Review
Biochemistry & Molecular Biology
Shengyang Wang, You Yang, Qian Zhu, Guo-Qiang Lin, Biao Yu
Summary: In this article, we summarize the chemical syntheses of glycans containing over 20 monosaccharide units. The recent syntheses of ultra-long glycans, such as a 92mer arabinogalactan, a 100mer and a 151mer mannan, and a 128mer rhamnomannan, are highlighted. The assembly strategies, glycosylation methods, and protecting group manipulations, which are crucial for successful synthesis, are discussed to provide guidance for the future synthesis of various polysaccharides.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Plant Sciences
Li Tan, Jianfeng Xu, Michael Held, Derek T. A. Lamport, Marcia Kieliszewski
Summary: This study found that Hyp-O-polysaccharides isolated from transgenic Arabidopsis suspension culture have similar structural features to AGPs isolated from tobacco. It also confirmed the presence of a previously identified β-1,6-linkage on the galactan backbone in AGP fusion glycoproteins expressed in tobacco. Furthermore, AGPs expressed in Arabidopsis suspension culture have differences in terminal-rhamnosyl residues and glucuronosylation compared to those expressed in tobacco suspension culture, suggesting the presence of distinct glycosyl transferases and minimum AG structures for type II AG functional features.
Review
Biochemistry & Molecular Biology
Baoquan Chen, Wenqiang Liu, Yaohao Li, Bo Ma, Shiying Shang, Zhongping Tan
Summary: Therapeutic proteins are increasingly valued for their unique advantages in the treatment of various diseases, but their intrinsic limitations often restrict their wider applications. Glycosylation, as a crucial post-translational modification, has positive effects on protein properties. Glycoengineering, involving changing glycosylation patterns, is expected to be an effective means of improving the performance of therapeutic proteins.
Review
Pharmacology & Pharmacy
Kevin Roe
Summary: Several fungal pathogens are resistant to traditional treatments and some have become multidrug-resistant. Alternative treatments such as vaccines, monoclonal antibody injections, or mRNA injections can mask fungal surface proteins and target pathogenic proteins to combat infections. Combining antifungal drugs with mRNA instructions for patient synthesis of conventional or synthetic monoclonal antibodies offer potential advantages over existing treatments.
DRUG DISCOVERY TODAY
(2023)
Article
Plant Sciences
Rui Wang, Anna A. Dobritsa
Summary: In this study, the Arabidopsis mutant tex2 was characterized, showing that the TEX2 gene plays a crucial role in exine development when expressed in the tapetum. Loss of TEX2 leads to abnormal primexine formation and failure of correct sporopollenin assembly. Additionally, tex2 tapetum accumulates metabolic inclusions related to sporopollenin polyketide biosynthesis, providing a potential tool for studying genetic relationships between genes involved in exine formation.
Article
Chemistry, Analytical
Felix Kuhne, Katrin Heinrich, Martin Winter, Juergen Fichtl, Gabriel Hoffmann, Franziska Zaehringer, Katharina Spitzauer, Monika Meier, Tarik A. Khan, Lea Bonnington, Katharina Wagner, Jan Olaf Stracke, Dietmar Reusch, Harald Wegele, Michael Mormann, Patrick Bulau
Summary: Antibody combination therapies have emerged as viable treatment options for severe diseases like cancer. However, the co-formulation production approach poses challenges for the analytical control of drug product quality due to complex variant profiles. This study developed a multi-dimensional liquid chromatography with tandem mass spectrometry method to characterize antibody homo- and hetero-aggregates. The results showed that the co-formulation of trastuzumab and pertuzumab, two monoclonal antibodies with similar properties, exhibited high stability with minimal aggregate formation under storage and stress conditions. The formation of homo- and hetero-aggregates was not significantly affected by formulation and storage conditions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhen Zheng, Min Ma, Yifei Jia, Yusi Cui, Rui Zhao, Shuangshuang Li, Cody Wenthur, Lingjun Li, Gongyu Li
Summary: Conjugate vaccines are a promising strategy for immunotherapeutic intervention in substance use disorder. These vaccines utilize a hapten similar to the target drug, which is conjugated to an immunogenic carrier protein. The antibodies produced provide long-lasting protection against drug overdose by sequestering the drug in the periphery and preventing it from crossing the blood-brain barrier. However, these antibodies exhibit structural heterogeneity, and the relationship between their variations and stability remains unclear. This study presents a rapid mass-spectrometry-based workflow that can assess the conformational heterogeneity and stability of crude antibodies in response to conjugate vaccines.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Adrian Elter, Tina Bock, Dieter Spiehl, Giulio Russo, Steffen C. Hinz, Sebastian Bitsch, Eva Baum, Markus Langhans, Tobias Meckel, Edgar Dorsam, Harald Kolmar, Gerhard Schwall
Summary: This study introduces the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module fused to detection antibodies, which showed improved protein binding capacity and spatial retention compared to naked detection antibodies. The CBM-assisted antibodies were successfully validated in pregnancy detection and SARS-CoV-2 antibody detection LFAs, demonstrating sensitive detection and potential for more sustainable POC diagnostics.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Cell Biology
Jacqueline Cordell
Summary: This article describes the experiences of a laboratory in the application of monoclonal antibodies to diagnostic histochemistry. Important advancements were made in producing monoclonal antibodies, improving immunoenzymatic detection, unmasking antigens, and using synthetic peptides and recombinant proteins. The laboratory also emphasized collaboration and training to spread the expertise of monoclonal production for diagnosis.
Article
Physics, Multidisciplinary
Catia Salvador, Irina Crina Anca Sandu, Erika Sandbakken, Antonio Candeias, A. Teresa Caldeira
Summary: Biocolonization and biodeterioration in Cultural Heritage, particularly in artworks such as paintings, pose significant challenges for conservation strategies and preservation measures. This study analyzed surface-altered Edvard Munch paintings to identify the materials used and understand the associated colonizing microbiota. The use of immunoenzymatic assays and high throughput sequencing revealed the presence of proteolytic bacteria in these artworks, indicating their biodeteriogenic characteristics. Simulation assays further confirmed the degradative action promoted by these proteolytic strains. This research provides valuable insights for effective intervention processes in preserving Munch's paintings.
EUROPEAN PHYSICAL JOURNAL PLUS
(2022)
Article
Chemistry, Analytical
Gang Wu, Chuanfei Yu, Wenbo Wang, Jialiang Du, Zhihao Fu, Gangling Xu, Meng Li, Lan Wang
Summary: Imaged capillary isoelectric focusing (icIEF) and ion exchange chromatography (IEX) are routinely used for charge variant analysis of therapeutic monoclonal antibodies (mAbs). In this study, icIEF-MS and strong cation exchange (SCX)-MS were compared, and it was found that icIEF-MS outperformed SCX-MS in terms of sensitivity, carryover effect, protein identification, and separation resolution.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Immunology
Davide F. Robbiani, Daniel Ruzek
Summary: The NS1 protein of flaviviruses has become a focus of research for its potential in vaccine and immunotherapeutic development. However, a recent study has revealed a potential dark side to NS1, linking it to the development of self-reactive antibodies.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Emanuele Andreano, Ida Paciello, Giulia Piccini, Noemi Manganaro, Piero Pileri, Inesa Hyseni, Margherita Leonardi, Elisa Pantano, Valentina Abbiento, Linda Benincasa, Ginevra Giglioli, Concetta De Santi, Massimiliano Fabbiani, Ilaria Rancan, Mario Tumbarello, Francesca Montagnani, Claudia Sala, Emanuele Montomoli, Rino Rappuoli
Summary: Research suggests that individuals who have been infected or vaccinated for the first time will produce antibodies with increased potency and breadth, allowing for better control of emerging SARS-CoV-2 variants.
Article
Biochemical Research Methods
Saki Horie, Kenji Mishiro, Mio Nishino, Inori Domae, Mitsuo Wakasugi, Tsukasa Matsunaga, Munetaka Kunishima
Summary: Developing a method for selective modification of antibodies while maintaining high affinity for the epitope is crucial for analytic, diagnostic, and therapeutic uses.
BIOCONJUGATE CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Miaomiao Li, Lars C. Pedersen, Ding Xu
Summary: Heparan sulfate-binding proteins (HSBPs) are structurally diverse extracellular and membrane attached proteins that interact with HS under normal physiological conditions. Interactions with HS offer an additional level of control over the localization and function of HSBPs. Manipulating HS-protein interactions could be explored as a therapeutic strategy to selectively antagonize/activate HSBPs.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Organic
Daniel Eszenyi, Attila Mandi, Mihaly Herczeg, Attila Benyei, Istvan Komaromi, Aniko Borbas
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Zsolt Szucs, Magdolna Csavas, Erzsebet Roth, Aniko Borbas, Gyula Batta, Florent Perret, Eszter Ostorhazi, Reka Szatmari, Evelien Vanderlinden, Lieve Naesens, Pal Herczegh
JOURNAL OF ANTIBIOTICS
(2017)
Article
Biotechnology & Applied Microbiology
Zsolt Szucs, Ilona Bereczki, Magdolna Csavas, Erzsebet Roth, Aniko Borbas, Gyula Batta, Eszter Ostorhazi, Reka Szatmari, Pal Herczegh
JOURNAL OF ANTIBIOTICS
(2017)
Article
Chemistry, Multidisciplinary
Laszlo Lazar, Laszlo Juhasz, Gyula Batta, Aniko Borbas, Laszlo Somsak
NEW JOURNAL OF CHEMISTRY
(2017)
Article
Chemistry, Organic
Adam Hadhazi, Mauro Pascolutti, Benjamin Bailly, Jeffrey C. Dyason, Aniko Borbas, Robin J. Thomson, Mark von Itzstein
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Magdolna Csavas, Daniel Eszenyi, Erika Mezo, Laszlo Lazar, Nora Debreczeni, Marietta Toth, Laszlo Somsak, Aniko Borbas
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Medicinal
Zsolt Szucs, Lieve Naesens, Annelies Stevaert, Eszter Ostorhazi, Gyula Batta, Pal Herczegh, Aniko Borbas
Article
Pharmacology & Pharmacy
Son Thai Le, David Pall, Erzsebet Roth, Tuyen Tran, Nora Debreczeni, Miklos Bege, Ilona Bereczki, Eszter Ostorhazi, Marton Milankovits, Pal Herczegh, Aniko Borbas, Magdolna Csavas
Summary: This study reports the first synthetic modifications of pleuromutilin and lefamulin at the alkene position C19-C20, using two different photoinduced addition reactions. The resulting semisynthetic pleuromutilin derivatives showed promising antimicrobial properties when tested on various bacterial strains.
Article
Chemistry, Medicinal
Ilona Bereczki, Henrietta Papp, Anett Kuczmog, Monika Madai, Veronika Nagy, Attila Agocs, Gyula Batta, Marton Milankovits, Eszter Ostorhazi, Janko Kos, Aron Zsigmond, Istvan Hajdu, David Bajusz, Jan Hodek, Jan Weber, Ferenc Jakab, Pal Herczegh, Aniko Borbas, Ana MitroviC, Zsolt Lorincz, Gyorgy Miklos Keserii
Summary: The prolonged global pandemic of COVID-19 has driven the need for the development of new drugs targeting SARS-CoV-2, with the glycopeptide antibiotic teicoplanin and certain apocarotenoids showing promising antiviral potential. The study suggests that these compounds could be further explored as potential antivirals against SARS-CoV-2.
Article
Chemistry, Medicinal
Ilona Bereczki, Zsolt Szucs, Gyula Batta, Tamas Milan Nagy, Eszter Ostorhazi, Katalin E. Kover, Aniko Borbas, Pal Herczegh
Summary: In order to enhance the antibacterial activity of teicoplanin against vancomycin-resistant enterococci, various dimeric derivatives were prepared. A cobalt coordination complex of a dimeric derivative showed high activity against VanA teicoplanin-resistant enterococci. Both covalent and complex dimeric derivatives exhibited significant activity against the resistant strain, but had no remarkable effect on other bacterial strains.
Review
Chemistry, Medicinal
Miklos Bege, Aniko Borbas
Summary: Nucleic acid medicines can achieve long-lasting or curative effects by targeting the genetic bases of diseases. However, the low in vivo stability and unfavorable physicochemical properties of native oligonucleotides hinder their therapeutic use. Synthetic oligonucleotides with proper chemical modifications have been developed to protect the nucleic acids from degradation, enable efficient cellular uptake, and target localization, ensuring the effectiveness of oligonucleotide-based therapy.
Article
Biochemistry & Molecular Biology
Miklos Bege, Alexandra Kiss, Ilona Bereczki, Jan Hodek, Lenke Polyak, Gabor Szeman-Nagy, Lieve Naesens, Jan Weber, Aniko Borbas
Summary: In this study, d-arabinofuranosyl-pyrimidine and -purine nucleoside analogues containing alkylthio-, acetylthio- or 1-thiosugar substituents at the C2' position were synthesized by photoinitiated, radical-mediated hydrothiolation reactions. Some of these compounds exhibited selective cytotoxicity against tumor cells and also showed antiviral activity against SARS-CoV-2 and/or HCoV-229E.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fruzsina Demeter, Patrik Toeroek, Alexandra Kiss, Richard Kovasznai-Olah, Zsuzsa Mathene Szigeti, Viktoria Baksa, Fruzsina Kovacs, Noemi Balla, Ferenc Fenyvesi, Judit Varadi, Aniko Borbas, Mihaly Herczeg
Summary: The emergence of drug-resistant bacteria and fungi is a serious global health issue. Cationic compounds can inhibit the growth of microorganisms by disrupting their cell membrane, and their use avoids the development of resistance. Researchers have designed novel carbohydrate-derived amidinium salts with quaternary ammonium groups, which can potentially disrupt the cell walls of bacteria and fungi. These compounds showed promising antimicrobial activity, particularly against Candida albicans, and their efficacy was influenced by the type of protecting groups and sugar configuration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kitti Szoke, Richard Kajtar, Alexandra Gyongyosi, Attila Czompa, Adina Fesus, Eszter Boglarka Lorincz, Ferenc Daniel Petroczi, Pal Herczegh, Istvan Bak, Aniko Borbas, Ilona Bereczki, Istvan Lekli
Summary: Research shows that chemical modification of cannabidiol (CBD) can overcome its low bioavailability and poor water solubility in therapeutic use. In this study, three new CBD derivatives were synthesized and found to have protective effects on cardiomyocytes exposed to oxidative stress and hypoxia/reoxygenation. The newly synthesized derivatives showed similar or better effects than CBD in decreasing cytotoxicity. CBD2 exhibited the strongest protective effect.
Article
Biochemistry & Molecular Biology
Magdolna Csavas, Lenka Malinovska, Florent Perret, Milan Gyurko, Zita Tunde Illyes, Michaela Wimmerova, Aniko Borbas
CARBOHYDRATE RESEARCH
(2017)
