期刊
CARBOHYDRATE RESEARCH
卷 344, 期 8, 页码 1046-1049出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2009.02.026
关键词
Aspergillus; Exo-beta-D-glucosaminidase; Gene cloning; His-Tag purification
资金
- NSFC [20576104]
- National High Technology Research and Development Progra (863 Program) of China [PCSIRT0627]
- 111 project [B07029]
- State Key Laboratory of Food Science and Technology, Jiangnan University [SKLF-MB-200805]
An exo-beta-D-glucosaminidase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an exo-chitosanase activity in a viscosimetric assay and TLC analysis. This is the first report on cloning of a gene encoding an Aspergillus sp. exo-beta-D-glucosaminidase. (C) 2009 Published by Elsevier Ltd.
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