Article
Biochemistry & Molecular Biology
Dallas R. Fonseca, Madison B. Loppnow, Leslie A. Day, Elisa L. Kelsey, Mohd Farid Abdul Halim, Kyle C. Costa
Summary: Natural transformation is an important evolutionary driver in microbial populations, but its mechanism is mainly characterized in bacteria. In this study, we identified a catalog of genes necessary for transformation in Methanococcus maripaludis, expanding our understanding of natural transformation in archaea. We found several transformation-associated candidate genes, including those encoding components of the type IV-like pilus, transcription/translation associated genes, putative membrane-bound transport proteins, and genes with unknown functions. Furthermore, we confirmed the importance of these genes for transformation using allelic replacement mutagenesis, and identified a conserved mechanism for DNA transfer in multiple archaea.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Qing Xu, Qing Du, Jian Gao, Lei Chen, Xiuzhu Dong, Jie Li
Summary: Researchers developed a genetic toolbox including libraries of promoters, ribosome binding sites, and neutral sites for precise gene expression in M. maripaludis. They successfully improved recombinant protein expression and modulated essential gene expression, establishing a solid foundation for further studies and biotechnological applications of M. maripaludis.
METABOLIC ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Kohtaro Hirao, Immacolata Speciale, Anna Notaro, Yoshiyuki Manabe, Yoshiaki Teramoto, Takaaki Sato, Haruyuki Atomi, Antonio Molinaro, Yoshihiro Ueda, Cristina De Castro, Koichi Fukase
Summary: This study describes the isolation, structural determination, and chemical synthesis of the N-glycan from the hyperthermophilic archaeon Thermococcus kodakarensis, revealing a unique structure including myo-inositol not found in previously characterized N-glycans. The synthesis of this glycan was successfully achieved through diastereoselective phosphorylation and phosphodiester construction by S(N)2 coupling. This research is expected to provide insights into the primitive function of glycan modification at the molecular level by considering the early divergence of hyperthermophilic organisms in evolution.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Zlata Vershinin, Marianna Zaretsky, Ziqiang Guan, Jerry Eichler
Summary: This study reexamined the composition of N-linked glycans in Halobacterium salinarum, revealing a tetrasaccharide composition. Additionally, the absence of oligosaccharyltransferase AglB led to the accumulation of methylated and disulfated tetrasaccharides linked to dolichol phosphate. These findings contribute to a better understanding of the N-glycosylation pathway in this organism.
Article
Biotechnology & Applied Microbiology
Matthew P. Holten, Dallas R. Fonseca, Kyle C. Costa
Summary: The study demonstrates the essential role of AgIB in surface growth of M. maripaludis JJ, with glycosylation of the cell surface layer enhancing anaerobic iron corrosion rates. These findings provide insights into the physiology of surface-associated methanogenic organisms and underscore the importance of surface association for anaerobic iron corrosion.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
John Kelly, Evgeny Vinogradov, Anna Robotham, Luc Tessier, Susan M. Logan, Ken F. Jarrell
Summary: This study reports the N- and O-glycosylation modifications in Methanoculleus marisnigri, a methanogen from the Order Methanomicrobiales. Mass spectrometry and NMR analysis revealed the presence of N- and O-glycans in the S-layer protein, but not in the archaellins. The findings provide insights into the glycosylation diversity in archaea.
Article
Biochemistry & Molecular Biology
Anna Notaro, Marianna Zaretsky, Antonio Molinaro, Cristina De Castro, Jerry Eichler
Summary: Archaea are unique microorganisms that can survive in harsh environments. They have the ability to perform N-glycosylation, which exhibits high diversity in terms of sugar composition and structure.
CARBOHYDRATE RESEARCH
(2023)
Article
Biochemical Research Methods
Xiaoyang Hou, Yu Wang, Dongbo Bu, Yaojun Wang, Shiwei Sun
Summary: N-linked glycosylation is a crucial protein modification involved in various biological processes. The EMNGly approach, which combines machine learning and deep learning, improves glycosylation site prediction accuracy and outperforms existing techniques.
Article
Chemistry, Analytical
James W. Dressman, Colin T. McDowell, Xiaowei Lu, Peggi M. Angel, Richard R. Drake, Anand S. Mehta
Summary: N-linked glycosylation plays a crucial role in immune response and cell-to-cell interactions. The current analytical techniques for studying immune cell N-glycosylation have limitations in throughput, structural information, starting materials, and cell purification. This study proposes a rapid antibody array-based approach coupled with MALDI-IMS for analyzing cellular N-glycosylation, which provides a valuable tool for glycoimmunology research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Erika Staudacher
Summary: Molluscs exhibit a highly diverse N-glycan pattern on their proteins, surpassing even that of vertebrates in terms of structural features. This review outlines the current understanding of mollusc N-glycan structures, with a focus on the functional significance of the corresponding glycoproteins. Additionally, it discusses the potential medical applications of biomolecules derived from molluscs, highlighting the significance of mollusc research.
Article
Virology
L. Johanna Gebhard, Zlata Vershinin, Tomas Alarcon-Schumacher, Jerry Eichler, Susanne Erdmann
Summary: N-glycosylation is crucial for cell stability, motility, and virus-host interactions in Archaea. The study investigated the impact of compromised N-glycosylation on virus infection dynamics using a mutant strain of Halorubrum lacusprofundi. The results showed that compromised N-glycosylation had different effects on different viruses, highlighting the significance of N-glycosylation in virus-host interactions.
Review
Biochemistry & Molecular Biology
Baoquan Chen, Wenqiang Liu, Yaohao Li, Bo Ma, Shiying Shang, Zhongping Tan
Summary: Therapeutic proteins are increasingly valued for their unique advantages in the treatment of various diseases, but their intrinsic limitations often restrict their wider applications. Glycosylation, as a crucial post-translational modification, has positive effects on protein properties. Glycoengineering, involving changing glycosylation patterns, is expected to be an effective means of improving the performance of therapeutic proteins.
Article
Neurosciences
Jissele A. Verdinez, Julien A. Sebag
Summary: This study identified two N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrated that glycosylation at position 27 is crucial for its plasma membrane localization and signaling. Additionally, glycosylation at position 7 was found to decrease PKR2 signaling through G alpha(s) without affecting G alpha(q/)(11) signaling.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Colin T. McDowell, Zachary Klamer, Johnathan Hall, Connor A. West, Luke Wisniewski, Thomas W. Powers, Peggi M. Angel, Anand S. Mehta, David N. Lewin, Brian B. Haab, Richard R. Drake
Summary: Detecting PDAC early is crucial for patient survival, and current carbohydrate biomarkers show potential for monitoring disease progression. By using imaging mass spectrometry to study the N-glycome of human pancreas and pancreatic cancer, distinct differences were found in tissue samples categorized by different biomarkers.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Immunology
Hanqing Chen, Yang Zhang, Yu Shen, Liang Jiang, Guangbo Zhang, Xueguang Zhang, Yang Xu, Fengqing Fu
Summary: This study reveals the existence of N-glycosylation in the novel immune checkpoint molecule B7-H6. It shows that N43 and N208 glycosylation are essential for the stability and membrane expression of B7-H6. Lack of N208 glycosylation leads to membrane shedding, while N43 mutation impairs the binding affinity with NKp30. These findings highlight the significance of N-linked glycosylation in the biological functions of B7-H6 and suggest potential targets for modulating NK cell-mediated immunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Eleonora Altman, Vandana Chandan, Blair A. Harrison, Evgeny Vinogradov
CARBOHYDRATE RESEARCH
(2018)
Review
Biochemistry & Molecular Biology
Guillaume Goyette-Desjardins, Evgeny Vinogradov, Masatoshi Okura, Daisuke Takamatsu, Marcelo Gottschalk, Mariela Segura
CARBOHYDRATE RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Jose O. Previato, Evguenii Vinogradov, Maria Alice Esteves Silva, Priscila A. Oliveira, Leonardo M. Fonseca, Emmanuel Maes, Lucia Mendonca-Previato
CARBOHYDRATE RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Salim T. Islam, Israel Vergara Alvarez, Fares Saidi, Annick Giuseppi, Evgeny Vinogradov, Gaurav Sharma, Leon Espinosa, Castrese Morrone, Gael Brasseur, Jean-Francois Guillemot, Anais Benarouche, Jean-Luc Bridot, Gokulakrishnan Ravicoularamin, Alain Cagna, Charles Gauthier, Mitchell Singer, Henri-Pierre Fierobe, Tam Mignot, Emilia M. F. Mauriello
Article
Biochemistry & Molecular Biology
John F. Kelly, Evgeny Vinogradov, Jacek Stupak, Anna C. Robotham, Susan M. Logan, Alison Berezuk, Cezar M. Khursigara, Ken F. Jarrell
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Frank St Michael, Perry Fleming, Andrew D. Cox, Evgeny Vinogradov
Summary: The structural analysis of core oligosaccharides from the lipopolysaccharides of several strains of F. nucleatum showed that they all have a common octasaccharide region and specific additional components for each strain. The F. nucleatum core structure is similar to that produced by Aeromonas.
CARBOHYDRATE RESEARCH
(2021)
Article
Microbiology
Yuli Talyansky, Travis B. Nielsen, Jun Yan, Ulrike Carlino-Macdonald, Gisela Di Venanzio, Somnath Chakravorty, Amber Ulhaq, Mario F. Feldman, Thomas A. Russo, Evgeny Vinogradov, Brian Luna, Meredith S. Wright, Mark D. Adams, Brad Spellberg
Summary: The study found that a mobile DNA fragment in a capsule assembly gene led to the high virulence of Acinetobacter baumannii, while repairing the gene resulted in the virulent strain becoming avirulent, with a single sugar being crucial for innate immune cells recognition and phagocytosis. By comparing genomes of A. baumannii strains, a specific gene variation associated with virulence was identified, showing that loss of a single capsule assembly gene dramatically altered virulence by affecting interactions between bacteria and host immune system.
Article
Chemistry, Medicinal
Jeremy A. Duke, Amy Paschall, Lloyd S. Robinson, Cory J. Knoot, Evgeny Vinogradov, Nichollas E. Scott, Mario F. Feldman, Fikri Y. Avci, Christian M. Harding
Summary: This study presents a novel bioconjugation platform for producing a prototype multivalent GBS conjugate vaccine that shows promise in eliciting functional antibodies against GBS capsule. This proof-of-concept research marks the first step towards developing a next-generation multivalent GBS bioconjugate vaccine.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Medicinal
Chantelle M. Cairns, Frank St. Michael, Mohammad Jamshidi, Henk van Faassen, Qingling Yang, Kevin A. Henry, Greg Hussack, Janelle Sauvageau, Evgeny V. Vinogradov, Andrew D. Cox
Summary: The carbohydrate antigen responsible for signals in Pseudomonas aeruginosa has been characterized and shows promise as a vaccine or therapeutic target.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Evgeny Vinogradov, Frank St. Michael, Chantelle Cairns, Andrew D. Cox
Summary: This study reports the structures of the lipopolysaccharide O-chain (OPS) of two strains of Fusobacterium nucleatum, SB-106CP and HM-992, both isolated from cancerous tissues. These strains are associated with colorectal cancer and pregnancy complications. The OPS structure of F. nucleatum 12230, a transtracheal isolate, is also compared, showing differences between the strains.
CARBOHYDRATE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Evgeny Vinogradov, Frank St Michael, Chantelle Cairns, Andrew D. Cox
Summary: Fusobacterium nucleatum is an anaerobic bacterium that causes periodontitis and is also found in colorectal cancer and pregnancy complications. The cell surface structures of the bacterium may be implicated in the pathogenesis.
CARBOHYDRATE RESEARCH
(2022)
Article
Chemistry, Medicinal
Mohammad P. Jamshidi, Chantelle Cairns, Simon Chong, Frank St Michael, Evgeny V. Vinogradov, Andrew D. Cox, Janelle Sauvageau
Summary: Pseudomonas aeruginosa is listed as a priority pathogen by the World Health Organization for the development of new antibiotics. In addition to antibiotics, vaccines could be a valuable tool in combating this bacteria. Researchers have recently identified an immunogenic carbohydrate structure on the cell surface of P. aeruginosa and successfully synthesized immunogenic oligosaccharides, providing a new approach to develop a vaccine against this bacterium.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Fares Saidi, Oscar Javier Gamboa Marin, Jose Ignacio Veytia-Bucheli, Evgeny Vinogradov, Gokulakrishnan Ravicoularamin, Nicolas Y. Jolivet, Ahmad A. Kezzo, Eric Ramirez Esquivel, Adyasha Panda, Gaurav Sharma, Stephane P. Vincent, Charles Gauthier, Salim T. Islam
Summary: The combination of metabolic labeling and dick chemistry allows selective imaging of the surfaces of bacteria. In this study, researchers explored the feasibility of labeling the lipopolysaccharide (LPS) of Myxococcus xanthus using azido analogues of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo). The results show that different azido analogues have varying effects on the growth and motility of the bacteria, providing important insights into the fundamental principles of outer membrane dynamics in live bacterial cells.
Article
Biochemistry & Molecular Biology
Evgeny Vinogradov, Frank St Michael, Andrew D. Cox
Summary: This study reported the lipopolysaccharide O-chain (OPS) structures of three strains of Fusobacterium nucleatum isolated from cancerous tissues. The OPS structure of strain HM-994 was identical to another strain MJR 7757 B, while the OPS structures of strains HM-995 and HM-997 were novel. These findings suggest a high degree of variation in LPS O-antigen structures produced by F. nucleatum, which may pose challenges for serotyping efforts.
CARBOHYDRATE RESEARCH
(2022)
Article
Chemistry, Medicinal
Mohammad P. Jamshidi, Chantelle Cairns, Simon Chong, Frank St. Michael, Evgeny V. Vinogradov, Andrew D. Cox, Janelle Sauvageau
Summary: Pseudomonas aeruginosa, a life-threatening respiratory pathogen, has been added to the World Health Organization's priority list for the development of new antibiotics. In addition to antibiotics, researchers have synthesized a specific carbohydrate structure on the surface of P. aeruginosa and conjugated it to human serum albumin, resulting in an immunogenic vaccine candidate.
ACS INFECTIOUS DISEASES
(2022)
