期刊
CARBOHYDRATE RESEARCH
卷 361, 期 -, 页码 170-174出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2012.09.001
关键词
Carnivorous plant; Class III; Chitinase; GH family 18; Nepenthes
A class III chitinase belonging to the GH18 family from Nepenthes alata (NaCHIT3) was expressed in Escherichia coli. The enzyme exhibited hydrolytic activity toward colloidal chitin, ethylene glycol chitin, and (GlcNAc)(n) (n = 5 and 6). The enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)(6). The anomeric form of the products indicated it was a retaining enzyme. The colloidal chitin hydrolytic reaction displayed high activity between pH 3.9 and 6.9, but the pH optimum of the (GlcNAc)(6) hydrolytic reaction was 3.9 at 37 degrees C. The optimal temperature for activity was 65 degrees C in 50 mM sodium acetate buffer (pH 3.9). The pH optima of NaCHIT3 and NaCHIT1 might be related to their roles in chitin degradation in the pitcher fluid. (C) 2012 Elsevier Ltd. All rights reserved.
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