☆ 4.2 Article

Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY (2015)

期刊

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY

卷 298, 期 9, 页码 1644-1658

出版社

WILEY

DOI: 10.1002/ar.23174

关键词

luteinizing hormone; follicle stimulating hormone; somatolactin hormone; prolactin hormone; growth hormone; reproductive dysfunction

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Anatomy & Morphology

资金

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2008/57687-0, 2007/55494-7]
Programa de Mobilidade Internacional de Alunos de Pos-Graduacao-Santander/Banespa
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/57687-0] Funding Source: FAPESP

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摘要

The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), beta-luteinizing hormone (beta-LH), and beta-follicle stimulating hormone (beta-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, beta-LH OD did not vary during the annual reproductive cycle. After AIS, beta-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of beta-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. (C) 2015 Wiley Periodicals, Inc.

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A Quadruplex Ultrasensitive Immunoassay for Simultaneous Assessment of Human Reproductive Hormone Proteins in Multiple Biofluid Samples

Qiyong Cai, Shuiling Jin, Hong Zong, Lu Pei, Ke Cao, Lingbo Qu, Zhaohui Li

Summary: This study developed a quadruplex ultrasensitive immunoassay for simultaneous assessment of four reproductive hormone proteins in various biofluid samples. The assay exhibited good performance and consistency with clinical test results, allowing for rapid and accurate diagnosis of reproductive endocrine disorders.

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