期刊
CARBOHYDRATE RESEARCH
卷 371, 期 -, 页码 45-51出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2013.02.004
关键词
Hybrid mushroom; Heteroglycan; NMR experiments; Structural characterization; Immunoactivation
资金
- CSIR, India [CSIR-09/599(042)/2011-EMR-I]
An immunostimulating water-soluble heteroglycan (PS-II) was isolated from an aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h produced by intergeneric protoplast fusion between Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-II was carried out using sugar analysis, methylation experiment, periodate oxidation and 1D/2D NMR studies. Sugar analysis indicated the presence of glucose, mannose and galactose in a molar ratio of 1:1:2. Methylation analysis revealed that PS-II was composed of (1 -> 6)- and (1 -> 2,4,6)-alpha-D-galactopyranosyl, terminal beta-D-mannopyranosyl and terminal beta-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. On the basis of chemical analysis and NMR studies, the structure of the repeating unit of the heteroglycan was established to consist of a backbone chain of two (1 -> 6)-alpha-D-galactopyranosyl residues, one of which is branched at O-2 with terminal beta-D-Manp and at O-4 with terminal beta-D-Glcp. This heteroglycan (PS-II) showed in vitro splenocyte, thymocyte and macrophage activations. (C) 2013 Elsevier Ltd. All rights reserved.
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