Article
Biochemistry & Molecular Biology
Kuin Tian Pang, Mean Ghim, Padmini Sarathchandra, Christina M. Warboys, Magdi H. Yacoub, Adrian H. Chester, Peter D. Weinberg
Summary: This study investigates the potential role of the transforming growth factor -I3 (TGF-I3) receptor ALK5 in calcific aortic valve disease. The findings suggest that endothelial dysfunction and inflammation induced by low magnitude multidirectional flow can affect ALK5 mechanosensory signaling, leading to increased endothelial leukocyte adhesion and paracellular permeability. ALK5 may play an important role in the pathogenesis of shear stress-associated cardiovascular disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed, Mariona Graupera, Gergana Dobreva, Martin A. Schwartz, Roxana Ola
Summary: This study aims to elucidate the mechanism of synergy between fluid shear stress (FSS) and SMAD signaling in vascular stability and how disruption of this synergy leads to arterial-venous malformations (AVMs). It was found that loss of Smad4 increases endothelial cell sensitivity to flow, resulting in AVMs with excessive flow-mediated morphological responses. Mechanistically, loss of SMAD4 disinhibits flow-mediated KLF4-TIE2-PI3K/Akt signaling, leading to loss of arterial identity due to KLF4-mediated repression of CDK inhibitors CDKN2A and CDKN2B.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Zongkui Wang, Peng Jiang, Fengjuan Liu, Xi Du, Li Ma, Shengliang Ye, Haijun Cao, Pan Sun, Na Su, Fangzhao Lin, Rong Zhang, Changqing Li
Summary: This study reveals that GDF11 negatively regulates neurogenesis by activating the ALK5-dependent PI3K-Akt signaling pathway in neural stem cells, inhibiting cell proliferation and migration while promoting differentiation and apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Lei Wang, Yukun Wang, Bijun Li, Yiyu Zhang, Shuaifei Song, Wenfei Ding, Dejun Xu, Zhongquan Zhao
Summary: This study found that bone morphogenetic protein 6 (BMP6) can promote the secretion and expression of anti-Mullerian hormone (AMH) in goat ovarian granulosa cells (GCs). Mechanistically, BMP6 upregulates the expression of sex-determining region Y-box 9 (SOX9) and GATA-binding factor 4 (GATA4), which leads to the transcriptional initiation of AMH.
Article
Neurosciences
Hemalatha Muralidharan, Shrobona Guha, Kiran Madugula, Ankita Patil, Sarah A. Bennison, Xiaohuan Sun, Kazuhito Toyo-oka, Peter W. Baas
Summary: This study reveals the essential role of KIFC1 in ensuring the accuracy of neuronal migration trajectory. By crosslinking microtubules into a nonsliding mode, KIFC1 enables dynein-driven forces to achieve sufficient traction and thus allows neurons to migrate along their appropriate path.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Senem Aykul, Jordan Maust, Vijayalakshmi Thamilselvan, Monique Floer, Erik Martinez-Hackert
Summary: The study revealed the significant regulatory role of TGF-beta family growth factors and inhibitors in adipocyte development, with SMAD1/5/8 signaling playing a crucial role in 3T3-L1 adipogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Ling Guo, Xiaoqing Wen, Yinglong Hou, Rong Sun, Liang Zhang, Fuhong Liu, Ju Liu
Summary: Anti-angiogenesis therapy is a new treatment method for malignant tumors, and dihydroartemisinin (DHA) inhibits endothelial cell (EC) migration through the TGF-beta 1/ALK5/SMAD2 signaling pathway. These findings suggest that DHA may be a novel drug for the treatment of patients with malignant tumors.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Developmental Biology
Lanlan Fang, Zhen Wang, Ze Wu, Yang Yan, Yibo Gao, Yuxi Li, Jung-Chien Cheng, Ying-Pu Sun
Summary: The study revealed the promotive effect of GDF-8 on MMP2 expression in human trophoblast cells, mediated through the AUKS-SMAD2/3 signaling pathway. Knockdown of MMP2 attenuated the GDF-8-induced cellular invasiveness.
Article
Cell Biology
Christoph J. Burckhardt, John D. Minna, Gaudenz Danuser
Summary: The research demonstrates that SH3BP4 regulates integrin surface expression in a signaling-dependent manner, impacting cell-matrix interactions. Decreased Akt kinase activity leads to SH3BP4 mediating endocytosis of neuropilin-1 and alpha 5/beta 1-integrins, inhibiting cell spreading.
DEVELOPMENTAL CELL
(2021)
Article
Biology
Campbell D. Lawson, Samantha Peel, Asier Jayo, Adam Corrigan, Preeti Iyer, Mabel Baxter Dalrymple, Richard J. Marsh, Susan Cox, Isabel Van Audenhove, Jan Gettemans, Maddy Parsons
Summary: Fascin is an important regulator of F-actin bundling in tumor cells. It has been found that Fascin bundles F-actin within the nucleus, supporting chromatin organization and efficient DNA damage response. Increased nuclear accumulation of Fascin leads to sustained nuclear F-actin bundling, resulting in reduced invasion, viability, and apoptosis of cancer cells.
Article
Biochemistry & Molecular Biology
Ying Zhang, Huanyu Zheng, Ming Xu, Noriko Maeda, Ryouichi Tsunedomi, Hiroko Kishi, Hiroaki Nagano, Sei Kobayashi
Summary: Metastasis is the leading cause of death in breast cancer patients. Elevated levels of paxillin expression and its tyrosine phosphorylation have been shown to play a critical role in breast cancer cell migration and invasion. This study reveals that paxillin overexpression is associated with poor prognosis in breast cancer patients. It also demonstrates that Fyn-mediated phosphorylation of paxillin tyrosine 31 is required for breast cancer migration and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jennifer Chean, Charng-Jui Chen, Gabriel Gugiu, Patty Wong, Seung Cha, Harry Li, Tung Nguyen, Supriyo Bhatticharya, John E. Shively
Summary: This study investigated the impact of CEACAM1 protein and its mutations on lipid regulation, finding that the Ser508Ala mutation led to decreased lipid storage in both mice and human HepG2 cells, highlighting evolutionary changes between the CEACAM1 genes in mice and humans.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Marco A. Alfonzo-Mendez, Kem A. Sochacki, Marie-Paule Strub, Justin W. Taraska
Summary: The study reveals the interaction between growth factor signaling and cell adhesion, and highlights the role of flat clathrin lattices in enhancing signal transduction.
NATURE COMMUNICATIONS
(2022)
Article
Dermatology
Yuliang He, Jihye Kim, Carlotta Tacconi, Jonathan Moody, Lothar C. Dieterich, Florian Anzengruber, Julia-Tatjana Maul, Epameinondas Gousopoulos, Gaetana Restivo, Mitchell P. Levesque, Nicole Lindenblatt, Jay W. Shin, Chung-Chau Hon, Michael Detmar
Summary: This study used single-cell RNA sequencing to investigate the transcriptomic alterations in blood vascular and lymphatic endothelial cells from psoriatic and healthy human skin. The results showed specific molecular repatterning and dysregulated gene regulatory networks in different subpopulations of endothelial cells in psoriasis. These findings provide insights into the specific transcriptional responses and regulatory mechanisms of endothelial cells in chronic skin inflammation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Nuria Gallisa-Sune, Paula Sanchez-Fernandez-de-Landa, Fabian Zimmermann, Marina Serna, Laura Regue, Joel Paz, Oscar Llorca, Jens Luders, Joan Roig
Summary: This study demonstrates that the adaptor protein BICD2 is activated through phosphorylation, which promotes the formation of the dynein motor complex and regulates its localization and activity within cells. This finding is crucial for understanding the functional importance of dynein in both dividing and non-dividing cells.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hongyu Tian, Jennifer J. Huang, Christelle Golzio, Xia Gao, Melissa Hector-Greene, Nicholas Katsanis, Gerard C. Blobe
Meeting Abstract
Oncology
Manisha Palta, Brian G. Czito, Eileen Duffy, Mary Malicki, Donna Niedzwiecki, James L. Abbruzzese, Hope Elizabeth Uronis, Gerard C. Blobe, Dan G. Blazer, Christopher Willett
JOURNAL OF CLINICAL ONCOLOGY
(2018)
Meeting Abstract
Oncology
S. J. Stephens, B. Czito, X. Zhang, E. Duffy, M. Malicki, B. Pitcher, D. Niedzwiecki, J. Abbruzzese, H. Uronis, G. Blobe, D. G. Blazer, C. G. Willett, M. Palta
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2018)
Article
Biochemistry & Molecular Biology
Jennifer J. Huang, Armando L. Corona, Brian P. Dunn, Elise M. Cai, Jesse N. Prakken, Gerard C. Blobe
Article
Oncology
Jeffrey Melson Clarke, Gerard C. Blobe, John H. Strickler, Hope Elizabeth Uronis, S. Yousuf Zafar, Michael Morse, Evan Dropkin, Leigh Howard, Margot O'Neill, Christel N. Rushing, Donna Niedzwiecki, Hollie Watson, Emily Bolch, Christy Arrowood, Yingmiao Liu, Andrew B. Nixon, Herbert I. Hurwitz
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2019)
Meeting Abstract
Oncology
C. L. Kent, D. Marin, D. Niedzwiecki, S. J. Stephens, E. Duffy, M. Malicki, J. Abbruzzese, H. Uronis, G. Blobe, D. G. Blazer, B. Czito, C. G. Willett, M. Palta
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2019)
Meeting Abstract
Oncology
Hope Elizabeth Uronis, Christel Rushing, Gerard C. Blobe, Shiaowen David Hsu, Niharika B. Mettu, James Leroy Wells, Donna Niedzwiecki, Leighanne Hartman, Ashley Moyer, Herbert Hurwitz, John H. Strickler
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Oncology
John H. Strickler, Christel N. Rushing, Hope E. Uronis, Michael A. Morse, Donna Niedzwiecki, Gerard C. Blobe, Ashley N. Moyer, Emily Bolch, Renee Webb, Sherri Haley, Ace J. Hatch, Ivy P. Altomare, Gary B. Sherrill, David Z. Chang, James L. Wells, S. David Hsu, Jingquan Jia, S. Yousuf Zafar, Andrew B. Nixon, Herbert I. Hurwitz
Summary: The combination of cabozantinib and panitumumab has shown activity in patients with metastatic colorectal cancer, with dose modifications of cabozantinib improving tolerability. Further study is warranted to identify biomarkers for patient populations most likely to benefit.
Article
Cell Biology
Keren Tazat, Leslie Pomeraniec-Abudy, Melissa Hector-Greene, Szabina Szofia Szilagyi, Swati Sharma, Elise M. Cai, Armando L. Corona, Marcelo Ehrlich, Gerard C. Blobe, Yoav Henis
Summary: Research demonstrates that ALK1, endoglin, and T beta RIII form stable complexes at the cell surface and regulate the endocytosis of TGF-beta receptors. Among these complexes, ALK1 plays a major role in affecting the endocytosis of T beta RIII and endoglin. Additionally, endoglin has minimal impact on the endocytosis of ALK1, ALK5, or T beta RII, while T beta RIII slows the internalization of ALK5 and T beta RII.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Review
Anatomy & Morphology
John B. Pawlak, Gerard C. Blobe
Summary: TGF-beta superfamily signaling is often modified by co-receptors, leading to dynamic effects on SMAD-mediated pathways. The release of soluble forms of these co-receptors can further modulate signaling, potentially antagonizing membrane-bound receptors.
DEVELOPMENTAL DYNAMICS
(2022)
Review
Cell Biology
Armando Corona, Gerard C. Blobe
Summary: TGFBI plays a dual role in cancer progression, acting as a tumor suppressor in the context of hereditary corneal dystrophies and promoting tumor progression in established tumors. Targeting TGFBI may have potential clinical utility in treating advanced cancers and assessing TGFBI levels could serve as a biomarker for chemotherapy resistance and tumor progression.
CELLULAR SIGNALLING
(2021)
Meeting Abstract
Oncology
D. J. George, E. Moore, G. C. Blobe, N. DeVito, B. A. Hanks, M. R. Harrison, C. J. Hoimes, J. Jia, M. Morse, P. Jayaprakasan, A. MacKelfresh, H. Mulder, K. Beauchamp, J. Michuda, M. C. Stumpe, E. Perakslis, T. Taxter
ANNALS OF ONCOLOGY
(2022)
Article
Oncology
Elle C. Moore, Gerard C. Blobe, Nicholas C. Devito, Brent A. Hanks, Michael R. Harrison, Christopher J. Hoimes, Jingquan Jia, Michael A. Morse, Parvathy Jayaprakasan, Andrew MacKelfresh, Hillary Mulder, Adam J. Hockenberry, Alia Zander, Martin C. Stumpe, Jackson Michuda, Kyle A. Beauchamp, Eric Perakslis, Timothy Taxter, Daniel J. George
Summary: This retrospective study evaluated the utility of a novel molecular diagnostic classifier in changing treatment recommendations for patients with cancer of uncertain origin. The results showed that the molecular diagnostic classifier altered treatment plans in the majority of patients, increasing confidence in selecting the most appropriate treatment regimen. Further studies are needed to determine if the use of molecular diagnostic classifiers improves clinical outcomes for patients with cancer of unknown primary.
Meeting Abstract
Peripheral Vascular Disease
Melissa Hector-Greene, Keren Tazat, Yoav Henis, Gerard Blobe
Meeting Abstract
Peripheral Vascular Disease
Hongyu Tian, Tatiana Ketova, Duriel Hardy, Xiaojiang Xu, Xia Gao, Andries Zijlstra, Gerard C. Blobe