantini, Raffaele Coppini
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Medicine, General & Internal
Reem Saud Alhejailan, Gloria Garoffolo, Vineesh Vimala Raveendran, Maurizio Pesce
Summary: After more than 20 years of research in regenerative medicine, the best cell types and materials to effectively translate into clinical practice for cardiac diseases are still uncertain. It is now clear that the heart lacks a consistent reservoir of stem cells for generating new myocytes, and other cells may only contribute with their pro-angiogenic or immunomodulatory potential. New developments in somatic cells' reprogramming, material science, and cell biophysics may be helpful in protecting the heart and enhancing its lost regenerative potential.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell Biology
Ermes Carulli, Giulio Pompilio, Maria Cristina Vinci
Summary: Type 1 diabetes mellitus (T1DM) is a common autoimmune disease causing the destruction of pancreatic islet beta-cells, resulting in insulin deficiency and reduced life quality. Cell therapy, particularly autologous nonmyeloablative hematopoietic stem cell transplantation (AHST), has shown promising results for restoring insulin independence, but the success rate varies. A comprehensive review of trials involving stem cells, specifically AHST, for T1DM treatment was conducted. Older patients had a significantly higher probability of achieving insulin independence, while patients with a history of ketoacidosis had a significantly lower probability. Further data is needed to determine the ideal candidate profile.
Review
Biochemistry & Molecular Biology
Giovanni Peretto, Elena Sommariva, Chiara Di Resta, Martina Rabino, Andrea Villatore, Davide Lazzeroni, Simone Sala, Giulio Pompilio, Leslie T. Cooper
Summary: In recent years, research has shown that myocardial inflammation (M-Infl) is involved in the pathophysiology and phenotypes of genetically inherited cardiomyopathies, such as dilated and arrhythmogenic cardiomyopathy. M-Infl, which resembles myocarditis, frequently occurs as a clinical manifestation of these cardiac diseases. The emerging role of M-Infl is leading to the discovery of potential targets for treating inflammation and paving the way for new approaches in cardiomyopathies.
Article
Cell & Tissue Engineering
Roman Vuerich, Elena Groppa, Simone Vodret, Nadja Annelies Ruth Ring, Chiara Stocco, Fleur Bossi, Chiara Agostinis, Matteo Cauteruccio, Andrea Colliva, Mohammad Ramadan, Francesca Simoncello, Federica Benvenuti, Anna Agnelli, Franca Dore, Flavia Mazzarol, Massimo Moretti, Alice Paulitti, Silvia Palmisano, Nicolo De Manzini, Mattia Chiesa, Manuel Casaburo, Angela Raucci, Daniela Lorizio, Giulio Pompilio, Roberta Bulla, Giovanni Papa, Serena Zacchigna
Summary: Nonhealing wounds have a negative impact on the quality of life for patients and health systems. Skin substitutes have been used, but have limited efficacy due to inadequate vascularization. The use of the stromal vascular fraction (SVF) from adipose tissue shows promise in overcoming this limitation, but additional research is needed to determine its precise mechanism of action.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biology
Salwa Soussi, Lesia Savchenko, Davide Rovina, Jason S. S. Iacovoni, Andrea Gottinger, Maxime Viallettes, Jose-Manuel Pioner, Andrea Farini, Sara Mallia, Martina Rabino, Giulio Pompilio, Angelo Parini, Olivier Lairez, Aoife Gowran, Nathalie Pizzinat
Summary: A study revealed that DMD patients lack the highest molecular weight isoform of dystrophin in their cardiac fibroblasts, leading to altered cell metabolism and mitochondrial function, exacerbating cardiac fibrosis and deterioration of cardiac function.
Review
Biochemistry & Molecular Biology
Sara Rega, Floriana Farina, Silvia Bouhuis, Silvia de Donato, Mattia Chiesa, Paolo Poggio, Laura Cavallotti, Giorgia Bonalumi, Ilaria Giambuzzi, Giulio Pompilio, Gianluca L. Perrucci
Summary: Thoracic aortic aneurysm (TAA) is a serious condition without etiological therapy. Recent studies have shown that integrating biological targets and etiological mechanisms through -omics approaches, especially metabolomics, can provide valuable insights for TAA diagnosis and therapy.
CELL AND BIOSCIENCE
(2023)
Review
Physiology
Martina Rabino, Elena Sommariva, Serena Zacchigna, Giulio Pompilio
Summary: Genetic cardiomyopathies are inherited disorders that damage the structure and function of myocardium. Personalized models are needed to uncover their pathological mechanisms and develop therapeutic strategies. Human induced pluripotent stem cell-derived endothelial cells have been less studied but represent a suitable model to investigate the role of dysfunctional endothelium in genetic cardiomyopathies. This review summarizes the main studies using these cells to investigate endothelial dysfunction in genetic-based cardiomyopathies and discusses new potential therapeutic targets.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Hematology
Marta Verna, Marta Canesi, Valentino Conter, Lawrence Faulkner, Attilio Maria Rovelli, Daniela Silvestri, Ignazio Majolino, Andrea Biondi, Chra Nawfal Abdullah, Vian Faeq Mohammed
Summary: Thalassemia is a common and life-threatening disorder in children in regions prone to hemoglobinopathy, such as the Middle East. Hematopoietic stem cell transplantation (HSCT) is a highly effective and cost-effective treatment for thalassemia. A study in Iraqi Kurdistan demonstrated the successful establishment of an HSCT unit for both adults and children, achieving comparable results to international standards. The success of the program relied on meeting minimum requirements, international collaboration, and capacity-building efforts.
TRANSPLANTATION AND CELLULAR THERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Shinichiro Masuda, Patrick W. Serruys, Shigetaka Kageyama, Nozomi Kotoku, Kai Ninomiya, Scot Garg, Alan Soo, Marie-Angele Morel, John D. Puskas, Jagat Narula, Ulrich Schneider, Torsten Doenst, Kaoru Tanaka, Johan de Mey, Mark La Meir, Antonio L. Bartorelli, Saima Mushtaq, Giulio Pompilio, Daniele Andreini, Yoshinobu Onuma
Summary: This study compared treatment recommendations based on the SYNTAX score 2020 derived from coronary computed tomography angiography (CCTA) and invasive coronary angiography (ICA). The results showed moderate to substantial agreement between the anatomical SYNTAX scores derived from CCTA and ICA, suggesting that CCTA could be used as an alternative to ICA when making decisions regarding the modality of revascularization.
INTERNATIONAL JOURNAL OF CARDIOVASCULAR IMAGING
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Patrick W. Serruys, Shinichiro Masuda, Shigetaka Kageyama, Nozomi Kotoku, Kai Ninomiya, Ulrich Schneider, Ulf Teichgraber, Torsten Doenst, John D. Puskas, Jagat Narula, Kaoru Tanaka, Johan de May, Mark La Meir, Antonio L. Bartorelli, Saima Mushtaq, Giulio Pompilio, Daniele Andreini, Yoshinobu Onuma
JACC-CARDIOVASCULAR INTERVENTIONS
(2023)