Article
Cell Biology
Chika Ogura, Shoko Nishihara
Summary: This study demonstrated the important role of DS in maintaining the undifferentiated state of mESCs and influencing their differentiation direction and Wnt signaling pathway. Knockdown or overexpression experiments targeting the D4ST1 gene revealed the mechanism of DS in mESCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pei Lu, Mao Li, Donghui Zhang, Wei Jiang
Summary: Long noncoding RNAs (lncRNAs) play important roles in regulating cell fate in pluripotent stem cells. They have four major functional mechanisms based on different types of cofactors. Further understanding of lncRNA-based regulation will provide more insights into the drivers manipulating cell fate.
Article
Biochemistry & Molecular Biology
Martin Hsiu-Chu Lin, Ping-Shan Lai, Li-Ching Chang, Wei-Chao Huang, Ming-Hsueh Lee, Kuo-Tai Chen, Chiu-Yen Chung, Jen-Tsung Yang
Summary: The study combines PS80 PBCA NP with chitosan surface modification for the transfection-guided neural-lineage specification of mouse iPSCs. The complex exhibits high stability, low cell toxicity, and enhances BDNF expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Roman Goetzke, Giulio Abagnale, Burcu Yesilyurt, Lucia Salz, Olivia Cypris, Philipp Glueck, Sven Liesenfelder, Kira Zeevaert, Zhiyao Ma, Ronghui Li, Ivan G. Costa, Angelika Lampert, Vivek Pachauri, Uwe Schnakenberg, Martin Zenke, Wolfgang Wagner, Mohamed H. Elsafi Mabrouk, Marcelo A. S. Toledo
Summary: Colonies of induced pluripotent stem cells (iPSCs) exhibit self-organization and transition into 3D aggregates. This transition is characterized by gradual upregulation of specific genes and the formation of embryoid bodies (EBs). The use of polydimethylsiloxane (PDMS) pillars or micro-contact printing of vitronectin plays a role in spatial confinement and the formation of controlled size EBs without enzymatic or mechanical treatment.
Review
Chemistry, Multidisciplinary
Sajedeh Nasr Esfahani, Agnes M. Resto Irizarry, Xufeng Xue, Samuel Byung-Deuk Lee, Yue Shao, Jianping Fu
Summary: Research on controlling the fate of human pluripotent stem cells is challenging, but micro/nanoengineering technologies bring new hope. Biochemical and biomechanical cues play crucial roles in determining the behavior of human pluripotent stem cells.
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Engineering, Biomedical
Yanxi Liu, Shraddha Chakraborty, Chatrawee Direksilp, Johannes M. Scheiger, Anna A. Popova, Pavel A. Levkin
Summary: The study demonstrates the feasibility of culturing hiPSCs in 200 nL droplets on DMA, showing that they can attach to the MG-free surface and maintain good viability without spontaneous differentiation. Additionally, culturing hiPSCs in 200 nL droplets leads to higher expression of the pluripotency marker Nanog compared to 2 mL culture.
MATERIALS TODAY BIO
(2021)
Article
Biochemistry & Molecular Biology
Anastasiya Mamaeva, Olga Krasnova, Irina Khvorova, Konstantin Kozlov, Vitaly Gursky, Maria Samsonova, Olga Tikhonova, Irina Neganova
Summary: Human pluripotent stem cells have great potential for research and therapy, but their maintenance in culture requires careful control of their pluripotent and clonal status. In this study, we developed a classifier using a convolutional neural network to analyze images of human embryonic stem cell colonies and predict their morphological phenotypes associated with pluripotency and clonality. The classifier showed high accuracy in predicting phenotype (89%) and the spatial scale of around 144 μm was found to be most informative for classification. Proteomic analysis also revealed molecular differences between cells with different phenotypes. This non-invasive automated analysis approach could be valuable for identifying developmental anomalies in pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maki Takeda, Emiko Ito, Kazumasa Minami, Akima Harada, Noriko Mochizuki-Oda, Yoshiki Sawa, Shigeru Miyagawa
Summary: Irradiation of hiPSC-CMs can reduce the number of undifferentiated hiPSCs and tumor formation risk, without affecting the growth and morphology of cardiomyocytes. Tumorigenicity tests in mice showed lower tumor formation frequency in the irradiation group compared to the control group.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Ping Zheng
Summary: Pluripotent stem cells have wide applications, but the mechanisms of maintaining genome stability in these cells are not clear.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Biology
Chengyan Chen, Yanmin Shen, Luqian Li, Yaoxin Ren, Zhao-Qi Wang, Tangliang Li
Summary: Nonsense-mediated mRNA decay (NMD) is a highly conserved regulatory mechanism in eukaryotic cells, and UPF3A and UPF3B are key factors in this process. This study found that UPF3A does not repress or promote NMD in mouse embryonic stem cells, somatic cells, and major organs when UPF3B is present.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Weixiang Song, Danchen Zhang, Jiaqi Mi, Wenfei Du, Yang Yang, Rong Chen, Cong Tian, Xiaodong Zhao, Kang Zou
Summary: E-cadherin and Cadherin-22 synergistically maintain the undifferentiation of SPCs through the pivotal intermediate molecule beta-catenin. LEF1, TCF3, STAT3, and HDAC4 were identified as co-regulatory factors of beta-catenin in regulating SPC fate.
CELL AND BIOSCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Kazunori Bando, Hiromi Yamashita, Motomu Tsumori, Hayase Minoura, Koji Okumura, Fumiyuki Hattori
Summary: This study developed an all-in-one automated culture machine that can efficiently expand human pluripotent stem cells and differentiate them into various cell types. This would facilitate the social implementation of human pluripotent stem cells and contribute to the standardization of the entire handling processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Peter Chovanec, Amanda J. Collier, Christel Krueger, Csilla Varnai, Claudia I. Semprich, Stefan Schoenfelder, Anne E. Corcoran, Peter J. Rugg-Gunn
Summary: The authors generated a high-resolution atlas of gene regulatory interactions, chromatin profiles, and transcription factor occupancy in naive and primed human pluripotent stem cells. The study provides insights into the mechanisms underlying the transition between naive and primed pluripotency states.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Guillermo Galdon, Nicholas A. Deebel, Nima Pourhabibi Zarandi, Mark J. Pettenati, Stanley Kogan, Christina Wang, Ronald S. Swerdloff, Anthony Atala, Yanhe Lue, Hooman Sadri-Ardekani
Summary: This study provides the first evidence of the loss of an extra sex chromosome during innate SSC culture, which is crucial for treating Klinefelter syndrome (KS) patients and preserving and propagating SSCs for future sperm production, either in vitro or in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Reika Makita, Tsukasa Akasaka, Seiichi Tamagawa, Yasuhiro Yoshida, Saori Miyata, Hirofumi Miyaji, Tsutomu Sugaya
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
(2018)
Article
Materials Science, Biomaterials
Tsukasa Akasaka, Atsuro Yokoyama, Makoto Matsuoka, Takeshi Hashimoto, Fumio Watari
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2010)
Article
Materials Science, Biomaterials
Makoto Matsuoka, Tsukasa Akasaka, Yasunori Totsuka, Fumio Watari
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2012)
Article
Materials Science, Multidisciplinary
Tsukasa Akasaka, Makoto Matsuoka, Takeshi Hashimoto, Shigeaki Abe, Motohiro Uo, Fumio Watari
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
(2010)
Article
Materials Science, Multidisciplinary
Makoto Matsuoka, Tsukasa Akasaka, Yasunori Totsuka, Fumio Watari
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
(2010)
Article
Engineering, Biomedical
Naoyuki Kaga, Tsukasa Akasaka, Takashi Matsuura, Atsuro Yokoyama, Yasuhiro Yoshida
BIO-MEDICAL MATERIALS AND ENGINEERING
(2020)
Article
Chemistry, Physical
Tsukasa Akasaka, Miho Tamai, Yoshitaka Yoshimura, Natsumi Ushijima, Shinichiro Numamoto, Atsuro Yokoyama, Hirofumi Miyaji, Ryo Takata, Shuichi Yamagata, Yoshiaki Sato, Ko Nakanishi, Yasuhiro Yoshida
Summary: The surface topography of a material can influence osteoclast activity. Pillar-shaped patterns are the most effective in inducing osteoclastogenesis. The generation of osteoclasts can be controlled by designing surfaces with specific morphologies.
Correction
Chemistry, Physical
Tsukasa Akasaka, Miho Tamai, Yoshitaka Yoshimura, Natsumi Ushijima, Shinichiro Numamoto, Atsuro Yokoyama, Hirofumi Miyaji, Ryo Takata, Shuichi Yamagata, Yoshiaki Sato, Ko Nakanishi, Yasuhiro Yoshida
Summary: The article examines how different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure.
Article
Nanoscience & Nanotechnology
J. Honma, T. Akasaka, M. Tamai, Y. Yoshimura, T. Taira, H. Miyaji, S. Yamagata, Y. Sato, Y. Yoshida
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
(2018)
Article
Nanoscience & Nanotechnology
R. Takata, T. Akasaka, M. Tamai, Y. Yoshimura, T. Taira, H. Miyaji, Y. Tagawa, S. Yamagata, J. Iida, Y. Yoshida
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
(2018)
Article
Nanoscience & Nanotechnology
N. Kaga, T. Akasaka, R. Horiuchi, Y. Yoshida, A. Yokoyama
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
(2017)
Article
Nanoscience & Nanotechnology
T. Akasaka, H. Miyaji, T. Imamura, N. Kaga, A. Yokoyama, Y. Yoshida
DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES
(2017)
Article
Nanoscience & Nanotechnology
Tsukasa Akasaka, Takuya Imamura, Hirofumi Miyaji, Naoyuki Kaga, Atsuro Yokoyama, Yasuhiro Yoshida
E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY
(2016)
Proceedings Paper
Materials Science, Ceramics
Tsukasa Akasaka, Shigeaki Abe, Fumio Watari
Article
Chemistry, Physical
Jie Sheng, Jingshan He, Dun Ma, Yuanbo Wang, Wu Shao, Tian Ding, Ronghao Cen, Jingwen He, Zhihao Deng, Wenjun Wu
Summary: This study presents an innovative approach to improve the photovoltaic conversion characteristics and stability of perovskite solar cells through carbon electrode interface modification. By in-situ polymerization and carbonization on the surface of nano-graphite, a dendritic structure carbon electrode is formed, reducing the work function and aligning the energy levels with perovskite. This leads to improved charge and hole collection efficiency, resulting in increased photovoltaic conversion efficiency. Furthermore, the modified carbon electrode-based perovskite solar cells exhibit exceptional stability, maintaining high efficiency even without encapsulation.
Article
Chemistry, Physical
Guodong Shi, Jian Song, Xiaoxiao Tian, Tongtong Liu, Zhanjun Wu
Summary: This study demonstrates the improvement of mechanical properties and reduction of coefficient of thermal expansion (CTE) in graphene oxide (GO)/epoxy (EP) nanocomposites by enhancing the interface between GO and EP through functionalization and incorporating rigid-flexible interphases. The results reveal that the SiO2-PEA-GO hybrid exhibits better strengthening and toughening effects, as well as lower CTE, compared to the PEA-GO hybrid due to the presence of rigid-flexible interfaces with higher bonding strength and better energy dissipation mechanisms. Additionally, the nanocomposites with longer polyetheramine (PEA) molecules in the rigid-flexible interphases demonstrate higher strength and toughness, while maintaining a lower CTE. This work provides a promising strategy for constructing adjustable flexible-rigid interfacial structures and offers potential in developing GO/EP nanocomposites with high mechanical properties and low CTE.
Article
Chemistry, Physical
Rafal Janus, Sebastian Jarczewski, Jacek Jagiello, Piotr Natkanski, Mariusz Wadrzyk, Marek Lewandowski, Marek Michalik, Piotr Kustrowski
Summary: In this study, a facile procedure for the synthesis of CMK-1 and CMK-2 carbon replicas was developed. The method utilizes basic laboratory equipment and a renewable carbon source, and operates under mild conditions. The resulting carbon mesostructures exhibit exquisite replication fidelity and structural homogeneity, making them suitable for applications in various fields.
Article
Chemistry, Physical
Anqi Wang, Connor J. MacRobbie, Alex Baranovsky, Jean-Pierre Hickey, John Z. Wen
Summary: In this study, a novel polymer-free nanothermite aerogel with a wide range of nanoparticle loading was fabricated via a new additive manufacturing process. The SEM images showed a unique porous structure formed by extra thin rGO sheets, wrapping individual nanothermite clusters. The DSC-TGA results and high-speed combustion videos confirmed the enhanced energetic performance of the printed specimen.
Article
Chemistry, Physical
Wanze Wu, Misheng Zhao, Shiwei Miao, Xiaoyan Li, Yongzhong Wu, Xiao Gong, Hangxiang Wang
Summary: Superhydrophobic solar-driven interfacial evaporator is an energy-efficient technology for seawater desalination, which is easily fabricated using robust photothermal superhydrophobic coating and substrate. The created bifunctional coating on the melamine sponge substrate shows stable and highly efficient photothermal and superhydrophobic performance for seawater desalination. This superhydrophobic solar-driven interfacial evaporator is expected to have wide applications in seawater desalination.
Article
Chemistry, Physical
Zichen Xiang, Zhi Song, Tiansheng Wang, Menghang Feng, Yijing Zhao, Qitu Zhang, Yi Hou, Lixi Wang
Summary: This study presents a co-electrospinning synthesis strategy to fabricate lightweight and porous Co@C composite nanofibres with wideband microwave attenuation capacity. The addition of MOF-derived Co additives enhances the low-frequency absorption performance.
Article
Chemistry, Physical
J. Snow, C. Olson, E. Torres, K. Shirley, E. Cazalas
Summary: This study investigates the use of a perovskite-based graphene field effect transistor (P-GFET) device for X-ray detection. The sensitivity and responsivity of the device were found to be influenced by factors such as X-ray tube voltage, current, and source-drain voltage. Simulation experiments were conducted to determine the dose rate and energy incident on the device during irradiation.
Article
Chemistry, Physical
Zuzana Jankovska, Lenka Matejova, Jonas Tokarsky, Pavlina Peikertova, Milan Dopita, Karolina Gorzolkova, Dominika Habermannova, Michal Vastyl, Jakub Belik
Summary: This study provides new insights into microwave-assisted pyrolysis of scrap tyres, demonstrating that it can produce microporous carbon black with potential application in xylene adsorption. Compared to conventional pyrolysis, microwave pyrolysis requires less time and energy while maintaining similar adsorption capacity.
Article
Chemistry, Physical
Max Bommert, Bruno Schuler, Carlo A. Pignedoli, Roland Widmer, Oliver Groning
Summary: A detailed understanding of the interaction between molecules and two-dimensional materials is crucial for incorporating functional molecular films into next-generation 2D material-organic hybrid devices. This study compares the energy level alignment of different-sized fullerenes on a Moire superstructure and finds that C-84 fullerenes can be either neutral or negatively charged depending on slight variations of the electrostatic potential. This discovery suggests a new path to achieve ambipolar charge transfer without overcoming the electronic gap of fullerenes.
Article
Chemistry, Physical
Yuanjing Cheng, Xianxian Sun, Ye Yuan, Shuang Yang, Yuanhao Ning, Dan Wang, Weilong Yin, Yibin Li
Summary: The dual-structure aerogel (GS) consisting of flexible silica fibers and graphene honeycomb structures exhibits excellent resilience, flexibility, and reliability. It also shows remarkable wave absorbing performance, making it an ideal candidate for microwave absorption applications such as flexible electronics and aerospace.
Article
Chemistry, Physical
Shuyu Fan, Yinong Chen, Shu Xiao, Kejun Shi, Xinyu Meng, Songsheng Lin, Fenghua Su, Yifan Su, Paul K. Chu
Summary: Graphene coatings are promising solid lubrication materials due to their mechanical properties. This study presents a new method for in situ deposition of high-quality graphene coatings on hard substrates using NiCo solid solution and competitive reaction strategies. The graphene coating deposited on substrates with deep NiCo solid solution demonstrates superior low-friction and durability.
Article
Chemistry, Physical
Mengdi Wang, Sanyin Qu, Yanling Chen, Qin Yao, Lidong Chen
Summary: The improved thermoelectric properties of conducting polymers are achieved by selectively capturing single-walled carbon nanotubes (SWNTs) in a conducting polymer film, leading to increased carrier mobility and reduced thermal conductivity. The resulting composite film exhibits significantly higher electrical conductivity and lower thermal conductivity compared to films with a mixture of SWNTs. This work provides a convenient and efficient method to enhance the thermoelectric properties of conducting polymers.
Review
Chemistry, Physical
Heng Wei, Weihua Li, Kareem Bachagha
Summary: This article reviews the research progress of carbon nanotube-based microwave absorbing materials (MAMs) in recent years, covering the fundamental theory, design strategies, synthesis methods, and future development directions.
Article
Chemistry, Physical
Chenguang Shi, Junlong Huang, Zongheng Cen, Tan Yi, Shaohong Liu, Ruowen Fu
Summary: This study developed a high-performance Li metal host material, which achieved dendrite-free Li deposition with a low nucleation overpotential and high Coulombic efficiencies through the combination of Ti3C2-g-PV4P sheets and Ag nanoparticles. The full cells assembled with the Li@host anode and LiFePO4 cathode exhibited high discharge capacity and excellent cycling stability, demonstrating a perspective design for future energy storage devices.
Article
Chemistry, Physical
Tomotaro Mae, Kentaro Kaneko, Hiroki Sakurai, Suguru Noda
Summary: A new partial prelithiation method for SiO/C-CNT electrodes was developed, which showed reduced irreversible capacity and achieved high energy densities with good reversibility. The method allows for precise control of the degree of prelithiation and is applicable to various chemistries.